LOCUS       pHAGE-IRES-puro-       12465 bp DNA     circular SYN 18-JUL-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12465)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12465
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #a6acb3"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff"
     promoter        2242..2453
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
                     /note="color: #ffffff"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     3409..3466
                     /label=Primer 1
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     3413..3437
                     /label=Primer 2
                     /note="color: #a020f0; direction: RIGHT"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     3449..3491
                     /label=Pba-NheI-F
                     /note="color: #a020f0; direction: RIGHT"
     CDS             3472..6740
                     /codon_start=3
                     /label=PbaCas13b
                     /note="color: #993366"
                     /translation="SMKIPDLINNYKEQFGTYAVMALMNAQTVLDHIQKLACIGNSESY
                     KEDLWKHPVMLHIKNAAYETDNNPETTLFVMEKLQFYFPFLKIMAERQREYSNKKYNEN
                     RLAVNSRDLFFVLDKVFRVLKNYANTTAAFMTKSLSENEEYLFLKNEQPLANMVNEYYT
                     VALRDMKDRYSYSTEDLAFIQDNRYKQTRGDDGKRKATVNLDFFLSLQSHNGDPAKKLH
                     LSGVGVALLICLFLEKQYVNLFISKLSIFSTYKPNSEQHRIICRSMGIHSIKLPKDRIH
                     SLKDGMSVAMDMLNETKRCPKELFDTLSADKQARFRTISDDHNEVLLMRSSDRFAQLLL
                     QYIDYNKLFKGIRFHVNMGKLRYLFNAAKNCIDGQTRVRVIEHQLNGYGRIDEIEAERK
                     SEDGKFANSGISIRDFENVRRDDGNPENYPYVVDTYSHYILENNKVEMQFTDSCILPFI
                     EEYGGKWYVAKDVPACRISTLELPALAFHMLLLGSKKTEERIKEVYGKYMKLFEAMSND
                     AVTKENIESFGIALWNLPQKAIDAINGCAHAKNVNAFIKNTVEEMLADTEKRLERLKED
                     RKGISSHDNKMGKPNFRQISTGRLADFLAKDIVKLQPATTEDGSDKMTGLNYRVMQAAI
                     ATYNSNGNPEAEQQFRGMFEKARLIGKSPNAHPFLYKVFASALPKNTIEFYDKYLRERK
                     RYLSNLTKDIESGKTASVPFVNRNQNKWKKPNQEYLGQTYGNSQNLAIELPRQMFDADI
                     KAFLKGLPEMKDVDFDNANVTFLIGEYMKRVLADDFQNFYAWKRNYRYMDMLMGETDSK
                     DGLRHMFTSVEEREKLWREREGRTSSYRKWAARKNQGCGYSKRLPDNVLDEILAKRLSG
                     SRNDYQKCEKAIRRQKVQDAMLFMLAKKAFTQHADFEGKDFKLKNIMPDADKGILSETM
                     PMTFTFEKGGKQYTIESGGMKLKNYGDFHALANDRRIGCLMKLIGTNTAQKEDLISEFN
                     KYDQCRPQVVKLVLDLEKWAFAKYPELGASEEMSYVSFDKILNKLTQHRNLGNRQSNIL
                     RKIRNAFDHNSYPESGIVEITTLPEIAQNMKDMFGRYAIVK"
     primer_bind     4029..4048
                     /label=Pba-seq-F1
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     4682..4701
                     /label=Pba-seq-F2
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     5334..5353
                     /label=Pba-seq-F3
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(6721..6767)
                     /label=Pba-XbaI-R
                     /note="color: #a020f0; direction: LEFT"
     CDS             6768..7484
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     7509..7531
                     /label=Primer 2
                     /note="color: #a020f0; direction: RIGHT"
     CDS             7512..7559
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     7543..7559
                     /label=Pba-NheI-F
                     /note="color: #a020f0; direction: RIGHT"
     CDS             7578..7643
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7650..8217
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     CDS             8237..8836
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8855..9443
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #a6acb3"
     CDS             complement(9326..9337)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     LTR             9518..9751
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        9956..10060
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             10061..10921
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11092..11680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    12091..12112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        12127..12157
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
     protein_bind    12165..12181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     12189..12205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag cgatccatga
     3481 agatccccga cctgattaac aattacaaag agcagttcgg cacctatgcc gtgatggccc
     3541 tgatgaacgc ccagacagtg ctggaccaca tccagaaact ggcctgcatt ggcaatagcg
     3601 agtcctacaa ggaggatctg tggaaacacc ctgtgatgct gcacatcaag aacgccgcct
     3661 atgagacaga taacaatccc gagacaaccc tgtttgtgat ggagaagctg cagttctact
     3721 ttcctttcct gaaaattatg gccgagcggc agcgcgagta ctccaacaag aaatataacg
     3781 agaatcggct ggccgtgaat tctcgcgatc tgttctttgt gctggacaag gtgtttagag
     3841 tgctgaaaaa ctacgccaat accacagccg ccttcatgac aaaatctctg agcgagaacg
     3901 aggagtatct gtttctgaag aatgagcagc cactggccaa catggtgaat gagtactata
     3961 ccgtggccct gcgcgatatg aaagacagat actcctattc tacagaggat ctggccttca
     4021 tccaggacaa caggtacaag cagacccggg gcgacgatgg caagaggaaa gccacagtga
     4081 acctggactt ctttctgtct ctgcagagcc acaatggcga tcccgccaag aaactgcacc
     4141 tgagcggagt gggagtggcc ctgctgatct gcctgtttct ggagaagcag tacgtgaacc
     4201 tgtttatctc caagctgtct attttcagca cctataaacc caattccgag cagcaccgca
     4261 tcatttgtag atccatgggc atccactcta ttaagctgcc taaagataga atccactctc
     4321 tgaaggacgg catgagcgtg gccatggata tgctgaacga gacaaagagg tgccccaaag
     4381 agctgtttga tacactgagc gccgacaagc aggccagatt caggaccatt tccgacgatc
     4441 acaatgaggt gctgctgatg aggagctccg acaggttcgc acagctgctg ctgcagtaca
     4501 tcgattacaa caagctgttt aaaggcatta gattccacgt gaatatgggc aagctgaggt
     4561 acctgtttaa cgccgccaaa aattgtatcg acggccagac acgggtgcgc gtgattgagc
     4621 accagctgaa cggctatggc cggatcgacg agattgaggc cgagcgcaag tccgaggatg
     4681 gcaaatttgc caactccggc atctctattc gggatttcga gaatgtgaga agggacgatg
     4741 gcaaccccga gaattaccct tatgtggtgg acacctactc tcactatatc ctggagaaca
     4801 ataaggtgga gatgcagttt acagatagct gcatcctgcc tttcattgag gagtacggcg
     4861 gcaagtggta cgtggccaaa gacgtgcctg cctgtcggat ctccaccctg gagctgccag
     4921 ccctggcatt ccacatgctg ctgctgggct ctaagaaaac agaggagcgc attaaggagg
     4981 tgtacggcaa gtatatgaaa ctgttcgagg ccatgtctaa cgacgccgtg accaaagaga
     5041 atatcgagag ctttggcatt gccctgtgga acctgccaca gaaggccatc gatgccatta
     5101 atggctgtgc ccacgccaag aacgtgaatg ccttcatcaa aaacaccgtg gaggagatgc
     5161 tggccgacac agagaagagg ctggagcggc tgaaggagga ccggaaaggc atttctagcc
     5221 acgataacaa gatgggcaaa ccaaattttc ggcagatcag caccggccgc ctggcagatt
     5281 tcctggcaaa ggacattgtg aaactgcagc ccgccaccac agaggatggc agcgacaaga
     5341 tgaccggcct gaattaccgc gtgatgcagg ccgccatcgc cacatataac tccaatggca
     5401 accctgaggc cgagcagcag tttagaggca tgttcgagaa ggccaggctg attggcaaaa
     5461 gcccaaacgc ccaccccttt ctgtacaagg tgttcgcctc cgccctgcca aaaaatacca
     5521 tcgagttcta cgacaagtat ctgagagaga ggaaacggta tctgtctaac ctgaccaagg
     5581 atattgagag cggcaaaaca gcctccgtgc catttgtgaa tagaaaccag aataagtgga
     5641 agaaacccaa tcaggagtac ctgggccaga cctatggcaa cagccagaat ctggccatcg
     5701 agctgccaag gcagatgttt gacgccgata ttaaggcctt cctgaaaggc ctgcccgaga
     5761 tgaaggacgt ggattttgac aacgccaatg tgacattcct gatcggcgag tacatgaaaa
     5821 gagtgctggc cgacgatttt cagaacttct atgcctggaa gcgcaattac agatatatgg
     5881 acatgctgat gggcgagaca gattccaaag acggcctgcg gcacatgttc acatctgtgg
     5941 aggagcgcga gaagctgtgg agggagagag agggacgcac ctcctcttac agaaagtggg
     6001 ccgccaggaa aaaccagggc tgcggctatt ccaaaaggct gcctgataat gtgctggacg
     6061 agatcctggc aaagcggctg agcggctccc gcaacgacta ccagaagtgt gagaaagcca
     6121 ttcggcgcca gaaggtgcag gatgccatgc tgtttatgct ggccaagaaa gccttcacac
     6181 agcacgccga ttttgagggc aaagacttca agctgaaaaa tatcatgcct gatgccgaca
     6241 agggcattct gtctgagaca atgccaatga cctttacatt cgagaagggc ggcaaacagt
     6301 acacaatcga gagcggcggc atgaagctga aaaactatgg cgacttccac gccctggcca
     6361 atgatagaag gatcggctgc ctgatgaagc tgattggcac caacacagcc cagaaagagg
     6421 acctgatcag cgagtttaat aagtacgatc agtgtcggcc tcaggtggtg aagctggtgc
     6481 tggacctgga gaagtgggcc ttcgccaaat acccagagct gggcgcctcc gaggagatga
     6541 gctacgtgag cttcgacaag atcctgaaca aactgaccca gcacagaaac ctgggcaata
     6601 ggcagtctaa tatcctgagg aagattcgga acgccttcga ccacaattct taccccgaga
     6661 gcggcatcgt ggagattacc acactgcctg agatcgccca gaacatgaaa gatatgtttg
     6721 gcagatatgc cattgtgaag ggtctagagg cgctaccggt cgccaccatg gtgagcaagg
     6781 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     6841 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     6901 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     6961 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     7021 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     7081 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     7141 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     7201 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     7261 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     7321 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     7381 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     7441 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtcgaat tctaagccag
     7501 gattccatat gaagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaac
     7561 aacgcgtgca gctaagcgac tacaaagacc atgacggtga ttataaagat catgacatcg
     7621 attacaagga tgacgatgac aagtaaggat ccctcccccc cccctaacgt tactggccga
     7681 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     7741 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     7801 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     7861 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     7921 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     7981 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     8041 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     8101 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     8161 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagga
     8221 tccaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     8281 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     8341 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     8401 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     8461 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     8521 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     8581 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     8641 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     8701 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     8761 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     8821 caagcccggt gcctgacgcc catcgataga tcctaatcaa cctctggatt acaaaatttg
     8881 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     8941 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     9001 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     9061 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     9121 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     9181 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     9241 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     9301 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     9361 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     9421 ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct
     9481 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     9541 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     9601 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9661 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9721 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     9781 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa
     9841 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     9901 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     9961 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    10021 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    10081 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    10141 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    10201 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    10261 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
    10321 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    10381 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    10441 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    10501 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    10561 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    10621 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    10681 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    10741 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    10801 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    10861 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    10921 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    10981 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    11041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    11101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    11161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    11221 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    11281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    11341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    11401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    11461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    11521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    11581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    11641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    11701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    11761 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    11821 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    11881 gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt
    11941 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    12001 ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga
    12061 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    12121 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    12181 atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc
    12241 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    12301 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    12361 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    12421 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
