LOCUS       EK0294_SFFV-Che-SG(sarR 6591 bp ds-DNA     circular     29-AUG-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        6..499
                     /label="SFFV"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    74..74
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    102..102
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    180..180
                     /label="A->T from pSLQ2349"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    277..277
                     /label="G->A from pSLQ2349"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    505..512
                     /label="Kozak"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     CDS             513..1223
                     /label="Translation 513-1223"
     CDS             516..1223
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff4273
                     /ApEinfo_fwdcolor=#ff4273
     misc_feature    1224..1322
                     /label="5' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1224..1322
                     /label="Translation 1224-1322"
     CDS             1323..1328
                     /label="Translation 1323-1328"
     misc_feature    1329..1418
                     /label="synthetic arRNA.70587 sensor (UGG/W positive control)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1329..1418
                     /label="Translation 1329-1418"
     CDS             1419..1424
                     /label="Translation 1419-1424"
     misc_feature    1425..1523
                     /label="3' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1425..1523
                     /label="Translation 1425-1523"
     CDS             1524..2237
                     /label="GFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             1524..2237
                     /label="Translation 1524-2237"
     CDS             2238..2240
                     /label="Translation 2238-2240"
     polyA_signal    2343..2557
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     polyA_signal    2590..2613
                     /label="BGH pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    2896..2943
                     /label="FRT"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     gene            2951..3971
                     /label="Hygromycin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     polyA_signal    4103..4233
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     rep_origin      4616..5289
                     /label="pUC ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(5434..6294)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             complement(5434..6294)
                     /label="Translation 6294-5434"
     promoter        complement(6295..6393)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 aattgccgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
       61 ccacctgtag gttatggcaa gctagctgca gtaacgccat tattgcaagg catggaaaaa
      121 taccaaacca agaatagaga agttcagatc aagggcgggt acatgaaaat agctaacgta
      181 gggccaaaca ggatatctgc ggtgagcagt ttcggccccg gcccggggcc aagaacagat
      241 ggtcaccgca gtttcggccc cggcccgagg ccaagagcag atggtcccca gatatggccc
      301 aaccctcagc agtttcttaa gacccatcag atgtttccag gctcccccaa ggacctgaaa
      361 tgaccctgcg ccttatttga attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct
      421 tctgcttccc gagctctata aaagagctca caacccctca ctcggcgcgc cagtcctccg
      481 acagactgag tcgcccggga agcttcgcca ccatgGTGAG CAAGGGCGAG GAGGATAACA
      541 TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT GGAGGGCTCC GTGAACGGCC
      601 ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA
      661 AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT
      721 TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATTCCCGAC TACTTGAAGC
      781 TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG
      841 TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC
      901 GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG
      961 CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC
     1021 TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC AAGGCCAAGA
     1081 AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC ACCTCCCACA
     1141 ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC TCCACCGGCG
     1201 GCATGGACGA GCTGTACAAG AGCGAAGCCA GACACAAACA GAAAATTGTG GCTCCGGTGA
     1261 AACAGACTTT GAATTTTGAC CTTCTGAAGC TGGCGGGAGA CGTCGAGTCC AACCCCGGTC
     1321 CCggatccAC CAAGTCGACA CGGATGAGTG TTTCCTTCTA TCCGCGTCAT TGTTgGGCCT
     1381 TACGTACCGA TAGACCACTG GTGCAAATAG AGAGCTATac cggtGAGGCA CGGCATAAGC
     1441 AAAAGATCGT AGCCCCAGTA AAGCAAACAC TCAACTTCGA TCTACTCAAA CTCGCAGGTG
     1501 ATGTGGAATC TAATCCAGGA CCTGTCTCAA AGGGCGAGGA ACTTTTTACG GGCGTTGTTC
     1561 CGATCCTCGT CGAATTGGAC GGGGACGTTA ATGGGCATAA ATTCTCAGTT AGTGGTGAGG
     1621 GTGAGGGGGA CGCGACATAT GGGAAACTTA CGCTGAAGTT CATATGTACC ACCGGAAAAT
     1681 TGCCAGTGCC GTGGCCTACT CTCGTCACCA CCCTGACATA TGGTGTTCAG TGTTTTAGCC
     1741 GGTATCCAGA CCATATGAAA CAGCACGATT TTTTTAAATC CGCTATGCCT GAAGGTTACG
     1801 TACAGGAGCG AACGATATTT TTTAAAGACG ACGGAAACTA CAAAACTAGA GCCGAGGTAA
     1861 AATTCGAGGG TGACACTCTC GTGAACCGGA TCGAACTGAA GGGCATAGAT TTTAAGGAGG
     1921 ATGGCAATAT ACTGGGCCAC AAGCTCGAAT ACAACTATAA TTCTCATAAT GTCTATATCA
     1981 TGGCGGACAA ACAGAAAAAC GGCATCAAGG TAAACTTTAA AATCCGACAC AATATTGAGG
     2041 ACGGCTCTGT CCAGCTTGCG GACCATTACC AACAGAACAC GCCGATAGGG GATGGTCCAG
     2101 TACTTCTCCC TGATAATCAC TATTTGTCTA CTCAGTCAGC TCTTAGCAAG GACCCAAACG
     2161 AAAAACGGGA TCACATGGTC CTTCTTGAGT TCGTCACTGC GGCTGGGATA ACACTCGGGA
     2221 TGGACGAACT GTATAAGtag gccgctcgag gagggcccga acaaaaactc atctcagaag
     2281 aggatctgaa tagcgccgtc gaccatcatc ataatcatca ttgagtttaa acccgctgat
     2341 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     2401 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     2461 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     2521 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggagatct
     2581 atcgatgttc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     2641 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2701 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     2761 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggtccc
     2821 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     2881 tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     2941 ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     3001 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     3061 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     3121 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     3181 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     3241 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     3301 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     3361 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     3421 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     3481 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     3541 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     3601 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     3661 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     3721 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     3781 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     3841 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     3901 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     3961 ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt
     4021 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4081 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     4141 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4201 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     4261 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4321 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4381 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4441 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4501 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4561 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4621 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4681 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4741 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4801 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4861 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4921 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4981 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5041 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5101 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5161 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5221 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5281 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5341 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5401 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5461 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5521 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5581 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5641 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5701 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5761 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5821 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5881 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5941 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6001 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6061 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6121 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6181 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6241 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6301 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6361 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6421 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     6481 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     6541 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga c
//
