LOCUS       Exported                5363 bp ds-DNA     circular SYN 01-SEP-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 6, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    581..673
                     /locus_tag="ER"
                     /label=ER
                     /note="ER"
     misc_feature    689..1948
                     /locus_tag="G-Ca-FLITS"
                     /label=G-Ca-FLITS
     misc_feature    689..760
                     /locus_tag="M13"
                     /label=M13
     misc_feature    761..769
                     /locus_tag="L1"
                     /label=L1
     gene            770..1501
                     /locus_tag="cp150 F146Y T203Y A D mTQ2"
                     /label=cp150 F146Y T203Y A D mTQ2
     misc_feature    1502..1507
                     /locus_tag="L2"
                     /label=L2
     misc_feature    1508..1948
                     /locus_tag="CaM"
                     /label=CaM
     misc_feature    1955..1966
                     /locus_tag="KDEL"
                     /label=KDEL
                     /note="ER"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attcgccacc atgaggtctt tgctaatctt
      601 ggtgctttgc ttcctgcccc tggctgctct ggggaaagtc tttggacgat gtgagctggc
      661 agcggctatg aagccaccgg tcgccaccat ggtcgactct tcacgtcgta agtggaataa
      721 ggcaggtcac gcagtcagag ctataggtcg gctgagctca cccgtggctg tctatatcac
      781 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
      841 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      901 gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga
      961 gaagcgcgat cacatggtcc tactggagtt cgtgaccgcc gccgggatca ctctcggcat
     1021 ggacgagctg taccagggtg gcagcggtgg catggtgagc aagggcgagg agctgttcac
     1081 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1141 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1201 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca
     1261 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1321 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1381 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1441 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactact atagcgacga
     1501 tacgcgtgac caactgactg aagagcagat cgcagaattt aaagaggctt tctccctatt
     1561 tgacaaggac ggggatggga cgataacaac caaggagctg gggacggtga tgcggtctct
     1621 ggggcagaac cccacagaag cagagctgca ggacatgatc aatgaagtag atgccgacgg
     1681 tgacggcaca ttcgacttcc ctgagttcct gacgatgatg gcaagaaaaa tgaatgacac
     1741 agacagtgaa gaggaaatta gagaagcgtt ccgcgtgttt gataaggacg gcaatggcta
     1801 catcggcgca gcagagcttc gccacgtgat gacagacctt ggagagaagt taacagatga
     1861 ggaggttgat gaaatgatca gggtagcaga catcgatggg gatggtcagg taaactacga
     1921 agagtttgtc caaatgatga cagcgaaggt gtacaaggat gaattgtaag cggccgcgac
     1981 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     2041 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     2101 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2161 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa
     2221 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     2281 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     2341 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     2401 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     2461 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     2521 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     2581 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     2641 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     2701 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2761 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     2821 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     2881 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2941 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     3001 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3061 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     3121 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     3181 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     3241 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     3301 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     3361 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     3421 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     3481 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     3541 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     3601 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     3661 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     3721 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     3781 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     3841 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     3901 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     3961 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     4021 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     4081 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     4141 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     4201 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     4261 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     4321 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     4381 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     4441 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4501 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4561 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4621 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4681 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4741 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4801 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4861 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4921 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4981 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5041 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5101 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5161 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5221 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5281 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
     5341 catagcccat atatggagtt ccg
//
