LOCUS       prCAG-MS2               4103 bp ds-DNA     circular     09-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="47xCAG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_RNA        214..232
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_RNA        253..271
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_RNA        323..341
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_RNA        362..380
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_RNA        432..450
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_RNA        471..489
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_RNA        541..559
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_RNA        580..598
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_RNA        650..668
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_RNA        689..707
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_RNA        759..777
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_RNA        798..816
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    922..1510
                     /label="woodchuck hepatitis virus posttranscriptional regulatory element"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(1393..1404)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(1513..1526)
                     /label="common sequencing primer, one of multiple similar variants"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      1541..1669
                     /label="dblTerm"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(1597..1616)
                     /label="dblTer_R"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      1670..2387
                     /label="p15A"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            complement(2599..3258)
                     /label="CmR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            complement(3391..4017)
                     /label="TetR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     3898..3917
                     /label="TetR_F"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        4069..4087
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
       61 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
      121 cagcagcagc agcagcagca gagacggagc tcgtcgacgc ggccgcaagc ttggtaccga
      181 gctcggatcc taaggtacct aattgcctag aaaacatgag gatcacccat gtctgcaggt
      241 cgactctaga aaacatgagg atcacccatg tctgcagtat tcccgggttc attagatcct
      301 aaggtaccta attgcctaga aaacatgagg atcacccatg tctgcaggtc gactctagaa
      361 aacatgagga tcacccatgt ctgcagtatt cccgggttca ttagatccta aggtacctaa
      421 ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg actctagaaa acatgaggat
      481 cacccatgtc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
      541 acatgaggat cacccatgtc tgcaggtcga ctctagaaaa catgaggatc acccatgtct
      601 gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaaa catgaggatc
      661 acccatgtct gcaggtcgac tctagaaaac atgaggatca cccatgtctg cagtattccc
      721 gggttcatta gatcctaagg tacctaattg cctagaaaac atgaggatca cccatgtctg
      781 caggtcgact ctagaaaaca tgaggatcac ccatgtctgc agtattcccg ggttcattag
      841 atctgcgcgc gatcgatatc agcgctttaa atttgcgcat gctagtgtaa ggcatgcaag
      901 cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
      961 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     1021 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     1081 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     1141 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     1201 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     1261 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     1321 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     1381 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     1441 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     1501 gcctccccgc atcgataccg tcgaccTCGA GTAAGGATCT CCAGGCATCA AATAAAACGA
     1561 AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC
     1621 TACTAGAGTC ACACTGGCTC ACCTTCGGGT GGGCCTTTCT GCGTTTATAC CTAGGGATAT
     1681 ATTCCGCTTC CTCGCTCACT GACTCGCTAC GCTCGGTCGT TCGACTGCGG CGAGCGGAAA
     1741 TGGCTTACGA ACGGGGCGGA GATTTCCTGG AAGATGCCAG GAAGATACTT AACAGGGAAG
     1801 TGAGAGGGCC GCGGCAAAGC CGTTTTTCCA TAGGCTCCGC CCCCCTGACA AGCATCACGA
     1861 AATCTGACGC TCAAATCAGT GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1921 TCCCCCTGGC GGCTCCCTCG TGCGCTCTCC TGTTCCTGCC TTTCGGTTTA CCGGTGTCAT
     1981 TCCGCTGTTA TGGCCGCGTT TGTCTCATTC CACGCCTGAC ACTCAGTTCC GGGTAGGCAG
     2041 TTCGCTCCAA GCTGGACTGT ATGCACGAAC CCCCCGTTCA GTCCGACCGC TGCGCCTTAT
     2101 CCGGTAACTA TCGTCTTGAG TCCAACCCGG AAAGACATGC AAAAGCACCA CTGGCAGCAG
     2161 CCACTGGTAA TTGATTTAGA GGAGTTAGTC TTGAAGTCAT GCGCCGGTTA AGGCTAAACT
     2221 GAAAGGACAA GTTTTGGTGA CTGCGCTCCT CCAAGCCAGT TACCTCGGTT CAAAGAGTTG
     2281 GTAGCTCAGA GAACCTTCGA AAAACCGCCC TGCAAGGCGG TTTTTTCGTT TTCAGAGCAA
     2341 GAGATTACGC GCAGACCAAA ACGATCTCAA GAAGATCATC TTATTAATCA GATAAAATAT
     2401 TTCTAGATTT CAGTGCAATT TATCTCTTCA AATGTAGCAC CTGAAGTCAG CCCCATACGA
     2461 TATAAGTTGT TACTAGTGCT TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC
     2521 GTTCTGAACA AATCCAGATG GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA
     2581 AGCGAGCTCG ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC
     2641 ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG
     2701 CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA
     2761 GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC
     2821 TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA
     2881 ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT
     2941 CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT
     3001 ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT
     3061 CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT
     3121 CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA
     3181 CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC
     3241 AGTGATTTTT TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA
     3301 TACGCCCGGT AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC
     3361 AACGTCTCAT TTTCGCCAGA TATCGACGTC TTAAGACCCA CTTTCACATT TAAGTTGTTT
     3421 TTCTAATCCG CATATGATCA ATTCAAGGCC GAATAAGAAG GCTGGCTCTG CACCTTGGTG
     3481 ATCAAATAAT TCGATAGCTT GTCGTAATAA TGGCGGCATA CTATCAGTAG TAGGTGTTTC
     3541 CCTTTCTTCT TTAGCGACTT GATGCTCTTG ATCTTCCAAT ACGCAACCTA AAGTAAAATG
     3601 CCCCACAGCG CTGAGTGCAT ATAATGCATT CTCTAGTGAA AAACCTTGTT GGCATAAAAA
     3661 GGCTAATTGA TTTTCGAGAG TTTCATACTG TTTTTCTGTA GGCCGTGTAC CTAAATGTAC
     3721 TTTTGCTCCA TCGCGATGAC TTAGTAAAGC ACATCTAAAA CTTTTAGCGT TATTACGTAA
     3781 AAAATCTTGC CAGCTTTCCC CTTCTAAAGG GCAAAAGTGA GTATGGTGCC TATCTAACAT
     3841 CTCAATGGCT AAGGCGTCGA GCAAAGCCCG CTTATTTTTT ACATGCCAAT ACAATGTAGG
     3901 CTGCTCTACA CCTAGCTTCT GGGCGAGTTT ACGGGTTGTT AAACCTTCGA TTCCGACCTC
     3961 ATTAAGCAGC TCTAATGCGC TGTTAATCAC TTTACTTTTA TCTAATCTAG ACATCATTAA
     4021 TTCCTAATTT TTGTTGACAC TCTATCGTTG ATAGAGTTAT TTTACCACTC CCTATCAGTG
     4081 ATAGAGAAAA Gaattcggtc tca
//