LOCUS       pVM62                   8608 bp ds-DNA     circular     05-SEP-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             239..952
                     /label="mRuby2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     Terminator      996..1098
                     /label="Terminator 0"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     AbR             1553..2287
                     /label="eryR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2768..3067
                     /label="repD (pAMB)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3110..4600
                     /label="repE (pAMB)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             5343..5876
                     /label="acc3 (GmR)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5962..6197
                     /label="OriT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            complement(6206..6868)
                     /label="BBR1 Rep"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    7863..7982
                     /label="Terminator 1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(8019..37)
                     /label="tetR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 TGTTAATCAC TTTACTTTTA TCTAATCTAG ACATCATTAA TTCCTCCTTT TTGTTGACAT
       61 TATATCATTG ATAGAGTTAT TTGTCAAACT AGATTTTTAT TTCGATGCCC TGGACTTCAT
      121 GAAAAACTAA AAAAAATATT GACACTCTAT CATTGATAGA GTATAATTAA AATAAGCTTG
      181 ATCGTAGCGT TAACAGATCT GAGCTCCTGC AGTAAGCTGA GGCGATAGGA GGAATACAAT
      241 GGTGTCTAAG GGCGAAGAGC TGATCAAGGA AAATATGCGT ATGAAGGTGG TCATGGAAGG
      301 TTCGGTCAAC GGCCACCAAT TCAAATGCAC AGGTGAAGGA GAAGGCAATC CGTACATGGG
      361 AACTCAAACC ATGAGGATCA AAGTCATCGA GGGAGGACCC CTGCCATTTG CCTTTGACAT
      421 TCTTGCCACG TCGTTCATGT ATGGCAGCCG TACTTTTATC AAGTACCCGA AAGGCATTCC
      481 TGATTTCTTT AAACAGTCCT TTCCTGAGGG TTTTACTTGG GAAAGAGTTA CGAGATACGA
      541 AGATGGTGGA GTCGTCACCG TCATGCAGGA CACCAGCCTT GAGGATGGCT GTCTCGTTTA
      601 CCACGTCCAA GTCAGAGGGG TAAACTTTCC CTCCAATGGT CCCGTGATGC AGAAGAAGAC
      661 CAAGGGTTGG GAGCCTAATA CAGAGATGAT GTATCCAGCA GATGGTGGTC TGAGGGGATA
      721 CACTCATATG GCACTGAAAG TTGATGGTGG TGGCCATCTG TCTTGCTCTT TCGTAACAAC
      781 TTACAGGTCA AAAAAGACCG TCGGGAACAT CAAGATGCCC GGTATCCATG CCGTTGATCA
      841 CCGCCTGGAA AGGTTAGAGG AAAGTGACAA TGAAATGTTC GTAGTACAAC GCGAACACGC
      901 AGTTGCCAAG TTCGCCGGGC TTGGTGGTGG GATGGACGAG CTGTACAAGT AAATCGCACC
      961 TCAGCGTCGT GACTGGGAAA ACCCTGGCGA CTAGTCTTGG ACTCCTGTTG ATAGATCCAG
     1021 TAATGACCTC AGAACTCCAT CTGGATTTGT TCAGAACGCT CGGTTGCCGC CGGGCGTTTT
     1081 TTATTGGTGA GAATCCAGCC TGCAGGATCG CACCTgcaac cctcaaaatt gaatgagaca
     1141 tgctacacct ccggataata aatatatata aacgtatata gatttcataa agtctaacac
     1201 actagactta tttacttcgt aattaagtcg ttaaaccgtg tgctctacga ccaaaactat
     1261 aaaaccttta agaactttct ttttttacaa gaaaaaagaa attagataaa tctctcatat
     1321 cttttattca ataatcgcat ccgattgcag tataaattta acgatcactc atcatgttca
     1381 tatttatcag aggtcgtgct ataattatac taattttata aggaggaaaa aatatgggca
     1441 tttttagtat ttttgtaatc agcacagttc attatcaacc aaacaaaaaa taagtggtta
     1501 taatgaatcg ttaataagca aaattcatat aaccaaatta aagagggtta taatgaacga
     1561 gaaaaatata aaacacagtc aaaactttat tacttcaaaa cataatatag ataaaataat
     1621 gacaaatata agattaaatg aacatgataa tatctttgaa atcggctcag gaaaaggcca
     1681 ttttaccctt gaattagtaa agaggtgtaa tttcgtaact gccattgaaa tagaccataa
     1741 attatgcaaa actacagaaa ataaacttgt tgatcacgat aatttccaag ttttaaacaa
     1801 ggatatattg cagtttaaat ttcctaaaaa ccaatcctat aaaatatatg gtaatatacc
     1861 ttataacata agtacggata taatacgcaa aattgttttt gatagtatag ctaatgagat
     1921 ttatttaatc gtggaatacg ggtttgctaa aagattatta aatacaaaac gctcattggc
     1981 attactttta atggcagaag ttgatatttc tatattaagt atggttccaa gagaatattt
     2041 tcatcctaaa cctaaagtga atagctcact tatcagatta agtagaaaaa aatcaagaat
     2101 atcacacaaa gataaacaaa agtataatta tttcgttatg aaatgggtta acaaagaata
     2161 caagaaaata tttacaaaaa atcaatttaa caattcctta aaacatgcag gaattgacga
     2221 tttaaacaat attagctttg aacaattctt atctcttttc aatagctata aattatttaa
     2281 taagtaagtt aagggatgca taaactgcat cccttaacag tgcTGGTACC ccagaaacag
     2341 aatcagaaca agtaacattg gatttagaaa tggaagaaga aaaaagcaat gacttcgtgt
     2401 gaataatgca cgaaatcgtt gcttattttt tttaaaagcg gtatactaga tataacgaaa
     2461 caacgaactg aatagaaacg aaaaaagagc catgacacat ttataaaatg tttgacgaca
     2521 ttttataaat gcatagcccg ataagattgc caaaccaacg cttatcagtt agtcagatga
     2581 actcttccct cgtaagaagt tatttaatta actttgtttg aagacggtat ataaccgtac
     2641 tatcattata tagggaaatc agagagtttt caagtatcta agctactgaa tttaagaatt
     2701 gttaagcaat caatcggaaa tcgtttgatt gctttttttg tattcattta tagaaggtgg
     2761 agtttgtatg aatcatgatg aatgtaaaac ttatataaaa aatagtttat tggagataag
     2821 aaaattagca aatatctata cactagaaac gtttaagaaa gagttagaaa agagaaatat
     2881 ctacttagaa acaaaatcag ataagtattt ttcttcggag ggggaagatt atatatataa
     2941 gttaatagaa aataacaaaa taatttattc gattagtgga aaaaaattga cttataaagg
     3001 aaaaaaatct ttttcaaaac atgcaatatt gaaacagttg aatgaaaaag caaaccaagt
     3061 taattaaaca acctatttta taggatttat aggaaaggag aacagctgaa tgaatatccc
     3121 ttttgttgta gaaactgtgc ttcatgacgg cttgttaaag tacaaattta aaaatagtaa
     3181 aattcgctca atcactacca agccaggtaa aagcaaaggg gctatttttg cgtatcgctc
     3241 aaaatcaagc atgattggcg gtcgtggtgt tgttctgact tccgaggaag cgattcaaga
     3301 aaatcaagat acatttacac attggacacc caacgtttat cgttatggaa cgtatgcaga
     3361 cgaaaaccgt tcatacacga aaggacattc tgaaaacaat ttaagacaaa tcaatacctt
     3421 ctttattgat tttgatattc acacggcaaa agaaactatt tcagcaagcg atattttaac
     3481 aaccgctatt gatttaggtt ttatgcctac tatgattatc aaatctgata aaggttatca
     3541 agcatatttt gttttagaaa cgccagtcta tgtgacttca aaatcagaat ttaaatctgt
     3601 caaagcagcc aaaataattt cgcaaaatat ccgagaatat tttggaaagt ctttgccagt
     3661 tgatctaacg tgtaatcatt ttggtattgc tcgcatacca agaacggaca atgtagaatt
     3721 ttttgatcct aattaccgtt attctttcaa agaatggcaa gattggtctt tcaaacaaac
     3781 agataataag ggctttactc gttcaagtct aacggtttta agcggtacag aaggcaaaaa
     3841 acaagtagat gaaccctggt ttaatctctt attgcacgaa acgaaatttt caggagaaaa
     3901 gggtttaata gggcgtaata acgtcatgtt taccctctct ttagcctact ttagttcagg
     3961 ctattcaatc gaaacgtgcg aatataatat gtttgagttt aataatcgat tagatcaacc
     4021 cttagaagaa aaagaagtaa tcaaaattgt tagaagtgcc tattcagaaa actatcaagg
     4081 ggctaatagg gaatacatta ccattctttg caaagcttgg gtatcaagtg atttaaccag
     4141 taaagattta tttgtccgtc aagggtggtt taaattcaag aaaaaaagaa gcgaacgtca
     4201 acgtgttcat ttgtcagaat ggaaagaaga tttaatggct tatattagcg aaaaaagcga
     4261 tgtatacaag ccttatttag tgacgaccaa aaaagagatt agagaagtgc taggcattcc
     4321 tgaacggaca ttagataaat tgctgaaggt actgaaggcg aatcaggaaa ttttctttaa
     4381 gattaaacca ggaagaaatg gtggcattca acttgctagt gttaaatcat tgttgctatc
     4441 gatcattaaa gtaaaaaaag aagaaaaaga aagctatata aaggcgctga caaattcttt
     4501 tgacttagag catacattca ttcaagagac tttaaacaag ctagcagaac gccctaaaac
     4561 ggacacacaa ctcgatttgt ttagctatga tacaggctga aaataaaacc cgcactatgc
     4621 cattacattt atatctatga tacgtgtttg ttttttcttt gctgtttagc gaatgattag
     4681 cagaaatata cagagtaaga ttttaattaa ttattagggg gagaaggaga gagtagcccg
     4741 aaaactttta gttggcttgg actgaacgaa gtgagggaaa ggctactaaa acgtcgaggg
     4801 gcagtgagag cgaagcgaac acttgatttt ttaattttct atcttttata ggtcattaga
     4861 gtatacttat ttgtcctata aactatttag cagcataata gatttattga ataggtcatt
     4921 taagttgagc atattagagg aggaaaatct tggagaaata tttgaagaac ccgattacat
     4981 ggattggatt agttcttgtg gttacgtggt ttttaactaa aagtagtgaa tttttgattt
     5041 ttggtgtgtg tgtcttgttg ttagtatttg ctagtcaaag tgattaaata gatctgaaTg
     5101 ggtccccaat aattacgatt taaatttgac ataagcctgt tcggttcgta aactgtaatg
     5161 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     5221 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgtacagc ctatgcctcg
     5281 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     5341 cgatgttacg cagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag
     5401 gtggctcaag tatgggcatc attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca
     5461 tgcgggctgc tcttgatctt ttcggtcgtg agttcggaga cgtagccacc tactcccaac
     5521 atcagccgga ctccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg
     5581 ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg
     5641 agcagccgcg tagtgagatc tatatctatg atctcgcagt ctccggagag caccggaggc
     5701 agggcattgc caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt
     5761 atgtgatcta cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt
     5821 tgggcatacg ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaacaat
     5881 tcgttcaagc cgagatcggc ttcccggccg cggagttgtt cggtaaattg gacaacggtc
     5941 cgcgcgttgt ccttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg
     6001 gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
     6061 ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc
     6121 gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct
     6181 ctgcgaggct ggccgtaggc cggccctacc ggcgcggcag cgttacccgt gtcggcggct
     6241 ccaacggctc gccatcgtcc agaaaacacg gctcatcggg catcggcagg cgctgctgcc
     6301 cgcgccgttc ccattcctcc gtttcggtca aggctggcag gtctggttcc atgcccggaa
     6361 tgccgggctg gctgggcggc tcctcgccgg ggccggtcgg tagttgctgc tcgcccggat
     6421 acagggtcgg gatgcggcgc aggtcgccat gccccaacag cgattcgtcc tggtcgtcgt
     6481 gatcaaccac cacggcggca ctgaacaccg acaggcgcaa ctggtcgcgg ggctggcccc
     6541 acgccacgcg gtcattgacc acgtaggccg acacggtgcc ggggccgttg agcttcacga
     6601 cggagatcca gcgctcggcc accaagtcct tgactgcgta ttggaccgtc cgcaaagaac
     6661 gtccgatgag cttggaaagt gtcttctggc tgaccaccac ggcgttctgg tggcccatct
     6721 gcgccacgag gtgatgcagc agcattgccg ccgtgggttt cctcgcaata agcccggccc
     6781 acgcctcatg cgctttgcgt tccgtttgca cccagtgacc gggcttgttc ttggcttgaa
     6841 tgccgatttc tctggactgc gtggccatgc ttatctccat gcggtagggg tgccgcacgg
     6901 ttgcggcacc atgcgcaatc agctgcaact tttcggcagc gcgacaacaa ttatgcgttg
     6961 cgtaaaagtg gcagtcaatt acagattttc tttaacctac gcaatgagct attgcggggg
     7021 gtgccgcaat gagctgttgc gtacccccct tttttaagtt gttgattttt aagtctttcg
     7081 catttcgccc tatatctagt tctttggtgc ccaaagaagg gcacccctgc ggggttcccc
     7141 cacgccttcg gcgcggctcc ccctccggca aaaagtggcc cctccggggc ttgttgatcg
     7201 actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc ccgcactcgc
     7261 cgccgtgagg ctcggggggc aggcgggcgg gcttcgccct tcgactgccc ccactcgcat
     7321 aggcttgggt cgttccaggc gcgtcaaggc caagccgctg cgcggtcgct gcgcgagcct
     7381 tgacccgcct tccacttggt gtccaaccgg caagcgaagc gcgcaggccg caggccggag
     7441 gcttttcccc agagaaaatt aaaaaaattg atggggcaag gccgcaggcc gcgcagttgg
     7501 agccggtggg tatgtggtcg aaggctgggt agccggtggg caatccctgt ggtcaagctc
     7561 gtgggcaggc gcagcctgtc catcagcttg tccagcaggg ttgtccacgg gccgagcgaa
     7621 gcgagccagc cggtggccgc tcgcggccat cgtccacata tccacgggct ggcaagggag
     7681 cgcagcgacc gcgcagggcg aagcccggag agcaagcccg tagggggAGG CGATCTCGAG
     7741 CTAGGTAACT AGAGGGACTG CGACGTTCTA AACGTTGGTC CGTCAGAAGC GCCATCCAGG
     7801 ATCACGTTAC CCCGAAAAAA AGATATCAGG AGCTCTCCTC atctgaaTGG CGCGCCTGTA
     7861 CACAGCTGTC TAGGGCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA
     7921 GATAAAACGA AAGGCCCAGT CTTTCGACTG AGCCTTTCGT TTTATTTGAT GCCTTTAATT
     7981 AAAGCGGATA ACAATTTCAC ACAGGAGGCC GATCGCGTTT AAGACCCACT TTCACATTTA
     8041 AGTTGTTTTT CTAATCCGCA TATGATCAAT TCAAGGCCGA ATAAGAAGGC TGGCTCTGCA
     8101 CCTTGGTGAT CAAATAATTC GATAGCTTGT CGTAATAATG GCGGCATACT ATCAGTAGTA
     8161 GGTGTTTCCC TTTCTTCTTT AGCGACTTGA TGCTCTTGAT CTTCCAATAC GCAACCTAAA
     8221 GTAAAATGCC CCACAGCGCT GAGTGCATAT AATGCATTCT CTAGTGAAAA ACCTTGTTGG
     8281 CATAAAAAGG CTAATTGATT TTCGAGAGTT TCATACTGTT TTTCTGTAGG CCGTGTACCT
     8341 AAATGTACTT TTGCTCCATC GCGATGACTT AGTAAAGCAC ATCTAAAACT TTTAGCGTTA
     8401 TTACGTAAAA AATCTTGCCA GCTTTCCCCT TCTAAAGGGC AAAAGTGAGT ATGGTGCCTA
     8461 TCTAACATCT CAATGGCTAA GGCGTCGAGC AAAGCCCGCT TATTTTTTAC ATGCCAATAC
     8521 AATGTAGGCT GCTCTACACC TAGCTTCTGG GCGAGTTTAC GGGTTGTTAA ACCTTCGATT
     8581 CCGACCTCAT TAAGCAGCTC TAATGCGC
//