LOCUS       Exported                6214 bp ds-DNA     circular SYN 01-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 6, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    250..268
                     /locus_tag="HSV\TK\PolyA"
                     /label=HSV\TK\PolyA
     misc_feature    599..1242
                     /locus_tag="ColE1\ORI"
                     /label=ColE1\ORI
     misc_feature    1303..1879
                     /locus_tag="CMVie enhancer promotor"
                     /label=CMVie enhancer promotor
     misc_feature    2213..2331
                     /locus_tag="Rhamnose promotor"
                     /label=Rhamnose promotor
     misc_feature    2332..2334
                     /locus_tag="start transcriptie bacteria"
                     /label=start transcriptie bacteria
                     /note="start transcriptie bacteria"
                     /note="color: #ff8000"
     misc_feature    2394..2396
                     /locus_tag="start protein"
                     /label=start protein
                     /note="start protein"
                     /note="color: #ff8000"
     misc_feature    2406..2423
                     /locus_tag="His tag"
                     /label=His tag
                     /note="His tag"
                     /note="color: #ff8000"
     misc_feature    2475..3170
                     /locus_tag="mScI"
                     /label=mScI
                     /note="mScI"
                     /note="color: #ff8000"
     misc_feature    3183..3273
                     /locus_tag="antiFRETlinker"
                     /label=antiFRETlinker
                     /note="antiFRETlinker"
                     /note="color: #ff8000"
     misc_feature    3282..3347
                     /locus_tag="P2A sequence"
                     /label=P2A sequence
                     /note="P2A sequence"
                     /note="color: #ff8000"
     misc_feature    3360..3364
                     /locus_tag="Rhamnose promotor(1)"
                     /label=Rhamnose promotor
                     /label=Rhamnose promotor(1)
     gene            3372..4634
                     /locus_tag="G-Ca-FLITS"
                     /label=G-Ca-FLITS
     misc_feature    3372..3443
                     /locus_tag="M13"
                     /label=M13
     misc_feature    3444..3452
                     /locus_tag="L1"
                     /label=L1
     gene            3453..4184
                     /locus_tag="cp150 F146Y T203Y A D mTQ2"
                     /label=cp150 F146Y T203Y A D mTQ2
     misc_feature    4185..4190
                     /locus_tag="L2"
                     /label=L2
     misc_feature    4191..4634
                     /locus_tag="CaM"
                     /label=CaM
     misc_feature    4935..4982
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
     misc_feature    5043..5071
                     /locus_tag="Bact.\promoter"
                     /label=Bact.\promoter
     misc_feature    5146..5312
                     /locus_tag="SV40\early\promoter"
                     /label=SV40\early\promoter
     misc_feature    5250..5385
                     /locus_tag="SV40\ORI"
                     /label=SV40\ORI
     misc_feature    join(5434..6214,1..14)
                     /locus_tag="KanV\Neo\resistance"
                     /label=KanV\Neo\resistance
ORIGIN
        1 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
       61 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
      121 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
      181 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
      241 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
      301 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
      361 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
      421 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
      481 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
      541 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
      601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
      661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
      781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      901 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1261 tgctcacatg ttctttcctg cgttatcccc tgaggccggc ctattaatag taatcaatta
     1321 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1381 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1441 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1501 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     1561 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     1621 cttggcagta catctacgta ttagtcatcg ctattaccat gctgatgcgg ttttggcagt
     1681 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1741 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     1801 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1861 gacgtcgttt agtgaaccgt cagatcacta gatgctttat tgcggtagtt tatcacagtt
     1921 aaattgctaa cgccagtctc gaacttaacg tgcagaagtt ggtcgtgagg cactgggcag
     1981 gtaagtatcg ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
     2041 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2101 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2161 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgaa atcaccacaa
     2221 ttcagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaaag gcccattttc
     2281 ctgtcagtaa cgagaaggtc gcgaattcag gcgcttttta gactggtcgt agggagacca
     2341 caacggtttc cctctagaaa taattttgtt taactataag aaggagatat accatgcggg
     2401 gttctcatca tcaccaccat cacagcagcg gcctggtgcc gcgcggcagc tcgctagcgc
     2461 tgccggtcgc caccatggtg agcaagggcg aggcagtgat caaggagttc atgcggttca
     2521 aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     2581 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     2641 ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc ttcatcaagc
     2701 accccgccga catccccgac tactataagc agtccttccc cgagggcttc aagtgggagc
     2761 gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc tccctggagg
     2821 acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct gacggccccg
     2881 taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac cccgaggacg
     2941 gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggcggc cgctacctgg
     3001 cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc gcctacaacg
     3061 tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg gaacagtacg
     3121 aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag tcgaccagat
     3181 ctctggccga ggcggcagct aaggaggccg cggcgaagga ggcagccgcg aaagaagcag
     3241 ccgcaaagga agcggccgcc aaggctgctg caagatctct cggatcgggt gctactaact
     3301 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggaccttcg ctagcgctgg
     3361 atcccgccac aatggtcgac tcttcacgtc gtaagtggaa taaggcaggt cacgcagtca
     3421 gagctatagg tcggctgagc tcacccgtgg ctgtctatat caccgccgac aagcagaaga
     3481 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
     3541 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3601 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     3661 tcctactgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtaccagg
     3721 gtggcagcgg tggcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3781 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3841 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     3901 tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc
     3961 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4021 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4081 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4141 acatcctggg gcacaagctg gagtacaact actatagcga cgatacgcgt gaccaactga
     4201 ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag gacggggatg
     4261 ggacgataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag aaccccacag
     4321 aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc acattcgact
     4381 tccctgagtt cctgacgatg atggcaagaa aaatgaatga cacagacagt gaagaggaaa
     4441 ttagagaagc gttccgcgtg tttgataagg acggcaatgg ctacatcggc gcagcagagc
     4501 ttcgccacgt gatgacagac cttggagaga agttaacaga tgaggaggtt gatgaaatga
     4561 tcagggtagc agacatcgat ggggatggtc aggtaaacta cgaagagttt gtacaaatga
     4621 tgacagcgaa gtagaagctt gtgattaacc tcaggtgcag gctgcctatc agaaggtggt
     4681 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatcgatctt tttccctctg
     4741 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     4801 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     4861 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     4921 atgcccatat gtaactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     4981 tgctgaaagc atgctcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5041 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5101 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     5161 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5221 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5281 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5341 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     5401 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5461 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5521 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5581 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     5641 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5701 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5761 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5821 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5881 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5941 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     6001 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6061 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6121 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6181 gattcgcagc gcatcgcctt ctatcgcctt cttg
//
