LOCUS       pHAGE-IRES-puro-       14357 bp DNA     circular SYN 18-JUL-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14357)
  AUTHORS   ylz
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14357
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #a6acb3"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     CDS             3418..3465
                     /codon_start=1
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     3447..3518
                     /label=CQ31
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     3450..3471
                     /label=dPba3Cas13b-5'T-F1
                     /note="color: #a020f0; direction: RIGHT"
     CDS             3475..3495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             3496..7296
                     /codon_start=1
                     /label=Pba3Cas13b
                     /note="color: #993366"
                     /translation="MAFDKPRPKRRQARSDFYITDKAIMGGYFNLAQLNFFKTLMEIFT
                     KAGIDVSKIKQDNSPKYLMILIKKLTHDDEKIDDKKWADALDLSNECLLKLQQLLYKHF
                     PFLGPVMGGEASYNIYKLKDGHPEVKVANDVMRGMKLEDCLAVLRHFALCLNDCANFYT
                     AFNPYNSIDAQKEQYLSQNKVAVWLEKVKDASRRIVKQNNQLTSQDMEFLTGIDHMKPQ
                     DKVDDFGNIMTDKKGYTIKEYVEYEDYYFRIRGKRYLVDAAGAKLADQEPRNALSDFGV
                     VFLCTLFLENDQSRRMLDELRLFETGPYDGSRDGDEFKNDILREILLFYRARIPRGKRL
                     DPMDDTTLLAMDMLNELRKCPMPLYDVLSREGQRFFEDEVKHPNDLTPEVAKRLRSSDR
                     FPYLALRYIDLNKCFDNIRFQVQLGKFRYKFYDKTTIDGEQVVRGLQKEVNAYGRFQDV
                     EHYRQEKYADMLQQTELVETGEEDITIANFIPDTPQSSPYLSDRTASYNIHNNRIGLLW
                     NMPGEHEVLTGDEKMYLPDLNVDDNGKADVFLPAPKASLSVRDLPALVFYLYLQNQHPD
                     LTPAEAIIQQKYNALVRFFEDVSTGRLLPVKGIAELEAAIDKYGHLTVHEIPEKLRDYL
                     AGVAGTEDDDDCADRLDCYAMGILEKRYRQVAARIDQLKEARKMVGDKMNRYGKKSYID
                     VRPRRLAEQLAQSIVEWQPATDNGRHKMTGKNFQRLIAFLARYIDEVPLQELKDLMTRA
                     RLLEGPVAHPFLQQVLDKNPLNIEVLYDLYFKEEKRYLDKYIDIEINKQGEEVYWLKAD
                     ADLSKIPFLNSSRLRFHKRDSASFQRLAARYLQVDGRQSTILLPDGLFTPHILSALKST
                     LYQENHDLQDHLQDQELNQNASYLMSVYLEHVLHDSAQPFYLSNSNDSPFARRYDLFAI
                     VQNKKRGAELLPGPLTPVEINRRLTAKTPDGSAKLIERDIVALLEDMARKTEQRIHKLP
                     QDLYLKKKKQAQEERIKLQRRLKHKITDVKKNERAIRRYKTQDMAIFLMAQQMLANQLS
                     WQNQEGEVPLKLANVCNDGFLSQTMKVSFPYEVPLKEDKTKKVTIDIVQDNMSLKNYGL
                     LYKLYHDERVVTLLQNIVDQQQISFAELTAELSLYDQNRSKAFSNFQQFEKLAFETNRE
                     ALSNPEHRGFFLEKVNKKNQVIRYERRNNFDALLDLLDAIEAHQLDPRDRQWLTSVANA
                     FCANSYYKIDMQQIEKKLPTIIQQIVEIVQRITDKASHPSNR"
     primer_bind     3562..3581
                     /label=dPba3Cas13b-5'T-F1
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(3950..3989)
                     /label=CQ32
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     3967..4013
                     /label=CQ33
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    3973..3984
                     /label=motif 1
                     /note="color: #a6acb3"
     primer_bind     complement(6101..6121)
                     /label=Pba3Cas13b-3'T-R6
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6185..6204)
                     /label=Pba3Cas13b-3'T-R5
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6473..6492)
                     /label=dPba3Cas13b-3'T-R4
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6644..6663)
                     /label=Pba3Cas13b-3'T-R3
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6698..6717)
                     /label=dPba3Cas13b-3'T-R2
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6971..6990)
                     /label=Pba3-3'T-R1
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(7150..7181)
                     /label=CQ34
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     7159..7198
                     /label=CQ35
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    7164..7176
                     /label=motif 2
                     /note="color: #a6acb3"
     primer_bind     complement(7278..7296)
                     /label=CQ36
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(7296..7311)
                     /label=Pba3-3'T-R1
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(7296..7311)
                     /label=Pba3Cas13b-3'T-R3
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(7296..7311)
                     /label=dPba3Cas13b-3'T-R4
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(7296..7311)
                     /label=dPba3Cas13b-3'T-R2
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(7296..7311)
                     /label=Pba3Cas13b-3'T-R5
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(7296..7311)
                     /label=Pba3Cas13b-3'T-R6
                     /note="color: #a020f0; direction: LEFT"
     CDS             7315..8028
                     /codon_start=1
                     /label=GFP
                     /note="color: #993366"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             8047..8760
                     /codon_start=1
                     /label=GFP
                     /note="color: #993366"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             8779..9492
                     /codon_start=1
                     /label=GFP
                     /note="color: #993366"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             9499..9519
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    9536..10109
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             10129..10728
                     /codon_start=1
                     /label=PuroR
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    10747..11335
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #a6acb3"
     CDS             complement(11218..11229)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     LTR             11410..11643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        11848..11952
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             11953..12813
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12984..13572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    13983..14004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        14019..14049
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
     protein_bind    14057..14073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     14081..14097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catgcccaaa
     3481 aagaagagga aagtgatggc ctttgataag cctagaccca aacggaggca ggccaggtct
     3541 gatttttaca tcactgacaa ggctatcatg gggggctact ttaatctggc ccagctgaat
     3601 ttctttaaga ccctgatgga gatcttcaca aaagctggca tcgatgtgag caaaatcaag
     3661 caggataaca gccctaagta cctgatgatt ttgattaaga aactgaccca tgatgatgaa
     3721 aagattgatg acaagaagtg ggccgatgcc ctggatctga gcaacgaatg tctcctgaag
     3781 ctgcagcagc tgctctataa acatttcccc ttcctgggac cagtgatggg cggagaggct
     3841 tcctacaata tctacaagct gaaggacggc caccctgaag tgaaggtggc caatgacgtc
     3901 atgagaggca tgaagctgga ggactgcctg gctgtgctga ggcatttcgc actctgcctg
     3961 aatgactgtg ccaactttta tacagccttt aacccatata actctattga cgcccagaag
     4021 gagcagtatt tgagccaaaa taaggtggct gtttggctgg aaaaggtgaa ggatgcaagc
     4081 aggagaatcg tgaagcagaa caatcagctg acctctcagg acatggaatt tctgaccggc
     4141 atcgaccaca tgaagcccca ggacaaggtg gatgacttcg gcaacatcat gaccgacaag
     4201 aaaggctaca ccattaagga gtacgtggag tatgaggatt attattttcg catcagggga
     4261 aaacgctatc tggtggatgc agccggcgcc aaactggccg atcaggaacc acgaaatgcc
     4321 ctctctgact tcggcgtggt gttcctgtgc accctgtttc tcgaaaatga tcagagcaga
     4381 agaatgctcg acgagctgag actgttcgag actggtcctt acgacggcag cagggacgga
     4441 gatgagttca agaacgacat cctgagggag attctgctgt tctacagagc ccgcatcccc
     4501 cgcggaaaga ggcttgatcc tatggatgac actacactgc tcgccatgga catgctcaac
     4561 gagctgagaa agtgcccaat gcctctgtac gacgtgctgt ctagggaggg ccagcgcttc
     4621 ttcgaggacg aggtgaaaca ccctaacgac ctcactcccg aggttgccaa gcgcctcagg
     4681 agttccgata ggttcccata cctggccctg cgatacattg atctgaataa gtgtttcgat
     4741 aacatccgct tccaggtgca gttagggaag ttcaggtaca aattttacga taagaccact
     4801 attgacggtg agcaggtggt tagaggcctc cagaaggaag tgaacgccta cggccggttc
     4861 caggatgtgg agcactacag acaggaaaag tatgcagata tgctgcagca gactgagctg
     4921 gtggaaactg gcgaggagga tatcaccatt gccaatttca tccccgatac tccccagtct
     4981 agcccctacc tgagtgatag gaccgctagt tacaatattc acaacaaccg catcggcctc
     5041 ctttggaaca tgcccggaga gcacgaagtt ctgaccggcg atgagaaaat gtacctgcct
     5101 gacctgaatg tggacgacaa cggcaaggcc gatgtgttcc tgcccgcccc taaagcctct
     5161 ctgtccgtca gggacctgcc cgctcttgtg ttttatctgt atctccaaaa tcagcacccc
     5221 gacctgacac cagctgaggc cattatccag cagaagtata atgccctggt gagatttttc
     5281 gaggatgtca gcacaggcag gctcctgccc gtgaaaggaa ttgctgagct ggaggccgcc
     5341 atcgacaagt atggacacct gaccgtgcac gaaattccag aaaagctgcg cgactacctc
     5401 gccggagtgg ccggaactga agatgacgac gactgcgccg atcgacttga ctgctacgca
     5461 atgggcattc tggaaaagag atatagacag gtggccgcca ggatcgacca gctgaaggag
     5521 gccagaaaga tggttggcga caaaatgaac aggtatggca aaaagtccta catcgacgtg
     5581 agacccagga gactggcaga gcagctggca cagtctatcg tggagtggca gccagccact
     5641 gataatggac gccacaaaat gaccgggaag aacttccagc ggctgatcgc ctttctggct
     5701 cgctatatcg acgaggtgcc tctccaggag ctgaaagacc tgatgacaag ggcccgcctg
     5761 ctggaggggc cagtggccca tccctttctg cagcaagtgt tggacaagaa tcccctgaac
     5821 atcgaggtgc tgtatgatct ctattttaaa gaggaaaaaa gatacctgga caagtacatc
     5881 gatattgaga tcaacaaaca gggcgaggag gtatattggc tgaaggctga cgccgatctg
     5941 tctaaaatcc ccttcctgaa tagctctagg ctgaggttcc acaaacggga tagcgccagc
     6001 ttccagcgcc ttgccgccag gtacctccag gtggacggac gccagtccac tattctgctc
     6061 ccagatggac ttttcacccc tcatattctg agtgccctga agtccactct gtaccaggaa
     6121 aaccatgacc tgcaggatca ccttcaggat caggagctga accagaatgc ctcttatctt
     6181 atgtccgtgt atctggagca tgtgctgcat gactctgccc agccttttta cctgtcaaac
     6241 agcaatgatt cccccttcgc ccggagatat gatctcttcg ctatcgtgca gaataagaag
     6301 agaggcgctg aactgcttcc aggccctctg accccagtgg agatcaatag gcgcctgaca
     6361 gctaagacac cagacggctc cgcaaagctc attgaacgtg acatcgttgc cctcctggag
     6421 gacatggccc gcaagaccga gcagagaatt cacaagctgc cccaggatct ctatctgaaa
     6481 aagaagaagc aggcccagga ggagcggatt aagctgcagc gccgcctgaa acacaagatc
     6541 acagatgtga agaagaacga gcgcgccatt cggagataca aaactcagga tatggcaatc
     6601 ttcctgatgg cccagcagat gctcgccaac caactgtctt ggcagaatca ggaaggagag
     6661 gtgcccctga agctggccaa cgtgtgcaat gacggcttcc tgagccagac catgaaggtg
     6721 agctttcctt acgaagtgcc actgaaagag gacaagacta agaaagtgac aattgatatt
     6781 gtgcaggaca atatgtccct gaagaactac ggcctgctgt ataagctgta ccacgacgag
     6841 cgcgtggtga cactgctgca gaacatcgtg gaccagcagc agatttcttt cgccgagctg
     6901 actgcggagc tgagcctcta tgaccagaac aggagcaagg ccttctccaa ctttcagcag
     6961 tttgagaaac tggcctttga aaccaatagg gaagcactgt caaaccccga gcatagagga
     7021 ttcttcctcg aaaaggtgaa taagaaaaac caggtcatca ggtacgagcg gaggaacaat
     7081 tttgatgctc tcctggacct gctggatgcc atcgaggccc accagctgga ccctcgcgat
     7141 agacagtggc tgacatctgt ggccaacgcc ttttgtgcca acagctatta caagattgac
     7201 atgcagcaga tcgagaaaaa gctgcctacc atcatccagc aaatcgtgga aattgtgcag
     7261 aggatcacag ataaagccag ccaccccagc aataggggag gtggatccgg aagtatgcgt
     7321 aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc
     7381 aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg
     7441 acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac tctggtaacg
     7501 acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac
     7561 ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat
     7621 gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc
     7681 attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca taagctggaa
     7741 tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa
     7801 gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac
     7861 cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc
     7921 acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag
     7981 ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaatc tggcggccgc
     8041 tctggcatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg
     8101 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact
     8161 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg
     8221 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg
     8281 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt
     8341 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc
     8401 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc
     8461 cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa
     8521 aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcagctg
     8581 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat
     8641 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg
     8701 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa
     8761 ggtagcatcg atggtagcat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt
     8821 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
     8881 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
     8941 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
     9001 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
     9061 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
     9121 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc
     9181 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
     9241 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
     9301 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
     9361 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
     9421 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
     9481 gaactgtaca aactcgagcc aaagaaaaag cggaaagtgt aatctagagg atctgcccct
     9541 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     9601 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     9661 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     9721 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     9781 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     9841 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     9901 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     9961 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
    10021 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
    10081 tggttttcct ttgaaaaaca cgatgataag gatccaccgg agcttaccat gaccgagtac
    10141 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    10201 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
    10261 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    10321 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    10381 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    10441 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    10501 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    10561 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
    10621 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    10681 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccatcgata
    10741 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    10801 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    10861 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    10921 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    10981 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    11041 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    11101 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    11161 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    11221 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    11281 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
    11341 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    11401 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    11461 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    11521 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    11581 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    11641 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
    11701 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
    11761 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    11821 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    11881 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    11941 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    12001 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    12061 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    12121 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    12181 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    12241 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    12301 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    12361 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    12421 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    12481 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    12541 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    12601 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    12661 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    12721 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    12781 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    12841 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    12901 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    12961 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    13021 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    13081 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    13141 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    13201 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    13261 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    13321 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    13381 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    13441 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    13501 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    13561 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    13621 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    13681 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    13741 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    13801 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    13861 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    13921 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    13981 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    14041 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    14101 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    14161 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    14221 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    14281 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    14341 actacaaact tagtagt
//
