LOCUS       pHAGE-IRES-puro-       12979 bp DNA     circular SYN 29-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12979)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12979
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #a6acb3"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..7254
                     /codon_start=1
                     /label=MisCas13b
                     /note="color: #993366"
                     /translation="MFVRYSPESWPRHFHGLQQRLPTPIGQHTFLSDNFNTMDIRLSKN
                     RALLAAYLNMAQQNVYVTLLHISQLMGVEETKDLSNRMYDFQILQELKTGKKGAESEQM
                     VKSERVQKIILLLYRNFPFLKAFVEAKYKQQEKNNGGKNKGGGKNTQKNKKTPDNTSNE
                     EQLEFTESELGKLPIPPHEYRTILCDVFRLINDLANEYTAFSPEKRTGGAKESHEKCQK
                     KMLSYLYLCLDGGRRVVKERFKDTFQEKDFNFLTQGPRYSLQKDGDKKKAEERPNFYYR
                     LKDDQGLFSYVGILLFISLFLHKRYISEMLAQSGLFYTPRYWKGQPVNVELERRIISEI
                     FSIYRIRLPKERIDSTRPDYALGLDMLNELQKCPNELYETFSPKDQAEFRVKREYDESG
                     EGTEEGEVLLKRFEDRFPYFAIRYLDENRVFDDIRFQVSIGKYRYKFYNKLCIDVVSSN
                     RNDDCYRLRSLQKEINGFGRLNEVEYERILRYGSHIHVEKPEGDDTPYLTDHHTSYLFN
                     GNRIGLLFNQENGRHDLEEIRGNCLYLPHLTFASSSEGKDARRVPPIAWLSTYELPALI
                     FHHLLCRHYGLERTATESIIKECVGLYHRLFTDIRDGKLLPGEALGIQAEESTRSEISR
                     IKKGEPLPQNISEKIETFLSTHYCTEQVCLHIQDIPKKIIDYLIGKNIDQDMSGLFKQR
                     SRERINKMYESTKIRLKKFKTDLDLQGNGDNKFGKKRYTDIRPGQLADYLAKDLLFFQP
                     YDDEKKNKVTGLNYQILQAALAIYNHNLDELRRLFKNARLLVPENPKMEHPFLGSVLHK
                     SPRTTLQFYAYYLLEKISYLKKRLDDPNETFADLFRYKQRQKWQTRDSQWYRALAGRYM
                     AEMMEEGTMNFKPVELPRGLFERPIKRLLTNILTKRTDSDSQALLKIVESPRCNVSYLI
                     AQYFTLILHDDSQPFYHPNKRYKRTYQYFNILNNRKERNKLVQVYYTLDEMEAYFKDTE
                     SLKKRYKDYLTSSEAKKHPAPEQRLKKYLSEYRKNETTIRRYRVQDMLVFLMAKILLLE
                     KGGLEAGELEQYKLSHIQPTTGQNSGQNILSLQVPFNITVKLDDGTERSITQEVIKVKN
                     YGDFFRFLYDDRVASLLPYTQEDIPLERALLEKELEGYDRERIPIFKSVHCMEKEILRL
                     CPELNDPQSSQYKYTTDIGTEKIVKENFRAMLTELCSRGLLPENEIEMMVEIANSFSAN
                     RYACVNLKGKNIPMIAISIQRKFDGVVGRTIGKNKN"
     misc_feature    3988..4032
                     /label=Feature 18
                     /note="color: #a6acb3"
     primer_bind     4498..4517
                     /label=Primer 1
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(4517..4536)
                     /label=dMis-H1-Pgu-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     4546..4565
                     /label=Primer 2
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    5092..5133
                     /label=Feature 19
                     /note="color: #a6acb3"
     misc_feature    5332..5451
                     /label=Feature 20
                     /note="color: #a6acb3"
     primer_bind     complement(5339..5350)
                     /label=Primer 2
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6695..6714)
                     /label=Primer 3
                     /note="color: #a020f0; direction: LEFT"
     CDS             7282..7998
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             8026..8073
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8092..8157
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8164..8731
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             8751..9350
                     /codon_start=1
                     /label=PuroR
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9369..9957
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #a6acb3"
     CDS             complement(9840..9851)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     LTR             10032..10265
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        10470..10574
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             10575..11435
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11606..12194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    12605..12626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        12641..12671
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
     protein_bind    12679..12695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     12703..12719
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catgttcgtg
     3481 agatatagcc ctgagtcctg gcctaggcac ttccacggcc tgcagcagag actgcccacc
     3541 cccatcggcc agcacacctt cctgagtgat aactttaaca ccatggacat ccggctgagt
     3601 aagaatagag ccctgctggc agcttacctg aatatggccc agcagaacgt gtacgtgacc
     3661 ctgctgcaca tctcccagct gatgggagtg gaggagacca aagacctgtc taaccgcatg
     3721 tacgactttc agattctgca ggagcttaag accgggaaga agggcgccga gtctgagcag
     3781 atggtgaagt ctgagagagt gcagaagatc attctcctgc tgtatcgcaa cttccctttc
     3841 ctgaaggcct tcgtggaggc taagtacaag cagcaggaga agaacaatgg gggcaaaaac
     3901 aagggcggcg gaaaaaacac acagaagaat aagaaaaccc ctgacaatac aagcaacgag
     3961 gagcagctgg agttcactga gagtgagctg ggcaagctgc ccattccacc acatgagtac
     4021 agaaccatcc tgtgtgatgt gttcaggctg atcaatgacc tggccaatga gtacacagcc
     4081 ttcagccctg agaaacgcac tggcggcgct aaggagtccc acgaaaagtg ccagaagaag
     4141 atgctgagct acctgtacct gtgcctggac ggaggcagaa gggtggtgaa ggagcggttc
     4201 aaggacacct tccaagagaa ggactttaac tttctcaccc agggcccaag atacagcctc
     4261 cagaaagacg gagataagaa gaaggccgag gaaagaccta atttttacta cagactgaag
     4321 gatgaccagg gactgttctc ttacgtcggt atcctcctgt tcatcagcct gttcctgcac
     4381 aagaggtata tttccgagat gctggcccag tccgggctgt tttatacacc tcggtactgg
     4441 aagggccagc cagtgaacgt ggaactggag aggaggatca tcagtgagat cttttccatc
     4501 tatcgcatca ggctgcccaa ggagagaatc gactccacca gaccagatta cgccctcggc
     4561 ctggatatgc tgaatgagct gcagaagtgc cctaatgagc tgtatgagac cttctcacct
     4621 aaagaccagg ctgagtttag agtgaagcgg gagtatgacg agagcggcga aggcacagaa
     4681 gagggcgagg tgctgctgaa aagattcgag gacagattcc cttatttcgc cattcgctat
     4741 ctggacgaga atagagtgtt cgacgacatc agattccagg tgagcatcgg caagtatagg
     4801 tacaaattct acaataagct gtgcatcgac gtggtgagca gcaatcggaa cgatgactgc
     4861 tatcgcctga ggtccctgca gaaagagatt aatggcttcg gcagactgaa tgaagtggag
     4921 tacgagagaa tcctgagata cgggtctcac atccacgtgg agaagcctga gggcgacgac
     4981 accccctacc tgactgacca tcataccagc tacctgttta acggcaatag gatcggcctg
     5041 ctgttcaacc aggagaacgg caggcacgat ctggaggaga tcagaggaaa ctgcctgtat
     5101 ctgcctcacc tgacttttgc atcctcctcc gagggaaagg atgccaggcg cgtgcccccc
     5161 atcgcctggc tgtccaccta tgagctgcct gccctgatct tccaccacct gctgtgcaga
     5221 cactatggcc tggagagaac tgccaccgag agcatcatca aggaatgtgt gggcctgtac
     5281 caccggctgt ttactgatat ccgcgacggt aaactgctcc ccggagaggc cctgggcatc
     5341 caggccgagg agtccacaag gagcgagatt agtaggatca agaagggtga acccctgccc
     5401 cagaacatct ctgaaaagat tgaaaccttc ctgagcactc actactgcac cgaacaggtg
     5461 tgcctgcaca tccaggacat tcctaagaaa atcatcgatt acctgatcgg gaaaaacatc
     5521 gaccaggaca tgagcggact ctttaagcag agaagcagag agcggattaa taagatgtat
     5581 gagtccacta aaattcgcct caagaagttc aagaccgacc tggacctgca gggcaacggc
     5641 gacaataagt ttggaaagaa gaggtacact gacattaggc caggacagct ggccgactat
     5701 ctggccaagg acctgctgtt cttccagcca tatgacgacg agaagaagaa taaggtcact
     5761 ggactgaact atcagatcct gcaggccgca ctggccatct acaatcacaa cctggatgag
     5821 ctcagacgac tcttcaaaaa tgcccggctg ctcgtgcccg agaatcctaa gatggagcac
     5881 cctttcctgg gctctgtgct gcacaagtcc ccccggacca ctctgcagtt ctacgcctac
     5941 tacctgctcg agaagatctc ttacctgaaa aagagactcg acgatcctaa cgagacattc
     6001 gccgatctgt ttagatataa gcagaggcag aaatggcaga ctagagacag ccagtggtat
     6061 agggccttgg ctggcaggta catggctgag atgatggaag agggcaccat gaattttaaa
     6121 cccgtggagc tgccaagagg gctgtttgaa agacctatca agcggctgct gaccaatatc
     6181 ctgactaagc ggaccgacag cgatagccag gccctgctga agatcgtgga gagccccagg
     6241 tgcaacgtga gctacctgat tgctcagtat ttcactctga ttctgcacga cgattcccag
     6301 cccttttacc accctaacaa acgctacaaa aggacctacc agtatttcaa catcctgaac
     6361 aatagaaagg agcggaataa gctcgtgcag gtgtactaca ctctggatga gatggaggct
     6421 tactttaagg ataccgaatc tctgaagaag aggtataagg actacctgac tagctccgag
     6481 gctaagaagc acccagcccc cgagcagagg ctgaagaagt atctgtcaga gtatcgcaag
     6541 aatgagacca ccattagacg gtacagagtg caggacatgc tggtgtttct gatggccaaa
     6601 attctgctgc tggaaaaggg cggcctcgaa gccggcgagc tggagcagta caagctgtcc
     6661 cacattcagc ccaccaccgg ccagaacagc ggccagaaca tcctgagcct gcaggtgccc
     6721 tttaacatca ccgtgaagct ggacgatggc acagagagaa gcatcaccca ggaagtgatc
     6781 aaggtgaaaa attatggcga cttctttaga tttctctatg atgaccgggt ggcatccctg
     6841 ctgccttata ctcaggagga cattcccctg gagcgggccc tgctggagaa ggagctggag
     6901 ggatacgaca gggagagaat ccctattttc aagagcgtgc actgcatgga gaaggagatc
     6961 ctgaggctgt gccctgaact gaatgatccc cagtccagcc agtacaagta taccacagat
     7021 attggcactg agaagatcgt gaaggagaat ttcagggcca tgctgactga gctgtgtagc
     7081 cggggcctgc tccctgagaa cgagatcgag atgatggtgg agatcgccaa tagcttttcc
     7141 gccaatagat acgcctgcgt gaacctgaag ggcaagaata tcccaatgat cgccatctcc
     7201 atccagcgga agttcgacgg cgtggtgggc agaaccatcg gaaagaataa gaacagtcta
     7261 gaggcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     7321 cccatcctgg tcgagctgga cggagacgta aacggccaca agttcagcgt gtccggcgag
     7381 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     7441 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     7501 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     7561 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     7621 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     7681 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     7741 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     7801 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     7861 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     7921 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     7981 atggacgagc tgtacaagtc gaattctaag ccaggattcc atatgaagcg acctgccgcc
     8041 acaaagaagg ctggacaggc taagaagaag aaacaacgcg tgcagctaag cgactacaaa
     8101 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagtaa
     8161 ggatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     8221 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     8281 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     8341 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     8401 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     8461 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     8521 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     8581 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     8641 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     8701 cgtggttttc ctttgaaaaa cacgatgata aggatccacc ggagcttacc atgaccgagt
     8761 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     8821 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     8881 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     8941 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     9001 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     9061 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     9121 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     9181 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     9241 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     9301 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccatcga
     9361 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     9421 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     9481 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     9541 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     9601 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     9661 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     9721 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     9781 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     9841 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     9901 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     9961 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
    10021 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
    10081 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    10141 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    10201 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    10261 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
    10321 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
    10381 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    10441 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10501 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10561 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10621 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10681 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10741 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10801 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10861 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10921 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10981 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    11041 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    11101 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    11161 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    11221 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    11281 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    11341 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    11401 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    11461 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    11521 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11581 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11641 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11701 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    11761 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11821 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11881 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11941 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    12001 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    12061 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12121 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12181 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    12241 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    12301 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    12361 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    12421 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    12481 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    12541 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    12601 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    12661 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
    12721 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    12781 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    12841 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    12901 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    12961 gtactacaaa cttagtagt
//
