LOCUS       EK0304_CMV-TO-BFPmut-st 6027 bp ds-DNA     circular     10-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        88..715
                     /label="CMV"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     enhancer        88..814
                     /label="CMV promoter (with TetOs)"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     misc_feature    573..573
                     /label="A"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     TATA_signal     660..666
                     /label="TATA box"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    676..694
                     /label="TetO"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    676..715
                     /label="TetR binding sites"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    697..715
                     /label="TetO"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    839..846
                     /label="Kozak"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     CDS             847..849
                     /label="Translation 847-849"
     misc_feature    850..1557
                     /label="mtagBFP2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             850..1557
                     /label="Translation 850-1557"
     misc_feature    1501..1503
                     /label="E218A should be inactive (Subach 2010, modified for mTagBFP2 numbering)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1558..1560
                     /label="Translation 1558-1560"
     CDS             1561..1602
                     /label="Translation 1561-1602"
     misc_feature    1609..1614
                     /label="extra for cloning"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1615..1704
                     /label="Bdnf.t1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(1615..1704)
                     /label="Translation 1704-1615"
     misc_feature    1705..1710
                     /label="extra for cloning"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_signal    1801..2015
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     polyA_signal    2048..2071
                     /label="BGH pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    2354..2401
                     /label="FRT"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     gene            2409..3429
                     /label="Hygromycin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     polyA_signal    3561..3691
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     rep_origin      4074..4747
                     /label="pUC ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(4892..5752)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     promoter        complement(5753..5851)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
       61 gctgcttcgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
      121 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      181 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      241 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      301 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      481 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      541 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      601 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      661 atataagcag agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc
      721 gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      781 tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagcttc
      841 gccaccatgg tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac
      901 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
      961 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
     1021 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
     1081 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
     1141 acatacgagg acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
     1201 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
     1261 aagaaaacac tcggctggga ggccttcacc gaaacgctgt accccgctga cggcggcctg
     1321 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc
     1381 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
     1441 gtggactaca gactggaaag aatcaaggag gccaacaacg aaacctacgt cgagcagcac
     1501 gcggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaattga
     1561 agctagcTGG CTGGATACCC ATACGATGTT CCAGATTACG CTctcgagac cggtTAGTTC
     1621 CACCCGGAGT AGGGATGGAG AAAATTTCTT CACTATCCAT TCTGGTTGAT AAAGCGTTAC
     1681 ATTTGTATGT TGTAAAGATG TTTGggatcc gggcccgaac aaaaactcat ctcagaagag
     1741 gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttaaac ccgctgatca
     1801 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     1861 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     1921 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     1981 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggagatctat
     2041 cgatgttctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     2101 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2161 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2221 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggtccctt
     2281 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     2341 gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     2401 ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     2461 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     2521 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     2581 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     2641 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     2701 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     2761 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     2821 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     2881 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     2941 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     3001 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     3061 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     3121 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     3181 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     3241 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     3301 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     3361 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     3421 caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     3481 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     3541 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     3601 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3661 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     3721 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3781 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3841 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3901 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3961 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4561 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4741 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4801 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4861 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4921 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4981 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5041 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5101 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5161 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5221 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5281 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5341 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5401 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5461 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5521 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5581 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5641 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5701 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5761 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5821 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5881 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     5941 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     6001 gtagtgcgcg agcaaaattt aagctac
//
