LOCUS       pHAGE-IRES-puro-       12997 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12997
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #a6acb3"
     primer_bind     2088..2112
                     /label=U6.PLL3.7
                     /note="color: #a020f0; direction: RIGHT"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     3280..3300
                     /label=EF1a-F:
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     3353..3373
                     /label=EF-a£¨PEF-F)
                     /note="color: #a020f0; direction: RIGHT"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..7272
                     /codon_start=1
                     /label=dPba3Cas13b
                     /note="color: #993366"
                     /translation="MAFDKPRPKRRQARSDFYITDKAIMGGYFNLAQLNFFKTLMEIFT
                     KAGIDVSKIKQDNSPKYLMILIKKLTHDDEKIDDKKWADALDLSNECLLKLQQLLYKHF
                     PFLGPVMGGEASYNIYKLKDGHPEVKVANDVMRGMKLEDCLAVLRHFALCLNDCANFYT
                     AFNPYNSIDAQKEQYLSQNKVAVWLEKVKDASRRIVKQNNQLTSQDMEFLTGIDHMKPQ
                     DKVDDFGNIMTDKKGYTIKEYVEYEDYYFRIRGKRYLVDAAGAKLADQEPRNALSDFGV
                     VFLCTLFLENDQSRRMLDELRLFETGPYDGSRDGDEFKNDILREILLFYRARIPRGKRL
                     DPMDDTTLLAMDMLNELRKCPMPLYDVLSREGQRFFEDEVKHPNDLTPEVAKRLRSSDR
                     FPYLALRYIDLNKCFDNIRFQVQLGKFRYKFYDKTTIDGEQVVRGLQKEVNAYGRFQDV
                     EHYRQEKYADMLQQTELVETGEEDITIANFIPDTPQSSPYLSDRTASYNIHNNRIGLLW
                     NMPGEHEVLTGDEKMYLPDLNVDDNGKADVFLPAPKASLSVRDLPALVFYLYLQNQHPD
                     LTPAEAIIQQKYNALVRFFEDVSTGRLLPVKGIAELEAAIDKYGHLTVHEIPEKLRDYL
                     AGVAGTEDDDDCADRLDCYAMGILEKRYRQVAARIDQLKEARKMVGDKMNRYGKKSYID
                     VRPRRLAEQLAQSIVEWQPATDNGRHKMTGKNFQRLIAFLARYIDEVPLQELKDLMTRA
                     RLLEGPVAHPFLQQVLDKNPLNIEVLYDLYFKEEKRYLDKYIDIEINKQGEEVYWLKAD
                     ADLSKIPFLNSSRLRFHKRDSASFQRLAARYLQVDGRQSTILLPDGLFTPHILSALKST
                     LYQENHDLQDHLQDQELNQNASYLMSVYLEHVLHDSAQPFYLSNSNDSPFARRYDLFAI
                     VQNKKRGAELLPGPLTPVEINRRLTAKTPDGSAKLIERDIVALLEDMARKTEQRIHKLP
                     QDLYLKKKKQAQEERIKLQRRLKHKITDVKKNERAIRRYKTQDMAIFLMAQQMLANQLS
                     WQNQEGEVPLKLANVCNDGFLSQTMKVSFPYEVPLKEDKTKKVTIDIVQDNMSLKNYGL
                     LYKLYHDERVVTLLQNIVDQQQISFAELTAELSLYDQNRSKAFSNFQQFEKLAFETNRE
                     ALSNPEHRGFFLEKVNKKNQVIRYERRNNFDALLDLLDAIEAHQLDPRDRQWLTSVANA
                     FCANSYYKIDMQQIEKKLPTIIQQIVEIVQRITDKASHPSNR"
     primer_bind     complement(4406..4436)
                     /label=dBba-Pba3-R1
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4406..4425)
                     /label=dPba3-Bba2-R1
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4478..4497)
                     /label=Pba3-H1-Pgu-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     6705..6725
                     /label=dPba3-Bba-F2
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     6706..6725
                     /label=dPba3-Bba2-F2
                     /note="color: #a020f0; direction: RIGHT"
     CDS             7300..8016
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7345..7366)
                     /label=PEGFP-N-3(EGFP-N)
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     7953..7974
                     /label=PEGFP-C-5(EGFP-C)
                     /note="color: #a020f0; direction: RIGHT"
     CDS             8044..8091
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8110..8175
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8182..8749
                     /label=IRES
                     /note="color: #a6acb3"
     primer_bind     complement(8222..8241)
                     /label=PIRES2-EGFP.P3¡¯
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(8259..8280)
                     /label=pLVX-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(8271..8290)
                     /label=IRES-R
                     /note="color: #a020f0; direction: LEFT"
     CDS             8769..9368
                     /codon_start=1
                     /label=PuroR
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9387..9975
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #a6acb3"
     primer_bind     complement(9422..9444)
                     /label=PCDNA3.4-R
                     /note="color: #a020f0; direction: LEFT"
     CDS             complement(9858..9869)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     LTR             10050..10283
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(10454..10473)
                     /label=LINK1.F
                     /note="color: #a020f0; direction: LEFT"
     promoter        10488..10592
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             10593..11453
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11624..12212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     12475..12494
                     /label=SV40pro_F
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(12498..12509)
                     /label=SV40pro_F
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    12623..12644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     12629..12649
                     /label=3.0rev
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     12653..12672
                     /label=1300-P1
                     /note="color: #a020f0; direction: RIGHT"
     promoter        12659..12689
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
     protein_bind    12697..12713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     12702..12725
                     /label=M13R(-48)
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     12702..12723
                     /label=PQE30-F
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     12721..12737
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     12772..12791
                     /label=PIRES-R
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     12787..12806
                     /label=EBV-R
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     12795..12814
                     /label=PCMV-R
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catggccttt
     3481 gataagccta gacccaaacg gaggcaggcc aggtctgatt tttacatcac tgacaaggct
     3541 atcatggggg gctactttaa tctggcccag ctgaatttct ttaagaccct gatggagatc
     3601 ttcacaaaag ctggcatcga tgtgagcaaa atcaagcagg ataacagccc taagtacctg
     3661 atgattttga ttaagaaact gacccatgat gatgaaaaga ttgatgacaa gaagtgggcc
     3721 gatgccctgg atctgagcaa cgaatgtctc ctgaagctgc agcagctgct ctataaacat
     3781 ttccccttcc tgggaccagt gatgggcgga gaggcttcct acaatatcta caagctgaag
     3841 gacggccacc ctgaagtgaa ggtggccaat gacgtcatga gaggcatgaa gctggaggac
     3901 tgcctggctg tgctgaggca tttcgcactc tgcctgaatg actgtgccaa cttttataca
     3961 gcctttaacc catataactc tattgacgcc cagaaggagc agtatttgag ccaaaataag
     4021 gtggctgttt ggctggaaaa ggtgaaggat gcaagcagga gaatcgtgaa gcagaacaat
     4081 cagctgacct ctcaggacat ggaatttctg accggcatcg accacatgaa gccccaggac
     4141 aaggtggatg acttcggcaa catcatgacc gacaagaaag gctacaccat taaggagtac
     4201 gtggagtatg aggattatta ttttcgcatc aggggaaaac gctatctggt ggatgcagcc
     4261 ggcgccaaac tggccgatca ggaaccacga aatgccctct ctgacttcgg cgtggtgttc
     4321 ctgtgcaccc tgtttctcga aaatgatcag agcagaagaa tgctcgacga gctgagactg
     4381 ttcgagactg gtccttacga cggcagcagg gacggagatg agttcaagaa cgacatcctg
     4441 agggagattc tgctgttcta cagagcccgc atcccccgcg gaaagaggct tgatcctatg
     4501 gatgacacta cactgctcgc catggacatg ctcaacgagc tgagaaagtg cccaatgcct
     4561 ctgtacgacg tgctgtctag ggagggccag cgcttcttcg aggacgaggt gaaacaccct
     4621 aacgacctca ctcccgaggt tgccaagcgc ctcaggagtt ccgataggtt cccatacctg
     4681 gccctgcgat acattgatct gaataagtgt ttcgataaca tccgcttcca ggtgcagtta
     4741 gggaagttca ggtacaaatt ttacgataag accactattg acggtgagca ggtggttaga
     4801 ggcctccaga aggaagtgaa cgcctacggc cggttccagg atgtggagca ctacagacag
     4861 gaaaagtatg cagatatgct gcagcagact gagctggtgg aaactggcga ggaggatatc
     4921 accattgcca atttcatccc cgatactccc cagtctagcc cctacctgag tgataggacc
     4981 gctagttaca atattcacaa caaccgcatc ggcctccttt ggaacatgcc cggagagcac
     5041 gaagttctga ccggcgatga gaaaatgtac ctgcctgacc tgaatgtgga cgacaacggc
     5101 aaggccgatg tgttcctgcc cgcccctaaa gcctctctgt ccgtcaggga cctgcccgct
     5161 cttgtgtttt atctgtatct ccaaaatcag caccccgacc tgacaccagc tgaggccatt
     5221 atccagcaga agtataatgc cctggtgaga tttttcgagg atgtcagcac aggcaggctc
     5281 ctgcccgtga aaggaattgc tgagctggag gccgccatcg acaagtatgg acacctgacc
     5341 gtgcacgaaa ttccagaaaa gctgcgcgac tacctcgccg gagtggccgg aactgaagat
     5401 gacgacgact gcgccgatcg acttgactgc tacgcaatgg gcattctgga aaagagatat
     5461 agacaggtgg ccgccaggat cgaccagctg aaggaggcca gaaagatggt tggcgacaaa
     5521 atgaacaggt atggcaaaaa gtcctacatc gacgtgagac ccaggagact ggcagagcag
     5581 ctggcacagt ctatcgtgga gtggcagcca gccactgata atggacgcca caaaatgacc
     5641 gggaagaact tccagcggct gatcgccttt ctggctcgct atatcgacga ggtgcctctc
     5701 caggagctga aagacctgat gacaagggcc cgcctgctgg aggggccagt ggcccatccc
     5761 tttctgcagc aagtgttgga caagaatccc ctgaacatcg aggtgctgta tgatctctat
     5821 tttaaagagg aaaaaagata cctggacaag tacatcgata ttgagatcaa caaacagggc
     5881 gaggaggtat attggctgaa ggctgacgcc gatctgtcta aaatcccctt cctgaatagc
     5941 tctaggctga ggttccacaa acgggatagc gccagcttcc agcgccttgc cgccaggtac
     6001 ctccaggtgg acggacgcca gtccactatt ctgctcccag atggactttt cacccctcat
     6061 attctgagtg ccctgaagtc cactctgtac caggaaaacc atgacctgca ggatcacctt
     6121 caggatcagg agctgaacca gaatgcctct tatcttatgt ccgtgtatct ggagcatgtg
     6181 ctgcatgact ctgcccagcc tttttacctg tcaaacagca atgattcccc cttcgcccgg
     6241 agatatgatc tcttcgctat cgtgcagaat aagaagagag gcgctgaact gcttccaggc
     6301 cctctgaccc cagtggagat caataggcgc ctgacagcta agacaccaga cggctccgca
     6361 aagctcattg aacgtgacat cgttgccctc ctggaggaca tggcccgcaa gaccgagcag
     6421 agaattcaca agctgcccca ggatctctat ctgaaaaaga agaagcaggc ccaggaggag
     6481 cggattaagc tgcagcgccg cctgaaacac aagatcacag atgtgaagaa gaacgagcgc
     6541 gccattcgga gatacaaaac tcaggatatg gcaatcttcc tgatggccca gcagatgctc
     6601 gccaaccaac tgtcttggca gaatcaggaa ggagaggtgc ccctgaagct ggccaacgtg
     6661 tgcaatgacg gcttcctgag ccagaccatg aaggtgagct ttccttacga agtgccactg
     6721 aaagaggaca agactaagaa agtgacaatt gatattgtgc aggacaatat gtccctgaag
     6781 aactacggcc tgctgtataa gctgtaccac gacgagcgcg tggtgacact gctgcagaac
     6841 atcgtggacc agcagcagat ttctttcgcc gagctgactg cggagctgag cctctatgac
     6901 cagaacagga gcaaggcctt ctccaacttt cagcagtttg agaaactggc ctttgaaacc
     6961 aatagggaag cactgtcaaa ccccgagcat agaggattct tcctcgaaaa ggtgaataag
     7021 aaaaaccagg tcatcaggta cgagcggagg aacaattttg atgctctcct ggacctgctg
     7081 gatgccatcg aggcccacca gctggaccct cgcgatagac agtggctgac atctgtggcc
     7141 aacgcctttt gtgccaacag ctattacaag attgacatgc agcagatcga gaaaaagctg
     7201 cctaccatca tccagcaaat cgtggaaatt gtgcagagga tcacagataa agccagccac
     7261 cccagcaata ggagtctaga ggcgctaccg gtcgccacca tggtgagcaa gggcgaggag
     7321 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     7381 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     7441 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     7501 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     7561 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     7621 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     7681 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     7741 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     7801 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     7861 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     7921 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     7981 gccgggatca ctctcggcat ggacgagctg tacaagtcga attctaagcc aggattccat
     8041 atgaagcgac ctgccgccac aaagaaggct ggacaggcta agaagaagaa acaacgcgtg
     8101 cagctaagcg actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag
     8161 gatgacgatg acaagtaagg atccctcccc cccccctaac gttactggcc gaagccgctt
     8221 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     8281 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     8341 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     8401 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     8461 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     8521 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     8581 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     8641 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     8701 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag gatccaccgg
     8761 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     8821 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     8881 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     8941 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     9001 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     9061 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     9121 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     9181 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     9241 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     9301 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     9361 gtgcctgacg cccatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     9421 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     9481 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     9541 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     9601 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     9661 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     9721 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     9781 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     9841 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     9901 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     9961 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
    10021 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
    10081 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
    10141 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    10201 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    10261 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
    10321 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
    10381 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
    10441 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10501 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    10561 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    10621 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    10681 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    10741 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    10801 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
    10861 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    10921 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    10981 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11041 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11101 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11161 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11221 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11281 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11341 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11401 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11461 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11521 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    11581 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    11641 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    11701 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    11761 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    11821 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    11881 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    11941 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    12001 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12061 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12121 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12181 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12241 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12301 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12361 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12421 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
    12481 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    12541 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
    12601 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    12661 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    12721 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
    12781 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    12841 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    12901 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    12961 attgtatttg ttaaatatgt actacaaact tagtagt
//
