LOCUS       Exported                5671 bp ds-DNA     circular SYN 05-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 6, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    250..268
                     /locus_tag="HSV\TK\PolyA"
                     /label=HSV\TK\PolyA
     misc_feature    599..1242
                     /locus_tag="ColE1\ORI"
                     /label=ColE1\ORI
     misc_feature    1303..1879
                     /locus_tag="CMVie enhancer promotor"
                     /label=CMVie enhancer promotor
     misc_feature    2213..2331
                     /locus_tag="Rhamnose promotor"
                     /label=Rhamnose promotor
     misc_feature    2332..2334
                     /locus_tag="start transcriptie bacteria"
                     /label=start transcriptie bacteria
                     /note="start transcriptie bacteria"
                     /note="color: #ff8000"
     misc_feature    2394..2396
                     /locus_tag="start protein"
                     /label=start protein
                     /note="start protein"
                     /note="color: #ff8000"
     misc_feature    2406..2423
                     /locus_tag="His tag"
                     /label=His tag
                     /note="His tag"
                     /note="color: #ff8000"
     misc_feature    2475..3170
                     /locus_tag="mScarlet-I"
                     /label=mScarlet-I
                     /note="mScI"
                     /note="color: #ff8000"
     misc_feature    3183..3273
                     /locus_tag="antiFRETlinker"
                     /label=antiFRETlinker
                     /note="antiFRETlinker"
                     /note="color: #ff8000"
     misc_feature    3282..3347
                     /locus_tag="P2A sequence"
                     /label=P2A sequence
                     /note="P2A sequence"
                     /note="color: #ff8000"
     misc_feature    3372..4088
                     /locus_tag="mEGFP"
                     /label=mEGFP
     misc_feature    4392..4439
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
     misc_feature    4500..4528
                     /locus_tag="Bact.\promoter"
                     /label=Bact.\promoter
     misc_feature    4603..4769
                     /locus_tag="SV40\early\promoter"
                     /label=SV40\early\promoter
     misc_feature    4707..4842
                     /locus_tag="SV40\ORI"
                     /label=SV40\ORI
     misc_feature    join(4891..5671,1..14)
                     /locus_tag="KanV\Neo\resistance"
                     /label=KanV\Neo\resistance
ORIGIN
        1 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
       61 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
      121 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
      181 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
      241 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
      301 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
      361 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
      421 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
      481 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
      541 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
      601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
      661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
      781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      901 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1261 tgctcacatg ttctttcctg cgttatcccc tgaggccggc ctattaatag taatcaatta
     1321 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1381 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1441 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1501 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     1561 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     1621 cttggcagta catctacgta ttagtcatcg ctattaccat gctgatgcgg ttttggcagt
     1681 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1741 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     1801 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1861 gacgtcgttt agtgaaccgt cagatcacta gatgctttat tgcggtagtt tatcacagtt
     1921 aaattgctaa cgccagtctc gaacttaacg tgcagaagtt ggtcgtgagg cactgggcag
     1981 gtaagtatcg ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
     2041 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2101 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2161 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgaa atcaccacaa
     2221 ttcagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaaag gcccattttc
     2281 ctgtcagtaa cgagaaggtc gcgaattcag gcgcttttta gactggtcgt agggagacca
     2341 caacggtttc cctctagaaa taattttgtt taactataag aaggagatat accatgcggg
     2401 gttctcatca tcaccaccat cacagcagcg gcctggtgcc gcgcggcagc tcgctagcgc
     2461 tgccggtcgc caccatggtg agcaagggcg aggcagtgat caaggagttc atgcggttca
     2521 aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     2581 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     2641 ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc ttcatcaagc
     2701 accccgccga catccccgac tactataagc agtccttccc cgagggcttc aagtgggagc
     2761 gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc tccctggagg
     2821 acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct gacggccccg
     2881 taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac cccgaggacg
     2941 gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggcggc cgctacctgg
     3001 cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc gcctacaacg
     3061 tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg gaacagtacg
     3121 aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag tcgaccagat
     3181 ctctggccga ggcggcagct aaggaggccg cggcgaagga ggcagccgcg aaagaagcag
     3241 ccgcaaagga agcggccgcc aaggctgctg caagatctct cggatcgggt gctactaact
     3301 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggaccttcg ctagcgctgg
     3361 atcccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3421 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3481 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3541 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3601 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3661 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3721 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3781 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     3841 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     3901 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3961 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     4021 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4081 tgtacaagta aaagcttgtg attaacctca ggtgcaggct gcctatcaga aggtggtggc
     4141 tggtgtggcc aatgccctgg ctcacaaata ccactgagat cgatcttttt ccctctgcca
     4201 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     4261 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     4321 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     4381 cccatatgta actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     4441 tgaaagcatg ctcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4501 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4561 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     4621 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4681 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4741 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4801 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     4861 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     4921 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     4981 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5041 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     5101 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5161 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5221 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5281 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5341 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5401 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     5461 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5521 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5581 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5641 tcgcagcgca tcgccttcta tcgccttctt g
//
