LOCUS       pJH2523                 7687 bp    DNA        circular     30-AUG-2022
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT      
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      6492..7174
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(1400..1574,2847..2902)
                     /note="calcium indicator; contains permutated EGFP"
                     /codon_start=1
                     /transl_table=11
                     /product="GCaMP3"
                     /protein_id="ADJ53338.1"
                     /db_xref="GI:299818413"
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWN
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGD
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFR
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
                     "
                     /locus_tag="GCaMP3"
                     /label="GCaMP3"
                     /ApEinfo_label="GCaMP3"
                     /ApEinfo_fwdcolor="#ffb7b4"
                     /ApEinfo_revcolor="#ffb7b4"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1969..2403
                     /locus_tag="GFP 1-144"
                     /label="GFP 1-144"
                     /ApEinfo_label="GFP 1-144"
                     /ApEinfo_fwdcolor="#64ff00"
                     /ApEinfo_revcolor="#64ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3223..3224
                     /note="SL1 trans-splice leader [TEC-RED]; see
                     ce1012803ch0303"
                     /locus_tag="SL1 trans-splice leader [TEC-RED]; see
                     ce1012803ch"
                     /label="SL1 trans-splice leader [TEC-RED]; see
                     ce1012803ch"
                     /ApEinfo_label="SL1 trans-splice leader [TEC-RED]; see
                     ce1012803ch"
                     /ApEinfo_fwdcolor="#a7ff00"
                     /ApEinfo_revcolor="#a7ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    1511..1573
                     /locus_tag="M13"
                     /label="M13"
                     /ApEinfo_label="M13"
                     /ApEinfo_fwdcolor="#ff65ca"
                     /ApEinfo_revcolor="#ff65ca"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          2943..2993
                     /locus_tag="intron"
                     /label="intron"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1630..1899
                     /locus_tag="GFP 149-238(end)"
                     /label="GFP 149-238(end)"
                     /ApEinfo_label="GFP 149-238(end)"
                     /ApEinfo_fwdcolor="#64ff00"
                     /ApEinfo_revcolor="#64ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3223..3224
                     /note="SL2 trans-splice acceptor; see yk742h8.5"
                     /locus_tag="SL2 trans-splice acceptor; see yk742h8.5"
                     /label="SL2 trans-splice acceptor; see yk742h8.5"
                     /ApEinfo_label="SL2 trans-splice acceptor; see yk742h8.5"
                     /ApEinfo_fwdcolor="#a7ff00"
                     /ApEinfo_revcolor="#a7ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          4155..4205
                     /locus_tag="intron(1)"
                     /label="intron(1)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_signal     1475..1489
                     /locus_tag="EK recognition"
                     /label="EK recognition"
                     /ApEinfo_label="EK recognition"
                     /ApEinfo_fwdcolor="#0004ff"
                     /ApEinfo_revcolor="#0004ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     7542..7562
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    7514..7536
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="cornflower blue"
                     /ApEinfo_revcolor="cornflower blue"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     variation       1593..1594
                     /locus_tag="del tat->tt"
                     /label="del tat->tt"
                     /ApEinfo_label="del tat->tt"
                     /ApEinfo_fwdcolor="#ffeb14"
                     /ApEinfo_revcolor="#ffeb14"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     variation       3768..3768
                     /locus_tag="P139S(c to t sequenced data pJH1817OZM2281)"
                     /label="P139S(c to t sequenced data pJH1817OZM2281)"
                     /ApEinfo_label="P139S(c to t sequenced data
                     pJH1817OZM2281)"
                     /ApEinfo_fwdcolor="#ffeb14"
                     /ApEinfo_revcolor="#ffeb14"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             5735..6394
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     UTR             4333..5067
                     /locus_tag="unc-54 3UTR"
                     /label="unc-54 3UTR"
                     /ApEinfo_label="unc-54 3UTR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          4282..4332
                     /locus_tag="intron(2)"
                     /label="intron(2)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        27..1305
                     /locus_tag="punc-25"
                     /label="punc-25"
                     /ApEinfo_label="punc-25"
                     /ApEinfo_fwdcolor="#41ffb2"
                     /ApEinfo_revcolor="#41ffb2"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2410..2904
                     /locus_tag="CaM domain"
                     /label="CaM domain"
                     /ApEinfo_label="CaM domain"
                     /ApEinfo_fwdcolor="#ff9d00"
                     /ApEinfo_revcolor="#ff9d00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            1304..1305
                     /gene="unc-25"
                     /locus_tag="unc-25"
                     /label="unc-25"
                     /ApEinfo_label="unc-25"
                     /ApEinfo_fwdcolor="#817c7c"
                     /ApEinfo_revcolor="#817c7c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     exon            4816..4970
                     /locus_tag="aex-5 exon1"
                     /label="aex-5 exon1"
                     /ApEinfo_label="aex-5 exon1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1409..1428
                     /locus_tag="His6"
                     /label="His6"
                     /ApEinfo_label="His6"
                     /ApEinfo_fwdcolor="#c90700"
                     /ApEinfo_revcolor="#c90700"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            1304..1305
                     /gene="unc-25"
                     /locus_tag="unc-25(1)"
                     /label="unc-25(1)"
                     /ApEinfo_label="unc-25"
                     /ApEinfo_fwdcolor="#817c7c"
                     /ApEinfo_revcolor="#817c7c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(1400..1579,1630..1912,1964..2425,2477..2901)
                     /locus_tag="G-CaMP3i"
                     /label="G-CaMP3i"
                     /ApEinfo_label="G-CaMP3i"
                     /ApEinfo_fwdcolor="#00ff13"
                     /ApEinfo_revcolor="#00ff13"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1304..1305
                     /gene="unc-25"
                     /standard_name="Y37D8A.23b"
                     /note="C. elegans UNC-25 protein; contains similarity to
                     Pfam domain PF00282 Pyridoxal-dependent decarboxylase
                     conserved domain contains similarity to Interpro domains
                     IPR002129 (Pyridoxal phosphate-dependent decarboxylase),
                     IPR015421 (Pyridoxal phosphate-dependent transferase,
                     major region, subdomain 1)"
                     /codon_start=1
                     /product="C. elegans protein Y37D8A.23b, confirmed by
                     transcript evidence"
                     /protein_id="CAD45605.1"
                     /db_xref="GI:22859112"
                     /db_xref="GOA:Q8I4E0"
                     /db_xref="InterPro:IPR002129"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015424"
                     /db_xref="UniProtKB/TrEMBL:Q8I4E0"
                     /db_xref="WormBase:WBGene00006762"
                     /translation="MSSAAADESDAVLENLIAKEILPQTGNWEGTEEFLNRIVQVLLK
                     YIKDQNDRDQKILEFHHPDKMQMLMDLSIPEKPESLLKLVKSCEDVLRLGVRTGHPRF
                     FNQISCGLDLVSMAGEWLTATANTNMFTYEIAPVFILMEKSVMARMWEAVGWDPEKAD
                     GIFAPGGAIANLYAMNAARHQLWPRSKHLGMKDIPTLCCFTSEDSHYSIKSASAVLGI
                     GADYCFNIPTDKNGKMIPEALEAKIIECKKEGLTPFFACCTAGSTVYGAFDPLERVAN
                     ICERHKLWFHVDAAWGGGMLLSPEHRYKLAGIERANSVTWNPHKLMGALLQCSACLFR
                     QDGLLFQCNQMSADYLFQQDKPYDVSFDTGDKAIQCGRHNDVFKLWLMWKSKGMEGYR
                     QQINKLMDLANYFTRRIKETEGFELIIENVSFARIPEHLFLVRAFKDSKLGAC"
                     /locus_tag="C. elegans protein Y37D8A.23b, confirmed by
                     transc"
                     /label="C. elegans protein Y37D8A.23b, confirmed by
                     transc"
                     /ApEinfo_label="C. elegans protein Y37D8A.23b, confirmed
                     by transc"
                     /ApEinfo_fwdcolor="#ff9d00"
                     /ApEinfo_revcolor="#ff9d00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          1324..1365
                     /locus_tag="intron(3)"
                     /label="intron(3)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1304..1305
                     /gene="unc-25"
                     /standard_name="Y37D8A.23a"
                     /note="C. elegans UNC-25 protein; contains similarity to
                     Pfam domain PF00282 Pyridoxal-dependent decarboxylase
                     conserved domain contains similarity to Interpro domains
                     IPR002129 (Pyridoxal phosphate-dependent decarboxylase),
                     IPR015421 (Pyridoxal phosphate-dependent transferase,
                     major region, subdomain 1)"
                     /codon_start=1
                     /product="C. elegans protein Y37D8A.23a, confirmed by
                     transcript evidence"
                     /protein_id="CAA21537.1"
                     /db_xref="GI:3925209"
                     /db_xref="GOA:Q9XTP4"
                     /db_xref="InterPro:IPR002129"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015424"
                     /db_xref="UniProtKB/TrEMBL:Q9XTP4"
                     /db_xref="WormBase:WBGene00006762"
                     /translation="MSSAAADESDAVLENLIAKEILPQTGNWEGTEEFLNRIVQVLLK
                     YIKDQNDRDQKILEFHHPDKMQMLMDLSIPEKPESLLKLVKSCEDVLRLGVRTGHPRF
                     FNQISCGLDLVSMAGEWLTATANTNMFTYEIAPVFILMEKSVMARMWEAVGWDPEKAD
                     GIFAPGGAIANLYAMNAARHQLWPRSKHLGMKDIPTLCCFTSEDSHYSIKSASAVLGI
                     GADYCFNIPTDKNGKMIPEALEAKIIECKKEGLTPFFACCTAGSTVYGAFDPLERVAN
                     ICERHKLWFHVDAAWGGGMLLSPEHRYKLAGIERANSVTWNPHKLMGALLQCSACLFR
                     QDGLLFQCNQMSADYLFQQDKPYDVSFDTGDKAIQCGRHNDVFKLWLMWKSKGMEGYR
                     QQINKLMDLANYFTRRIKETEGFELIIENPEFLNICFWYVPSKIRNLEPAEMRARLEK
                     IAPKIKAGMMQRGTTMVGYQPDKQRPNFFRMIISNQAITREDLDFLIKEIVDIGESLE
                     "
                     /locus_tag="C. elegans protein Y37D8A.23a, confirmed by
                     transc"
                     /label="C. elegans protein Y37D8A.23a, confirmed by
                     transc"
                     /ApEinfo_label="C. elegans protein Y37D8A.23a, confirmed
                     by transc"
                     /ApEinfo_fwdcolor="#ff9d00"
                     /ApEinfo_revcolor="#ff9d00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(3252..3407,3459..3652,3704..3767,3769..3896,
                     3948..4115)
                     /locus_tag="worm mCherry"
                     /label="worm mCherry"
                     /ApEinfo_label="worm mCherry"
                     /ApEinfo_fwdcolor="#ff0062"
                     /ApEinfo_revcolor="#ff0062"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     precursor_RNA   2999..3243
                     /locus_tag="gpd-2-gpd-3 intercistronic region"
                     /label="gpd-2-gpd-3 intercistronic region"
                     /ApEinfo_label="gpd-2-gpd-3 intercistronic region"
                     /ApEinfo_fwdcolor="#d7d7c7"
                     /ApEinfo_revcolor="#d7d7c7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 AAGCTTGGGC TGCAgcatgc ggccgcgaaa tatcgatttt ttccgagata tctaaaattg
       61 aaagaaattt tcgattttct gagaaaaata cgaaaaaatc gaaaaaaaaa cccaaatttc
      121 ggttttttcg taaaaaaagt cgtaaaaatg taattttttt cctgaaaaat cggaattttt
      181 tacaaaatat cggaaaatac tcaaaaaaaa gctgaaaatt tcgattttcc ggagaaaaaa
      241 tctttttaaa aaaatatttt ttttttcaga aaataagaaa agccgaaatt tataactatt
      301 tctccggaaa ttcgaaattt ttaacgaaat atcggaataa tttttagatt tttcaagttt
      361 gactttgcga gaaaaaaatc ggaaaaacct cgattcgacg ccgaaaaatg ctccttttcg
      421 aaaagatttt tgaaatttca gaaaatcgac atgcaagcgc gctccacggc gaaatgacaa
      481 cgatgatcca ccgccctcaa aaagttgggt ctcgttaggt atttggcggt aaaactggta
      541 aaactccagt tttgcctcca acgagaccca attttttggg gcggtggtgg agcgcgcttg
      601 cacaagctga aagcattttt ctgcgactcg ataatatttt gaaaacctgt gtcaattctc
      661 gaaattcttt ttttaaaaaa taatcccgag cttctctcag tcctcctcta tgaggatgtt
      721 cctttttttt ggtttttcaa ttttttttaa aattccaaat ttctgttgtg caattcactt
      781 ccccccaaga aatcccaaaa atccccagtt ttccccaaaa atgttccgtt ttcatgtgat
      841 ttttccccca tttttaaaac atttttttga ctttttttta aaatgattat tattattgtt
      901 ttctatttca tggccggtaa attatttttt ttctttcttt ttttttgctc ttttttttca
      961 agaattttcg aattgtttga agggctgctc atctaatctt ttgtcatttt gttctgatgc
     1021 catcatttct gagaggacct ttgaagactc gtcacgaaac gggagggggg ctcaagtgag
     1081 cattattatt attattattg tcgcaaaaag tttaccccgg gctccccctg gctcccctct
     1141 ttgagcaagg gtttaagggc tcattttgat gacgaattgc tcattgggat tatagtcacg
     1201 cccctctttt ggagcaacta cacaactgag ccacagtaat ccttgggggc ggggtcagta
     1261 ggaccccctc cggaataggg aaaagctcag ttcaccgcca aaaattggCC AAAGGACCCA
     1321 AAGgtatgtt tcgaatgata ctaacataac atagaacatt ttcagGAGGA CCCTTGGCTA
     1381 GCGTCGACGg atccaaaaaA TGGGTTCTCA TCATCATCAT CATCATGGTA TGGCTAGCAT
     1441 GACTGGTGGA CAGCAAATGG GTCGGGATCT GTACGACGAT GACGATAAGG ATCTCGCCAC
     1501 CATGGTCGAC TCATCACGTC GTAAGTGGAA TAAGACAGGT CACGCAGTCA GAGCTATAGG
     1561 TCGGCTGAGC TCACTCGAGg taagtttaaa cattatatac taactaacgg attatattta
     1621 aattttcagA ACGTCTATAT CAAGGCCGAC AAGCAGAAGA ACGGCATCAA GGCGAACTTC
     1681 AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCTACCACTA CCAGCAGAAC
     1741 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CGTGCAGTCC
     1801 AAACTTTCGA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
     1861 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG GCGGTACCGG AGgtaagttt
     1921 aaacagttcg gtactaacta accctgatta tttaaatttt cagGGAGCAT GGTGAGCAAG
     1981 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
     2041 GGCCACAAGT TCAGCGTGTC CGGCGAGGGT GAGGGCGATG CCACCTACGG CAAGCTGACC
     2101 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
     2161 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
     2221 TTCAAGTCCG CCATGCCCGA AGGCTACATC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
     2281 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
     2341 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
     2401 AACACGCGTG ACCAACTGAC TGAAGgtaag tttaaacttg gacttactaa ctaaccatac
     2461 atatttaaat tttcagAGCA GATCGCAGAA TTTAAAGAGG CTTTCTCCCT ATTTGACAAG
     2521 GACGGGGATG GGACAATAAC AACCAAGGAG CTGGGGACGG TGATGCGGTC TCTGGGGCAG
     2581 AACCCCACAG AAGCAGAGCT GCAGGACATG ATCAATGAAG TAGATGCCGA CGGTGACGGC
     2641 ACAATCGACT TCCCTGAGTT CCTGACAATG ATGGCAAGAA AAATGAAAGA CACAGACAGT
     2701 GAAGAAGAAA TTAGAGAAGC GTTCCGTGTG TTTGATAAGG ATGGCAATGG CTACATCAGT
     2761 GCAGCAGAGC TTCGCCACGT GATGACAAAC CTTGGAGAGA AGTTAACAGA TGAAGAGGTT
     2821 GATGAAATGA TCAGGGAAGC AGACATCGAT GGGGATGGTC AGGTAAACTA CGAAGAGTTT
     2881 GTACAAATGA TGACAGCGAA GTAggatcCC CGAGATCTTC AGCGCTCAGC ATTCGTAGAA
     2941 TTgtaagttt aaactttctc atactaacta acgcgctcta tttaaatttt cagAATTCgc
     3001 tgtctcatcc tactttcacc tagttaactg cttgtcttaa aatctatgct tctctttagt
     3061 atctaaaatt ttcctagaag cttacaagta tataaatggt ctcttctcaa taaaggttgt
     3121 atatttattc atcttattga atctgccatt tcctcgtttt tgcgagttta tataccttcc
     3181 aattttcttt ctattgtatt ttcaacttct aattttaatt cagggaaact gcttcaacgc
     3241 atcGGTACCC tATGGTCTCA AAGGGTGAAG AAGATAACAT GGCAATTATT AAAGAGTTTA
     3301 TGCGTTTCAA GGTGCATATG GAGGGATCTG TCAATGGGCA TGAGTTTGAA ATTGAAGGTG
     3361 AAGGAGAAGG CCGACCATAT GAGGGAACAC AAACCGCAAA ACTAAAGgta agtttaaaca
     3421 tatatatact aactaaccct gattatttaa attttcagGT AACTAAAGGC GGACCATTAC
     3481 CATTCGCCTG GGACATCCTC TCTCCACAGT TCATGTATGG AAGTAAAGCT TATGTTAAAC
     3541 ATCCGGCAGA TATACCAGAT TATTTGAAAC TTTCATTCCC GGAGGGTTTT AAGTGGGAAC
     3601 GCGTAATGAA TTTTGAAGAC GGAGGAGTTG TTACAGTGAC GCAAGACTCA AGGTAAGTTT
     3661 AAACAGTTCG GTACTAACTA ACCATACATA TTTAAATTTT CAGCCTCCAA GATGGAGAAT
     3721 TTATTTATAA AGTCAAACTT CGAGGAACGA ATTTCCCCTC GGATGGAtCT GTTATGCAGA
     3781 AGAAGACTAT GGGATGGGAA GCTTCAAGTG AAAGAATGTA CCCTGAAGAC GGTGCTCTTA
     3841 AGGGAGAGAT TAAACAACGT CTTAAATTGA AAGATGGAGG ACATTACGAT GCTGAGgtaa
     3901 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagGTG AAGACAACTT
     3961 ACAAAGCCAA AAAACCAGTT CAGCTGCCAG GAGCGTACAA TGTTAATATT AAACTGGATA
     4021 TCACCTCCCA CAACGAGGAT TACACTATCG TTGAGCAATA TGAAAGAGCT GAAGGGCGGC
     4081 ACTCGACAGG TGGCATGGAT GAATTGTATA AGTAGGAGCT CCCGAGATCT TCAGCGCTCA
     4141 GCATTCGTAG AATTgtaagt ttaaactttc tcatactaac taacgcgctc tatttaaatt
     4201 ttcagAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA
     4261 ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa cgagtaatat
     4321 ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA
     4381 TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAAAC
     4441 TTCTTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA TGAAATTGTG
     4501 TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG CTCCCTCCCC
     4561 CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT TCTTATGTTT
     4621 TTTTTACTTC TGATAAATTT TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT
     4681 TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT
     4741 CTCATGACGT CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA
     4801 ATCAAGTGAA AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGG TTTCCCCTAT
     4861 TGTTTGTCAA GAGTTTCGAG GACGGCGTTT TTCTTGCTAA AATCACAAGT ATTGATGAGC
     4921 ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG GTGAGTAGAA
     4981 GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT GCCTTAAATG ACAGAATACA
     5041 TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC CGTACGGGCC CTTTCGTCTC
     5101 GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA
     5161 GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT
     5221 GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC
     5281 CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT
     5341 TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA
     5401 GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA
     5461 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA
     5521 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC
     5581 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA
     5641 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT
     5701 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG
     5761 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA
     5821 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT
     5881 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT
     5941 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA
     6001 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA
     6061 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA
     6121 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC
     6181 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC
     6241 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG
     6301 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT
     6361 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA
     6421 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT
     6481 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA
     6541 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG
     6601 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC
     6661 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT
     6721 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC
     6781 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
     6841 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG
     6901 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA
     6961 TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
     7021 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG
     7081 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
     7141 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
     7201 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC
     7261 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT
     7321 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT
     7381 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC
     7441 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
     7501 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA
     7561 TGATTACGCC AAGCTgtaag tttaaacatg atcttactaa ctaactattc tcatttaaat
     7621 tttcagAGCT TAAAAATGGC TGAAATCACT CACAACGATG GATACGCTAA CAACTTGGAA
     7681 ATGAAAT     
//
