LOCUS       OA-1050T-Ubiq-Ca       11134 bp DNA     linear   SYN 02-SEP-2022
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(854..1714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     old_sequence    2319..3026
                     /label=flanking sequence
     misc_binding    complement(3027..4024)
                     /label=piggyBacR
     misc_binding    4073..4357
                     /label=attB site
                     /note="attB site"
     misc_feature    4366..4786
                     /label=AAEL006511 Ubiquitin-L-40 Promoter
                     /note="AAEL006511 Ubiquitin-L-40 Promoter region from 
                     Anderson et. Al 2010"
     misc_feature    4794..4796
                     /label=ATG
                     /note="ATG"
     misc_feature    4797..4829
                     /label=NLS
                     /note="NLS"
     CDS             4800..4820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4830..7727
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     misc_feature    7734..7766
                     /label=NLS
                     /note="NLS"
     CDS             7737..7757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7767..7793
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    7802..8478
                     /label=p10-3'-UTR-Rubin
                     /note="p10-3'-UTR-Rubin"
     misc_feature    8487..9027
                     /label=Opie2 promoter
                     /note="Opie2 promoter"
     CDS             9028..9708
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     misc_feature    9355..9363
                     /label=pt mutation
                     /note="pt mutation"
     misc_feature    9709..9949
                     /label=Sv40
                     /note="Sv40"
     polyA_signal    9822..9943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_binding    9961..10661
                     /label=piggyBacL
     old_sequence    10662..10790
                     /label=flanking seq
     misc_feature    complement(10791..11134)
                     /label=misc_feature 1
     primer_bind     complement(10827..10843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10851..10867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10875..10905)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10920..10941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggtcct aggtcgacga
     4081 tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg
     4141 gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg tggcggtagt
     4201 tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg
     4261 tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg
     4321 tcagcgggtt ctcgacggtc acggcgggca tgtcgacgcg gccgcagcgt gaaccttgca
     4381 tgcgtgcaca gaatttgatt gcatttcatg accgggtatc cattcggcgc cggccactga
     4441 actgaaattc agttttgaca gctccactga cttgaagctc tttttcaccg aaacacatct
     4501 cggaagagac acgttgggaa agagtaagtt taatttttgt cgtttttcgt ggatttttag
     4561 tgataattcg tggtttttcg ttgttagcaa tctaaatcat tgatagatca agttagaaat
     4621 tgtgaaatat tgctaaaata cctacattat tcgtagttta ttcgatttgt tgtagttgat
     4681 gcattctaac ctcaatgtgg tttcgtctcc gtccaattcc gatgttattg tccagttcgt
     4741 taatttttgg tgttttctcc ccgacttttc cacagactga tccaagttaa ttaatgagcc
     4801 ccaagaagaa gagaaaggtg gaggccagca tcgaaaaaaa aaagtccttc gccaagggca
     4861 tgggcgtgaa gtccacactc gtgtccggct ccaaagtgta catgacaacc ttcgccgaag
     4921 gcagcgacgc caggctggaa aagatcgtgg agggcgacag catcaggagc gtgaatgagg
     4981 gcgaggcctt cagcgctgaa atggccgata aaaacgccgg ctataagatc ggcaacgcca
     5041 aattcagcca tcctaagggc tacgccgtgg tggctaacaa ccctctgtat acaggacccg
     5101 tccagcagga tatgctcggc ctgaaggaaa ctctggaaaa gaggtacttc ggcgagagcg
     5161 ctgatggcaa tgacaatatt tgtatccagg tgatccataa catcctggac attgaaaaaa
     5221 tcctcgccga atacattacc aacgccgcct acgccgtcaa caatatctcc ggcctggata
     5281 aggacattat tggattcggc aagttctcca cagtgtatac ctacgacgaa ttcaaagacc
     5341 ccgagcacca tagggccgct ttcaacaata acgataagct catcaacgcc atcaaggccc
     5401 agtatgacga gttcgacaac ttcctcgata accccagact cggctatttc ggccaggcct
     5461 ttttcagcaa ggagggcaga aattacatca tcaattacgg caacgaatgc tatgacattc
     5521 tggccctcct gagcggactg aggcactggg tggtccataa caacgaagaa gagtccagga
     5581 tctccaggac ctggctctac aacctcgata agaacctcga caacgaatac atctccaccc
     5641 tcaactacct ctacgacagg atcaccaatg agctgaccaa ctccttctcc aagaactccg
     5701 ccgccaacgt gaactatatt gccgaaactc tgggaatcaa ccctgccgaa ttcgccgaac
     5761 aatatttcag attcagcatt atgaaagagc agaaaaacct cggattcaat atcaccaagc
     5821 tcagggaagt gatgctggac aggaaggata tgtccgagat caggaaaaat cataaggtgt
     5881 tcgactccat caggaccaag gtctacacca tgatggactt tgtgatttat aggtattaca
     5941 tcgaagagga tgccaaggtg gctgccgcca ataagtccct ccccgataat gagaagtccc
     6001 tgagcgagaa ggatatcttt gtgattaacc tgaggggctc cttcaacgac gaccagaagg
     6061 atgccctcta ctacgatgaa gctaatagaa tttggagaaa gctcgaaaat atcatgcaca
     6121 acatcaagga atttagggga aacaagacaa gagagtataa gaagaaggac gcccctagac
     6181 tgcccagaat cctgcccgct ggccgtgatg tttccgcctt cagcaaactc atgtatgccc
     6241 tgaccatgtt cctggatggc aaggagatca acgacctcct gaccaccctg attaataaat
     6301 tcgataacat ccagagcttc ctgaaggtga tgcctctcat cggagtcaac gctaagttcg
     6361 tggaggaata cgcctttttc aaagactccg ccaagatcgc cgatgagctg aggctgatca
     6421 agtccttcgc tagaatggga gaacctattg ccgatgccag gagggccatg tatatcgacg
     6481 ccatccgtat tttaggaacc aacctgtcct atgatgagct caaggccctc gccgacacct
     6541 tttccctgga cgagaacgga aacaagctca agaaaggcaa gcacggcatg agaaatttca
     6601 ttattaataa cgtgatcagc aataaaaggt tccactacct gatcagatac ggtgatcctg
     6661 cccacctcca tgagatcgcc aaaaacgagg ccgtggtgaa gttcgtgctc ggcaggatcg
     6721 ctgacatcca gaaaaaacag ggccagaacg gcaagaacca gatcgacagg tactacgaaa
     6781 cttgtatcgg aaaggataag ggcaagagcg tgagcgaaaa ggtggacgct ctcacaaaga
     6841 tcatcaccgg aatgaactac gaccaattcg acaagaaaag gagcgtcatt gaggacaccg
     6901 gcagggaaaa cgccgagagg gagaagttta aaaagatcat cagcctgtac ctcaccgtga
     6961 tctaccacat cctcaagaat attgtcaata tcaacgccag gtacgtcatc ggattccatt
     7021 gcgtcgagcg tgatgctcaa ctgtacaagg agaaaggcta cgacatcaat ctcaagaaac
     7081 tggaagagaa gggattcagc tccgtcacca agctctgcgc tggcattgat gaaactgccc
     7141 ccgataagag aaaggacgtg gaaaaggaga tggctgaaag agccaaggag agcattgaca
     7201 gcctcgagag cgccaacccc aagctgtatg ccaattacat caaatacagc gacgagaaga
     7261 aagccgagga gttcaccagg cagattaaca gggagaaggc caaaaccgcc ctgaacgcct
     7321 acctgaggaa caccaagtgg aatgtgatca tcagggagga cctcctgaga attgacaaca
     7381 agacatgtac cctgttcaga aacaaggccg tccacctgga agtggccagg tatgtccacg
     7441 cctatatcaa cgacattgcc gaggtcaatt cctacttcca actgtaccat tacatcatgc
     7501 agagaattat catgaatgag aggtacgaga aaagcagcgg aaaggtgtcc gagtacttcg
     7561 acgctgtgaa tgacgagaag aagtacaacg ataggctcct gaaactgctg tgtgtgcctt
     7621 tcggctactg tatccccagg tttaagaacc tgagcatcga ggccctgttc gataggaacg
     7681 aggccgccaa gttcgacaag gagaaaaaga aggtgtccgg caattccgga tccggaccta
     7741 agaaaaagag gaaggtggcg gccgcttacc catacgatgt tccagattac gcttaagcta
     7801 gctagaatga atcgttttta aaataacaaa tcaattgttt tataatattc gtacgattct
     7861 ttgattatgt aataaaatgt gatcattagg aagattacga aaaatataaa aaatatgagt
     7921 tctgtgtgta taacaaatgc tgtaaacgcc acaattgtgt ttgttgcaaa taaacccatg
     7981 attatttgat taaaattgtt gttttctttg ttcatagaca atagtgtgtt ttgcctaaac
     8041 gtgtactgca taaactccat gcgagtgtat agcgagctag tggctaacgc ttgccccacc
     8101 aaagtagatt cgtcaaaatc ctcaatttca tcaccctcct ccaagtttaa catttggccg
     8161 tcggaattaa cttctaaaga tgccacataa tctaataaat gaaatagaga ttcaaacgtg
     8221 gcgtcatcgt ccgtttcgac catttccgaa aagaactcgg gcataaactc tatgatttct
     8281 ctggacgtgg tgttgtcgaa actctcaaag tacgcagtca ggaacgtgcg cgacatgtcg
     8341 tcgggaaact cgcgcggaaa catgttgttg taaccgaacg ggtcccatag cgccaaaacc
     8401 aaatctgcca gcgtcaatag aatgagcacg atgccgacaa tggagctggc ttggatagcg
     8461 attcgagtta acggccgggg cgcgcccata ctcggtggcc tccccaccac caactttttt
     8521 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc
     8581 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac
     8641 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc
     8701 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt
     8761 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca
     8821 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc
     8881 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag
     8941 catctgttac agcgacacaa catgagccgg tccaacaacg ccaacgcgcc cacgccatcc
     9001 aaccgccgcc gcaacctgtc tctggtgatg gtgcgctcct ccaagaacgt catcaaggag
     9061 ttcatgcgct tcaaggtgcg catggagggc accgtgaacg gccacgagtt cgagatcgag
     9121 ggcgagggcg agggccgccc ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag
     9181 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag
     9241 gtgtacgtga agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc
     9301 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     9361 tcctccctgc aggacggctg cttcatctac aaggtgaagt tcatcggcgt gaacttcccc
     9421 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
     9481 tacccccgcg acggcgtgct gaagggcgag atccacaagg ccctgaagct gaaggacggc
     9541 ggccactacc tggtggagtt caagtccatc tacatggcca agaagcccgt gcagctgccc
     9601 ggctactact acgtggactc caagctggac atcacctccc acaacgagga ctacaccatc
     9661 gtggagcagt acgagcgcac cgagggccgc caccacctgt tcctgtagga ctctagatca
     9721 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     9781 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     9841 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9901 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaagcttc taggccggcc
     9961 gatctcggat ctgacaatgt tcagtgcaga gactcggcta cgcctcgtgg actttgaagt
    10021 tgaccaacaa tgtttattct tacctctaat agtcctctgt ggcaaggtca agattctgtt
    10081 agaagccaat gaagaacctg gttgttcaat aacattttgt tcgtctaata tttcactacc
    10141 gcttgacgtt ggctgcactt catgtacctc atctataaac gcttcttctg tatcgctctg
    10201 gacgtcatct tcacttacgt gatctgatat ttcactgtca gaatcctcac caacaagctc
    10261 gtcatcgctt tgcagaagag cagagaggat atgctcatcg tctaaagaac tacccatttt
    10321 attatatatt agtcacgata tctataacaa gaaaatatat atataataag ttatcacgta
    10381 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
    10441 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
    10501 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
    10561 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
    10621 ttttacgcag actatctttc tagggttaaa aaagatttgc gctttactcg acctaaactt
    10681 taaacacgtc atagaatctt cgtttgacaa aaaccacatt gtggccaagc tgtgtgacgc
    10741 gacgcgcgct aaagaatggc aaaccaagtc gcgcgagcgt cgactctaga ggatccccgg
    10801 gtaccgagct cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10861 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10921 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    10981 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    11041 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    11101 gcggtatcag ctcactcaaa ggcggtaata cggt
//
