LOCUS       Syn_Flex_FlpO           6173 bp DNA     circular UNA 18-NOV-2021
DEFINITION  Alignment of 2 sequences: Contig 2, Contig 1 (reversed).
ACCESSION   urn.local...34-e40q6n0
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2022 from SnapGene 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    complement(840..843)
                     /label=A
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; A 
                     (AAV2ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3; direction: LEFT"
     repeat_region   842..971
                     /label=AAV2 ITR
                     /note="color: #ffcc99"
     misc_feature    complement(844..859)
                     /label=RBE
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0, 
                     Adjusted-Interval-1:0/0; RBE (AAV2 ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(868..909)
                     /label=flop oriented DNA
                     /note="flop oriented DNA"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    869..887
                     /label=C arm
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3"
     misc_feature    complement(876..880)
                     /label=RBE'
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     RBE'(AAV2 ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    891..910
                     /label=B arm
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3"
     misc_feature    919..934
                     /label=RBE
                     /note="/created_by=lemirea"
                     /note="color: #a6acb3"
     misc_feature    935..951
                     /label=A
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; A' 
                     (AAV2ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3"
     regulatory      950..951
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     TRS(AAV2 ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="/label=TRS"
                     /note="/modified_by=lemirea"
                     /note="color: #ffffff"
     misc_feature    952..971
                     /label=D
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; D 
                     (AAV2ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3"
     misc_feature    1031..1049
                     /label=TCTGCAGAGGGCCCTGCGT
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=TCTGCAGAGGGCCCTGCGT"
                     /note="color: #a6acb3"
     misc_feature    1032..1503
                     /label=synapsin promoter
                     /note="synapsin promoter"
                     /note="color: #800080"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="/Primary_Match=90.25%; Adjusted-Interval-0:0/0; 
                     synapsin promoter"
                     /note="/Transferred_From=synapsin promoter"
                     /note="/Transferred_Similarity=90.25%"
                     /note="color: #a6acb3"
     misc_feature    1061..1102
                     /label=TGGGTTTTAGGACCAGGATGAGGCGGGGTGGGGGTGCCTACC
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=TGGGTTTTAGGACCAGGATGAGGCGGGGTGGGGGTG
                     CCTACC"
                     /note="color: #a6acb3"
     intron          1593..1725
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
                     /note="color: #------"
     protein_bind    complement(1738..1771)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with 
                     eachother, but incompatible with loxP or loxN sites."
                     /note="Derived using Geneious Prime 2021.2.2 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     lox2272"
                     /note="/Transferred_From=lox2272"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(1834..1867)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     gene            complement(1834..1867)
                     /label=loxP
                     /note="Derived using Geneious Prime 2021.2.2 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     loxP"
                     /note="/Transferred_From=loxP"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(1910..3181)
                     /product="FLPo"
                     /label=FLPo CDS
                     /note="Derived using Geneious Prime 2021.2.2 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=98.09%; Adjusted-Interval-0:0/0; FLPo
                     CDS(KX151728 - FLPo CDS (reversed))"
                     /note="/Transferred_From=KX151728 - FLPo CDS (reversed)"
                     /note="/Transferred_Similarity=98.09%"
                     /note="/Transferred_Translation=MAPKKKRKVMSQFDILCKTPPKVLVRQ
                     FVERF 
                     ERPSGEKIASCAAELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKY 
                     KTQKATILEASLKKLIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSH 
                     SKKMLKALLSEGESIWEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKN 
                     VDPKSFKLVQNKYLGVIIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPV 
                     LKRVNRTGNSSSNKQEYQLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTS 
                     FLSMKGLTELTNVVGNWSDKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEM 
                     IALKDETNPIEEWQHIEQLKGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
                     /note="color: #993366; direction: LEFT"
                     /db_xref="GI:1047836618"
                     /protein_id="ANW61888.1"
     misc_feature    complement(1910..3181)
                     /label=ORF 37 (frame 1)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/created_by=ORF Finder"
                     /note="color: #a6acb3; direction: LEFT"
     protein_bind    3214..3247
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with 
                     eachother, but incompatible with loxP or loxN sites."
                     /note="Derived using Geneious Prime 2021.2.2 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     lox2272"
                     /note="/Transferred_From=lox2272"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #31849b"
     protein_bind    3318..3351
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    3423..4011
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(3894..3905)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3893"
                     /translation="IEGR"
     polyA_signal    4089..4210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     terminator      4318
                     /label=SV40_PA term
                     /note="Derived using Geneious Prime 2021.2.2 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Primary_Match=88.21%; Adjusted-Interval-0:0/0; 
                     SV40_PA term"
                     /note="/Transferred_From=SV40_PA term"
                     /note="/Transferred_Similarity=88.21%"
                     /note="/modified_by=lemirea"
                     /note="color: #ffffff"
     repeat_region   4372..4501
                     /label=AAV2 ITR
                     /note="color: #ffcc99"
     misc_feature    complement(4372..4391)
                     /label=D
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; D 
                     (AAV2ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(4392..4408)
                     /label=A
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; A' 
                     (AAV2ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3; direction: LEFT"
     regulatory      4392..4393
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     TRS(AAV2 ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="/label=TRS"
                     /note="/modified_by=lemirea"
                     /note="color: #ffffff"
     misc_feature    complement(4409..4424)
                     /label=RBE
                     /note="/created_by=lemirea"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4433..4452
                     /label=B arm
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3"
     misc_feature    4434..4475
                     /label=flop oriented DNA
                     /note="flop oriented DNA"
                     /note="color: #a6acb3"
     misc_feature    4456..4474
                     /label=C arm
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3"
     misc_feature    4463..4467
                     /label=RBE'
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     RBE'(AAV2 ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="color: #a6acb3"
     misc_feature    4484..4499
                     /label=RBE
                     /note="Derived using Geneious Prime 2020.1.1 'Transfer 
                     Annotations' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0, 
                     Adjusted-Interval-1:0/0; RBE (AAV2 ITR (flip orientation))"
                     /note="/Transferred_From=AAV2 ITR (flip orientation)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=lemirea"
                     /note="/modified_by=lemirea"
                     /note="color: #a6acb3"
     rep_origin      4573..5028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5054..5158
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        5089..5117
                     /label=amp prom
                     /note="Derived using Geneious Prime 2021.2.2 'Annotatefrom 
                     Database' based on nucleotide similarity"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; amp
                     prom"
                     /note="/Transferred_From=amp prom"
                     /note="/Transferred_Similarity=100.00%"
                     /note="color: #ffffff"
     CDS             5159..6019
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5159 .. 5227 / #ccffcc / signal sequence
                        2: 5228 .. 6019 / #ccffcc
                     Cleavage site after base 5227"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgcc
      841 tctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
      901 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      961 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
     1021 taggaagatc cgagctccag tgtgctggaa tgagtgcaag gagctccagt gtgctggaat
     1081 tcgccctttt cagtatttaa attgacgacc gaccccgacc cactggacaa gcacccaacc
     1141 cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag gatgcggcga
     1201 ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc gcctggcggc
     1261 gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg tcccccgcaa
     1321 actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac
     1381 cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac
     1441 tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag
     1501 ctgtgctcct gggcaccgcg cagtccgccc ccgcggctcc tggccagacc acccctagga
     1561 ccccctgccc caagtcgcag ccgttggatc aggtaagtat caaggttaca agacaggttt
     1621 aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
     1681 cacctattgg tcttactgac atccactttg cctttctctc cacaggtgat gatccccata
     1741 acttcgtata aagtatccta tacgaagtta tatcaaaata ggaagaccaa tgcttcacca
     1801 tcgacccgaa ttgccaagca tcaccatcga cacataactt cgtataatgt atgctatacg
     1861 aagttatact agtgctagct gatcaacgcg tcttaaggat atcaccggtt cagatccgcc
     1921 tgttgatgta gctgctcagg tagtccagca cctcctggct gatgatgccg ttccaggcgg
     1981 ggtatctgat gctgccctcg gcgctgccct tcagctgctc gatgtgctgc cactcctcga
     2041 tggggttggt ctcgtccttc agggcgatca tctccttgct gatggggtcg taggcgtagt
     2101 acctggacac cagggcgaag tagtggtcgg ggatggcggt gatctggtgg gtgtaggtgg
     2161 tcctggccac ggcggaggcc ctcttgtcgc tccagttgcc caccacgttt gtcagctcgg
     2221 tcaggccctt catgctcaga aagctggtca tcaggtgcct gccgatgtgg ctcttagggc
     2281 cgttcttgat agcgaagatg gggtaggggg cgttcttctt cagggccttg ttgtagctgc
     2341 gcaccaggtt gtccttcagc agctggtact cctgcttgtt gctgctgctg ttgccggtcc
     2401 tgttcactct cttcagcacg ggctcgctgt tcctcaggaa ctcgtccagg tacaccaggg
     2461 ggtcgatcct gcctctggcg ctgaaaaagt agatgtgcct ggacacgctt gtcttggtct
     2521 cggtcaccag gcactgaatg atcacgccca ggtacttgtt ctgcaccagc ttgaagctct
     2581 tggggtccac gttcttgatg tcgctgaacc tgccgcagtt gatgaatgtg gccaggaaca
     2641 ggaactggta cagggtcttg gtcttggtga acctgctggt gtactcgaag ctgttcagga
     2701 tcttctcggt gatctcccag atgctctcgc cctcggacag cagggccttc agcatcttct
     2761 tgctgtggct gttgcccttg tcggcctcct cgctgctctc gaactgcagc tgcaggctgg
     2821 acacgatgtc ggtgatgtcg ctctggtgct tctggccgtt gtaagggatg atggtgaact
     2881 cccaggcggg gatcagcttc ttcaggctgg cctccaggat ggtggccttc tgggtcttgt
     2941 acttgaactg caggctcttg ttcacgatgt cgaagctcag gctgttgctg atgatggtgt
     3001 tgtagctcat gaaggtggcc ctcttgatgg cggtgccgtt gtgggtgatc atccagcaca
     3061 ggtaggtcag ctcggcggca cagctggcga tcttctcgcc gctgggcctc tcgaatctct
     3121 ccacgaactg ccgcaccagc accttggggg gggtcttgca caggatgtcg aactggctca
     3181 tggtggcgga tccggcgcgc ccatatgact agtataactt cgtataggat actttatacg
     3241 aagttatcat tgggattctt cctattttga tccaagcatc accatcgacc ctctagtcca
     3301 gatctcacca tcgacccata acttcgtata gcatacatta tacgaagtta tgtccctcga
     3361 agaggttcga aacactgaaa agggcgaatt ctgcagatga tccactcgag aagcttatcg
     3421 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3481 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3541 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3601 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3661 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3721 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3781 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     3841 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3901 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3961 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     4021 gtcgacccgg gcggccgctc gagcatgcat ctagagtcga cccgggcggc cgcttcgagc
     4081 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4141 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4201 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     4261 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatcttcc
     4321 tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac aaggaacccc
     4381 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4441 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     4501 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4561 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4621 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4681 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4741 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4801 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4861 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4921 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4981 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     5041 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5101 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5161 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5221 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5281 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5341 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5401 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5461 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5521 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5581 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5641 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5701 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5761 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5821 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5881 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5941 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6001 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6061 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaa
//
