LOCUS       pHAGE-IRES-puro-       12571 bp DNA     circular SYN 18-JUL-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12571
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #a6acb3"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..6846
                     /codon_start=1
                     /label=Hgm6Cas13b
                     /note="color: #993366"
                     /translation="MEDNLTKTKKKMRIPKLIEEHKSVFGAYSTMALSNVETVLNHIAE
                     RAGLDGYERDRGPGVEDYWEHPVMQCLCRKDKPRSIPSDVLLDVRNRLFRSFPFLKIMA
                     ENQRDYRNAKGKVECVEINESDIFVVLNNSFRVLKAYADTCTAYLIENRIWEDNSPMLM
                     YNECPLAAMVNQYYTAALRVTKERYGYETRDLTFIQKRRFKQEPEKEASGNVKKKAVPD
                     LAFFLSLVALNGDGRKWLHLSGWGVVLLICLFLEKKYVNVFLSKLPNPGNYPPSSKERR
                     IIRRSMGVCSVVLPKERIHSETGDLSVALDMLNELKRCPRELFDTLSPGDQERFRTISS
                     DHNEVLQMRSKDRFAQLVLQYIDHNRLFENLRFHVNMGKLRYLFNPKKYCIDGQTRVRV
                     LEHPLNGFGRLQEMEEKRLQENGPFARSGIKVRCFDEVRRDDANESNYPYIVDTYTHYV
                     LENDMVEMFFCPEGSGMKMPEVTSREGKWYVDKKVPHCRMRMSVLELPAMLFHLLLCGA
                     KNTEVHIGKVCDNYCHLFSDMAQGNLTEENILSYGIKKEDIPQKVWDCVRGVHMGKDSR
                     AYREKEIRERYEDVTRRLERLEADRKAVLGGENKIGKRGFVQIVPGRLAAYLATDICRL
                     QPSLRKGDGYGTDRLTGLNFRLLQSSIATYNCGESDILYGRFRDVFCSAGLIGGDNPHP
                     FLDKVLPEAYSVCCPRNTIEFYERYLEEYQRYLKPLVIKLEKGKVPSLSFVNEGQRRWA
                     RRDDAYYHELGNLYLSQAIELPRQMFDDEIKDKLREMPEMRDVDFDHANVTFLIGEYLK
                     RVRHDESQEFYSWPRHYKYVDMLKCILNPKNGSLQAVYIQMGEREGLWQERSELEEKYA
                     KIRLRDLGRKGLDKDEANERIKTGLGNRKKEYQKAEKVIRRYKVQDALLFMLAKNTLFN
                     SVEVDDERFKLKDIMPDGEKGILSEVVPMDFCFRSGNSATRKLMGTIHSDNTKIKNYGD
                     FFALANDKRMVTLLPLVGEQCLVKEEVKEEFDKYDDCRPEMISMVFDFEQWAYSAYPEL
                     KELVSNEAIKGRLFSNLLQELLGRGELTYEEKYALVGIANAFLANSYPKDGGVVKVRTL
                     PDIAKSLKDVFKEYIRLE"
     primer_bind     complement(6716..6729)
                     /label=Hgm6Cas13b-T-XbaI-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6847..6870)
                     /label=Hgm6Cas13b-T-XbaI-R
                     /note="color: #a020f0; direction: LEFT"
     CDS             6874..7590
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7618..7665
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7684..7749
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7756..8323
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             8343..8942
                     /codon_start=1
                     /label=PuroR
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8961..9549
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #a6acb3"
     CDS             complement(9432..9443)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     LTR             9624..9857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        10062..10166
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             10167..11027
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11198..11786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    12197..12218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        12233..12263
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
     protein_bind    12271..12287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     12295..12311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catggaggac
     3481 aacctgacaa aaaccaagaa gaagatgaga atccctaagc tgatcgagga gcacaagtcc
     3541 gtgttcgggg cctacagcac catggccctg agcaacgtgg agacagtgct gaaccacatc
     3601 gccgagaggg ccgggctgga cggctacgaa agagacaggg gccctggggt ggaggactac
     3661 tgggagcatc ccgtgatgca gtgcctctgc agaaaggaca agccaaggtc catcccttct
     3721 gacgtgctgc tggacgtgcg gaacaggctg ttcaggagct tcccatttct gaagatcatg
     3781 gctgagaacc agagggacta tagaaacgct aagggaaaag tggagtgcgt ggagatcaac
     3841 gaatccgaca ttttcgtggt gctcaataat agctttaggg tgctcaaagc ttacgccgat
     3901 acctgcaccg cctatctgat cgaaaaccgc atttgggaag ataattcccc aatgctgatg
     3961 tacaacgaat gccctctggc cgccatggtg aaccagtact acacagccgc cctgagggtg
     4021 actaaggaaa gatacgggta tgaaacaagg gacctgacct tcatccagaa gagaaggttt
     4081 aagcaggaac cagagaagga ggcttccggg aacgtgaaaa agaaggccgt gcctgacctg
     4141 gccttctttc tgagcctggt ggccctgaac ggggacggcc ggaagtggct gcacctgtcc
     4201 ggctggggag tggtgctgct gatttgcctg ttcctggaga agaagtacgt gaatgtgttt
     4261 ctgtctaagc tgcctaatcc aggcaattac ccacccagta gcaaagagcg gcgaattatc
     4321 aggagatcca tgggagtgtg cagcgtggtg ctgccaaaag agagaatcca ctctgagacc
     4381 ggggatctgt ctgtcgctct cgacatgctg aatgaactga agaggtgtcc cagagaactg
     4441 ttcgatacac tctcccccgg cgatcaggag cgcttcagaa ccattagcag cgatcacaac
     4501 gaagtgctcc agatgcggtc caaggacaga ttcgcacagc tggtgctgca atacatcgat
     4561 cataaccgcc tgttcgagaa cttgaggttc cacgtgaaca tgggcaagct gagatacctc
     4621 tttaatccta agaagtactg tatcgatggc cagaccaggg tgagagtgct ggagcacccg
     4681 ctcaatggat tcggaaggct tcaggagatg gaagagaaga gactgcagga gaacggacca
     4741 tttgcccggt ctggcatcaa ggtgcggtgc ttcgacgagg tgaggcggga cgacgctaac
     4801 gagtccaact acccttatat cgtggacact tacacacatt acgtgctgga aaacgacatg
     4861 gtggaaatgt tcttctgtcc cgaggggtcc gggatgaaga tgcctgaagt gaccagcagg
     4921 gagggcaagt ggtatgtcga caaaaaggtg cctcattgca gaatgagaat gtctgtgctg
     4981 gagctgcccg ccatgctgtt ccatctgctg ctgtgcggcg ccaagaacac cgaggtgcac
     5041 attggcaaag tgtgtgataa ctactgtcac ctgtttagcg acatggctca gggcaatctc
     5101 actgaggaga atattctgtc ctacggcatt aagaaggaag atattccaca gaaggtgtgg
     5161 gattgcgtgc gcggggtgca catgggaaag gactctcggg cctacaggga gaaagagatc
     5221 cgcgagcgct acgaggacgt gaccagacgg ctcgaaaggc tcgaggctga ccggaaggcc
     5281 gtgctcggcg gcgaaaacaa gatcgggaaa cgcggatttg tccagatcgt gcccggcaga
     5341 ctggccgcct acctcgcaac cgacatctgc aggctccagc ctagcctcag aaagggcgat
     5401 gggtacggaa cagatagact gaccggcctg aattttaggc tgctgcagtc ctcaattgcc
     5461 acatataatt gcggcgagag tgacatcctg tatggaagat ttcgcgacgt gttctgttct
     5521 gcaggcctca tcggaggcga caatccccat ccttttctgg ataaggtcct cccagaagcc
     5581 tatagcgtgt gttgccctcg caatacaatc gaattctatg agcgctacct ggaggagtac
     5641 cagcggtacc tgaagcctct tgtgatcaag ctcgaaaagg gcaaagtgcc tagcctgtcc
     5701 ttcgtgaatg agggccagag aaggtgggcc agaagggacg acgcctacta tcacgaactg
     5761 ggcaatctgt acctgagcca ggctatcgag ctgcccaggc agatgtttga cgatgagatt
     5821 aaggataagc tgagagaaat gccagagatg cgggacgtgg attttgatca cgccaacgtg
     5881 accttcctga tcggagagta tctgaaaagg gtccgccacg acgaaagtca ggagttctac
     5941 agctggcccc gccactataa gtacgtggac atgctgaaat gtatcctgaa tccaaagaac
     6001 ggatctctgc aggccgtgta tatccagatg ggggagcggg aaggcctgtg gcaggagcgg
     6061 agcgaactgg aagagaagta cgccaaaatc cggctgagag atctgggcag gaaggggctg
     6121 gacaaggatg aggctaacga aaggatcaag accggactcg gcaacagaaa gaaagagtac
     6181 cagaaggccg agaaagtgat ccggagatat aaggtgcagg acgcccttct gttcatgctg
     6241 gccaagaaca cactgtttaa ctccgtggag gtggatgatg aacgctttaa gctgaaggac
     6301 atcatgcctg acggcgagaa aggcatcctg tccgaggtgg tgcccatgga cttttgtttt
     6361 cggtctggga acagcgccac cagaaagctg atgggcacca tccacagcga taacaccaaa
     6421 attaagaact acggcgactt cttcgcactg gccaacgata agcggatggt gacactgctt
     6481 cccctggtgg gcgaacagtg tctggtgaaa gaggaggtga aagaagagtt cgataaatac
     6541 gatgactgca gacctgaaat gatcagtatg gtgtttgatt tcgagcagtg ggcctattct
     6601 gcctaccccg agctgaaaga gctggtgagc aatgaagcta ttaagggacg gctgttttca
     6661 aatctgctgc aggagctgct gggcagaggg gagctgacat acgaggaaaa gtacgcactg
     6721 gtgggaattg ccaacgcctt tctggccaat tcatacccta aggatggcgg agtggtgaag
     6781 gtgaggaccc tgcctgacat tgccaagtcc ctgaaggatg tgttcaagga gtacatccgc
     6841 ctggagagtc tagaggcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
     6901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     6961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     7021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     7081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     7141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     7201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     7261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     7321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     7381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     7441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     7501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     7561 atcactctcg gcatggacga gctgtacaag tcgaattcta agccaggatt ccatatgaag
     7621 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaacaacg cgtgcagcta
     7681 agcgactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
     7741 gatgacaagt aaggatccct cccccccccc taacgttact ggccgaagcc gcttggaata
     7801 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     7861 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     7921 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     7981 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     8041 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     8101 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     8161 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     8221 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     8281 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taaggatcca ccggagctta
     8341 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     8401 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     8461 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     8521 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     8581 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     8641 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     8701 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     8761 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     8821 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     8881 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     8941 gacgcccatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     9001 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     9061 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     9121 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     9181 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     9241 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     9301 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     9361 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     9421 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     9481 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     9541 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     9601 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     9661 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9721 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9781 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9841 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     9901 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     9961 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
    10021 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10081 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10141 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10201 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10261 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10321 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10381 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
    10441 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10501 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10561 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10621 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10681 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10741 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10801 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10861 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10921 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10981 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    11041 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    11101 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    11161 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11221 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11281 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11341 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11401 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11461 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11521 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11581 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11641 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11701 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11761 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11821 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11881 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    11941 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    12001 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
    12061 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    12121 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
    12181 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    12241 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    12301 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
    12361 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    12421 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    12481 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    12541 tttgttaaat atgtactaca aacttagtag t
//
