LOCUS       Exported                9159 bp ds-DNA     circular SYN 31-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCAG-AsCas12a-Plus-mChery
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Huang HX
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 31, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     7..1725
                     /label=CAG
     misc_feature    7
                     /label=CAG start site
     primer_bind     complement(171..191)
                     /label=CAGR2
     misc_feature    1609..1627
                     /label=CP5
     misc_feature    1771..5691
                     /label=RKA
     misc_feature    2431..2448
                     /label=174-F
     misc_feature    3225..3243
                     /label=3200-F
     misc_feature    4127..4146
                     /label=As-4130-Fce
     misc_feature    4472..4489
                     /label=PmI l-R
     misc_feature    5014..5033
                     /label=As-5000-Fce
     misc_feature    5692..5739
                     /label=NLS
     misc_feature    5746..5826
                     /label=3xHA
     misc_feature    5833..5835
                     /label=Ser
     misc_feature    5842..5907
                     /label=GSG-P2A
     misc_feature    5867..5885
                     /label=P2A-Fce
     CDS             5914..6621
                     /codon_start=1
                     /label=mChery
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    6667..6788
                     /label=SV40 pA
     primer_bind     complement(6837..6853)
                     /label=M13R
     oriT            7239..7827
                     /direction=RIGHT
                     /label=ori
     CDS             complement(8001..8858)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8859..8963)
                     /label=pAmpR
     primer_bind     9097..9116
                     /label=MfeI-seqF
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatgggtc gaggtgagcc ccacgttctg cttcactctc
      421 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
      481 gcagcgatgg gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgtccg
     1561 cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagtgcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt catttaggtg
     1741 acactataga taatacgact cactataggg atgacacagt tcgagggctt taccaacctg
     1801 tatcaggtga gcaagacact gcggtttgag ctgatcccac agggcaagac cctgaagcac
     1861 atccaggagc agggcttcat cgaggaggac aaggcccgca atgatcacta caaggagctg
     1921 aagcccatca tcgatcggat ctacaagacc tatgccgacc agtgcctgca gctggtgcag
     1981 ctggattggg agaacctgag cgccgccatc gactcctata gaaaggagaa aaccgaggag
     2041 acaaggaacg ccctgatcga ggagcaggcc acatatcgca atgccatcca cgactacttc
     2101 atcggccgga cagacaacct gaccgatgcc atcaataaga gacacgccga gatctacaag
     2161 ggcctgttca aggccgagct gtttaatggc aaggtgctga agcagctggg caccgtgacc
     2221 acaaccgagc acgagaacgc cctgctgcgg agcttcgaca agtttacaac ctacttctcc
     2281 ggcttttata gaaacaggaa gaacgtgttc agcgccgagg acattagcac agccatccca
     2341 caccgcatcg tgcaggacaa cttccccaag tttaaggaga attgtcacat cttcacacgc
     2401 ctgatcaccg ccgtgcccag cctgcgggag cactttgaga acgtgaagaa ggccatcggc
     2461 atcttcgtga gcacctccat cgaggaggtg ttttccttcc ctttttataa ccagctgctg
     2521 acacagaccc agatcgacct gtataaccag ctgctgggag gaatctctcg ggaggcaggc
     2581 accgagaaga tcaagggcct gaacgaggtg ctgaatctgg ccatccagaa gaatgatgag
     2641 acagcccaca tcatcgcctc cctgccacac agattcatcc ccctgtttaa gcagatcctg
     2701 tccgatagga acaccctgtc tttcatcctg gaggagttta agagcgacga ggaagtgatc
     2761 cagtccttct gcaagtacaa gacactgctg agaaacgaga acgtgctgga gacagccgag
     2821 gccctgttta acgagctgaa cagcatcgac ctgacacaca tcttcatcag ccacaagaag
     2881 ctggagacaa tcagcagcgc cctgtgcgac cactgggata cactgaggaa tgccctgtat
     2941 gagcggagaa tctccgagct gacaggcaag atcaccaagt ctgccaagga gaaggtgcag
     3001 cgcagcctga agcacgagga tatcaacctg caggagatca tctctgccgc aggcaaggag
     3061 ctgagcgagg ccttcaagca gaaaaccagc gagatcctgt cccacgcaca cgccgccctg
     3121 gatcagccac tgcctacaac cctgaagaag caggaggaga aggagatcct gaagtctcag
     3181 ctggacagcc tgctgggcct gtaccacctg ctggactggt ttgccgtgga tgagtccaac
     3241 gaggtggacc ccgagttctc tgcccggctg accggcatca agctggagat ggagccttct
     3301 ctgagcttct acaacaaggc cagaaattat gccaccaaga agccctactc cgtggagaag
     3361 ttcaagctga actttcagat gcctacactg gcctctggct gggacgtgaa taaggagaag
     3421 aacaatggcg ccatcctgtt tgtgaagaac ggcctgtact atctgggcat catgccaaag
     3481 cagaagggca ggtataaggc cctgagcttc gagcccacag agaaaaccag cgagggcttt
     3541 gataagatgt actatgacta cttccctgat gccgccaaga tgatcccaaa gtgcagcacc
     3601 cagctgaagg ccgtgacagc ccactttcag acccacacaa cccccatcct gctgtccaac
     3661 aatttcatcg agcctctgga gatcacaaag gagatctacg acctgaacaa tcctgagaag
     3721 gagccaaaga agtttcagac agcctacgcc aagaaaaccg gcgaccagaa gggctacaga
     3781 gaggccctgt gcaagtggat cgacttcaca agggattttc tgtccaagta taccaagaca
     3841 acctctatcg atctgtctag cctgcggcca tcctctcagt ataaggacct gggcgagtac
     3901 tatgccgagc tgaatcccct gctgtaccac atcagcttcc agagaatcgc cgagaaggag
     3961 atcatggatg ccgtggagac aggcaagctg tacctgttcc agatctataa caaggacttt
     4021 gccaagggcc accacggcaa gcctaatctg cacacactgt attggaccgg cctgttttct
     4081 ccagagaacc tggccaagac aagcatcaag ctgaatggcc aggccgagct gttctaccgc
     4141 cctaagtcca ggatgaagag gatggcacac cggctgggag agaagatgct gaacaagaag
     4201 ctgaaggatc agaaaacccc aatccccgac accctgtacc aggagctgta cgactatgtg
     4261 aatcacagac tgtcccacga cctgtctgat gaggccaggg ccctgctgcc caacgtgatc
     4321 accaaggagg tgtctcacga gatcatcaag gataggcgct ttaccagcga caagttcttt
     4381 ttccacgtgc ctatcacact gaactatcag gccgccaatt ccccatctaa gttcaaccag
     4441 agggtgaatg cctacctgaa ggagcacccc gagacaccta tcatcggcat cgatcggggc
     4501 gagagaaacc tgatctatat cacagtgatc gactccaccg gcaagatcct ggagcagcgg
     4561 agcctgaaca ccatccagca gtttgattac cagaagaagc tggacaacag ggagaaggag
     4621 aaggtggcgg ccgctcaggc ctggtctgtg gtgggcacaa tcaaggatct gaagcagggc
     4681 tatctgagcc aggtcatcca cgagatcgtg gacctgatga tccactacca ggccgtggtg
     4741 gtgctggaga acctgaattt cggctttaag agcaagagga ccggcatcgc cgagaaggcc
     4801 gtgtaccagc agttcgagaa gatgctgatc gataagctga attgcctggt gctgaaggac
     4861 tatccagcag agaaagtggg aggcgtgctg aacccatacc agctgacaga ccagttcacc
     4921 tcctttgcca agatgggcac ccagtctggc ttcctgtttt acgtgcctgc cccatataca
     4981 tctaagatcg atcccctgac cggcttcgtg gaccccttcg tgtggaaaac catcaagaat
     5041 cacgagagcc gcaagcactt cctggagggc ttcgactttc tgcactacga cgtgaaaacc
     5101 ggcgacttca tcctgcactt taagatgaac agaaatctgt ccttccagag gggcctgccc
     5161 ggctttatgc ctgcatggga tatcgtgttc gagaagaacg agacacagtt tgacgccaag
     5221 ggcacccctt tcatcgccgg caagagaatc gtgccagtga tcgagaatca cagattcacc
     5281 ggcagatacc gggacctgta tcctgccaac gagctgatcg ccctgctgga ggagaagggc
     5341 atcgtgttca gggatggctc caacatcctg ccaaagctgc tggagaatga cgattctcac
     5401 gccatcgaca ccatggtggc cctgatccgc agcgtgctgc agatgcggaa ctccaatgcc
     5461 gccacaggcg aggactatat caacagcccc gtgcgcgatc tgaatggcgt gtgcttcgac
     5521 tcccggtttc agaacccaga gtggcccatg gacgccgatg ccaatggcgc ctaccacatc
     5581 gccctgaagg gccagctgct gctgaatcac ctgaaggaga gcaaggatct gaagctgcag
     5641 aacggcatct ccaatcagga ctggctggcc tacatccagg agctgcgcaa caaaaggccg
     5701 gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg gatcctaccc atacgatgtt
     5761 ccagattacg cttatcccta cgacgtgcct gattatgcat acccatacga tgtccccgac
     5821 tatgccctcg agagcaccgg tggcagcgga gctactaact tcagcctgct gaagcaggct
     5881 ggagacgtgg aggagaaccc tggacctgcc ggtatggtga gcaagggcga ggaggataac
     5941 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     6001 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     6061 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     6121 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     6181 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     6241 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     6301 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     6361 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     6421 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     6481 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     6541 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     6601 ggcatggacg agctgtacaa gtaactgcag cgcggggatc tcatgctgga gttcttcgcc
     6661 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6721 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6781 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6841 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6901 agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6961 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7021 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7141 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7921 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7981 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8041 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8101 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8161 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8221 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8281 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8341 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8401 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8461 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8521 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8581 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8641 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8701 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8761 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8821 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8881 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8941 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     9001 tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc
     9061 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     9121 agcaaaattt aagctacaac aaggcaaggc ttgaccgac
//
