LOCUS       Exported                6215 bp ds-DNA     circular SYN 05-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 6, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    250..268
                     /locus_tag="HSV\TK\PolyA"
                     /label=HSV\TK\PolyA
     misc_feature    599..1242
                     /locus_tag="ColE1\ORI"
                     /label=ColE1\ORI
     misc_feature    1303..1879
                     /locus_tag="CMVie enhancer promotor"
                     /label=CMVie enhancer promotor
     misc_feature    2213..2331
                     /locus_tag="Rhamnose promotor"
                     /label=Rhamnose promotor
     misc_feature    2332..2334
                     /locus_tag="start transcriptie bacteria"
                     /label=start transcriptie bacteria
                     /note="start transcriptie bacteria"
                     /note="color: #ff8000"
     misc_feature    2394..2396
                     /locus_tag="start protein"
                     /label=start protein
                     /note="start protein"
                     /note="color: #ff8000"
     misc_feature    2406..2423
                     /locus_tag="6xH-tag"
                     /label=6xH-tag
                     /note="6xH-tag"
                     /note="color: #ff8000"
     misc_feature    2433..2450
                     /locus_tag="Thrombine recognition peptide sequence"
                     /label=Thrombine recognition peptide sequence
                     /note="Thrombine recognition peptide sequence"
                     /note="color: #ff8000"
     misc_feature    2475..3191
                     /locus_tag="mTurquoise2"
                     /label=mTurquoise2
                     /note="mTurquoise2"
                     /note="color: #ff8000"
     misc_feature    3204..3294
                     /locus_tag="antiFRET linker"
                     /label=antiFRET linker
                     /note="antiFRET linker"
                     /note="color: #ff8000"
     misc_feature    3303..3368
                     /locus_tag="P2A sequence"
                     /label=P2A sequence
                     /note="bond skipped between G and P"
                     /note="color: #ff8000"
     misc_feature    3393..3479
                     /locus_tag="CaM bindingpeptide"
                     /label=CaM bindingpeptide
     misc_feature    3489..4184
                     /locus_tag="phusionRedVar"
                     /label=phusionRedVar
     misc_feature    4194..4631
                     /locus_tag="CaM"
                     /label=CaM
     misc_feature    4935..4982
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
     misc_feature    5043..5071
                     /locus_tag="Bact.\promoter"
                     /label=Bact.\promoter
     misc_feature    5146..5313
                     /locus_tag="SV40\early\promoter"
                     /label=SV40\early\promoter
     misc_feature    5251..5386
                     /locus_tag="SV40\ORI"
                     /label=SV40\ORI
     misc_feature    join(5435..6215,1..14)
                     /locus_tag="KanV\Neo\resistance"
                     /label=KanV\Neo\resistance
ORIGIN
        1 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
       61 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
      121 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
      181 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
      241 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
      301 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
      361 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
      421 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
      481 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
      541 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
      601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
      661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
      781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      901 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1261 tgctcacatg ttctttcctg cgttatcccc tgaggccggc ctattaatag taatcaatta
     1321 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1381 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1441 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1501 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     1561 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     1621 cttggcagta catctacgta ttagtcatcg ctattaccat gctgatgcgg ttttggcagt
     1681 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1741 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     1801 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1861 gacgtcgttt agtgaaccgt cagatcacta gatgctttat tgcggtagtt tatcacagtt
     1921 aaattgctaa cgccagtctc gaacttaacg tgcagaagtt ggtcgtgagg cactgggcag
     1981 gtaagtatcg ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
     2041 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2101 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2161 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgaa atcaccacaa
     2221 ttcagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaaag gcccattttc
     2281 ctgtcagtaa cgagaaggtc gcgaattcag gcgcttttta gactggtcgt agggagacca
     2341 caacggtttc cctctagaaa taattttgtt taactataag aaggagatat accatgcggg
     2401 gttctcatca tcaccaccat cacagcagcg gcctggtgcc gcgcggcagc tcgctagcgc
     2461 tgccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2521 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2581 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2641 tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc
     2701 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2761 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2821 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2881 acatcctggg gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg
     2941 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     3001 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3061 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     3121 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3181 agctgtacaa gtcgaccaga tctctggccg aggcggcagc taaggaggcc gcggcgaagg
     3241 aggcagccgc gaaagaagca gccgcaaagg aagcggccgc caaggctgct gcaagatctc
     3301 tcggatcggg tgctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
     3361 ctggaccttc gctagcgctg gatcccgcca caatgggcag cgtgaagctg atccccagcc
     3421 tgaccaccgt gatcctcgtg aagtccatgc tgcggaagcg gagcttcggc aaccccttca
     3481 agtataatac ggagaccctg taccccgctg acggcggcct ggaaggcgca tgtgacatgg
     3541 ccctgaagct cgtgggcggg ggccacctga actgcagcct tgagaccaca tacagatcca
     3601 agaaacccgc tacgaacctc aagatgcccg gtgtctacaa cgtggaccac agactggaac
     3661 gaatcaaaga ggccgacgat gagacctacg tcgagctgca cgaggtggct gtggccagat
     3721 acgtgggcct gggtggtggc ggaggtacag gcgggagtgt gagcgagctg attaaggaga
     3781 acatgccaat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     3841 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtcgtcgagg
     3901 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcagaa
     3961 ccttcatcaa gcaccctcct ggcatccccg acttctttaa gcagtccttc cctgagggct
     4021 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     4081 ccagcctcca ggacggctgc ctcatctaca acgtcaaggt tagagggatg aacttcccag
     4141 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccagtaat ggccaactga
     4201 ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag gacggggatg
     4261 ggacgataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag aaccccacag
     4321 aagcggagct gcaggacatg atcaatgaag tagatgccga cggtgacggc acattcgact
     4381 tccctgagtt cctgacgatg atggcaagaa aaatgagtta tagagtcact gaagaggaaa
     4441 ttagagaagc gttccgcgtg tttgataagg acggcaatgg ctacatcggc gcagcagagc
     4501 ttcgccacgt gatgacagac cttggagaga agttaacaga tgaggaggtt gatgaaatga
     4561 tcagggtagc agacatcgat ggggatggtc aggtaaacta cgaagagttt gtacaaatga
     4621 tgacagcgaa gtagaagctt gtgattaacc tcaggtgcag gctgcctatc agaaggtggt
     4681 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatcgatctt tttccctctg
     4741 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     4801 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     4861 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     4921 atgcccatat gtaactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     4981 tgctgaaagc atgctcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5041 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5101 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagaggtgt
     5161 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5221 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5281 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5341 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5401 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     5461 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5521 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5581 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     5641 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5701 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5761 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5821 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5881 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5941 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6001 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6061 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6121 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6181 cgattcgcag cgcatcgcct tctatcgcct tcttg
//
