LOCUS       EK0393_SFFV-mTagBFP2-SG 6600 bp ds-DNA     circular     22-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        92..585
                     /label="SFFV"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    160..160
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    188..188
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    266..266
                     /label="A->T from pSLQ2349"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    363..363
                     /label="G->A from pSLQ2349"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    591..598
                     /label="Kozak"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     CDS             599..601
                     /label="Translation 599-601"
     misc_feature    602..1309
                     /label="mTagBFP2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             602..1309
                     /label="Translation 602-1309"
     CDS             1310..1315
                     /label="Translation 1310-1315"
     misc_feature    1316..1414
                     /label="5' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1316..1414
                     /label="Translation 1316-1414"
     CDS             1415..1420
                     /label="Translation 1415-1420"
     CDS             1421..1426
                     /label="Translation 1421-1426"
     misc_feature    1427..1516
                     /label="EGFP.s2"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             1427..1516
                     /label="Translation 1427-1516"
     CDS             1517..1522
                     /label="Translation 1517-1522"
     CDS             1523..1528
                     /label="Translation 1523-1528"
     misc_feature    1529..1627
                     /label="3' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1529..1627
                     /label="Translation 1529-1627"
     CDS             1628..1633
                     /label="Translation 1628-1633"
     CDS             1634..2338
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff4273
                     /ApEinfo_fwdcolor=#ff4273
     CDS             1634..2338
                     /label="Translation 1634-2338"
     CDS             2339..2341
                     /label="Translation 2339-2341"
     polyA_signal    2438..2652
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     polyA_signal    2685..2708
                     /label="BGH pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    2991..3038
                     /label="FRT"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     gene            3046..4066
                     /label="Hygromycin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     polyA_signal    4198..4328
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     rep_origin      4711..5384
                     /label="pUC ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(5529..6389)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             complement(5529..6389)
                     /label="Translation 6389-5529"
     promoter        complement(6390..6488)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
       61 ctacaacaag gcaaggcttg accgacaatt gccgataaaa taaaagattt tatttagtct
      121 ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta gctgcagtaa
      181 cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
      241 gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg agcagtttcg
      301 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
      361 gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
      421 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
      481 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
      541 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgggaagct tcgccaccAT
      601 GGTGTCTAAG GGCGAAGAGC TGATTAAGGA GAACATGCAC ATGAAGCTGT ACATGGAGGG
      661 CACCGTGGAC AACCATCACT TCAAGTGCAC ATCCGAGGGC GAAGGCAAGC CCTACGAGGG
      721 CACCCAGACC ATGAGAATCA AGGTGGTCGA GGGCGGCCCT CTCCCCTTCG CCTTCGACAT
      781 CCTGGCTACT AGCTTCCTCT ACGGCAGCAA GACCTTCATC AACCACACCC AGGGCATCCC
      841 CGACTTCTTC AAGCAGTCCT TCCCTGAGGG CTTCACATGG GAGAGAGTCA CCACATACGA
      901 GGACGGGGGC GTGCTGACCG CTACCCAGGA CACCAGCCTC CAGGACGGCT GCCTCATCTA
      961 CAACGTCAAG ATCAGAGGGG TGAACTTCAC ATCCAACGGC CCTGTGATGC AGAAGAAAAC
     1021 ACTCGGCTGG GAGGCCTTCA CCGAAACGCT GTACCCCGCT GACGGCGGCC TGGAAGGCAG
     1081 AAACGACATG GCCCTGAAGC TCGTGGGCGG GAGCCATCTG ATCGCAAACG CCAAGACCAC
     1141 ATATAGATCC AAGAAACCCG CTAAGAACCT CAAGATGCCT GGCGTCTACT ATGTGGACTA
     1201 CAGACTGGAA AGAATCAAGG AGGCCAACAA CGAAACCTAC GTCGAGCAGC ACGAGGTGGC
     1261 AGTGGCCAGA TACTGCGACC TCCCTAGCAA ACTGGGGCAC AAACTTAACg cgttgGAAGC
     1321 CAGACACAAA CAGAAAATTG TGGCTCCGGT GAAACAGACT TTGAATTTTG ACCTTCTGAA
     1381 GCTGGCGGGA GACGTCGAGT CCAACCCCGG TCCCggtacc ggatccCGGC GGTCACGAAC
     1441 TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTaGGGTCTT TGCTCAGGGC GGACTGGGTG
     1501 CTCAGGTAGT GGTTGTaccg gtactagtGA GGCACGGCAT AAGCAAAAGA TCGTAGCCCC
     1561 AGTAAAGCAA ACACTCAACT TCGATCTACT CAAACTCGCA GGTGATGTGG AATCTAATCC
     1621 AGGACCCTCG AGTGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA AGGAGTTCAT
     1681 GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA
     1741 GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG
     1801 CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA
     1861 CGTGAAGCAC CCCGCCGACA TTCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA
     1921 GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC
     1981 CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA
     2041 CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC
     2101 CGAGGACGGC GCCCTGAAGG GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA
     2161 CTACGACGCT GAGGTCAAGA CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC
     2221 CTACAACGTC AACATCAAGT TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA
     2281 ACAGTACGAA CGCGCCGAGG GCCGCCACTC CACCGGCGGC ATGGACGAGC TGTACAAGta
     2341 ggccggaggg cccgaacaaa aactcatctc agaagaggat ctgaatagcg ccgtcgacca
     2401 tcatcataat catcattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     2461 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     2521 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     2581 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     2641 gcatgctggg gatgcggtgg gctctatgga gatctatcga tgttctgggg atgcggtggg
     2701 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     2761 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2821 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2881 cggctttccc cgtcaagctc taaatcgggg gtccctttag ggttccgatt tagtgcttta
     2941 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta gaagttccta
     3001 ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa gcctgaactc
     3061 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     3121 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     3181 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     3241 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     3301 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     3361 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     3421 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     3481 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     3541 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     3601 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     3661 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     3721 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     3781 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     3841 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     3901 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     3961 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     4021 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtactacga
     4081 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4141 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     4201 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4261 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4321 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     4381 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4441 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4501 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4561 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4621 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4681 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4741 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4801 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4861 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4921 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4981 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5041 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5101 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5161 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     5221 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5281 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5341 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5401 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5461 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5521 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5581 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5641 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5701 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5761 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5821 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5881 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5941 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6001 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6061 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6121 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6181 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6241 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6301 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6361 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6421 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6481 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     6541 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
//
