LOCUS       prCAG-BoxB              3188 bp ds-DNA     circular     09-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="47xCAG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    183..201
                     /label="BoxB"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    227..245
                     /label="BoxB"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    251..269
                     /label="BoxB"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    295..313
                     /label="BoxB"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    319..337
                     /label="BoxB"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    363..381
                     /label="BoxB"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    409..427
                     /label="BoxB"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    452..470
                     /label="BoxB"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    476..494
                     /label="BoxB"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    520..538
                     /label="BoxB"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    544..562
                     /label="BoxB"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    588..606
                     /label="BoxB"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     terminator      626..754
                     /label="dblTerm"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(682..701)
                     /label="dblTer_R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      755..1472
                     /label="p15A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     gene            complement(1684..2343)
                     /label="CmR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     gene            complement(2476..3102)
                     /label="TetR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     2983..3002
                     /label="TetR_F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        3154..3172
                     /label="PLtetO-1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
       61 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
      121 cagcagcagc agcagcagca gAGACGGAGC TCGTCGACGC GGCCAAGCTT GGTACCGAGC
      181 TCGGGCCCTG AAGAAGGGCC CCTCGACTAA GTCCAACTAC TAAACTGGGC CCTGAAGAAG
      241 GGCCCATATA GGGCCCTGAA GAAGGGCCCC TCGACTAAGT CCAACTACTA AACTGGGCCC
      301 TGAAGAAGGG CCCATATAGG GCCCTGAAGA AGGGCCCCTC GACTAAGTCC AACTACTAAA
      361 CTGGGCCCTG AAGAAGGGCC CATATAGATC AAGCTTGGTA CCGAGCTCGG GCCCTGAAGA
      421 AGGGCCCCTC GACTAAGTCC ACTACTAAAC TGGGCCCTGA AGAAGGGCCC ATATAGGGCC
      481 CTGAAGAAGG GCCCCTCGAC TAAGTCCAAC TACTAAACTG GGCCCTGAAG AAGGGCCCAT
      541 ATAGGGCCCT GAAGAAGGGC CCCTCGACTA AGTCCAACTA CTAAACTGGG CCCTGAAGAA
      601 GGGCCCATAT ATCGAGTAAG GATCTCCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA
      661 GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT
      721 GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATACCTAGG GATATATTCC GCTTCCTCGC
      781 TCACTGACTC GCTACGCTCG GTCGTTCGAC TGCGGCGAGC GGAAATGGCT TACGAACGGG
      841 GCGGAGATTT CCTGGAAGAT GCCAGGAAGA TACTTAACAG GGAAGTGAGA GGGCCGCGGC
      901 AAAGCCGTTT TTCCATAGGC TCCGCCCCCC TGACAAGCAT CACGAAATCT GACGCTCAAA
      961 TCAGTGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGCGGCTC
     1021 CCTCGTGCGC TCTCCTGTTC CTGCCTTTCG GTTTACCGGT GTCATTCCGC TGTTATGGCC
     1081 GCGTTTGTCT CATTCCACGC CTGACACTCA GTTCCGGGTA GGCAGTTCGC TCCAAGCTGG
     1141 ACTGTATGCA CGAACCCCCC GTTCAGTCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     1201 TTGAGTCCAA CCCGGAAAGA CATGCAAAAG CACCACTGGC AGCAGCCACT GGTAATTGAT
     1261 TTAGAGGAGT TAGTCTTGAA GTCATGCGCC GGTTAAGGCT AAACTGAAAG GACAAGTTTT
     1321 GGTGACTGCG CTCCTCCAAG CCAGTTACCT CGGTTCAAAG AGTTGGTAGC TCAGAGAACC
     1381 TTCGAAAAAC CGCCCTGCAA GGCGGTTTTT TCGTTTTCAG AGCAAGAGAT TACGCGCAGA
     1441 CCAAAACGAT CTCAAGAAGA TCATCTTATT AATCAGATAA AATATTTCTA GATTTCAGTG
     1501 CAATTTATCT CTTCAAATGT AGCACCTGAA GTCAGCCCCA TACGATATAA GTTGTTACTA
     1561 GTGCTTGGAT TCTCACCAAT AAAAAACGCC CGGCGGCAAC CGAGCGTTCT GAACAAATCC
     1621 AGATGGAGTT CTGAGGTCAT TACTGGATCT ATCAACAGGA GTCCAAGCGA GCTCGATATC
     1681 AAATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC
     1741 GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT
     1801 GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT
     1861 GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT
     1921 ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG
     1981 CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA
     2041 CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG
     2101 CTCACCGTCT TTCATTGCCA TACGAAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT
     2161 GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT
     2221 AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA
     2281 ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC
     2341 CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC CCGGTAGTGA
     2401 TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CGATCAACGT CTCATTTTCG
     2461 CCAGATATCG ACGTCTTAAG ACCCACTTTC ACATTTAAGT TGTTTTTCTA ATCCGCATAT
     2521 GATCAATTCA AGGCCGAATA AGAAGGCTGG CTCTGCACCT TGGTGATCAA ATAATTCGAT
     2581 AGCTTGTCGT AATAATGGCG GCATACTATC AGTAGTAGGT GTTTCCCTTT CTTCTTTAGC
     2641 GACTTGATGC TCTTGATCTT CCAATACGCA ACCTAAAGTA AAATGCCCCA CAGCGCTGAG
     2701 TGCATATAAT GCATTCTCTA GTGAAAAACC TTGTTGGCAT AAAAAGGCTA ATTGATTTTC
     2761 GAGAGTTTCA TACTGTTTTT CTGTAGGCCG TGTACCTAAA TGTACTTTTG CTCCATCGCG
     2821 ATGACTTAGT AAAGCACATC TAAAACTTTT AGCGTTATTA CGTAAAAAAT CTTGCCAGCT
     2881 TTCCCCTTCT AAAGGGCAAA AGTGAGTATG GTGCCTATCT AACATCTCAA TGGCTAAGGC
     2941 GTCGAGCAAA GCCCGCTTAT TTTTTACATG CCAATACAAT GTAGGCTGCT CTACACCTAG
     3001 CTTCTGGGCG AGTTTACGGG TTGTTAAACC TTCGATTCCG ACCTCATTAA GCAGCTCTAA
     3061 TGCGCTGTTA ATCACTTTAC TTTTATCTAA TCTAGACATC ATTAATTCCT AATTTTTGTT
     3121 GACACTCTAT CGTTGATAGA GTTATTTTAC CACTCCCTAT CAGTGATAGA GAAAAGaatt
     3181 cggtctca
//