LOCUS       453_pIRES2_short        6666 bp    DNA        circular     02-SEP-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    215_pIRES2_Mouse melanopsin long_IRES_Turbo.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 18, 2017 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
COMMENT     CM in pIRES2:  Melanopsin part of the chimera(CMEO)  , called CM,
            was digested from CM parent with EcoRI and was ligated to EcoRI
            linearized pIRES2 .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        10..589
                     /vntifkey="30"
                     /note="CMV promoter (imm early)"
                     /locus_tag="CMV\promoter\(imm\early)"
                     /label="CMV\promoter\(imm\early)"
                     /ApEinfo_label="CMV\promoter\(imm\early)"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        65..352
                     /vntifkey="30"
                     /note="CAG enhancer"
                     /locus_tag="CAG\enhancer"
                     /label="CAG\enhancer"
                     /ApEinfo_label="CAG\enhancer"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     prim_transcript 2036..2620
                     /vntifkey="91"
                     /note="IRES"
                     /locus_tag="IRES"
                     /label="IRES"
                     /ApEinfo_label="IRES"
                     /ApEinfo_fwdcolor="#e55aec"
                     /ApEinfo_revcolor="#ffe34b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      3714..4020
                     /vntifkey="33"
                     /note="F1 ori"
                     /locus_tag="Rep_Origin_1"
                     /label="Rep_Origin_1"
                     /ApEinfo_label="Rep_Origin_1"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2624..3331
                     /note="Turbo"
                     /locus_tag="Turbo"
                     /label="Turbo"
                     /ApEinfo_label="Turbo"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1442..1468
                     /locus_tag="IL3 mGluR6"
                     /label="IL3 mGluR6"
                     /ApEinfo_label="IL3 mGluR6"
                     /ApEinfo_fwdcolor="#6fdef5"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1844..1939
                     /locus_tag="CT mGluR6"
                     /label="CT mGluR6"
                     /ApEinfo_label="CT mGluR6"
                     /ApEinfo_fwdcolor="#66cdf5"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1940..1999
                     /locus_tag="TS"
                     /label="TS"
                     /ApEinfo_label="TS"
                     /ApEinfo_fwdcolor="#ff120c"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2000..2026
                     /locus_tag="1D4"
                     /label="1D4"
                     /ApEinfo_label="1D4"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    641..1441
                     /locus_tag="hOPN4"
                     /label="hOPN4"
                     /ApEinfo_label="hOPN4"
                     /ApEinfo_fwdcolor="#fffd73"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1469..1843
                     /locus_tag="hOPN4(1)"
                     /label="hOPN4(1)"
                     /ApEinfo_label="hOPN4"
                     /ApEinfo_fwdcolor="#efff77"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgaaccctc cttcggggcc
      661 aagagtcccg cccagcccaa cccaagagcc cagctgcatg gccaccccag caccacccag
      721 ctggtgggac agctcccaga gcagcatctc cagcctgggc cggcttccat ccatcagtcc
      781 cacagcacct gggacttggg ctgctgcctg ggtccccctc cccacggttg atgttccaga
      841 ccatgcccac tataccctgg gcacagtgat cttgctggtg ggactcacgg ggatgctggg
      901 caacctgacg gtcatctata ccttctgcag gagcagaagc ctccggacac ctgccaacat
      961 gttcattatc aacctcgcgg tcagcgactt cctcatgtcc ttcacccagg cccctgtctt
     1021 cttcaccagt agcctctata agcagtggct ctttggggag acaggctgcg agttctatgc
     1081 cttctgtgga gctctctttg gcatttcctc catgatcacc ctgacggcca tcgccctgga
     1141 ccgctacctg gtaatcacac gcccgctggc cacctttggt gtggcgtcca agaggcgtgc
     1201 ggcatttgtc ctgctgggcg tttggctcta tgccctggcc tggagtctgc cacccttctt
     1261 cggctggagc gcctacgtgc ccgaggggtt gctgacatcc tgctcctggg actacatgag
     1321 cttcacgccg gccgtgcgtg cctacaccat gcttctctgc tgcttcgtgt tcttcctccc
     1381 tctgcttatc atcatctact gctacatctt catcttcagg gccatccggg agacaggacg
     1441 gggcgtgccc gagaccttca acgaggccaa gggcaatggc gagtccctgt ggcagcggca
     1501 gcggctgcag agcgagtgca agatggccaa gatcatgctg ctggtcatcc tcctcttcgt
     1561 gctctcctgg gctccctatt ccgctgtggc cctggtggcc tttgctgggt acgcacacgt
     1621 cctgacaccc tacatgagct cggtgccagc cgtcatcgcc aaggcctctg caatccacaa
     1681 ccccatcatt tacgccatca cccaccccaa gtacagggtg gccattgccc agcacctgcc
     1741 ctgcctgggg gtgctgctgg gtgtatcacg ccggcacagt cgcccctacc ccagctaccg
     1801 ctccacccac cgctccacgc tgaccagcca cacctccaac ctccatccag agcagaatgt
     1861 gcagaagcga aagcggagcc tcaaggccac ctccacggtg gcagccccac ccaagggcga
     1921 ggatgcagag gcccacaaga agagcaggat caccagcgag ggcgagtaca tccccctgga
     1981 ccagatcgac atcaacgtga cagagaccag ccaagtggcg cctgcctaag gatccgcccc
     2041 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     2101 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     2161 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     2221 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     2281 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     2341 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     2401 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     2461 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     2521 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     2581 gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtgg gtgaggatag
     2641 cgtgctgatc accgagaaca tgcacatgaa actgtacatg gagggcaccg tgaacgacca
     2701 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgaa
     2761 gatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
     2821 catgtacggc agcaaaacct ttatcaacca cacccagggc atccccgact tctttaagca
     2881 gtccttccct gagggcttca catgggagag gatcaccaca tacgaagacg ggggcgtgct
     2941 gaccgctacc caggacacca gcctccagaa cggctgcctc atctacaacg tcaagatcaa
     3001 cggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     3061 cagcaccgag atgctgtacc ccgctgacag cggcctgaga ggccatagcc agatggccct
     3121 gaagctcgtg ggcgggggct acctgcactg ctccctcaag accacataca gatccaagaa
     3181 acccgctaag aacctcaaga tgcccggctt ctacttcgtg gacaggagac tggaaagaat
     3241 caaggaggcc gacaaagaga cctacgtcga gcagcacgag atggctgtgg ccaggtactg
     3301 cgacctgcct agcaaactgg ggcacagctg aagcggccgc gactctagat cataatcagc
     3361 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3421 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3481 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3541 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat
     3601 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3661 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3721 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3781 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3841 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3901 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3961 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4021 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4081 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4141 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     4201 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4261 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4321 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4381 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4441 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4501 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     4561 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4621 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4681 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4741 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4801 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4861 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4921 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4981 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5041 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     5101 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5161 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5221 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5281 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5341 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5401 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5461 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5521 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     5581 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     5641 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     5701 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     5761 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     5821 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5881 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6661 atgcat     
//
