LOCUS       EK0290_SFFV-Che-SG(s705 6687 bp ds-DNA     circular     22-DEC-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        92..585
                     /label="SFFV"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    160..160
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    188..188
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    266..266
                     /label="A->T from pSLQ2349"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    363..363
                     /label="G->A from pSLQ2349"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    591..598
                     /label="Kozak"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     CDS             599..1309
                     /label="Translation 599-1309"
     CDS             602..1309
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff4273
                     /ApEinfo_fwdcolor=#ff4273
     misc_feature    1310..1408
                     /label="5' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1310..1408
                     /label="Translation 1310-1408"
     CDS             1409..1414
                     /label="Translation 1409-1414"
     misc_feature    1415..1504
                     /label="synthetic arRNA.70587 sensor"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1415..1504
                     /label="Translation 1415-1504"
     CDS             1505..1510
                     /label="Translation 1505-1510"
     misc_feature    1511..1600
                     /label="synthetic arRNA.17d9b sensor"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1511..1600
                     /label="Translation 1511-1600"
     CDS             1601..1606
                     /label="Translation 1601-1606"
     misc_feature    1607..1705
                     /label="3' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1607..1705
                     /label="Translation 1607-1705"
     CDS             1706..2419
                     /label="GFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             1706..2419
                     /label="Translation 1706-2419"
     CDS             2420..2422
                     /label="Translation 2420-2422"
     polyA_signal    2525..2739
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     polyA_signal    2772..2795
                     /label="BGH pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    3078..3125
                     /label="FRT"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     gene            3133..4153
                     /label="Hygromycin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     polyA_signal    4285..4415
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     rep_origin      4798..5471
                     /label="pUC ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(5616..6476)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             complement(5616..6476)
                     /label="Translation 6476-5616"
     promoter        complement(6477..6575)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
       61 ctacaacaag gcaaggcttg accgacaatt gccgataaaa taaaagattt tatttagtct
      121 ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta gctgcagtaa
      181 cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
      241 gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg agcagtttcg
      301 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
      361 gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
      421 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
      481 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
      541 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgggaagct tcgccaccat
      601 gGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
      661 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
      721 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
      781 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
      841 CGCCGACATT CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
      901 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
      961 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
     1021 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
     1081 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
     1141 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
     1201 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
     1261 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAGCG AAGCCAGACA
     1321 CAAACAGAAA ATTGTGGCTC CGGTGAAACA GACTTTGAAT TTTGACCTTC TGAAGCTGGC
     1381 GGGAGACGTC GAGTCCAACC CCGGTCCCgg atccACCAAG TCGACACGGA TGAGTGTTTC
     1441 CTTCTATCCG CGTCATTGTT aGGCCTTACG TACCGATAGA CCACTGGTGC AAATAGAGAG
     1501 CTATagatcc CGTCCGGACT CGAAAGGGAT GGTAGCACCC TGTCGAATCC GGCCTTaGAA
     1561 TGTCAGACGG TCGACCTACA TAATGAATCT CCTAAGCAGG accggtGAGG CACGGCATAA
     1621 GCAAAAGATC GTAGCCCCAG TAAAGCAAAC ACTCAACTTC GATCTACTCA AACTCGCAGG
     1681 TGATGTGGAA TCTAATCCAG GACCTGTCTC AAAGGGCGAG GAACTTTTTA CGGGCGTTGT
     1741 TCCGATCCTC GTCGAATTGG ACGGGGACGT TAATGGGCAT AAATTCTCAG TTAGTGGTGA
     1801 GGGTGAGGGG GACGCGACAT ATGGGAAACT TACGCTGAAG TTCATATGTA CCACCGGAAA
     1861 ATTGCCAGTG CCGTGGCCTA CTCTCGTCAC CACCCTGACA TATGGTGTTC AGTGTTTTAG
     1921 CCGGTATCCA GACCATATGA AACAGCACGA TTTTTTTAAA TCCGCTATGC CTGAAGGTTA
     1981 CGTACAGGAG CGAACGATAT TTTTTAAAGA CGACGGAAAC TACAAAACTA GAGCCGAGGT
     2041 AAAATTCGAG GGTGACACTC TCGTGAACCG GATCGAACTG AAGGGCATAG ATTTTAAGGA
     2101 GGATGGCAAT ATACTGGGCC ACAAGCTCGA ATACAACTAT AATTCTCATA ATGTCTATAT
     2161 CATGGCGGAC AAACAGAAAA ACGGCATCAA GGTAAACTTT AAAATCCGAC ACAATATTGA
     2221 GGACGGCTCT GTCCAGCTTG CGGACCATTA CCAACAGAAC ACGCCGATAG GGGATGGTCC
     2281 AGTACTTCTC CCTGATAATC ACTATTTGTC TACTCAGTCA GCTCTTAGCA AGGACCCAAA
     2341 CGAAAAACGG GATCACATGG TCCTTCTTGA GTTCGTCACT GCGGCTGGGA TAACACTCGG
     2401 GATGGACGAA CTGTATAAGt aggccgctcg aggagggccc gaacaaaaac tcatctcaga
     2461 agaggatctg aatagcgccg tcgaccatca tcataatcat cattgagttt aaacccgctg
     2521 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     2581 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     2641 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     2701 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggagat
     2761 ctatcgatgt tctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     2821 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggtc
     3001 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3061 gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga
     3121 acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     3181 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     3241 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     3301 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     3361 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     3421 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     3481 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     3541 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     3601 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     3661 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     3721 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     3781 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     3841 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     3901 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     3961 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     4021 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     4081 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     4141 agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4201 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4261 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     4321 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4381 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     4441 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4501 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4561 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4621 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4681 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4741 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4801 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4861 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4921 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4981 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5041 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5101 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5161 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5221 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5281 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5341 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5401 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5461 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5521 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5581 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5641 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5701 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5761 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5821 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5881 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5941 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6001 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6061 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6121 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6181 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6241 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6301 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6361 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6421 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6481 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6541 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6601 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     6661 tctgctctga tgccgcatag ttaagcc
//
