LOCUS       EK0423_SFFV-mTagBFP2-SG 6600 bp ds-DNA     circular     14-OCT-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        28..521
                     /label="SFFV"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    96..96
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    124..124
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    202..202
                     /label="A->T from pSLQ2349"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    299..299
                     /label="G->A from pSLQ2349"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    527..534
                     /label="Kozak"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     CDS             535..537
                     /label="Translation 535-537"
     misc_feature    538..1245
                     /label="mTagBFP2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             538..1245
                     /label="Translation 538-1245"
     CDS             1246..1251
                     /label="Translation 1246-1251"
     misc_feature    1252..1350
                     /label="5' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1252..1350
                     /label="Translation 1252-1350"
     CDS             1351..1356
                     /label="Translation 1351-1356"
     CDS             1357..1362
                     /label="Translation 1357-1362"
     misc_feature    1363..1452
                     /label="synthetic arRNA.70587 sensor"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1363..1452
                     /label="Translation 1363-1452"
     CDS             1453..1458
                     /label="Translation 1453-1458"
     CDS             1459..1464
                     /label="Translation 1459-1464"
     misc_feature    1465..1563
                     /label="3' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1465..1563
                     /label="Translation 1465-1563"
     CDS             1564..1569
                     /label="Translation 1564-1569"
     CDS             1570..2274
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff4273
                     /ApEinfo_fwdcolor=#ff4273
     CDS             1570..2274
                     /label="Translation 1570-2274"
     CDS             2275..2277
                     /label="Translation 2275-2277"
     polyA_signal    2374..2588
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     polyA_signal    2621..2644
                     /label="BGH pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    2927..2974
                     /label="FRT"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     gene            2982..4002
                     /label="Hygromycin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     polyA_signal    4134..4264
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     rep_origin      4647..5320
                     /label="pUC ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(5465..6325)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             complement(5465..6325)
                     /label="Translation 6325-5465"
     promoter        complement(6326..6424)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 aacaaggcaa ggcttgaccg acaattgccg ataaaataaa agattttatt tagtctccag
       61 aaaaaggggg gaatgaaaga ccccacctgt aggttatggc aagctagctg cagtaacgcc
      121 attattgcaa ggcatggaaa aataccaaac caagaataga gaagttcaga tcaagggcgg
      181 gtacatgaaa atagctaacg tagggccaaa caggatatct gcggtgagca gtttcggccc
      241 cggcccgggg ccaagaacag atggtcaccg cagtttcggc cccggcccga ggccaagagc
      301 agatggtccc cagatatggc ccaaccctca gcagtttctt aagacccatc agatgtttcc
      361 aggctccccc aaggacctga aatgaccctg cgccttattt gaattaacca atcagcctgc
      421 ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta taaaagagct cacaacccct
      481 cactcggcgc gccagtcctc cgacagactg agtcgcccgg gaagcttcgc caccATGGTG
      541 TCTAAGGGCG AAGAGCTGAT TAAGGAGAAC ATGCACATGA AGCTGTACAT GGAGGGCACC
      601 GTGGACAACC ATCACTTCAA GTGCACATCC GAGGGCGAAG GCAAGCCCTA CGAGGGCACC
      661 CAGACCATGA GAATCAAGGT GGTCGAGGGC GGCCCTCTCC CCTTCGCCTT CGACATCCTG
      721 GCTACTAGCT TCCTCTACGG CAGCAAGACC TTCATCAACC ACACCCAGGG CATCCCCGAC
      781 TTCTTCAAGC AGTCCTTCCC TGAGGGCTTC ACATGGGAGA GAGTCACCAC ATACGAGGAC
      841 GGGGGCGTGC TGACCGCTAC CCAGGACACC AGCCTCCAGG ACGGCTGCCT CATCTACAAC
      901 GTCAAGATCA GAGGGGTGAA CTTCACATCC AACGGCCCTG TGATGCAGAA GAAAACACTC
      961 GGCTGGGAGG CCTTCACCGA AACGCTGTAC CCCGCTGACG GCGGCCTGGA AGGCAGAAAC
     1021 GACATGGCCC TGAAGCTCGT GGGCGGGAGC CATCTGATCG CAAACGCCAA GACCACATAT
     1081 AGATCCAAGA AACCCGCTAA GAACCTCAAG ATGCCTGGCG TCTACTATGT GGACTACAGA
     1141 CTGGAAAGAA TCAAGGAGGC CAACAACGAA ACCTACGTCG AGCAGCACGA GGTGGCAGTG
     1201 GCCAGATACT GCGACCTCCC TAGCAAACTG GGGCACAAAC TTAACgcgtt gGAAGCCAGA
     1261 CACAAACAGA AAATTGTGGC TCCGGTGAAA CAGACTTTGA ATTTTGACCT TCTGAAGCTG
     1321 GCGGGAGACG TCGAGTCCAA CCCCGGTCCC ggtaccggat ccACCAAGTC GACACGGATG
     1381 AGTGTTTCCT TCTATCCGCG TCATTGTTaG GCCTTACGTA CCGATAGACC ACTGGTGCAA
     1441 ATAGAGAGCT ATaccggtac tagtGAGGCA CGGCATAAGC AAAAGATCGT AGCCCCAGTA
     1501 AAGCAAACAC TCAACTTCGA TCTACTCAAA CTCGCAGGTG ATGTGGAATC TAATCCAGGA
     1561 CCCTCGAGTG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC
     1621 TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC
     1681 GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC
     1741 CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG
     1801 AAGCACCCCG CCGACATTCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG
     1861 GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG
     1921 CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC
     1981 CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG
     2041 GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC
     2101 GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC
     2161 AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG
     2221 TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGtaggcc
     2281 ggagggcccg aacaaaaact catctcagaa gaggatctga atagcgccgt cgaccatcat
     2341 cataatcatc attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     2401 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     2461 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     2521 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     2581 gctggggatg cggtgggctc tatggagatc tatcgatgtt ctggggatgc ggtgggctct
     2641 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     2701 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2761 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2821 tttccccgtc aagctctaaa tcgggggtcc ctttagggtt ccgatttagt gctttacggc
     2881 acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag ttcctattcc
     2941 gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct gaactcaccg
     3001 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     3061 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     3121 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     3181 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     3241 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     3301 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     3361 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     3421 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     3481 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     3541 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     3601 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     3661 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     3721 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     3781 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     3841 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     3901 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     3961 gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta ctacgagatt
     4021 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     4081 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     4141 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4201 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4261 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     4321 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4381 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4441 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4501 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     5161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5761 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6061 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     6481 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     6541 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
//
