LOCUS       pHAGE-IRES-puro-       13937 bp DNA     circular SYN 17-JUL-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13937)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13937
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #a6acb3"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff"
     promoter        2242..2453
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
                     /note="color: #ffffff"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     3184..3307
                     /label=Fragment.FOR
                     /note="color: #a020f0; direction: RIGHT"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3475..3495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             3496..6867
                     /codon_start=1
                     /label=Hgm4Cas13b
                     /note="color: #993366"
                     /translation="MKIPQIIENNKHLFGAYSTMALSNIRNILDHIATLADIENDFYSD
                     SDSYWYHPCIKIIDPRNNCTDAAKADFVIEKIKSHFPFVPIVAEAQRQSDILRAKKQLD
                     IAVRNRDYQKQEELKHRQKSLLSVTNADIYRVLNNMLWVLANYANQTVAYLISDIKFED
                     GSDFLNYNEQPLSRMLNNYFTIALRDTANKYSYSPEALSFIQSSRYKTENRRKILDTDF
                     FLSIQHNNSDTNPKNLHLSGVGVALLICLFLEKKYVNVFLQKLPIYGPYKKQSMEANII
                     RQSFSIHTVKLPKERIVSEKSDFTIALDMLNELKRCPKQLFSTLSYADQNAFRIVSSDM
                     SDVLQVRHTDRFAQLSLEYIDRRELFSDIRFHLNMGKLRYLKTADKHCIDGVSRVRVLE
                     GKINAFGRIHEFEARRKEQGFVEWHEQAARLIKTNKNIEIRDFEHVKRDDATPDSYPYI
                     IDTYTHYLLENNKIEMRFGDYLPKLVKVDDQKWTVYNEMPTCCMSALELPAMMFHMMLC
                     GSEATEHLIKAEVEKYKKLFGAMANGTLSKENIGNFEIAMEDLPKKVIDCVNRKKSGKS
                     FVKQIKKEIAEMLADTNLRIVRLKSDKRSVASTQNKMGKRGFRSIQPGKLADWLAADIV
                     KHQPSLFSGDDYGTDRITGMNYRVMQSTIATFNAATPEHSLDELKRVFSAAQLIQCQER
                     EHPFLQKALNRNPQNTIDFYEFYLQAKRAYYQSLRQSIENGEDVKLPYLNPDRNKWVRR
                     GASFYSTMGDIYLEDMPIELPRQMFDKEIKKALAQLPSMKGVDMKQSNVTFLIAEYLKR
                     ELKDDFQPFYQWNRNYRFTDMMICEENCDTKALASKFIPLELREEIWEKRGELKDAYKR
                     WAFGRLNKNPDIKRLSPAEKDELLEKRIAKCRNEYQKSEKIIRRYKVQDVLMFMMVKSM
                     FAQGVFTAESKEFALSTITPDAERGILSEVVPIDFKFSIDGKTYTIHSNGMKIKNYGDF
                     YKLINDKRMKSLLNIVTHSTIEKDLLEKEFSTYDDKRPEAVEIIFEFEKAAYEKHPELK
                     ELLTPERKFDFRALLEELQAKSDLSQIDARYLSQIANAFNANAYPRNLKITSNIPEIAQ
                     EMVNIFRITDPYKKGKK"
     misc_feature    3754..3870
                     /label=LDKKEVPIKE
                     /note="color: #a6acb3"
     misc_feature    4117..4155
                     /label=D6
                     /note="color: #a6acb3"
     primer_bind     complement(4626..4664)
                     /label=Hgm4Cas13b-K386A-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     4634..4680
                     /label=Hgm4Cas13b-K386A-F
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(4654..4692)
                     /label=Hgm4Cas13b-R396A-R
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     4662..4704
                     /label=Hgm4Cas13b-R396A-F
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    4780..4866
                     /label=D7
                     /note="color: #a6acb3"
     misc_feature    5086..5145
                     /label=D8
                     /note="color: #a6acb3"
     primer_bind     complement(5371..5392)
                     /label=Mhd-R191-R
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    5962..6216
                     /label=QTENYPLVELEKKQADR
                     /note="color: #a6acb3"
     misc_feature    6301..6372
                     /label=D4
                     /note="color: #a6acb3"
     misc_feature    6373..6519
                     /label=D3
                     /note="color: #a6acb3"
     misc_feature    6520..6624
                     /label=D2
                     /note="color: #a6acb3"
     misc_feature    6625..6867
                     /label=D1
                     /note="color: #a6acb3"
     primer_bind     complement(6855..6875)
                     /label=Hgm4-SNAPf-R
                     /note="color: #a020f0; direction: LEFT"
     CDS             6895..7608
                     /codon_start=1
                     /label=GFP
                     /note="color: #993366"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             7627..8340
                     /codon_start=1
                     /label=GFP
                     /note="color: #993366"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             8359..9072
                     /codon_start=1
                     /label=GFP
                     /note="color: #993366"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             9079..9099
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    9122..9689
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             9709..10308
                     /codon_start=1
                     /label=PuroR
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    10327..10915
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #a6acb3"
     CDS             complement(10798..10809)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     LTR             10990..11223
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        11428..11532
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             11533..12393
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12564..13152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    13563..13584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        13599..13629
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
     protein_bind    13637..13653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     13661..13677
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catgcccaaa
     3481 aagaagagga aagtgatgaa gatcccccag atcatcgaga acaacaagca cctgttcggc
     3541 gcctacagca ctatggccct gagcaacatt cgcaacatcc tggatcacat cgccaccctg
     3601 gctgacatcg aaaatgactt ctacagcgat agcgactctt attggtacca cccatgcatc
     3661 aagatcatcg accctcggaa caactgcacc gacgccgcca aggctgattt cgtgatcgag
     3721 aagattaagt ctcatttccc attcgtgcca atcgtggccg aggcccagag gcagtccgat
     3781 atcctgagag ccaagaagca gctggacatc gccgtgagaa atagggatta ccagaagcag
     3841 gaggagctga agcaccggca gaagtccctg ctgtccgtga ccaatgccga tatctaccgg
     3901 gtgctgaata acatgctgtg ggtgctggcc aactacgcca accagaccgt ggcctacctg
     3961 atcagcgaca tcaagttcga agatggttcc gactttctca attataacga gcagcctctg
     4021 tcccggatgc tgaataacta cttcaccatc gccctccggg acaccgctaa caagtacagc
     4081 tatagccctg aggccctgag cttcatccag tcaagtaggt ataagactga gaatcggcgg
     4141 aaaatcctgg acacagactt tttcctgtct atccagcaca acaacagcga tactaaccct
     4201 aagaaccttc acctgtccgg cgtgggcgtg gccctgctga tttgtctgtt tctggagaaa
     4261 aaatatgtga atgtgtttct gcagaagctg cccatctacg gaccctacaa gaagcagtcc
     4321 atggaggcca acatcatcag acagagcttt tccatccaca ccgtgaagct gccaaaggag
     4381 cggattgtct ctgaaaagag cgacttcacc attgcactgg atatgctgaa cgagctgaag
     4441 agatgcccca agcagttgtt tagcaccctg tcctacgctg accagaacgc cttcagaatc
     4501 gtgagcagtg atatgagcga cgtgctgcag gtgaggcata ctgaccggtt cgcccagtta
     4561 agcctggagt acatcgatcg gcgggaactg tttagcgaca ttagatttca cctgaacatg
     4621 ggcaagctgc ggtacctgaa aaccgctgac aagcactgca tcgatggtgt gtctagggtg
     4681 agagtgctgg agggtaaaat taacgccttt ggccgcattc atgagtttga ggccaggcgg
     4741 aaggagcagg gattcgtgga gtggcacgag caggcagccc ggctgatcaa gaccaataag
     4801 aatatcgaaa tcagggactt tgagcacgtg aagcgcgacg acgccacacc cgatagctac
     4861 ccctacatta ttgacactta cacccactat ctgctggaga ataataagat cgagatgagg
     4921 tttggcgact acctcccaaa actggtgaag gtggacgacc agaagtggac cgtgtacaac
     4981 gaaatgccca catgttgcat gtccgccctg gagctgcccg ccatgatgtt ccacatgatg
     5041 ctgtgcggca gcgaagcaac agagcatctg atcaaggccg aggtggagaa gtataagaag
     5101 ctctttggcg ccatggctaa cggcacactg tctaaggaga atatcggcaa cttcgagatt
     5161 gccatggagg acctgccaaa gaaagtgatc gactgcgtga ataggaagaa gtctgggaag
     5221 tcttttgtca agcagattaa gaaggagatc gccgagatgc tggccgacac caacctgagg
     5281 attgtgagac tgaagtccga taagcgcagc gtggcctcca cccagaataa gatgggcaaa
     5341 cggggcttta gaagcattca gcccggcaag ctggccgact ggctcgccgc cgatatcgtg
     5401 aaacaccagc ctagcctgtt tagcggcgac gactacggta ccgataggat taccggcatg
     5461 aattaccggg tcatgcagtc tactatcgct accttcaatg ctgccacccc cgagcacagc
     5521 ctggacgagc tgaaaagagt gtttagcgcc gcacagctga ttcaatgtca ggagagggag
     5581 cacccttttc tccagaaggc cctgaatcgc aatccccaga acaccatcga cttctacgag
     5641 ttctatctgc aggctaagag agcttactat cagtccctgc ggcagtctat cgagaacggc
     5701 gaggacgtga aactgccata cctgaaccct gatcgcaata agtgggtgag gagaggggcc
     5761 agcttttaca gcaccatggg agatatctac ctggaggaca tgcccattga gctgccaagg
     5821 cagatgttcg acaaggagat caagaaggcc ctggcccagc tgccttccat gaagggcgtg
     5881 gatatgaagc agagcaacgt gaccttcctg atcgccgagt acctcaagag agaactgaag
     5941 gatgactttc agccatttta tcagtggaac agaaattaca ggtttacaga catgatgatt
     6001 tgcgaggaga actgcgacac caaggccctg gcatccaaat tcatccctct ggagctccgc
     6061 gaggagatct gggagaagcg gggcgagctg aaagatgcct acaaaagatg ggctttcggc
     6121 agactgaata agaatcctga tatcaagcgg ctgagccctg ccgagaagga cgaactgctg
     6181 gaaaagcgca tcgccaagtg tcgcaacgag taccagaagt ccgagaagat cattcggaga
     6241 tacaaggtgc aggatgtcct gatgttcatg atggtcaagt ccatgtttgc tcagggagtg
     6301 ttcaccgccg agtccaagga gtttgccctg tccacaatca cccctgacgc cgagcggggc
     6361 attctgtccg aagtggtgcc catcgatttc aagttttcca tcgacggaaa gacctacacc
     6421 atccactcca acggcatgaa gatcaagaac tacggcgatt tttacaagct gatcaacgac
     6481 aagagaatga agtctctgct gaatatcgtg acccactcta ctatcgagaa ggacctgctg
     6541 gagaaggagt ttagcaccta cgacgataag agacctgagg ccgtggagat catcttcgag
     6601 tttgagaagg ccgcctacga gaagcacccc gagctgaagg agctgctgac tcccgagcgc
     6661 aagtttgatt tcagggccct gctggaggag ctccaggcca agtctgacct gtctcagatc
     6721 gatgccagat atctgagcca gatcgccaac gccttcaacg ccaacgccta cccccggaat
     6781 ctgaagatca catccaacat ccctgagatc gcccaggaga tggtgaacat cttcaggatc
     6841 accgacccct acaagaaagg caagaagagt ctagagggag gtggatccgg aagtatgcgt
     6901 aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc
     6961 aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg
     7021 acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac tctggtaacg
     7081 acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac
     7141 ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat
     7201 gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc
     7261 attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca taagctggaa
     7321 tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa
     7381 gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac
     7441 cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc
     7501 acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag
     7561 ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaatc tggcggccgc
     7621 tctggcatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg
     7681 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact
     7741 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg
     7801 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg
     7861 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt
     7921 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc
     7981 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc
     8041 cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa
     8101 aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcagctg
     8161 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat
     8221 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg
     8281 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa
     8341 ggtagcatcg atggtagcat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt
     8401 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
     8461 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
     8521 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
     8581 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
     8641 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
     8701 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc
     8761 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
     8821 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
     8881 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
     8941 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
     9001 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
     9061 gaactgtaca aactcgagcc aaagaaaaag cggaaagtgt aatctagagg atctgcccct
     9121 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     9181 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     9241 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     9301 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     9361 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     9421 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     9481 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     9541 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     9601 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     9661 tggttttcct ttgaaaaaca cgatgataag gatccaccgg agcttaccat gaccgagtac
     9721 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     9781 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     9841 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     9901 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     9961 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    10021 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    10081 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    10141 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
    10201 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    10261 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccatcgata
    10321 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    10381 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    10441 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    10501 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    10561 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    10621 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    10681 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    10741 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    10801 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    10861 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
    10921 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    10981 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    11041 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    11101 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    11161 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    11221 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
    11281 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
    11341 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    11401 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    11461 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    11521 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    11581 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    11641 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    11701 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    11761 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    11821 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    11881 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    11941 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    12001 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    12061 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    12121 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    12181 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    12241 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    12301 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    12361 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    12421 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    12481 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    12541 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    12601 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    12661 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    12721 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    12781 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    12841 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    12901 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    12961 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    13021 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    13081 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    13141 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    13201 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    13261 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    13321 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    13381 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    13441 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    13501 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    13561 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    13621 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    13681 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    13741 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    13801 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    13861 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    13921 actacaaact tagtagt
//
