LOCUS       Exported                6239 bp ds-DNA     circular SYN 05-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 6, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    250..268
                     /locus_tag="HSV\TK\PolyA"
                     /label=HSV\TK\PolyA
     misc_feature    599..1242
                     /locus_tag="ColE1\ORI"
                     /label=ColE1\ORI
     misc_feature    1303..1879
                     /locus_tag="CMVie enhancer promotor"
                     /label=CMVie enhancer promotor
     misc_feature    2213..2331
                     /locus_tag="Rhamnose promotor"
                     /label=Rhamnose promotor
     misc_feature    2332..2334
                     /locus_tag="start transcriptie bacteria"
                     /label=start transcriptie bacteria
                     /note="start transcriptie bacteria"
                     /note="color: #ff8000"
     misc_feature    2394..2396
                     /locus_tag="start protein"
                     /label=start protein
                     /note="start protein"
                     /note="color: #ff8000"
     misc_feature    2406..2423
                     /locus_tag="6xH-tag"
                     /label=6xH-tag
                     /note="6xH-tag"
                     /note="color: #ff8000"
     misc_feature    2433..2450
                     /locus_tag="Thrombine recognition peptide sequence"
                     /label=Thrombine recognition peptide sequence
                     /note="Thrombine recognition peptide sequence"
                     /note="color: #ff8000"
     misc_feature    2475..3191
                     /locus_tag="mTurquoise2"
                     /label=mTurquoise2
                     /note="mTurquoise2"
                     /note="color: #ff8000"
     misc_feature    3204..3294
                     /locus_tag="antiFRET linker"
                     /label=antiFRET linker
                     /note="antiFRET linker"
                     /note="color: #ff8000"
     misc_feature    3303..3368
                     /locus_tag="P2A sequence"
                     /label=P2A sequence
                     /note="bond skipped between G and P"
                     /note="color: #ff8000"
     CDS             3393..3464
                     /codon_start=1
                     /locus_tag="calmodulin-binding peptide"
                     /product="calmodulin-binding peptide from avian smooth
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="MVDSSRRKWNKAGHAVRAIGRLSS"
     misc_feature    3480..4199
                     /locus_tag="cpRFP"
                     /label=cpRFP
     misc_feature    4215..4655
                     /locus_tag="CaM"
                     /label=CaM
     misc_feature    4959..5006
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
     misc_feature    5067..5095
                     /locus_tag="Bact.\promoter"
                     /label=Bact.\promoter
     misc_feature    5170..5337
                     /locus_tag="SV40\early\promoter"
                     /label=SV40\early\promoter
     misc_feature    5275..5410
                     /locus_tag="SV40\ORI"
                     /label=SV40\ORI
     misc_feature    join(5459..6239,1..14)
                     /locus_tag="KanV\Neo\resistance"
                     /label=KanV\Neo\resistance
ORIGIN
        1 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
       61 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
      121 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
      181 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
      241 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
      301 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
      361 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
      421 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
      481 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
      541 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
      601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
      661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
      781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      901 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1261 tgctcacatg ttctttcctg cgttatcccc tgaggccggc ctattaatag taatcaatta
     1321 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1381 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1441 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1501 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     1561 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     1621 cttggcagta catctacgta ttagtcatcg ctattaccat gctgatgcgg ttttggcagt
     1681 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1741 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     1801 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1861 gacgtcgttt agtgaaccgt cagatcacta gatgctttat tgcggtagtt tatcacagtt
     1921 aaattgctaa cgccagtctc gaacttaacg tgcagaagtt ggtcgtgagg cactgggcag
     1981 gtaagtatcg ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
     2041 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2101 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2161 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgaa atcaccacaa
     2221 ttcagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaaag gcccattttc
     2281 ctgtcagtaa cgagaaggtc gcgaattcag gcgcttttta gactggtcgt agggagacca
     2341 caacggtttc cctctagaaa taattttgtt taactataag aaggagatat accatgcggg
     2401 gttctcatca tcaccaccat cacagcagcg gcctggtgcc gcgcggcagc tcgctagcgc
     2461 tgccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2521 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2581 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2641 tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc
     2701 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2761 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2821 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2881 acatcctggg gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg
     2941 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     3001 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3061 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     3121 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3181 agctgtacaa gtcgaccaga tctctggccg aggcggcagc taaggaggcc gcggcgaagg
     3241 aggcagccgc gaaagaagca gccgcaaagg aagcggccgc caaggctgct gcaagatctc
     3301 tcggatcggg tgctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
     3361 ctggaccttc gctagcgctg gatcccgcca caatggtcga ctcatcacgt cgtaagtgga
     3421 ataaggcagg tcatgcagtc agagctatag gtcggctgag ctcacttgag tcgctgctgt
     3481 ccaccgagcg gctttacccc gaggacggcg ctctgaaggg cgagatcaag cagaggctga
     3541 agctgaagga cggcggccac tacgccgccg aggtcaagac cacctacaag gccaagaagc
     3601 ccgtgcagct gcccgacgcc tacatcgtcg acatcaagtt ggacatcgtg tcccacaacg
     3661 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     3721 tggacgagct gtacaagggt ggcactggcg gttccatggt gagcaagggc gtggaggata
     3781 acatggcctt catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtcaacg
     3841 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     3901 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     3961 agttcatgta cggctccaag gcctacatta agcacccagc cgacatcccc gactactgga
     4021 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggaggtg
     4081 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     4141 tgcgcggcac caacttcccc tccaacggcc ccgtaatgca gaagaagacc atgggctggc
     4201 tgggcggtac gcgtgaccaa ctgactgaag agcagatcgc agaatttaaa gaggctttct
     4261 ccctatttga caagggcggg gctgggacga taacaaccaa ggagctgggg acggtgatgc
     4321 ggtctctggg gcagaacccc acagaagcag agctgcagga catgatcaat gaagtagatg
     4381 ccgacggtga cggcacatta gacttccctg agttcctgac gatgatggca agaaaaatga
     4441 atgactcaga cagtgaagag gaaattagag aagcgttccg tgtgtttgat aaggacggca
     4501 atggctacat cggcgcagca gagcttcgcc acgtgatgac aaaccttgga gagaagttaa
     4561 cagatgaaga ggttgatgaa atgatcaggg tagcagacat cgatggggat ggtcaggtaa
     4621 actacgaaga gtttgtacaa atgatgacag cgaagtagaa gcttgtgatt aacctcaggt
     4681 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     4741 ctgagatcga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     4801 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     4861 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     4921 atttggttta gagtttggca acatatgccc atatgtaact agcataaccc cttggggcct
     4981 ctaaacgggt cttgaggggt tttttgctga aagcatgctc ggggaaatgt gcgcggaacc
     5041 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5101 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
     5161 ggaatgtgtg tcagttagag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5221 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5281 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5341 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5401 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     5461 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     5521 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5581 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     5641 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5701 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5761 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5821 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5881 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5941 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     6001 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6061 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     6121 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     6181 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttg
//
