LOCUS       Exported                5689 bp ds-DNA     circular SYN 19-JUL-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 6, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    645..1022
                     /locus_tag="4xMTS"
                     /label=4xMTS
                     /note="4xMTS"
     misc_feature    1080..2342
                     /locus_tag="G-Ca-FLITS"
                     /label=G-Ca-FLITS
     misc_feature    1080..1151
                     /locus_tag="M13"
                     /label=M13
     misc_feature    1152..1160
                     /locus_tag="L1"
                     /label=L1
     gene            1161..1892
                     /locus_tag="cp150 F146Y T203Y A D mTQ2"
                     /label=cp150 F146Y T203Y A D mTQ2
     misc_feature    1893..1898
                     /locus_tag="L2"
                     /label=L2
     misc_feature    1899..2342
                     /locus_tag="CaM"
                     /label=CaM
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatgtcc gtcctgacgc
      661 cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg cgcgccaaga
      721 tccattcgtt gggggatccc atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag
      781 gctcggcccg gcggctccca gtgccgcgcg ccaagatcca ttcgttgggg aagcttatgt
      841 ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc
      901 cgcgcgccaa gatccattcg ttgggggatc ccatgtccgt cctgacgccg ctgctgctgc
      961 ggggcttgac aggctcggcc cggcggctcc cagtgccgcg cgccaagatc cattcgttgg
     1021 ggaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca
     1081 tggtcgactc ttcacgtcgt aagtggaata aggcaggtca cgcagtcaga gctataggtc
     1141 ggctgagctc acccgtggct gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     1201 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     1261 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     1321 accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctactggagt
     1381 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtaccagggt ggcagcggtg
     1441 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1501 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     1561 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     1621 ccctcgtgac caccctgtcc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga
     1681 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     1741 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1801 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1861 acaagctgga gtacaactac tatagcgacg atacgcgtga ccaactgact gaagagcaga
     1921 tcgcagaatt taaagaggct ttctccctat ttgacaagga cggggatggg acgataacaa
     1981 ccaaggagct ggggacggtg atgcggtctc tggggcagaa ccccacagaa gcagagctgc
     2041 aggacatgat caatgaagta gatgccgacg gtgacggcac attcgacttc cctgagttcc
     2101 tgacgatgat ggcaagaaaa atgaatgaca cagacagtga agaggaaatt agagaagcgt
     2161 tccgcgtgtt tgataaggac ggcaatggct acatcggcgc agcagagctt cgccacgtga
     2221 tgacagacct tggagagaag ttaacagatg aggaggttga tgaaatgatc agggtagcag
     2281 acatcgatgg ggatggtcag gtaaactacg aagagtttgt ccaaatgatg acagcgaagt
     2341 aggtgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     2401 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2461 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2521 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2581 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     2641 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     2701 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     2761 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     2821 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     2881 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     2941 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3001 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3061 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3121 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3181 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     3241 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3301 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3361 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3421 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3481 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3541 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     3601 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     3661 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     3721 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     3781 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     3841 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     3901 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     3961 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     4021 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     4081 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     4141 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     4201 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     4261 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     4321 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     4381 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     4441 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     4501 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     4561 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     4621 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     4681 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     4741 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     4801 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     4861 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4921 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4981 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5041 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5101 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5161 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5221 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5281 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5341 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5401 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5461 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5521 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5581 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5641 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
