LOCUS       pHAGE-IRES-puro-       13108 bp DNA     circular SYN 29-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13108)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13108
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #a6acb3"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..7383
                     /codon_start=1
                     /label=Bba2Cas13b
                     /note="color: #993366"
                     /translation="MSKIMCIFAKLNIQMPSNNNSLNQFITTMDNNETSIKQFVLLGED
                     NSQYRDYAGSYFTKANLNFYRTMKTVFRRSGIMKDNRLGNVEWRDDNMETALSSFDITT
                     ISATQMSSLSDHLFRNLPMFGPIMSHDKNAFLSPLKRAKKTFDKAKKDLKNAKKTHQDE
                     VKYEKLKKEAEKEYNQQKENFKNEMYGTEAKDMWDKLINIAGALLYYANLYTAKNPYST
                     EDDENKQKEREIKIAKWMNSVFEGEKGILIERKKYKYEDTLFLIDHQKETDRMEKRGNK
                     YVERDNFYFKIKILDENNKCLGLTDFGRFFFCSLFLRRSDSELYAKSVKLFENSPYCLT
                     EQELKIKQAEENKRASTESQKSDHQVNPKTIDKTESAQINIIREMMQMHRIRIPREKKI
                     DFTPSESMLLMDILNELRRCPQELYDTFNQTDKLKFVVDNPNEDPESDSKITLTRSKDR
                     FAHLALRLIDHTDALGSIRFHLRLGLFRFRFYDKKLIDNSETLRRVHKEINGFGKWHVV
                     ESLRREKWGKYNQKFELNDDGLLQFVPDSAESDPYITDWQTTYNIHANRIGLFWNGNSQ
                     SEDYYIPELKTMVDENSKVIAPVEMKIPLCSLSIYDLPAILFYTYIFNLYNGNRLGLPN
                     AEKIIKNKYTALNEFFGFAKKSESTPEELIKKQEELNLHDSEIPERLLYLMGKIKTPKG
                     KMAEWKPNSKRNNHAKELLKKIIEEDEWRIKSFKDKKNKIEDKNRYGRKDYSDIRHGVL
                     AKYLVESMMMWQPGKQKAETKISSANYRALRDYLSNYNNSSNNLQYVLEAAHLIDSENP
                     HPFIDKVKGSNIELYYTNYLHEELKHARGILAKLEDNNYTGDKKDIIPPFAKPESDKWN
                     KKSNTIQSCAAKYLEHPLQLPDGLFTQAIIDLLKAADIDETIKNDATKDSNATYIIRYW
                     FDKVGQDSPQLFYDSTLFKRSYKFIDKCFLQKDEGNQTIKHFLTEKEILDNIKIFRGET
                     GSLAGKLYPKDKLEESKKSIINQLNEVKDKEKAIRRYRVQDIALFLLARKRLAELLSKS
                     ENSNRNEARIETSLSQNNISKKANNLKLKDFGFDEDFHFLSGSEDNGDGLLFEYEYIVD
                     NDANKKIMIVQEGLSLKNYGNIYRVLGDNRIKTLMQLLYEIKKDKVEDGTITFTFAELV
                     KELTNNDKLHHEFYRLFQNIEQEAYDGTGGFDANKKNKEVLDNPDDEQFFFVRQRKNGE
                     VTTKEAVRNSFKKLIELLKQYSTDEGIIVQLANDAIADRYPKPDAEMELKETGVTESLI
                     AYLNNLIKKNQKQLKNQEQR"
     primer_bind     complement(7226..7239)
                     /label=Bba2-T-XbaI-R
                     /note="color: #a020f0; direction: LEFT"
     CDS             7235..7264
                     /codon_start=1
                     /label=Bba2Cas13b
                     /note="color: #993366"
                     /translation="SWPMMPSPTA"
     primer_bind     complement(7384..7407)
                     /label=Bba2-T-XbaI-R
                     /note="color: #a020f0; direction: LEFT"
     CDS             7411..8127
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             8155..8202
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8221..8286
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8293..8860
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             8880..9479
                     /codon_start=1
                     /label=PuroR
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9498..10086
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #a6acb3"
     CDS             complement(9969..9980)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     LTR             10161..10394
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        10599..10703
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             10704..11564
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11735..12323
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    12734..12755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        12770..12800
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
     protein_bind    12808..12824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     12832..12848
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catgagcaag
     3481 atcatgtgca tcttcgccaa gctgaacatc cagatgccta gcaataacaa ttccctgaac
     3541 cagttcatca ccaccatgga caataatgag accagcatta agcagttcgt gctgctgggc
     3601 gaggacaata gccagtaccg ggactacgcc ggctcctact tcaccaaggc caacctgaac
     3661 ttctatagga ccatgaaaac cgtgttcagg agaagcggaa tcatgaagga taacaggctg
     3721 ggcaacgtgg agtggcgcga cgacaacatg gagacagccc tgtccagctt tgatatcaca
     3781 accatcagcg ccacacagat gagtagcctg agcgaccacc tgttccgcaa tctgcctatg
     3841 ttcggaccta tcatgagcca cgacaagaat gccttcctgt cccctctgaa gcgggccaaa
     3901 aagacctttg acaaggccaa gaaggacctg aagaacgcca aaaagacaca ccaggacgag
     3961 gtgaagtacg agaagctgaa gaaggaggcc gagaaggagt acaaccagca gaaggaaaac
     4021 tttaaaaacg agatgtacgg gactgaggcc aaggacatgt gggataagct gatcaacatc
     4081 gccggcgctc tgctgtacta cgccaacctg tacaccgcca agaaccccta cagcaccgag
     4141 gacgatgaga ataagcagaa ggaacgggag atcaagatcg ccaagtggat gaacagcgtc
     4201 tttgagggcg aaaagggaat cctgattgag cgcaaaaagt acaagtacga agataccctg
     4261 tttctgattg accaccagaa ggagaccgat cgcatggaga agagaggcaa caaatacgtc
     4321 gagcgggaca atttctactt taagattaag atcctggacg agaacaataa gtgcctgggc
     4381 ctgactgatt ttgggcgctt cttcttttgc agcctgtttc tgcggcgctc cgacagtgag
     4441 ctgtacgcca agtccgtgaa gctgtttgag aactccccat actgcctgac tgagcaggag
     4501 ctgaagatta agcaggccga ggaaaacaag agggcaagta ccgagagcca gaagtctgac
     4561 caccaggtga accccaagac tatcgacaag acagagagcg cccagattaa catcatccgc
     4621 gagatgatgc agatgcacag gatcagaatt cccagagaga agaagattga cttcaccccc
     4681 agcgagtcca tgctgctgat ggatattctg aacgagctgc gcagatgccc acaggagctg
     4741 tatgacacct tcaatcagac agacaagctg aagttcgtgg tggacaaccc taacgaggac
     4801 cctgagtctg acagcaagat cactctgact cggagcaagg accgctttgc ccacctggcc
     4861 ctgaggctga tcgaccatac tgatgcactg ggcagtatca gatttcatct gcggctggga
     4921 ctgttccgct ttaggttcta cgataagaag ctcattgata atagtgagac tctgcgccgc
     4981 gtgcacaaag agatcaacgg cttcggcaag tggcacgtcg tggagtccct ccggcgggag
     5041 aagtggggaa aatacaacca gaaattcgag ctgaatgacg acggcctgct gcaattcgtg
     5101 cccgacagcg cagagtccga cccatacatc accgactggc agaccaccta caatatccac
     5161 gccaacagga tcgggctgtt ctggaacggg aactctcagt ctgaggacta ctacatccct
     5221 gagctcaaga ccatggtcga cgagaatagc aaagtgattg cccccgtgga gatgaagatc
     5281 ccactctgta gcctcagcat ctacgatctg cccgctatcc tgttctacac ttacatcttc
     5341 aatctgtaca atggaaaccg gctgggcctg ccaaacgccg agaaaattat caagaataag
     5401 tacaccgccc tcaatgagtt ctttgggttc gccaagaaaa gcgaaagcac acccgaggag
     5461 ctgatcaaaa agcaggagga gctgaacctc cacgacagcg agatccctga gcgcctgctc
     5521 tatctgatgg ggaagatcaa aacccctaag ggcaagatgg ccgagtggaa acccaactcc
     5581 aagcgcaaca accacgccaa ggagctgctg aagaagatta tcgaggagga cgagtggagg
     5641 atcaagagct tcaaggacaa gaaaaataag attgaggaca agaaccggta tgggagaaag
     5701 gactactccg acatccggca tggcgtgctg gcaaagtacc tggtggagag catgatgatg
     5761 tggcagcccg gcaagcagaa agccgagaca aagatcagca gtgccaacta ccgcgctctg
     5821 agggattatc tgagtaacta caacaatagc tccaacaatc tgcagtatgt gcttgaagct
     5881 gcccacctga ttgattccga gaacccacac ccttttatcg acaaagtgaa ggggagtaac
     5941 atcgagctgt actacactaa ttatctgcac gaggagctga aacacgcacg gggcatcctg
     6001 gccaagctag aggacaacaa ctacaccggc gacaagaagg acatcatccc ccccttcgcc
     6061 aagcccgaat ccgacaagtg gaataagaaa agcaacacca tccagtcctg cgccgccaag
     6121 tatctcgagc accccctgca gctgcctgac gggctgttca cccaggccat cattgacctg
     6181 ctgaaggcag ccgacatcga cgagaccatc aagaatgatg ccaccaagga tagtaatgcc
     6241 acctacatca tccgctattg gttcgataag gtgggccagg actcccccca gctgttctac
     6301 gacagcaccc tgtttaagag gtcctacaag ttcattgata agtgctttct gcagaaagat
     6361 gagggcaatc agaccatcaa acacttcctt actgagaagg agattctgga caatatcaag
     6421 atcttccggg gagagaccgg ctccctggcc ggcaagctgt accccaagga taagctggag
     6481 gagtctaaga agtccatcat caaccagctg aatgaggtga aggacaaaga gaaggccatc
     6541 cgccggtatc gggtgcagga tatcgccctg tttctgctgg cccggaagcg gctggctgag
     6601 ctgctgagta agtctgagaa ctccaaccgg aatgaagctc gcatcgagac tagcctgagc
     6661 cagaacaata tcagcaagaa ggccaataat ctgaaactga aggatttcgg atttgacgag
     6721 gattttcatt tcctgtccgg aagcgaggat aatggggacg ggctgctgtt cgagtacgag
     6781 tacatcgtgg ataacgatgc taataagaag atcatgatcg tgcaggaggg cctgagcctg
     6841 aagaactacg gaaatatcta tagggtgctg ggcgataacc gaattaagac cctgatgcag
     6901 ctgctgtacg agatcaagaa ggacaaggtc gaggacggca ccatcacttt caccttcgcc
     6961 gagctggtga aggagctcac aaataatgac aagctgcacc acgagttcta caggctgttc
     7021 cagaacattg agcaggaggc ctacgatggc accggcggct tcgatgccaa taagaagaac
     7081 aaagaggtgc tggataatcc tgacgacgag cagttctttt tcgtgagaca gcggaagaac
     7141 ggggaggtga ccaccaagga ggccgtgagg aactctttta agaagctgat cgaactgctg
     7201 aaacagtata gcacagatga gggcatcatc gtgcagctgg ccaatgatgc catcgccgac
     7261 cgctacccta agcctgatgc cgagatggag ctgaaggaga ccggcgtgac cgagagcctg
     7321 atcgcctatc tgaacaacct gatcaagaag aaccagaagc agctgaagaa ccaggagcag
     7381 agaagtctag aggcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     7441 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     7501 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     7561 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     7621 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     7681 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     7741 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     7801 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     7861 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     7921 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     7981 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     8041 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     8101 actctcggca tggacgagct gtacaagtcg aattctaagc caggattcca tatgaagcga
     8161 cctgccgcca caaagaaggc tggacaggct aagaagaaga aacaacgcgt gcagctaagc
     8221 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
     8281 gacaagtaag gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     8341 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     8401 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     8461 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     8521 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     8581 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     8641 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     8701 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     8761 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     8821 ccacggggac gtggttttcc tttgaaaaac acgatgataa ggatccaccg gagcttacca
     8881 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     8941 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     9001 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     9061 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     9121 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     9181 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     9241 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     9301 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     9361 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     9421 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     9481 gcccatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     9541 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     9601 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     9661 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     9721 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     9781 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     9841 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     9901 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     9961 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
    10021 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
    10081 ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
    10141 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
    10201 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    10261 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    10321 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    10381 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
    10441 gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt
    10501 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
    10561 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    10621 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    10681 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    10741 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    10801 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    10861 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    10921 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
    10981 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    11041 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    11101 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    11161 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    11221 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    11281 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    11341 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    11401 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    11461 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    11521 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    11581 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    11641 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    11701 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    11761 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    11821 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    11881 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    11941 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    12001 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    12061 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    12121 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    12181 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    12241 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    12301 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    12361 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    12421 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    12481 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    12541 ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc
    12601 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    12661 aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg
    12721 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    12781 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    12841 gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    12901 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    12961 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    13021 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    13081 gttaaatatg tactacaaac ttagtagt
//
