LOCUS       pPN-mKate_(pCF.2)       4466 bp ds-DNA     circular     19-JAN-2021
DEFINITION  .
ACCESSION   urn.local...e-cwtxqms                                              
VERSION     urn.local...e-cwtxqms                                              
FEATURES             Location/Qualifiers
     misc_feature    2..322
                     /label="λ antitermination protein N"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    341..1039
                     /label="mKate"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     terminator      1079..1126
                     /label="T7\Terminator"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(1300..2088)
                     /label="aadA (SpR)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(2228..2965)
                     /label="CloDF13"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(3175..4257)
                     /label="lacI"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        4381..4397
                     /label="T7\Promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    4402..4422
                     /label="lacO"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     RBS             4455..4460
                     /label="RBS-1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 catggatgca caaacacgcc gtcgtgaacg tcgtgcggag aagcaggccc aatggaaggc
       61 agcaaatcct cttcttgtgg gggtgagtgc caaacctgtt aatcgtccta ttctttcgtt
      121 aaatcgcaag cctaagtctc gcgtggagtc cgcattgaac ccgattgatc ttactgtatt
      181 ggcggagtat cacaagcaga tcgaatctaa cctgcaacgt attgagcgca agaatcagcg
      241 cacctggtac tctaagccag gcgaacgcgg aatcacttgc agtggccgcc agaaaatcaa
      301 agggaagtca atcccactta ttggcggatc aggaggtggc atgtctgagc tgattaagga
      361 gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac
      421 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggcggtcga
      481 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa
      541 aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg
      601 cttcacatgg gagagagtca ccacatacga agatgggggc gtgctgaccg ctacccagga
      661 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc
      721 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagacgct
      781 gtaccccgct gacggcggcc tggaaggcag agccgacatg gccctgaagc tcgtgggcgg
      841 gggccacctg atctgcaact taaagaccac atacagatcc aagaaacccg ctaagaacct
      901 caagatgccc ggcgtctact atgtggacag acgactggaa agaatcaagg aggccgacaa
      961 agaaacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
     1021 actggggcac agatgataag aattcttaac ctaggctgct gccaccgctg agcaataact
     1081 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc
     1141 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     1201 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcat cgtggccgga
     1261 tcttgcggcc cctcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat
     1321 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1381 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1441 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1501 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1561 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1621 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1681 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1741 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1801 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1861 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1921 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     1981 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2041 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatac tcttcctttt
     2101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2161 tatttagaaa aataaacaaa tagctagctc actcggtcgc tacgctccgg gcgtgagact
     2221 gcggcgggcg ctgcggacac atacaaagta cccacagatt ccgtggataa gcaggggact
     2281 aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt tttccatagg ctccgccctc
     2341 ctgccagagt tcacataaac agacgctttt ccggtgcatc tgtgggagcc gtgaggctca
     2401 accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg
     2461 gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgttc
     2521 cgcctttctc ccttacggga agtgtggcgc tttctcatag ctcacacact ggtatctcgg
     2581 ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg ttcagcccga
     2641 ctgctgcgcc ttatccggta actgttcact tgagtccaac ccggaaaagc acggtaaaac
     2701 gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta gctaaacacg
     2761 cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga tttggttgct
     2821 gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt aaccttcgat
     2881 caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa
     2941 aggatctcaa gaagatcctt tgatcttttc tactgaaccg ctctagattt cagtgcaatt
     3001 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg
     3061 ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
     3121 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
     3181 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3241 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
     3301 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     3361 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     3421 gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc gcagcccgga
     3481 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     3541 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     3601 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     3661 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     3721 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
     3781 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     3841 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     3901 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     3961 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     4021 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     4081 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     4141 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     4201 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     4261 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
     4321 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaaat
     4381 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
     4441 tgtttaactt taataaggag atatac
//