LOCUS       pHAGE-IRES-puro-       14339 bp DNA     circular SYN 29-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14339)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14339
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #a6acb3"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     CDS             3418..3465
                     /codon_start=1
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3475..3495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             3496..7278
                     /codon_start=1
                     /label=MisCas13b
                     /note="color: #993366"
                     /translation="FVRYSPESWPRHFHGLQQRLPTPIGQHTFLSDNFNTMDIRLSKNR
                     ALLAAYLNMAQQNVYVTLLHISQLMGVEETKDLSNRMYDFQILQELKTGKKGAESEQMV
                     KSERVQKIILLLYRNFPFLKAFVEAKYKQQEKNNGGKNKGGGKNTQKNKKTPDNTSNEE
                     QLEFTESELGKLPIPPHEYRTILCDVFRLINDLANEYTAFSPEKRTGGAKESHEKCQKK
                     MLSYLYLCLDGGRRVVKERFKDTFQEKDFNFLTQGPRYSLQKDGDKKKAEERPNFYYRL
                     KDDQGLFSYVGILLFISLFLHKRYISEMLAQSGLFYTPRYWKGQPVNVELERRIISEIF
                     SIYRIRLPKERIDSTRPDYALGLDMLNELQKCPNELYETFSPKDQAEFRVKREYDESGE
                     GTEEGEVLLKRFEDRFPYFAIRYLDENRVFDDIRFQVSIGKYRYKFYNKLCIDVVSSNR
                     NDDCYRLRSLQKEINGFGRLNEVEYERILRYGSHIHVEKPEGDDTPYLTDHHTSYLFNG
                     NRIGLLFNQENGRHDLEEIRGNCLYLPHLTFASSSEGKDARRVPPIAWLSTYELPALIF
                     HHLLCRHYGLERTATESIIKECVGLYHRLFTDIRDGKLLPGEALGIQAEESTRSEISRI
                     KKGEPLPQNISEKIETFLSTHYCTEQVCLHIQDIPKKIIDYLIGKNIDQDMSGLFKQRS
                     RERINKMYESTKIRLKKFKTDLDLQGNGDNKFGKKRYTDIRPGQLADYLAKDLLFFQPY
                     DDEKKNKVTGLNYQILQAALAIYNHNLDELRRLFKNARLLVPENPKMEHPFLGSVLHKS
                     PRTTLQFYAYYLLEKISYLKKRLDDPNETFADLFRYKQRQKWQTRDSQWYRALAGRYMA
                     EMMEEGTMNFKPVELPRGLFERPIKRLLTNILTKRTDSDSQALLKIVESPRCNVSYLIA
                     QYFTLILHDDSQPFYHPNKRYKRTYQYFNILNNRKERNKLVQVYYTLDEMEAYFKDTES
                     LKKRYKDYLTSSEAKKHPAPEQRLKKYLSEYRKNETTIRRYRVQDMLVFLMAKILLLEK
                     GGLEAGELEQYKLSHIQPTTGQNSGQNILSLQVPFNITVKLDDGTERSITQEVIKVKNY
                     GDFFRFLYDDRVASLLPYTQEDIPLERALLEKELEGYDRERIPIFKSVHCMEKEILRLC
                     PELNDPQSSQYKYTTDIGTEKIVKENFRAMLTELCSRGLLPENEIEMMVEIANSFSANR
                     YACVNLKGKNIPMIAISIQRKFDGVVGRTIGKNKNA"
     misc_feature    3925..4011
                     /label=2
                     /note="color: #a6acb3"
     CDS             4080..4109
                     /codon_start=1
                     /label=MisCas13b
                     /note="color: #993366"
                     /translation="PGQ*VHSLQP"
     primer_bind     complement(4822..4855)
                     /label=MisCas13b-K459A-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     4823..4855
                     /label=MisCas13b-K459A-F
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     4851..4886
                     /label=MisCas13b-R459A-F
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(4851..4886)
                     /label=MisCas13b-R459A-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4874..4912)
                     /label=MisCas13b-R467A-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     4875..4912
                     /label=MisCas13b-R467A-F
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(4879..4919)
                     /label=MisCas13b-L469A-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     4880..4919
                     /label=MisCas13b-L469A-F
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    5113..5154
                     /label=Feature 19
                     /note="color: #a6acb3"
     misc_feature    5353..5472
                     /label=3
                     /note="color: #a6acb3"
     misc_feature    6109..6138
                     /label=4
                     /note="color: #a6acb3"
     misc_feature    6220..6249
                     /label=5
                     /note="color: #a6acb3"
     misc_feature    6340..6387
                     /label=6
                     /note="color: #a6acb3"
     misc_feature    6904..7278
                     /label=Feature 22
                     /note="color: #a6acb3"
     misc_feature    7015..7047
                     /label=7
                     /note="color: #a6acb3"
     primer_bind     complement(7242..7253)
                     /label=MisCas13b-R459A-R
                     /note="color: #a020f0; direction: LEFT"
     CDS             7297..8010
                     /codon_start=1
                     /label=GFP
                     /note="color: #993366"
                     /translation="MRKGEELFTGAVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             8029..8742
                     /codon_start=1
                     /label=GFP
                     /note="color: #993366"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             8761..9474
                     /codon_start=1
                     /label=GFP
                     /note="color: #993366"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             9481..9501
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    9518..10091
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             10111..10710
                     /codon_start=1
                     /label=PuroR
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    10729..11317
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #a6acb3"
     CDS             complement(11200..11211)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     LTR             11392..11625
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        11830..11934
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             11935..12795
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12966..13554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    13965..13986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        14001..14031
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
     protein_bind    14039..14055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     14063..14079
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catgcccaaa
     3481 aagaagagga aagtgttcgt gagatatagc cctgagtcct ggcctaggca cttccacggc
     3541 ctgcagcaga gactgcccac ccccatcggc cagcacacct tcctgagtga taactttaac
     3601 accatggaca tccggctgag taagaataga gccctgctgg cagcttacct gaatatggcc
     3661 cagcagaacg tgtacgtgac cctgctgcac atctcccagc tgatgggagt ggaggagacc
     3721 aaagacctgt ctaaccgcat gtacgacttt cagattctgc aggagcttaa gaccgggaag
     3781 aagggcgccg agtctgagca gatggtgaag tctgagagag tgcagaagat cattctcctg
     3841 ctgtatcgca acttcccttt cctgaaggcc ttcgtggagg ctaagtacaa gcagcaggag
     3901 aagaacaatg ggggcaaaaa caagggcggc ggaaaaaaca cacagaagaa taagaaaacc
     3961 cctgacaata caagcaacga ggagcagctg gagttcactg agagtgagct gggcaagctg
     4021 cccattccac cacatgagta cagaaccatc ctgtgtgatg tgttcaggct gatcaatgac
     4081 ctggccaatg agtacacagc cttcagccct gagaaacgca ctggcggcgc taaggagtcc
     4141 cacgaaaagt gccagaagaa gatgctgagc tacctgtacc tgtgcctgga cggaggcaga
     4201 agggtggtga aggagcggtt caaggacacc ttccaagaga aggactttaa ctttctcacc
     4261 cagggcccaa gatacagcct ccagaaagac ggagataaga agaaggccga ggaaagacct
     4321 aatttttact acagactgaa ggatgaccag ggactgttct cttacgtcgg tatcctcctg
     4381 ttcatcagcc tgttcctgca caagaggtat atttccgaga tgctggccca gtccgggctg
     4441 ttttatacac ctcggtactg gaagggccag ccagtgaacg tggaactgga gaggaggatc
     4501 atcagtgaga tcttttccat ctatcgcatc aggctgccca aggagagaat cgactccacc
     4561 agaccagatt acgccctcgg cctggatatg ctgaatgagc tgcagaagtg ccctaatgag
     4621 ctgtatgaga ccttctcacc taaagaccag gctgagttta gagtgaagcg ggagtatgac
     4681 gagagcggcg aaggcacaga agagggcgag gtgctgctga aaagattcga ggacagattc
     4741 ccttatttcg ccattcgcta tctggacgag aatagagtgt tcgacgacat cagattccag
     4801 gtgagcatcg gcaagtatag gtacaaattc tacaataagc tgtgcatcga cgtggtgagc
     4861 agcaatcgga acgatgactg ctatcgcctg aggtccctgc agaaagagat taatggcttc
     4921 ggcagactga atgaagtgga gtacgagaga atcctgagat acgggtctca catccacgtg
     4981 gagaagcctg agggcgacga caccccctac ctgactgacc atcataccag ctacctgttt
     5041 aacggcaata ggatcggcct gctgttcaac caggagaacg gcaggcacga tctggaggag
     5101 atcagaggaa actgcctgta tctgcctcac ctgacttttg catcctcctc cgagggaaag
     5161 gatgccaggc gcgtgccccc catcgcctgg ctgtccacct atgagctgcc tgccctgatc
     5221 ttccaccacc tgctgtgcag acactatggc ctggagagaa ctgccaccga gagcatcatc
     5281 aaggaatgtg tgggcctgta ccaccggctg tttactgata tccgcgacgg taaactgctc
     5341 cccggagagg ccctgggcat ccaggccgag gagtccacaa ggagcgagat tagtaggatc
     5401 aagaagggtg aacccctgcc ccagaacatc tctgaaaaga ttgaaacctt cctgagcact
     5461 cactactgca ccgaacaggt gtgcctgcac atccaggaca ttcctaagaa aatcatcgat
     5521 tacctgatcg ggaaaaacat cgaccaggac atgagcggac tctttaagca gagaagcaga
     5581 gagcggatta ataagatgta tgagtccact aaaattcgcc tcaagaagtt caagaccgac
     5641 ctggacctgc agggcaacgg cgacaataag tttggaaaga agaggtacac tgacattagg
     5701 ccaggacagc tggccgacta tctggccaag gacctgctgt tcttccagcc atatgacgac
     5761 gagaagaaga ataaggtcac tggactgaac tatcagatcc tgcaggccgc actggccatc
     5821 tacaatcaca acctggatga gctcagacga ctcttcaaaa atgcccggct gctcgtgccc
     5881 gagaatccta agatggagca ccctttcctg ggctctgtgc tgcacaagtc cccccggacc
     5941 actctgcagt tctacgccta ctacctgctc gagaagatct cttacctgaa aaagagactc
     6001 gacgatccta acgagacatt cgccgatctg tttagatata agcagaggca gaaatggcag
     6061 actagagaca gccagtggta tagggccttg gctggcaggt acatggctga gatgatggaa
     6121 gagggcacca tgaattttaa acccgtggag ctgccaagag ggctgtttga aagacctatc
     6181 aagcggctgc tgaccaatat cctgactaag cggaccgaca gcgatagcca ggccctgctg
     6241 aagatcgtgg agagccccag gtgcaacgtg agctacctga ttgctcagta tttcactctg
     6301 attctgcacg acgattccca gcccttttac caccctaaca aacgctacaa aaggacctac
     6361 cagtatttca acatcctgaa caatagaaag gagcggaata agctcgtgca ggtgtactac
     6421 actctggatg agatggaggc ttactttaag gataccgaat ctctgaagaa gaggtataag
     6481 gactacctga ctagctccga ggctaagaag cacccagccc ccgagcagag gctgaagaag
     6541 tatctgtcag agtatcgcaa gaatgagacc accattagac ggtacagagt gcaggacatg
     6601 ctggtgtttc tgatggccaa aattctgctg ctggaaaagg gcggcctcga agccggcgag
     6661 ctggagcagt acaagctgtc ccacattcag cccaccaccg gccagaacag cggccagaac
     6721 atcctgagcc tgcaggtgcc ctttaacatc accgtgaagc tggacgatgg cacagagaga
     6781 agcatcaccc aggaagtgat caaggtgaaa aattatggcg acttctttag atttctctat
     6841 gatgaccggg tggcatccct gctgccttat actcaggagg acattcccct ggagcgggcc
     6901 ctgctggaga aggagctgga gggatacgac agggagagaa tccctatttt caagagcgtg
     6961 cactgcatgg agaaggagat cctgaggctg tgccctgaac tgaatgatcc ccagtccagc
     7021 cagtacaagt ataccacaga tattggcact gagaagatcg tgaaggagaa tttcagggcc
     7081 atgctgactg agctgtgtag ccggggcctg ctccctgaga acgagatcga gatgatggtg
     7141 gagatcgcca atagcttttc cgccaataga tacgcctgcg tgaacctgaa gggcaagaat
     7201 atcccaatga tcgccatctc catccagcgg aagttcgacg gcgtggtggg cagaaccatc
     7261 ggaaagaata agaacgcggg aggtggatcc ggaagtatgc gtaaaggcga agagctgttc
     7321 actggtgccg tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc
     7381 gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt
     7441 actactggta aactgccggt accttggccg actctggtaa cgacgctgac ttatggtgtt
     7501 cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg
     7561 ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg
     7621 cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt
     7681 gactttaaag aagacggcaa tatcctgggc cataagctgg aatacaattt taacagccac
     7741 aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc
     7801 cacaacgtgg aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc
     7861 ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct
     7921 aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc
     7981 atcacgcatg gtatggatga actgtacaaa tctggcggcc gctctggcat gcgtaaaggc
     8041 gaagagctgt tcactggtgt cgtccctatt ctggtggaac tggatggtga tgtcaacggt
     8101 cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa actgacgctg
     8161 aagttcatct gtactactgg taaactgccg gtaccttggc cgactctggt aacgacgctg
     8221 acttatggtg ttcagtgctt tgctcgttat ccggaccata tgaagcagca tgacttcttc
     8281 aagtccgcca tgccggaagg ctatgtgcag gaacgcacga tttcctttaa ggatgacggc
     8341 acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa ccgcattgag
     8401 ctgaaaggca ttgactttaa agaagacggc aatatcctgg gccataagct ggaatacaat
     8461 tttaacagcc acaatgttta catcaccgcc gataaacaaa aaaatggcat taaagcgaat
     8521 tttaaaattc gccacaacgt ggaggatggc agcgtgcagc tggctgatca ctaccagcaa
     8581 aacactccaa tcggtgatgg tcctgttctg ctgccagaca atcactatct gagcacgcaa
     8641 agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata tggttctgct ggagttcgta
     8701 accgcagcgg gcatcacgca tggtatggat gaactgtaca aaggtagcat cgatggtagc
     8761 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
     8821 gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt
     8881 aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg
     8941 gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag
     9001 catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt
     9061 aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta
     9121 aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag
     9181 ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc
     9241 attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat
     9301 cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat
     9361 ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg
     9421 ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaactcgag
     9481 ccaaagaaaa agcggaaagt gtaatctaga ggatctgccc ctctccctcc ccccccccta
     9541 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     9601 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     9661 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     9721 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     9781 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     9841 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     9901 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     9961 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
    10021 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
    10081 cacgatgata aggatccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc
    10141 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
    10201 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
    10261 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
    10321 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
    10381 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
    10441 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
    10501 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
    10561 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
    10621 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
    10681 tggtgcatga cccgcaagcc cggtgcctga cgcccatcga tagatcctaa tcaacctctg
    10741 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
    10801 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    10861 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    10921 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    10981 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    11041 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    11101 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    11161 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    11221 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    11281 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
    11341 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    11401 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
    11461 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    11521 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    11581 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
    11641 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
    11701 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
    11761 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
    11821 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    11881 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    11941 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    12001 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    12061 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    12121 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
    12181 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    12241 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    12301 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    12361 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    12421 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    12481 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    12541 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    12601 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    12661 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    12721 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    12781 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    12841 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    12901 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    12961 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    13021 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    13081 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    13141 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    13201 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    13261 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    13321 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    13381 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    13441 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    13501 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    13561 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    13621 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    13681 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    13741 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
    13801 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    13861 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
    13921 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    13981 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    14041 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
    14101 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    14161 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    14221 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    14281 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
