LOCUS       Exported                5873 bp ds-DNA     circular SYN 02-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 6, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pDRESS     6556 bp    DNA   circular 30-DEC-2016 
            DEFINITION  pDRESS_mTQ2_mScarlet. SOURCE ORGANISM
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="synthetic DNA construct"
                     /label=pDRESS
                     /note="color: #000000"
                     /note="/locus_tag=pDRESS"
     promoter        1021..1597
                     /locus_tag="CMVie enhancer promoter"
                     /label=CMVie enhancer promoter
                     /note="CMVie enhancer promoter"
                     /note="color: #8080ff"
     misc_feature    1598..1601
                     /locus_tag="start vertebrate transcription"
                     /label=start vertebrate transcription
                     /note="start vertebrate transcription"
                     /note="color: #ff8000"
     misc_feature    1931..2049
                     /locus_tag="Rhamnose promotor"
                     /label=Rhamnose promotor
                     /note="Rhamnose promotor"
                     /note="color: #ff8000"
     primer_bind     2031..2053
                     /locus_tag="forward sequence primer -
                     GCTTTTTAGACTGGTCGTAGGGA"
                     /label=forward sequence primer -
                     GCTTTTTAGACTGGTCGTAGGGA
                     /label=forward sequence primer - GCTTTTTAGACTGGTCGTAGGGA
                     /note="color: black; sequence: GCTTTTTAGACTGGTCGTAGGGA"
     misc_feature    2050..2052
                     /locus_tag="start transcriptie bacteria"
                     /label=start transcriptie bacteria
                     /note="start transcriptie bacteria"
                     /note="color: #ff8000"
     misc_feature    2112..2114
                     /locus_tag="start protein"
                     /label=start protein
                     /note="start protein"
                     /note="color: #ff8000"
     misc_feature    2124..2141
                     /locus_tag="6xH-tag"
                     /label=6xH-tag
                     /note="6xH-tag"
                     /note="color: #ff8000"
     misc_feature    2151..2168
                     /locus_tag="Thrombine recognition peptide sequence"
                     /label=Thrombine recognition peptide sequence
                     /note="Thrombine recognition peptide sequence"
                     /note="color: #ff8000"
     misc_feature    2193..2909
                     /locus_tag="mTurquoise2"
                     /label=mTurquoise2
                     /note="mTurquoise2"
                     /note="color: #ff8000"
     misc_feature    2922..3012
                     /locus_tag="antiFRET linker"
                     /label=antiFRET linker
                     /note="antiFRET linker"
                     /note="color: #ff8000"
     misc_feature    3021..3086
                     /locus_tag="P2A sequence"
                     /label=P2A sequence
                     /note="bond skipped between G and P"
                     /note="color: #ff8000"
     primer_bind     complement(3190..3211)
                     /locus_tag="RV sequence primer"
                     /label=RV sequence primer
                     /note="color: black; sequence: CGATCTCAGTGGTATTTGTGAG"
     polyA_signal    3211..3417
                     /locus_tag="Rabbit B-Globin terminator"
                     /label=Rabbit B-Globin terminator
                     /note="Rabbit B-Globin terminator"
                     /note="color: #8000ff"
     terminator      3421..3468
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
                     /note="T7 terminator"
                     /note="color: #000000"
     rep_origin      complement(4381..4991)
                     /direction=LEFT
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="pBR322_origin "
                     /note="color: #c0c0c0"
     misc_feature    complement(4996..5811)
                     /locus_tag="Kanamycin resistance"
                     /label=Kanamycin resistance
                     /note="Kanamycin resistance"
                     /note="color: #ff8000; direction: LEFT"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tataggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttat acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggcct
     1021 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     1081 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1141 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1201 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
     1261 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1321 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgc
     1381 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
     1441 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1501 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
     1561 gggaggtcta tataagcaga cgtcgtttag tgaaccgtca gatcactaga tgctttattg
     1621 cggtagttta tcacagttaa attgctaacg ccagtctcga acttaacgtg cagaagttgg
     1681 tcgtgaggca ctgggcaggt aagtatcggg ccctttgtgc ggggggagcg gctcggggct
     1741 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     1801 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     1861 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggatc
     1921 ggaccgaaat caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt
     1981 tgccaaaggc ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga
     2041 ctggtcgtag ggagaccaca acggtttccc tctagaaata attttgttta actataagaa
     2101 ggagatatac catgcggggt tctcatcatc accaccatca cagcagcggc ctggtgccgc
     2161 gcggcagctc gctagcgctg ccggtcgcca ccatggtgag caagggcgag gagctgttca
     2221 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2281 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2341 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc tggggcgtgc
     2401 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2461 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2521 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2581 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac tttagcgaca
     2641 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     2701 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2761 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
     2821 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2881 tcactctcgg catggacgag ctgtacaagt cgaccagatc tctggccgag gcggcagcta
     2941 aggaggccgc ggcgaaggag gcagccgcga aagaagcagc cgcaaaggaa gcggccgcca
     3001 aggctgctgc aagatctctc ggatcgggtg ctactaactt cagcctgctg aagcaggctg
     3061 gagacgtgga ggagaaccct ggaccttcgc tagcgctacc ggtgcctcaa catgatgtac
     3121 aagtaagtga ttaacctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
     3181 atgccctggc tcacaaatac cactgagatc gatctttttc cctctgccaa aaattatggg
     3241 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     3301 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     3361 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgtaa
     3421 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaagcatgc
     3481 ggaggaaatt ctccttgaag tttccctggt gttcaaagta aaggagtttg caccagacgc
     3541 acctctgttc actggtccgg cgtattaaaa cacgatacat tgttattagt acatttatta
     3601 agcgctagat tctgtgcgtt gttgatttac agacaattgt tgtacgtatt ttaataattc
     3661 attaaattta taatctttag ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc
     3721 tttatatctg aatttaaata ttaaatcctc aatagatttg taaaataggt ttcgattagt
     3781 ttcaaacaag ggttgttttt ccgaaccgat ggctggacta tctaatggat tttcgctcaa
     3841 cgccacaaaa cttgccaaat cttgtagcag caatctagct ttgtcgatat tcgtttgtgt
     3901 tttgttttgt aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg tatttctttc
     3961 atcactgtcg ttagtataca attgactcga cgtaaacacg ttaaatagag cttggacata
     4021 tttaacatcg ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc
     4081 gttagaagtt gcttccgaag acgattttgc catagccaca cgacgcctat taattgtgtc
     4141 ggctaacacg tccgcgatca aatttgtagt tgagcttttt ggaattgcga tcgcataact
     4201 tcgtatagca tacattatac gaagttataa gctcggaacg ctgcgctcgg tcgttcggct
     4261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4381 cgcgttgctg gcgtttttcc ataggctccg ccccctgacg agcatcacaa aaatcgacgc
     4441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4561 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     4621 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4741 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4801 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4861 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4921 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4981 caagaagatc ctttgttaga aaaactcatc gagcatcaag tgaaactgca atttattcat
     5041 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     5101 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     5161 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     5221 accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac
     5281 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcaccaa ccaaaccgtt
     5341 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgccgttaa aaggacaatt
     5401 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     5461 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt
     5521 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     5581 ttccgtcagc cagtttagcc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     5641 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
     5701 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     5761 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca tactcttcct
     5821 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgtccgcgc gtt
//
