LOCUS       pHAGE-IRES-puro-       12765 bp DNA     circular SYN 29-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12765
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #a6acb3"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff"
     promoter        2242..2453
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
                     /note="color: #ffffff"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     3450..3491
                     /label=Pba5-NheI-F
                     /note="color: #a020f0; direction: RIGHT"
     CDS             3472..7041
                     /codon_start=1
                     /label=dPba5Cas13b
                     /note="color: #993366"
                     /translation="MMVEFDKNRKYIYAAYCNMAQHNFISTLKHIAKCLSLDLELTDKS
                     KKIIDELKDKVNSLTQDKKLKFYELLNKHFPFLAPMIDSDKSAKDYNEIKAINDILPQI
                     GTVVDFYADYTTAYDPDEEKSVDYLHPSEQTLVPRLKELLKAEIRTVQQRFNYVEDEVL
                     FIKNKEMDKERYRYCLFKKLCRTDKKVFSRRGLVFFISLFLEKKYINEMLNKTKDFYAK
                     DDLFNDKRKHIIFESLAVYRIRLPRNRYESQQDDVGLALDMVNELQRCPKELFDLLSTE
                     DQIKFRPDASDEDSSKTAIMIRHADRFPKLAMNYIDQCNVFNSIRFQVNLGKYRYAFYN
                     KKCIDGLSHVRSLQKELHGFGKLNEIEKARLDTWGNLIRDFEDIKTNTIDTMPYINDQR
                     ASYIINNNRIGLYWEDKDDVNEPKLVLPKLPNEPTAKNLRQQKANGERIVLLQSPKCFM
                     STHELPALIFYHIIRKKHQKTDSPDVESIIIKWVEGFRNFIQEVMDGKITSQNAQERAN
                     QLGINYAQHLPKKIQEYLDANQDNNNAITANLQKKLKNRLTTHIDETKKLIEKYNNVKH
                     VDSKKNKRGTKRFVEIKQGRLGAWLAQDIIALQPAPADGNNKLTGLNFQLLQSALSIYD
                     NFDNLKRLLIAAHLIQSKGNHPFLSEIVSKSPQTIGDFYQAYLKAKLNWLNSLLSEEDL
                     IKYPFLTRGVNKWKFRDEEYYKSIMQQYLELPAELPRGLFETAIKDILIDCFGRGFVRG
                     NNREEANVAFLIAKYFKKVFNDDYQEFYTQPDGNYKRHYYFFKLLLQSDKDYGKTVKEI
                     EDILRTDMSLTLLSKTTIENDDDSDNSDNSDLKEYFEEINKKLAKSLAKDPTLDKRHRI
                     EDIISKSETLSKLPNSQQKKLLRISSKRHGVPINDGLVKKHIKKITSNSEKEEELKKLS
                     HALHKLKSTERLIRRYRVQDIIIFMMAREILFENDKNLFENRFEEFKLKNIRPIRRQEG
                     VGALEIKVPFAITLHIKGSNESVVIRQKSIKLKNYGDFHKFLYDNRIETLIPYLRDDAN
                     AVKVTIDRDELEHEFETYDKVRLDVFESVHKIERLILERYPELHDKRSSLYYFEKKGVE
                     KPIPKKNNFRQLIECEPQFAVLDKDDCLNIANSFSANSYNSKDHEKVNIPSGKIGEIAE
                     LISERMKTCLNNFEKKTRQENDSE"
     primer_bind     complement(3785..3829)
                     /label=Pba5-R112-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     3795..3835
                     /label=Pba5-R112-F
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(6860..6905)
                     /label=Pba5-R1138-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     6871..6914
                     /label=Pba5-R1138-F
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(7022..7065)
                     /label=Pba5-XbaI-R
                     /note="color: #a020f0; direction: LEFT"
     CDS             7069..7785
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7813..7860
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     7845..7860
                     /label=Pba5-NheI-F
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    7950..8517
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             8537..9136
                     /codon_start=1
                     /label=PuroR
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9155..9743
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #a6acb3"
     CDS             complement(9626..9637)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     LTR             9818..10051
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        10256..10360
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             10361..11221
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11392..11980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    12391..12412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        12427..12457
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
     protein_bind    12465..12481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     12489..12505
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catgatggtg
     3481 gagttcgata agaatagaaa gtacatctac gccgcctact gtaacatggc ccagcacaac
     3541 ttcatcagca ccctgaagca catcgccaag tgtctgtctc tggatctgga gctgaccgat
     3601 aaaagcaaga aaatcatcga cgagctgaag gataaggtga acagcctgac acaggataag
     3661 aagctgaagt tctacgaact gctgaacaag cacttccctt tcctggcccc catgattgac
     3721 agcgataaga gcgccaagga ttacaacgag atcaaggcca tcaatgacat cctcccacag
     3781 atcggcaccg tggtggattt ctacgccgac tacaccaccg cctacgaccc cgacgaggag
     3841 aagtccgtgg actacctgca tcccagcgag cagaccctgg tgcccagact gaaggagctg
     3901 ctgaaggctg agatccgcac cgtgcagcag aggtttaact atgtggagga tgaggtgctg
     3961 ttcattaaga acaaggagat ggacaaggag agatataggt actgcctgtt taagaagctg
     4021 tgccggaccg ataagaaggt gttctcaagg aggggcctgg tgtttttcat cagcctgttc
     4081 ctggagaaga aatacattaa cgagatgctc aataagacca aggactttta cgccaaggac
     4141 gacctgttca acgataagcg caaacacatt atctttgaat ctctggccgt gtacagaatt
     4201 aggctgccca ggaatcggta cgagagccag caggatgacg tgggcctggc cctggacatg
     4261 gtgaacgagc tgcagcggtg ccctaaggaa ctgttcgacc tgctgtccac cgaggaccag
     4321 atcaaattca gaccagacgc ttctgatgag gactcctcca agacagccat catgattcgc
     4381 catgccgacc ggttccctaa gctcgccatg aactatattg atcagtgcaa tgtgtttaac
     4441 tccatcaggt tccaggtgaa cctgggcaag taccgctacg ccttctacaa caagaagtgt
     4501 attgacggcc tgagccatgt gaggtccctg cagaaggagc tgcacgggtt cgggaagctg
     4561 aatgagattg agaaggctag gctggacaca tggggaaacc tgatccggga cttcgaggat
     4621 atcaagacca acaccattga cacaatgccc tacattaatg accagagagc cagctacatc
     4681 attaacaaca accggatcgg cctgtactgg gaggataagg atgacgtgaa tgagcctaag
     4741 ctcgtgctgc caaagctgcc taatgagcct accgccaaga acctgcggca gcagaaggcc
     4801 aacggcgagc ggatcgtgct cctgcagtct cccaagtgct tcatgtccac ccacgagctg
     4861 cctgctctga tcttctacca catcatccgg aagaagcacc agaaaaccga tagccctgac
     4921 gtggagtcca tcattatcaa atgggtggag gggttccgga actttatcca ggaggtcatg
     4981 gatggcaaga ttacctccca gaacgctcag gagcgggcca atcagctggg cattaactac
     5041 gcccagcatc tgcctaagaa gatccaggag taccttgatg ccaaccagga taacaataac
     5101 gccatcaccg ccaatctgca gaagaagttg aagaacagac tgaccaccca cattgatgag
     5161 accaagaagc tgatcgaaaa gtacaacaac gtgaagcacg tggactcaaa gaaaaataag
     5221 cgcggcacca agcggttcgt ggagatcaaa caggggcgcc tgggcgcctg gctggcccag
     5281 gacattattg ccctgcagcc tgcccctgcc gacggcaaca ataagctgac cggcctgaac
     5341 tttcagctgc tgcagagcgc cctgagcatc tatgataact ttgacaacct gaagcggctg
     5401 ctgatcgccg cccacctgat ccagtccaag ggcaatcacc cctttctcag cgagattgtg
     5461 tctaagagcc ctcagaccat cggggacttc taccaggcct acctgaaggc taaactgaat
     5521 tggctgaact ccctgctgag cgaggaggat ctgattaagt acccttttct gacaaggggc
     5581 gttaacaagt ggaagttcag ggacgaggag tattacaaga gcatcatgca gcagtacctg
     5641 gagctccccg ccgagctgcc cagaggcctg tttgaaacag ccatcaagga catcctgatc
     5701 gactgcttcg gccgcgggtt tgtgaggggc aacaatcgcg aggaggccaa cgtggccttc
     5761 ctgatcgcca agtatttcaa gaaggttttt aacgatgact accaggagtt ttatactcag
     5821 cccgatggca attacaagag gcactactac ttcttcaaac tgctgctgca gtccgataag
     5881 gactacggca agaccgtgaa ggaaatcgag gacatcctgc gcaccgacat gagcctgacc
     5941 ctgctgagta aaaccaccat cgagaatgac gacgacagtg acaactctga taacagcgac
     6001 ctgaaggagt actttgagga gatcaataag aagctggcca aaagcctcgc caaggacccc
     6061 actctggaca agaggcacag gatcgaggac attatcagca agagcgagac cctctctaag
     6121 ctgccaaact cccagcagaa gaaactcctg aggattagca gcaagcgaca cggcgtgccc
     6181 atcaatgacg gcctggtgaa gaaacacatc aagaagatca cctccaactc cgagaaggag
     6241 gaggagctga agaagctgag ccacgccctg cacaagctga agtctacaga gaggctgatc
     6301 aggagatata gagtgcagga catcatcatt ttcatgatgg ctagggagat cctgttcgag
     6361 aacgataaga acctgtttga gaatagattt gaagagttca agctgaagaa catcaggcct
     6421 attaggaggc aggagggcgt gggagctctg gagattaagg tccccttcgc catcaccctg
     6481 cacatcaagg gcagcaacga gagcgtagtg atccggcaga aaagcatcaa gctgaaaaac
     6541 tacggcgact tccacaagtt tctgtacgat aaccggatcg agaccctgat cccttacctg
     6601 cgggacgacg ccaatgccgt gaaggtgacc attgataggg atgaactgga acacgagttt
     6661 gagacctatg ataaggtgag gctggacgtg tttgaatctg tgcacaagat cgagaggctg
     6721 attctggagc ggtaccccga gctgcacgac aaaaggagct ccctgtacta ttttgagaag
     6781 aagggcgtgg agaagccaat ccccaagaag aataatttca ggcagctgat cgagtgcgag
     6841 ccccagttcg ccgtgctgga caaggatgac tgcctgaaca tcgccaacag cttcagcgcc
     6901 aactcctaca attctaagga ccatgagaag gtgaacatcc caagcggcaa aatcggcgag
     6961 atcgccgagc tgatctccga gcggatgaaa acctgcctga acaacttcga aaagaaaacc
     7021 cgccaggaga atgactccga gagtctagag gcgctaccgg tcgccaccat ggtgagcaag
     7081 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     7141 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     7201 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     7261 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     7321 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     7381 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     7441 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     7501 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     7561 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     7621 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     7681 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     7741 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtcgaa ttctaagcca
     7801 ggattccata tgaagcgacc tgccgccaca aagaaggctg gacaggctaa gaagaagaaa
     7861 cacgcgtgca gctaagcgac tacaaagacc atgacggtga ttataaagat catgacatcg
     7921 attacaagga tgacgatgac aagtaaggat ccctcccccc cccctaacgt tactggccga
     7981 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     8041 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     8101 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     8161 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     8221 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     8281 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     8341 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     8401 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     8461 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagga
     8521 tccaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     8581 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     8641 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     8701 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     8761 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     8821 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     8881 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     8941 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     9001 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     9061 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     9121 caagcccggt gcctgacgcc catcgataga tcctaatcaa cctctggatt acaaaatttg
     9181 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     9241 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     9301 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     9361 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     9421 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     9481 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     9541 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     9601 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     9661 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     9721 ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct
     9781 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     9841 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     9901 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9961 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
    10021 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
    10081 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa
    10141 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
    10201 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
    10261 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    10321 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    10381 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    10441 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    10501 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    10561 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
    10621 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    10681 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    10741 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    10801 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    10861 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    10921 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    10981 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    11041 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    11101 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    11161 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    11221 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    11281 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    11341 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    11401 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    11461 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    11521 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    11581 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    11641 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    11701 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    11761 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    11821 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    11881 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    11941 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    12001 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    12061 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    12121 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    12181 gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt
    12241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    12301 ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga
    12361 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    12421 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    12481 atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc
    12541 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    12601 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    12661 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    12721 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
