LOCUS       EK0546_SFFV-mCherry-SGc 6686 bp ds-DNA     circular     02-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        92..585
                     /label="SFFV"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    160..160
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    188..188
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    266..266
                     /label="A->T from pSLQ2349"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    363..363
                     /label="G->A from pSLQ2349"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    591..598
                     /label="Kozak"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     CDS             599..601
                     /label="Translation 599-601"
     CDS             602..1309
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff4273
                     /ApEinfo_fwdcolor=#ff4273
     CDS             602..1309
                     /label="Translation 602-1309"
     CDS             1310..1318
                     /label="Translation 1310-1318"
     misc_feature    1319..1417
                     /label="5' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1319..1417
                     /label="Translation 1319-1417"
     CDS             1418..1423
                     /label="Translation 1418-1423"
     CDS             1424..1429
                     /label="Translation 1424-1429"
     CDS             1430..1522
                     /label="Translation 1430-1522"
     misc_feature    1430..1522
                     /label="sGAPDH.1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1475..1477
                     /label="stop to be edited by GAPDH 3' UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1523..1528
                     /label="Translation 1523-1528"
     CDS             1529..1534
                     /label="Translation 1529-1534"
     misc_feature    1535..1633
                     /label="3' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1535..1633
                     /label="Translation 1535-1633"
     CDS             1634..1639
                     /label="Translation 1634-1639"
     CDS             1640..2353
                     /label="GFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             1640..2353
                     /label="Translation 1640-2353"
     CDS             2354..2356
                     /label="Translation 2354-2356"
     misc_feature    2369..2434
                     /label="3xMS2 SGR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2378..2381
                     /label="loop MS2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2400..2403
                     /label="loop MS2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2422..2425
                     /label="loop MS2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2435..2438
                     /label="overhang"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     polyA_signal    2524..2738
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     polyA_signal    2771..2794
                     /label="BGH pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    3077..3124
                     /label="FRT"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     gene            3132..4152
                     /label="Hygromycin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     polyA_signal    4284..4414
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     rep_origin      4797..5470
                     /label="pUC ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(5615..6475)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             complement(5615..6475)
                     /label="Translation 6475-5615"
     promoter        complement(6476..6574)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
       61 ctacaacaag gcaaggcttg accgacaatt gccgataaaa taaaagattt tatttagtct
      121 ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta gctgcagtaa
      181 cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
      241 gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg agcagtttcg
      301 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
      361 gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
      421 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
      481 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
      541 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgggaagct tcgccaccAT
      601 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
      661 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
      721 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
      781 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
      841 CGCCGACATT CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
      901 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
      961 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
     1021 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
     1081 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
     1141 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
     1201 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
     1261 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAGCg acgcgttgGA
     1321 AGCCAGACAC AAACAGAAAA TTGTGGCTCC GGTGAAACAG ACTTTGAATT TTGACCTTCT
     1381 GAAGCTGGCG GGAGACGTCG AGTCCAACCC CGGTCCCggt accggatccC AAGGGGTCTA
     1441 CATGGCAACT GTGAGGAGGG GAGATTCAGT GTGGTaGTGA GGGACTGAGT GTGGCAGGGA
     1501 CTCCCCAGCA GTGAGGGTCT CTaccggtac tagtGAGGCA CGGCATAAGC AAAAGATCGT
     1561 AGCCCCAGTA AAGCAAACAC TCAACTTCGA TCTACTCAAA CTCGCAGGTG ATGTGGAATC
     1621 TAATCCAGGA CCCTCGAGTG TCTCAAAGGG CGAGGAACTT TTTACGGGCG TTGTTCCGAT
     1681 CCTCGTCGAA TTGGACGGGG ACGTTAATGG GCATAAATTC TCAGTTAGTG GTGAGGGTGA
     1741 GGGGGACGCG ACATATGGGA AACTTACGCT GAAGTTCATA TGTACCACCG GAAAATTGCC
     1801 AGTGCCGTGG CCTACTCTCG TCACCACCCT GACATATGGT GTTCAGTGTT TTAGCCGGTA
     1861 TCCAGACCAT ATGAAACAGC ACGATTTTTT TAAATCCGCT ATGCCTGAAG GTTACGTACA
     1921 GGAGCGAACG ATATTTTTTA AAGACGACGG AAACTACAAA ACTAGAGCCG AGGTAAAATT
     1981 CGAGGGTGAC ACTCTCGTGA ACCGGATCGA ACTGAAGGGC ATAGATTTTA AGGAGGATGG
     2041 CAATATACTG GGCCACAAGC TCGAATACAA CTATAATTCT CATAATGTCT ATATCATGGC
     2101 GGACAAACAG AAAAACGGCA TCAAGGTAAA CTTTAAAATC CGACACAATA TTGAGGACGG
     2161 CTCTGTCCAG CTTGCGGACC ATTACCAACA GAACACGCCG ATAGGGGATG GTCCAGTACT
     2221 TCTCCCTGAT AATCACTATT TGTCTACTCA GTCAGCTCTT AGCAAGGACC CAAACGAAAA
     2281 ACGGGATCAC ATGGTCCTTC TTGAGTTCGT CACTGCGGCT GGGATAACAC TCGGGATGGA
     2341 CGAACTGTAT AAGtaggccg gagggcccAC GTACACCATC AGGGTACGTC TCAGACACCA
     2401 TCAGGGTCTG TCTGGTACAG CATCAGCGTA CCATggcccg aacaaaaact catctcagaa
     2461 gaggatctga atagcgccgt cgaccatcat cataatcatc attgagttta aacccgctga
     2521 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     2581 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     2641 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     2701 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggagatc
     2761 tatcgatgtt ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2821 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2881 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2941 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggtcc
     3001 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3061 atggttcacg tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa
     3121 cttccttggc caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     3181 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     3241 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     3301 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     3361 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     3421 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     3481 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     3541 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     3601 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     3661 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     3721 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     3781 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     3841 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     3901 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     3961 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     4021 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     4081 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     4141 gggcaaagga atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     4201 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     4261 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     4321 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4381 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     4441 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4501 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4621 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5281 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6601 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     6661 ctgctctgat gccgcatagt taagcc
//
