LOCUS       NSK025_CMV-TO-EGFP:c.63 8358 bp ds-DNA     circular     20-JAN-2022
DEFINITION  .
KEYWORDS    "accession:urn.local...1367881019035" "marker:5'TR, 3'TR, 6, 730 R,
            Xenopus globin 5'-UTR"
FEATURES             Location/Qualifiers
     promoter        1..628
                     /label="CMV"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     enhancer        1..727
                     /label="CMV promoter (with TetOs)"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     misc_feature    486..486
                     /label="A"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     TATA_signal     573..579
                     /label="TATA box"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    589..607
                     /label="TetO"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    589..628
                     /label="TetR binding sites"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    610..628
                     /label="TetO"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    752..759
                     /label="Kozak"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    760..1476
                     /label="EGFP (mainstream encoding)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             760..1476
                     /label="Translation 760-1476"
     misc_feature    1352..1441
                     /label="EGFP.t2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(1352..1441)
                     /label="Translation 1441-1352"
     misc_feature    1378..1395
                     /label="t"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1394..1396
                     /label="CCA>CCG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1397..1414
                     /label="t"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1473..1473
                     /label="C->T compared to LEAPER to remove Bsp1407I site"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             1477..1479
                     /label="Translation 1477-1479"
     CDS             1480..1521
                     /label="Translation 1480-1521"
     misc_feature    1528..1533
                     /label="extra for cloning"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1534..1623
                     /label="synthetic arRNA.70587"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1534..1623)
                     /label="Translation 1623-1534"
     misc_feature    1624..1629
                     /label="extra for cloning"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_signal    1728..1942
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1942..1943
                     /label="GATC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1944..1951
                     /label="NotI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /label="ES003-2 C->A"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1993..2301
                     /label="pSV40"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2358..3383
                     /label="Hygro"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3363..3383)
                     /label="6, 730 R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3384..3389
                     /label="SalI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3390..3494
                     /label="WPRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3500..3503
                     /label="SV40 pA(1)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3504..3507
                     /label="SV40 pA(1)"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    3505..3635
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3508..3508
                     /label="SV40 pA(1)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3509..3509
                     /label="SV40 pA(1)"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    3510..3635
                     /label="SV40 pA(1)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     enhancer        complement(3884..4114)
                     /label="core insul(1) Geneious name: core insul(1)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     repeat_region   4389..4423
                     /label="3'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4453..4453
                     /label="ES003-3 C->A"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6075..6935)
                     /label="Amp"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /label="5'TR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     repeat_region   7822..7852
                     /label="5'TR(1)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    7822..7852
                     /label="31bp spacer"
                     /ApEinfo_revcolor=#43ffb8
                     /ApEinfo_fwdcolor=#43ffb8
     repeat_region   7853..7854
                     /label="5'TR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     repeat_region   7856..7871
                     /label="5'TR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     enhancer        8047..8278
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    8161..8161
                     /label="ES003-1 C->A"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
                     /label="Gibson overlap"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    8292..8292
                     /label="Added MluI site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
      601 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
      661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      721 gcctccggac tctagcgttt aaacttaagc ttcgccaccA TGGTGAGCAA GGGCGAGGAG
      781 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG
      841 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC
      901 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC
      961 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC
     1021 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC
     1081 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG
     1141 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC
     1201 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG
     1261 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC
     1321 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC
     1381 CTGAGCAAAG ACCCCgACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC
     1441 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TAtAAGtaga gctagcTGGC TGGATACCCA
     1501 TACGATGTTC CAGATTACGC Tctcgagacc ggtATAGCTC TCTATTTGCA CCAGTGGTCT
     1561 ATCGGTACGT AAGGCCcAAC AATGACGCGG ATAGAAGGAA ACACTCATCC GTGTCGACTT
     1621 GGTggatccc tcgaggaggg cccgaacaaa aactcatctc agaagaggat ctgaatagcg
     1681 ccgtcgacca tcatcataat catcattgag tttaaacccg ctgatcagcc tcgactgtgc
     1741 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     1801 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     1861 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     1921 acaatagcag gcatgctggg gacgcggccg caatttaacg cgaattaatt ctgtggaatg
     1981 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2041 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2101 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     2161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2221 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     2281 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     2341 gatctgatca gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     2401 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     2461 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     2521 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     2581 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     2641 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     2701 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     2761 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     2821 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     2881 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     2941 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     3001 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     3061 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     3121 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     3181 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     3241 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     3301 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     3361 ctcgtccgag ggcaaaggaa taggtcgaca atcaacctct ggattacaaa atttgtgaaa
     3421 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3481 tgcctttgta tcatgcgtta actaaacttg tttattgcag cttataatgg ttacaaataa
     3541 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3601 ttgtccaaac tcatcaatgt atcttatcat gtctggaatt gactcaaatg atgtcaatta
     3661 gtctatcaga agctcatctg gtctcccttc cgggggacaa gacatccctg tttaatattt
     3721 aaacagcagt gttcccaaac tgggttctta tatcccttgc tctggtcaac caggttgcag
     3781 ggtttcctgt cctcacagga acgaagtccc taaagaaaca gtggcagcca ggtttagccc
     3841 cggaattgac tggattcctt ttttagggcc cattggtatg gctttttccc cgtatccccc
     3901 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
     3961 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc
     4021 ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt
     4081 acatccctgg gggctttggg ggggggctgt ccctgatatc tataacaaga aaatatatat
     4141 ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa
     4201 tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt
     4261 caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac
     4321 tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt
     4381 caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca
     4441 ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg
     4501 catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt
     4561 tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat
     4621 tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga
     4681 taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag
     4741 caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag
     4801 cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa
     4861 atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag
     4921 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4981 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5041 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5101 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5161 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5221 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5281 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5341 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5401 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5461 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5521 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5581 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5641 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5701 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     5761 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5821 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5881 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5941 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6001 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6061 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6121 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6181 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6241 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6301 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6361 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6421 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6481 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6541 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6601 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6661 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6721 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6781 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6841 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6901 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6961 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7021 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     7081 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     7141 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     7201 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     7261 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     7321 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     7381 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     7441 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     7501 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     7561 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     7621 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     7681 agcgcgcctc gttcattcac gtttttgaac ccgtggagga cgggcagact cgcggtgcaa
     7741 atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag aacacgcagc
     7801 tagattaacc ctagaaagat aatcatattg tgacgtacgt taaagataat catgtgtaaa
     7861 attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata
     7921 ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac aatttattta
     7981 tgtttattta tttattaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac
     8041 ttttatgagg gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc
     8101 tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc
     8161 cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc
     8221 tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaagg
     8281 cctccacggc cacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     8341 tacgggccag atatacgc
//
