LOCUS       placZα-tdMCP            3398 bp ds-DNA     circular     09-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Inducible promoter 23..76
                        /label="R0040"
                        /ApEinfo_revcolor="#f58a5e"
                        /ApEinfo_fwdcolor="#f58a5e"
     misc_feature       85..96
                        /label="B0034"
                        /ApEinfo_revcolor="#d6b295"
                        /ApEinfo_fwdcolor="#d6b295"
     CDS                103..423
                        /label="lacZ-alpha"
                        /ApEinfo_revcolor="#d59687"
                        /ApEinfo_fwdcolor="#d59687"
     CDS                103..423
                        /label="Translation 103-423"
     misc_feature       120..176
                        /label="MCS"
                        /ApEinfo_revcolor="#85dae9"
                        /ApEinfo_fwdcolor="#85dae9"
     primer_bind        complement(177..193)
                        /label="M13 fwd"
                        /ApEinfo_revcolor="#c7b0e3"
                        /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind        complement(177..194)
                        /label="M13 Forward"
                        /ApEinfo_revcolor="#b4abac"
                        /ApEinfo_fwdcolor="#b4abac"
     primer_bind        complement(186..208)
                        /label="M13/pUC Forward"
                        /ApEinfo_revcolor="#c7b0e3"
                        /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind        complement(402..421)
                        /label="pRS-marker"
                        /ApEinfo_revcolor="#b4abac"
                        /ApEinfo_fwdcolor="#b4abac"
     CDS                424..432
                        /label="Translation 424-432"
     misc_feature       433..436
                        /label="G2"
                        /ApEinfo_revcolor="#c6c9d1"
                        /ApEinfo_fwdcolor="#c6c9d1"
     CDS                433..453
                        /label="Translation 433-453"
     misc_feature       433..474
                        /label="linker"
                        /ApEinfo_revcolor="#9eafd2"
                        /ApEinfo_fwdcolor="#9eafd2"
     CDS                454..474
                        /label="Translation 454-474"
     misc_feature       475..1248
                        /label="dMCP_1"
                        /ApEinfo_revcolor="#c6c9d1"
                        /ApEinfo_fwdcolor="#c6c9d1"
     CDS                475..1248
                        /label="Translation 475-1248"
     CDS                1249..1254
                        /label="Translation 1249-1254"
     misc_feature       1255..1258
                        /label="G3"
                        /ApEinfo_revcolor="#ff9ccd"
                        /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature       complement(1420..1439)
                        /label="VR"
                        /ApEinfo_revcolor="#ffef86"
                        /ApEinfo_fwdcolor="#ffef86"
     misc_feature       complement(1526..2238)
                        /label="pMB1"
                        /ApEinfo_revcolor="#75c6a9"
                        /ApEinfo_fwdcolor="#75c6a9"
     CDS                complement(2298..2300)
                        /label="Translation 2300-2298"
     CDS                complement(2301..2960)
                        /label="AmpR"
                        /ApEinfo_revcolor="#ffef86"
                        /ApEinfo_fwdcolor="#ffef86"
     CDS                complement(2301..2960)
                        /label="Translation 2960-2301"
     misc_feature       complement(3159..3228)
                        /label="P(Bla)"
                        /ApEinfo_revcolor="#9eafd2"
                        /ApEinfo_fwdcolor="#9eafd2"
     misc_feature       3281..3300
                        /label="VF2"
                        /ApEinfo_revcolor="#ffef86"
                        /ApEinfo_fwdcolor="#ffef86"
     rrnC terminator    3357..3397
                        /label="B0062"
                        /ApEinfo_revcolor="#b7e6d7"
                        /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 GAATTCGCGG CCGCTTCTAG AGTCCCTATC AGTGATAGAG ATTGACATCC CTATCAGTGA
       61 TAGAGATACT GAGCACTACT AGAGAAAGAG GAGAAATACT AGatgaccat gattacgcca
      121 agcttgcatg catgcaggtc gacgctagag gatccccggg taccgagctc aaattcactg
      181 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      241 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      301 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
      361 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
      421 gcaGcggtct caACCGGAGG TGGTGGCAGT ACCAGCGGTG GAGGCGGTTC AACCATGGCA
      481 TCCAACTTCA CGCAATTCGT GTTAGTAGAC AATGGCGGCA CTGGGGATGT GACAGTGGCG
      541 CCCTCAAACT TTGCAAATGG TGTAGCAGAG TGGATTTCGA GTAATTCTCG CTCGCAAGCT
      601 TATAAAGTGA CTTGTTCGGT ACGTCAGAGC TCGGCCCAAA ATCGTAAATA TACGATCAAG
      661 GTCGAAGTAC CCAAAGTCGC GACTCAAACC GTAGGGGGCG TTGAGTTGCC TGTCGCCGCC
      721 TGGCGTAGTT ACCTTAATAT GGAGCTTACG ATTCCCATTT TTGCGACAAA TTCGGATTGC
      781 GAATTAATTG TGAAAGCCAT GCAAGGGCTG CTGAAGGACG GTAATCCCAT CCCGAGCGCA
      841 ATTGCCGCTA ACTCTGGCAT TTATGCGAAT TTTACCCAGT TTGTTTTGGT CGATAATGGT
      901 GGTACTGGtG AtGTTACTGT AGCGCCTTCA AACTTTGCGA ATGGAGTCGC AGAGTGGATC
      961 AGCTCGAACT CGCGCTCCCA AGCATATAAG GTGACGTGTA GTGTTCGTCA AAGCTCCGCG
     1021 CAGAACCGTA AGTACACTAT TAAAGTCGAG GTGCCCAAGG TCGCCACCCA GACGGTCGGA
     1081 GGGGTTGAAT TACCTGTAGC AGCCTGGCGT TCTTATCTTA ACATGGAGTT GACTATCCCT
     1141 ATCTTCGCTA CGAActctGA TTGTGAATTA ATCGTTAAGG CCATGCAAGG CCTGTTAAAG
     1201 GACGGAAATC CCATCCCCTC CGCTATTGCA GCCAATTCCG GCATCTACta ataaATTCtg
     1261 agacctacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc
     1321 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac
     1381 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1441 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1501 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
     1561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2161 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2281 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2341 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2401 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2461 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2521 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2581 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2641 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2701 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2761 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2821 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2881 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2941 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3001 ttaccgctgt tgagatccag ttcgatataa cccactcgtg cacccaactg atcttcagca
     3061 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3121 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3301 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
     3361 taaaaaaaat ccttagcttt cgctaaggat gatttctg
//