LOCUS       pJH2999                 7370 bp    DNA        circular     30-AUG-2022
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             2845..2901
                     /locus_tag="G-CaMP5i"
                     /label="G-CaMP5i"
                     /ApEinfo_label="G-CaMP5i"
                     /ApEinfo_fwdcolor="#43ff00"
                     /ApEinfo_revcolor="#43ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1400..1574
                     /note="calcium indicator; contains permutated EGFP"
                     /codon_start=1
                     /transl_table=11
                     /product="GCaMP3"
                     /protein_id="ADJ53338.1"
                     /db_xref="GI:299818413"
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWN
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGD
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFR
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
                     "
                     /locus_tag="GCaMP3"
                     /label="GCaMP3"
                     /ApEinfo_label="GCaMP3"
                     /ApEinfo_fwdcolor="#ffb7b4"
                     /ApEinfo_revcolor="#ffb7b4"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2845..2901
                     /locus_tag="CaM domain"
                     /label="CaM domain"
                     /ApEinfo_label="CaM domain"
                     /ApEinfo_fwdcolor="#ffbc2f"
                     /ApEinfo_revcolor="#ffbc2f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1969..2403
                     /locus_tag="GFP 1-144"
                     /label="GFP 1-144"
                     /ApEinfo_label="GFP 1-144"
                     /ApEinfo_fwdcolor="#64ff00"
                     /ApEinfo_revcolor="#64ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(1400..1579,1630..1912,1964..2425,2477..2844)
                     /locus_tag="G-CaMP3i"
                     /label="G-CaMP3i"
                     /ApEinfo_label="G-CaMP3i"
                     /ApEinfo_fwdcolor="#00ff13"
                     /ApEinfo_revcolor="#00ff13"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    1511..1573
                     /locus_tag="M13"
                     /label="M13"
                     /ApEinfo_label="M13"
                     /ApEinfo_fwdcolor="#ff65ca"
                     /ApEinfo_revcolor="#ff65ca"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          3838..3888
                     /locus_tag="intron"
                     /label="intron"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1630..1899
                     /locus_tag="GFP 149-238(end)"
                     /label="GFP 149-238(end)"
                     /ApEinfo_label="GFP 149-238(end)"
                     /ApEinfo_fwdcolor="#64ff00"
                     /ApEinfo_revcolor="#64ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          1324..1365
                     /locus_tag="intron(1)"
                     /label="intron(1)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     UTR             4016..4750
                     /locus_tag="unc-54 3UTR"
                     /label="unc-54 3UTR"
                     /ApEinfo_label="unc-54 3UTR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_signal     1475..1489
                     /locus_tag="EK recognition"
                     /label="EK recognition"
                     /ApEinfo_label="EK recognition"
                     /ApEinfo_fwdcolor="#0004ff"
                     /ApEinfo_revcolor="#0004ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     exon            4499..4653
                     /locus_tag="aex-5 exon1"
                     /label="aex-5 exon1"
                     /ApEinfo_label="aex-5 exon1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        27..1305
                     /locus_tag="punc-25"
                     /label="punc-25"
                     /ApEinfo_label="punc-25"
                     /ApEinfo_fwdcolor="#41ffb2"
                     /ApEinfo_revcolor="#41ffb2"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     variation       1593..1594
                     /locus_tag="del tat->tt"
                     /label="del tat->tt"
                     /ApEinfo_label="del tat->tt"
                     /ApEinfo_fwdcolor="#ffeb14"
                     /ApEinfo_revcolor="#ffeb14"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            1304..1305
                     /gene="unc-25"
                     /locus_tag="unc-25"
                     /label="unc-25"
                     /ApEinfo_label="unc-25"
                     /ApEinfo_fwdcolor="#817c7c"
                     /ApEinfo_revcolor="#817c7c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(2935..3090,3142..3335,3387..3450,3452..3579,
                     3631..3798)
                     /locus_tag="worm mCherry"
                     /label="worm mCherry"
                     /ApEinfo_label="worm mCherry"
                     /ApEinfo_fwdcolor="#ff0062"
                     /ApEinfo_revcolor="#ff0062"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      6175..6857
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     7225..7245
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            1304..1305
                     /gene="unc-25"
                     /locus_tag="unc-25(1)"
                     /label="unc-25(1)"
                     /ApEinfo_label="unc-25"
                     /ApEinfo_fwdcolor="#817c7c"
                     /ApEinfo_revcolor="#817c7c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2410..2844
                     /locus_tag="CaM domain(1)"
                     /label="CaM domain(1)"
                     /ApEinfo_label="CaM domain"
                     /ApEinfo_fwdcolor="#ff9d00"
                     /ApEinfo_revcolor="#ff9d00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             5418..6077
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1304..1305
                     /gene="unc-25"
                     /standard_name="Y37D8A.23b"
                     /note="C. elegans UNC-25 protein; contains similarity to
                     Pfam domain PF00282 Pyridoxal-dependent decarboxylase
                     conserved domain contains similarity to Interpro domains
                     IPR002129 (Pyridoxal phosphate-dependent decarboxylase),
                     IPR015421 (Pyridoxal phosphate-dependent transferase,
                     major region, subdomain 1)"
                     /codon_start=1
                     /product="C. elegans protein Y37D8A.23b, confirmed by
                     transcript evidence"
                     /protein_id="CAD45605.1"
                     /db_xref="GI:22859112"
                     /db_xref="GOA:Q8I4E0"
                     /db_xref="InterPro:IPR002129"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015424"
                     /db_xref="UniProtKB/TrEMBL:Q8I4E0"
                     /db_xref="WormBase:WBGene00006762"
                     /translation="MSSAAADESDAVLENLIAKEILPQTGNWEGTEEFLNRIVQVLLK
                     YIKDQNDRDQKILEFHHPDKMQMLMDLSIPEKPESLLKLVKSCEDVLRLGVRTGHPRF
                     FNQISCGLDLVSMAGEWLTATANTNMFTYEIAPVFILMEKSVMARMWEAVGWDPEKAD
                     GIFAPGGAIANLYAMNAARHQLWPRSKHLGMKDIPTLCCFTSEDSHYSIKSASAVLGI
                     GADYCFNIPTDKNGKMIPEALEAKIIECKKEGLTPFFACCTAGSTVYGAFDPLERVAN
                     ICERHKLWFHVDAAWGGGMLLSPEHRYKLAGIERANSVTWNPHKLMGALLQCSACLFR
                     QDGLLFQCNQMSADYLFQQDKPYDVSFDTGDKAIQCGRHNDVFKLWLMWKSKGMEGYR
                     QQINKLMDLANYFTRRIKETEGFELIIENVSFARIPEHLFLVRAFKDSKLGAC"
                     /locus_tag="C. elegans protein Y37D8A.23b, confirmed by
                     transc"
                     /label="C. elegans protein Y37D8A.23b, confirmed by
                     transc"
                     /ApEinfo_label="C. elegans protein Y37D8A.23b, confirmed
                     by transc"
                     /ApEinfo_fwdcolor="#ff9d00"
                     /ApEinfo_revcolor="#ff9d00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     variation       3451..3451
                     /locus_tag="P139S(c to t sequenced data pJH1817OZM2281)"
                     /label="P139S(c to t sequenced data pJH1817OZM2281)"
                     /ApEinfo_label="P139S(c to t sequenced data
                     pJH1817OZM2281)"
                     /ApEinfo_fwdcolor="#ffeb14"
                     /ApEinfo_revcolor="#ffeb14"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          3965..4015
                     /locus_tag="intron(2)"
                     /label="intron(2)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1304..1305
                     /gene="unc-25"
                     /standard_name="Y37D8A.23a"
                     /note="C. elegans UNC-25 protein; contains similarity to
                     Pfam domain PF00282 Pyridoxal-dependent decarboxylase
                     conserved domain contains similarity to Interpro domains
                     IPR002129 (Pyridoxal phosphate-dependent decarboxylase),
                     IPR015421 (Pyridoxal phosphate-dependent transferase,
                     major region, subdomain 1)"
                     /codon_start=1
                     /product="C. elegans protein Y37D8A.23a, confirmed by
                     transcript evidence"
                     /protein_id="CAA21537.1"
                     /db_xref="GI:3925209"
                     /db_xref="GOA:Q9XTP4"
                     /db_xref="InterPro:IPR002129"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015424"
                     /db_xref="UniProtKB/TrEMBL:Q9XTP4"
                     /db_xref="WormBase:WBGene00006762"
                     /translation="MSSAAADESDAVLENLIAKEILPQTGNWEGTEEFLNRIVQVLLK
                     YIKDQNDRDQKILEFHHPDKMQMLMDLSIPEKPESLLKLVKSCEDVLRLGVRTGHPRF
                     FNQISCGLDLVSMAGEWLTATANTNMFTYEIAPVFILMEKSVMARMWEAVGWDPEKAD
                     GIFAPGGAIANLYAMNAARHQLWPRSKHLGMKDIPTLCCFTSEDSHYSIKSASAVLGI
                     GADYCFNIPTDKNGKMIPEALEAKIIECKKEGLTPFFACCTAGSTVYGAFDPLERVAN
                     ICERHKLWFHVDAAWGGGMLLSPEHRYKLAGIERANSVTWNPHKLMGALLQCSACLFR
                     QDGLLFQCNQMSADYLFQQDKPYDVSFDTGDKAIQCGRHNDVFKLWLMWKSKGMEGYR
                     QQINKLMDLANYFTRRIKETEGFELIIENPEFLNICFWYVPSKIRNLEPAEMRARLEK
                     IAPKIKAGMMQRGTTMVGYQPDKQRPNFFRMIISNQAITREDLDFLIKEIVDIGESLE
                     "
                     /locus_tag="C. elegans protein Y37D8A.23a, confirmed by
                     transc"
                     /label="C. elegans protein Y37D8A.23a, confirmed by
                     transc"
                     /ApEinfo_label="C. elegans protein Y37D8A.23a, confirmed
                     by transc"
                     /ApEinfo_fwdcolor="#ff9d00"
                     /ApEinfo_revcolor="#ff9d00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    7197..7219
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="cornflower blue"
                     /ApEinfo_revcolor="cornflower blue"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1409..1428
                     /locus_tag="His6"
                     /label="His6"
                     /ApEinfo_label="His6"
                     /ApEinfo_fwdcolor="#c90700"
                     /ApEinfo_revcolor="#c90700"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1212..1233
                     /locus_tag="fwd Punc25 primer"
                     /label="fwd Punc25 primer"
                     /ApEinfo_label="fwd Punc25 primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1681..1701)
                     /locus_tag="rev primer from gcamp3"
                     /label="rev primer from gcamp3"
                     /ApEinfo_label="rev primer from gcamp3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ff92f5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 AAGCTTGGGC TGCAgcatgc ggccgcgaaa tatcgatttt ttccgagata tctaaaattg
       61 aaagaaattt tcgattttct gagaaaaata cgaaaaaatc gaaaaaaaaa cccaaatttc
      121 ggttttttcg taaaaaaagt cgtaaaaatg taattttttt cctgaaaaat cggaattttt
      181 tacaaaatat cggaaaatac tcaaaaaaaa gctgaaaatt tcgattttcc ggagaaaaaa
      241 tctttttaaa aaaatatttt ttttttcaga aaataagaaa agccgaaatt tataactatt
      301 tctccggaaa ttcgaaattt ttaacgaaat atcggaataa tttttagatt tttcaagttt
      361 gactttgcga gaaaaaaatc ggaaaaacct cgattcgacg ccgaaaaatg ctccttttcg
      421 aaaagatttt tgaaatttca gaaaatcgac atgcaagcgc gctccacggc gaaatgacaa
      481 cgatgatcca ccgccctcaa aaagttgggt ctcgttaggt atttggcggt aaaactggta
      541 aaactccagt tttgcctcca acgagaccca attttttggg gcggtggtgg agcgcgcttg
      601 cacaagctga aagcattttt ctgcgactcg ataatatttt gaaaacctgt gtcaattctc
      661 gaaattcttt ttttaaaaaa taatcccgag cttctctcag tcctcctcta tgaggatgtt
      721 cctttttttt ggtttttcaa ttttttttaa aattccaaat ttctgttgtg caattcactt
      781 ccccccaaga aatcccaaaa atccccagtt ttccccaaaa atgttccgtt ttcatgtgat
      841 ttttccccca tttttaaaac atttttttga ctttttttta aaatgattat tattattgtt
      901 ttctatttca tggccggtaa attatttttt ttctttcttt ttttttgctc ttttttttca
      961 agaattttcg aattgtttga agggctgctc atctaatctt ttgtcatttt gttctgatgc
     1021 catcatttct gagaggacct ttgaagactc gtcacgaaac gggagggggg ctcaagtgag
     1081 cattattatt attattattg tcgcaaaaag tttaccccgg gctccccctg gctcccctct
     1141 ttgagcaagg gtttaagggc tcattttgat gacgaattgc tcattgggat tatagtcacg
     1201 cccctctttt ggagcaacta cacaactgag ccacagtaat ccttgggggc ggggtcagta
     1261 ggaccccctc cggaataggg aaaagctcag ttcaccgcca aaaatTGGCC AAAGGACCCA
     1321 AAGgtatgtt tcgaatgata ctaacataac atagaacatt ttcagGAGGA CCCTTGGCTA
     1381 GCGTCGACGg atccaaaaaA TGGGTTCTCA TCATCATCAT CATCATGGTA TGGCTAGCAT
     1441 GACTGGTGGA CAGCAAATGG GTCGGGATCT GTACGACGAT GACGATAAGG ATCTCGCCAC
     1501 CATGGTCGAC TCATCACGTC GTAAGTGGAA TAAGACAGGT CACGCAGTCA GAGCTATAGG
     1561 TCGGCTGAGC TCACTCGAGg taagtttaaa cattatatac taactaacgg attatattta
     1621 aattttcagA ACGTCTATAT CAAGGCCGAC AAGCAGAAGA ACGGCATCAA GGCGAACTTC
     1681 AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCTACCACTA CCAGCAGAAC
     1741 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CGTGCAGTCC
     1801 AAACTTTCGA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
     1861 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG GCGGTACCGG AGgtaagttt
     1921 aaacagttcg gtactaacta accctgatta tttaaatttt cagGGAGCAT GGTGAGCAAG
     1981 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
     2041 GGCCACAAGT TCAGCGTGTC CGGCGAGGGT GAGGGCGATG CCACCTACGG CAAGCTGACC
     2101 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
     2161 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
     2221 TTCAAGTCCG CCATGCCCGA AGGCTACATC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
     2281 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
     2341 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
     2401 AACACGCGTG ACCAACTGAC TGAAGgtaag tttaaacttg gacttactaa ctaaccatac
     2461 atatttaaat tttcagAGCA GATCGCAGAA TTTAAAGAGG CTTTCTCCCT ATTTGACAAG
     2521 GACGGGGATG GGACAATAAC AACCAAGGAG CTGGGGACGG TGATGCGGTC TCTGGGGCAG
     2581 AACCCCACAG AAGCAGAGCT GCAGGACATG ATCAATGAAG TAGATGCCGA CGGTGACGGC
     2641 ACAATCGACT TCCCTGAGTT CCTGACAATG ATGGCAAGAA AAATGAAAGA CACAGACAGT
     2701 GAAGAAGAAA TTAGAGAAGC GTTCCGTGTG TTTGATAAGG ATGGCAATGG CTACATCAGT
     2761 GCAGCAGAGC TTCGCCACGT GATGACAAAC CTTGGAGAGA AGTTAACAGA TGAAGAGGTT
     2821 GATGAAATGA TCAGGGAAGC AGACATCGAT GGGGATGGTC AGGTAAACTA CGAAGAGTTT
     2881 GTACAAATGA TGACAGCGAA Gggaggacgt ggaggatctg gcggcgcgcc aaaaATGGTC
     2941 TCAAAGGGTG AAGAAGATAA CATGGCAATT ATTAAAGAGT TTATGCGTTT CAAGGTGCAT
     3001 ATGGAGGGAT CTGTCAATGG GCATGAGTTT GAAATTGAAG GTGAAGGAGA AGGCCGACCA
     3061 TATGAGGGAA CACAAACCGC AAAACTAAAG gtaagtttaa acatatatat actaactaac
     3121 cctgattatt taaattttca gGTAACTAAA GGCGGACCAT TACCATTCGC CTGGGACATC
     3181 CTCTCTCCAC AGTTCATGTA TGGAAGTAAA GCTTATGTTA AACATCCGGC AGATATACCA
     3241 GATTATTTGA AACTTTCATT CCCGGAGGGT TTTAAGTGGG AACGCGTAAT GAATTTTGAA
     3301 GACGGAGGAG TTGTTACAGT GACGCAAGAC TCAAGGTAAG TTTAAACAGT TCGGTACTAA
     3361 CTAACCATAC ATATTTAAAT TTTCAGCCTC CAAGATGGAG AATTTATTTA TAAAGTCAAA
     3421 CTTCGAGGAA CGAATTTCCC CTCGGATGGA tCTGTTATGC AGAAGAAGAC TATGGGATGG
     3481 GAAGCTTCAA GTGAAAGAAT GTACCCTGAA GACGGTGCTC TTAAGGGAGA GATTAAACAA
     3541 CGTCTTAAAT TGAAAGATGG AGGACATTAC GATGCTGAGg taagtttaaa catgatttta
     3601 ctaactaact aatctgattt aaattttcag GTGAAGACAA CTTACAAAGC CAAAAAACCA
     3661 GTTCAGCTGC CAGGAGCGTA CAATGTTAAT ATTAAACTGG ATATCACCTC CCACAACGAG
     3721 GATTACACTA TCGTTGAGCA ATATGAAAGA GCTGAAGGGC GGCACTCGAC AGGTGGCATG
     3781 GATGAATTGT ATAAGTAGGA GCTCCCGAGA TCTTCAGCGC TCAGCATTCG TAGAATTgta
     3841 agtttaaact ttctcatact aactaacgcg ctctatttaa attttcagAA TTCCAACTGA
     3901 GCGCCGGTCG CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT
     3961 CAGAgtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagCATCT
     4021 CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC
     4081 TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA AACTTCTTCT TAATTTCTTT
     4141 GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA
     4201 TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT
     4261 ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTTTAC TTCTGATAAA
     4321 TTTTTTTTGA AACATCATAG AAAAAACCGC ACACAAAATA CCTTATCATA TGTTACGTTT
     4381 CAGTTTATGA CCGCAATTTT TATTTCTTCG CACGTCTGGG CCTCTCATGA CGTCAAATCA
     4441 TGCTCATCGT GAAAAAGTTT TGGAGTATTT TTGGAATTTT TCAATCAAGT GAAAGTTTAT
     4501 GAAATTAATT TTCCTGCTTT TGCTTTTTGG GGGTTTCCCC TATTGTTTGT CAAGAGTTTC
     4561 GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC
     4621 GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT
     4681 GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC
     4741 ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA
     4801 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA
     4861 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG
     4921 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT
     4981 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC
     5041 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT
     5101 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
     5161 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
     5221 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG
     5281 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC
     5341 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG
     5401 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC
     5461 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
     5521 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT
     5581 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG
     5641 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG
     5701 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC
     5761 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
     5821 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT
     5881 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC
     5941 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA
     6001 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT
     6061 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
     6121 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
     6181 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     6241 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     6301 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     6361 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
     6421 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     6481 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     6541 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     6601 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC
     6661 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     6721 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     6781 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
     6841 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
     6901 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
     6961 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
     7021 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
     7081 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
     7141 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
     7201 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt
     7261 aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA GCTTAAAAAT
     7321 GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT 
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