LOCUS       pHAGE-IRES-puro-       12568 bp DNA     circular SYN 18-JUL-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12568
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #a6acb3"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..6843
                     /codon_start=1
                     /label=Hgm4Cas13b
                     /note="color: #993366"
                     /translation="MKIPQIIENNKHLFGAYSTMALSNIRNILDHIATLADIENDFYSD
                     SDSYWYHPCIKIIDPRNNCTDAAKADFVIEKIKSHFPFVPIVAEAQRQSDILRAKKQLD
                     IAVRNRDYQKQEELKHRQKSLLSVTNADIYRVLNNMLWVLANYANQTVAYLISDIKFED
                     GSDFLNYNEQPLSRMLNNYFTIALRDTANKYSYSPEALSFIQSSRYKTENRRKILDTDF
                     FLSIQHNNSDTNPKNLHLSGVGVALLICLFLEKKYVNVFLQKLPIYGPYKKQSMEANII
                     RQSFSIHTVKLPKERIVSEKSDFTIALDMLNELKRCPKQLFSTLSYADQNAFRIVSSDM
                     SDVLQVRHTDRFAQLSLEYIDRRELFSDIRFHLNMGKLRYLKTADKHCIDGVSRVRVLE
                     GKINAFGRIHEFEARRKEQGFVEWHEQAARLIKTNKNIEIRDFEHVKRDDATPDSYPYI
                     IDTYTHYLLENNKIEMRFGDYLPKLVKVDDQKWTVYNEMPTCCMSALELPAMMFHMMLC
                     GSEATEHLIKAEVEKYKKLFGAMANGTLSKENIGNFEIAMEDLPKKVIDCVNRKKSGKS
                     FVKQIKKEIAEMLADTNLRIVRLKSDKRSVASTQNKMGKRGFRSIQPGKLADWLAADIV
                     KHQPSLFSGDDYGTDRITGMNYRVMQSTIATFNAATPEHSLDELKRVFSAAQLIQCQER
                     EHPFLQKALNRNPQNTIDFYEFYLQAKRAYYQSLRQSIENGEDVKLPYLNPDRNKWVRR
                     GASFYSTMGDIYLEDMPIELPRQMFDKEIKKALAQLPSMKGVDMKQSNVTFLIAEYLKR
                     ELKDDFQPFYQWNRNYRFTDMMICEENCDTKALASKFIPLELREEIWEKRGELKDAYKR
                     WAFGRLNKNPDIKRLSPAEKDELLEKRIAKCRNEYQKSEKIIRRYKVQDVLMFMMVKSM
                     FAQGVFTAESKEFALSTITPDAERGILSEVVPIDFKFSIDGKTYTIHSNGMKIKNYGDF
                     YKLINDKRMKSLLNIVTHSTIEKDLLEKEFSTYDDKRPEAVEIIFEFEKAAYEKHPELK
                     ELLTPERKFDFRALLEELQAKSDLSQIDARYLSQIANAFNANAYPRNLKITSNIPEIAQ
                     EMVNIFRITDPYKKGKK"
     primer_bind     complement(4602..4640)
                     /label=Hgm4Cas13b-K386A-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     4610..4656
                     /label=Hgm4Cas13b-K386A-F
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(4630..4668)
                     /label=Hgm4Cas13b-R396A-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     4638..4680
                     /label=Hgm4Cas13b-R396A-F
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(5347..5368)
                     /label=Mhd-R191-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6500..6519)
                     /label=Primer 2
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6701..6720)
                     /label=Primer 1
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6831..6851)
                     /label=Hgm4-SNAPf-R
                     /note="color: #a020f0; direction: LEFT"
     CDS             6871..7587
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7615..7662
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7681..7746
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7753..8320
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             8340..8939
                     /codon_start=1
                     /label=PuroR
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8958..9546
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #a6acb3"
     CDS             complement(9429..9440)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     LTR             9621..9854
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        10059..10163
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             10164..11024
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11195..11783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    12194..12215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        12230..12260
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
     protein_bind    12268..12284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     12292..12308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catgaagatc
     3481 ccccagatca tcgagaacaa caagcacctg ttcggcgcct acagcactat ggccctgagc
     3541 aacattcgca acatcctgga tcacatcgcc accctggctg acatcgaaaa tgacttctac
     3601 agcgatagcg actcttattg gtaccaccca tgcatcaaga tcatcgaccc tcggaacaac
     3661 tgcaccgacg ccgccaaggc tgatttcgtg atcgagaaga ttaagtctca tttcccattc
     3721 gtgccaatcg tggccgaggc ccagaggcag tccgatatcc tgagagccaa gaagcagctg
     3781 gacatcgccg tgagaaatag ggattaccag aagcaggagg agctgaagca ccggcagaag
     3841 tccctgctgt ccgtgaccaa tgccgatatc taccgggtgc tgaataacat gctgtgggtg
     3901 ctggccaact acgccaacca gaccgtggcc tacctgatca gcgacatcaa gttcgaagat
     3961 ggttccgact ttctcaatta taacgagcag cctctgtccc ggatgctgaa taactacttc
     4021 accatcgccc tccgggacac cgctaacaag tacagctata gccctgaggc cctgagcttc
     4081 atccagtcaa gtaggtataa gactgagaat cggcggaaaa tcctggacac agactttttc
     4141 ctgtctatcc agcacaacaa cagcgatact aaccctaaga accttcacct gtccggcgtg
     4201 ggcgtggccc tgctgatttg tctgtttctg gagaaaaaat atgtgaatgt gtttctgcag
     4261 aagctgccca tctacggacc ctacaagaag cagtccatgg aggccaacat catcagacag
     4321 agcttttcca tccacaccgt gaagctgcca aaggagcgga ttgtctctga aaagagcgac
     4381 ttcaccattg cactggatat gctgaacgag ctgaagagat gccccaagca gttgtttagc
     4441 accctgtcct acgctgacca gaacgccttc agaatcgtga gcagtgatat gagcgacgtg
     4501 ctgcaggtga ggcatactga ccggttcgcc cagttaagcc tggagtacat cgatcggcgg
     4561 gaactgttta gcgacattag atttcacctg aacatgggca agctgcggta cctgaaaacc
     4621 gctgacaagc actgcatcga tggtgtgtct agggtgagag tgctggaggg taaaattaac
     4681 gcctttggcc gcattcatga gtttgaggcc aggcggaagg agcagggatt cgtggagtgg
     4741 cacgagcagg cagcccggct gatcaagacc aataagaata tcgaaatcag ggactttgag
     4801 cacgtgaagc gcgacgacgc cacacccgat agctacccct acattattga cacttacacc
     4861 cactatctgc tggagaataa taagatcgag atgaggtttg gcgactacct cccaaaactg
     4921 gtgaaggtgg acgaccagaa gtggaccgtg tacaacgaaa tgcccacatg ttgcatgtcc
     4981 gccctggagc tgcccgccat gatgttccac atgatgctgt gcggcagcga agcaacagag
     5041 catctgatca aggccgaggt ggagaagtat aagaagctct ttggcgccat ggctaacggc
     5101 acactgtcta aggagaatat cggcaacttc gagattgcca tggaggacct gccaaagaaa
     5161 gtgatcgact gcgtgaatag gaagaagtct gggaagtctt ttgtcaagca gattaagaag
     5221 gagatcgccg agatgctggc cgacaccaac ctgaggattg tgagactgaa gtccgataag
     5281 cgcagcgtgg cctccaccca gaataagatg ggcaaacggg gctttagaag cattcagccc
     5341 ggcaagctgg ccgactggct cgccgccgat atcgtgaaac accagcctag cctgtttagc
     5401 ggcgacgact acggtaccga taggattacc ggcatgaatt accgggtcat gcagtctact
     5461 atcgctacct tcaatgctgc cacccccgag cacagcctgg acgagctgaa aagagtgttt
     5521 agcgccgcac agctgattca atgtcaggag agggagcacc cttttctcca gaaggccctg
     5581 aatcgcaatc cccagaacac catcgacttc tacgagttct atctgcaggc taagagagct
     5641 tactatcagt ccctgcggca gtctatcgag aacggcgagg acgtgaaact gccatacctg
     5701 aaccctgatc gcaataagtg ggtgaggaga ggggccagct tttacagcac catgggagat
     5761 atctacctgg aggacatgcc cattgagctg ccaaggcaga tgttcgacaa ggagatcaag
     5821 aaggccctgg cccagctgcc ttccatgaag ggcgtggata tgaagcagag caacgtgacc
     5881 ttcctgatcg ccgagtacct caagagagaa ctgaaggatg actttcagcc attttatcag
     5941 tggaacagaa attacaggtt tacagacatg atgatttgcg aggagaactg cgacaccaag
     6001 gccctggcat ccaaattcat ccctctggag ctccgcgagg agatctggga gaagcggggc
     6061 gagctgaaag atgcctacaa aagatgggct ttcggcagac tgaataagaa tcctgatatc
     6121 aagcggctga gccctgccga gaaggacgaa ctgctggaaa agcgcatcgc caagtgtcgc
     6181 aacgagtacc agaagtccga gaagatcatt cggagataca aggtgcagga tgtcctgatg
     6241 ttcatgatgg tcaagtccat gtttgctcag ggagtgttca ccgccgagtc caaggagttt
     6301 gccctgtcca caatcacccc tgacgccgag cggggcattc tgtccgaagt ggtgcccatc
     6361 gatttcaagt tttccatcga cggaaagacc tacaccatcc actccaacgg catgaagatc
     6421 aagaactacg gcgattttta caagctgatc aacgacaaga gaatgaagtc tctgctgaat
     6481 atcgtgaccc actctactat cgagaaggac ctgctggaga aggagtttag cacctacgac
     6541 gataagagac ctgaggccgt ggagatcatc ttcgagtttg agaaggccgc ctacgagaag
     6601 caccccgagc tgaaggagct gctgactccc gagcgcaagt ttgatttcag ggccctgctg
     6661 gaggagctcc aggccaagtc tgacctgtct cagatcgatg ccagatatct gagccagatc
     6721 gccaacgcct tcaacgccaa cgcctacccc cggaatctga agatcacatc caacatccct
     6781 gagatcgccc aggagatggt gaacatcttc aggatcaccg acccctacaa gaaaggcaag
     6841 aagagtctag aggcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     6901 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     6961 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     7021 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     7081 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     7141 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     7201 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     7261 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     7321 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     7381 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     7441 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     7501 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     7561 actctcggca tggacgagct gtacaagtcg aattctaagc caggattcca tatgaagcga
     7621 cctgccgcca caaagaaggc tggacaggct aagaagaaga aacaacgcgt gcagctaagc
     7681 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
     7741 gacaagtaag gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     7801 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     7861 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     7921 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     7981 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     8041 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     8101 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     8161 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     8221 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     8281 ccacggggac gtggttttcc tttgaaaaac acgatgataa ggatccaccg gagcttacca
     8341 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     8401 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     8461 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     8521 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     8581 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     8641 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     8701 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     8761 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     8821 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     8881 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     8941 gcccatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     9001 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     9061 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     9121 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     9181 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     9241 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     9301 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     9361 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     9421 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     9481 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     9541 ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     9601 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     9661 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9721 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9781 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9841 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     9901 gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt
     9961 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
    10021 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    10081 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    10141 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    10201 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    10261 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    10321 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    10381 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
    10441 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    10501 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    10561 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    10621 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    10681 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    10741 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    10801 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    10861 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    10921 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    10981 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    11041 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    11101 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    11161 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    11221 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    11281 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    11341 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    11401 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    11461 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    11521 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    11581 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    11641 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    11701 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    11761 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    11821 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    11881 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    11941 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    12001 ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc
    12061 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    12121 aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg
    12181 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    12241 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    12301 gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    12361 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    12421 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    12481 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    12541 gttaaatatg tactacaaac ttagtagt
//
