LOCUS       pMS2                    3962 bp ds-DNA     circular     26-FEB-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_RNA        73..91
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_RNA        112..130
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_RNA        182..200
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_RNA        221..239
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_RNA        291..309
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_RNA        330..348
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_RNA        400..418
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_RNA        439..457
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_RNA        509..527
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_RNA        548..566
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_RNA        618..636
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_RNA        657..675
                     /label="stem loop that binds the bacteriophage MS2 coat protein"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    781..1369
                     /label="woodchuck hepatitis virus posttranscriptional regulatory element"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(1252..1263)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     complement(1372..1385)
                     /label="common sequencing primer, one of multiple similar variants"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      1400..1528
                     /label="dblTerm"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(1456..1475)
                     /label="dblTer_R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      1529..2246
                     /label="p15A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     gene            complement(2458..3117)
                     /label="CmR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     gene            complement(3250..3876)
                     /label="TetR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     3757..3776
                     /label="TetR_F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        3928..3946
                     /label="PLtetO-1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 agacggagct cgtcgacgcg gccgcaagct tggtaccgag ctcggatcct aaggtaccta
       61 attgcctaga aaacatgagg atcacccatg tctgcaggtc gactctagaa aacatgagga
      121 tcacccatgt ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa
      181 aacatgagga tcacccatgt ctgcaggtcg actctagaaa acatgaggat cacccatgtc
      241 tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa acatgaggat
      301 cacccatgtc tgcaggtcga ctctagaaaa catgaggatc acccatgtct gcagtattcc
      361 cgggttcatt agatcctaag gtacctaatt gcctagaaaa catgaggatc acccatgtct
      421 gcaggtcgac tctagaaaac atgaggatca cccatgtctg cagtattccc gggttcatta
      481 gatcctaagg tacctaattg cctagaaaac atgaggatca cccatgtctg caggtcgact
      541 ctagaaaaca tgaggatcac ccatgtctgc agtattcccg ggttcattag atcctaaggt
      601 acctaattgc ctagaaaaca tgaggatcac ccatgtctgc aggtcgactc tagaaaacat
      661 gaggatcacc catgtctgca gtattcccgg gttcattaga tctgcgcgcg atcgatatca
      721 gcgctttaaa tttgcgcatg ctagtgtaag gcatgcaagc ttgatatcaa gcttatcgat
      781 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
      841 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
      901 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
      961 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     1021 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     1081 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     1141 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     1201 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     1261 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     1321 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     1381 cgaccTCGAG TAAGGATCTC CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG
     1441 GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCT ACTAGAGTCA CACTGGCTCA
     1501 CCTTCGGGTG GGCCTTTCTG CGTTTATACC TAGGGATATA TTCCGCTTCC TCGCTCACTG
     1561 ACTCGCTACG CTCGGTCGTT CGACTGCGGC GAGCGGAAAT GGCTTACGAA CGGGGCGGAG
     1621 ATTTCCTGGA AGATGCCAGG AAGATACTTA ACAGGGAAGT GAGAGGGCCG CGGCAAAGCC
     1681 GTTTTTCCAT AGGCTCCGCC CCCCTGACAA GCATCACGAA ATCTGACGCT CAAATCAGTG
     1741 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGCG GCTCCCTCGT
     1801 GCGCTCTCCT GTTCCTGCCT TTCGGTTTAC CGGTGTCATT CCGCTGTTAT GGCCGCGTTT
     1861 GTCTCATTCC ACGCCTGACA CTCAGTTCCG GGTAGGCAGT TCGCTCCAAG CTGGACTGTA
     1921 TGCACGAACC CCCCGTTCAG TCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
     1981 CCAACCCGGA AAGACATGCA AAAGCACCAC TGGCAGCAGC CACTGGTAAT TGATTTAGAG
     2041 GAGTTAGTCT TGAAGTCATG CGCCGGTTAA GGCTAAACTG AAAGGACAAG TTTTGGTGAC
     2101 TGCGCTCCTC CAAGCCAGTT ACCTCGGTTC AAAGAGTTGG TAGCTCAGAG AACCTTCGAA
     2161 AAACCGCCCT GCAAGGCGGT TTTTTCGTTT TCAGAGCAAG AGATTACGCG CAGACCAAAA
     2221 CGATCTCAAG AAGATCATCT TATTAATCAG ATAAAATATT TCTAGATTTC AGTGCAATTT
     2281 ATCTCTTCAA ATGTAGCACC TGAAGTCAGC CCCATACGAT ATAAGTTGTT ACTAGTGCTT
     2341 GGATTCTCAC CAATAAAAAA CGCCCGGCGG CAACCGAGCG TTCTGAACAA ATCCAGATGG
     2401 AGTTCTGAGG TCATTACTGG ATCTATCAAC AGGAGTCCAA GCGAGCTCGA TATCAAATTA
     2461 CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG
     2521 GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC
     2581 TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC
     2641 GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC
     2701 AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA
     2761 TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC
     2821 AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC
     2881 GTCTTTCATT GCCATACGAA ATTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT
     2941 AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC
     3001 CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC
     3061 TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATTTT
     3121 AGCTTCCTTA GCTCCTGAAA ATCTCGATAA CTCAAAAAAT ACGCCCGGTA GTGATCTTAT
     3181 TTCATTATGG TGAAAGTTGG AACCTCTTAC GTGCCGATCA ACGTCTCATT TTCGCCAGAT
     3241 ATCGACGTCT TAAGACCCAC TTTCACATTT AAGTTGTTTT TCTAATCCGC ATATGATCAA
     3301 TTCAAGGCCG AATAAGAAGG CTGGCTCTGC ACCTTGGTGA TCAAATAATT CGATAGCTTG
     3361 TCGTAATAAT GGCGGCATAC TATCAGTAGT AGGTGTTTCC CTTTCTTCTT TAGCGACTTG
     3421 ATGCTCTTGA TCTTCCAATA CGCAACCTAA AGTAAAATGC CCCACAGCGC TGAGTGCATA
     3481 TAATGCATTC TCTAGTGAAA AACCTTGTTG GCATAAAAAG GCTAATTGAT TTTCGAGAGT
     3541 TTCATACTGT TTTTCTGTAG GCCGTGTACC TAAATGTACT TTTGCTCCAT CGCGATGACT
     3601 TAGTAAAGCA CATCTAAAAC TTTTAGCGTT ATTACGTAAA AAATCTTGCC AGCTTTCCCC
     3661 TTCTAAAGGG CAAAAGTGAG TATGGTGCCT ATCTAACATC TCAATGGCTA AGGCGTCGAG
     3721 CAAAGCCCGC TTATTTTTTA CATGCCAATA CAATGTAGGC TGCTCTACAC CTAGCTTCTG
     3781 GGCGAGTTTA CGGGTTGTTA AACCTTCGAT TCCGACCTCA TTAAGCAGCT CTAATGCGCT
     3841 GTTAATCACT TTACTTTTAT CTAATCTAGA CATCATTAAT TCCTAATTTT TGTTGACACT
     3901 CTATCGTTGA TAGAGTTATT TTACCACTCC CTATCAGTGA TAGAGAAAAG aattcggtct
     3961 ca
//