LOCUS       Exported                8245 bp ds-DNA     linear   UNA 06-SEP-2022
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 15, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     LTR             1..413
                     /locus_tag="5'LTR"
                     /label=5'LTR
                     /note="/vntifkey=19"
     misc_feature    464..601
                     /locus_tag="psi"
                     /label=psi
                     /note="/vntifkey=21"
     misc_feature    568..932
                     /locus_tag="gag"
                     /label=gag
                     /note="/vntifkey=21"
     misc_feature    1078..1319
                     /locus_tag="RRE"
                     /label=RRE
                     /note="/vntifkey=21"
     CDS             1707..1802
                     /locus_tag="Hygro"
                     /label=Hygro
                     /note="/vntifkey=4"
     misc_feature    1847..1964
                     /locus_tag="cPPT"
                     /label=cPPT
                     /note="/vntifkey=21"
     promoter        2023..2604
                     /locus_tag="CMV"
                     /label=CMV
                     /note="/vntifkey=29"
     misc_feature    2617..2937
                     /locus_tag="FKBP (no linker)"
                     /label=FKBP (no linker)
     misc_feature    2938..2967
                     /locus_tag="cpGFP(1)"
                     /label=cpGFP
                     /label=cpGFP(1)
     misc_feature    2938..2967
                     /locus_tag="GS Linker 2"
                     /label=GS Linker 2
     misc_feature    2974..3711
                     /locus_tag="cpGFP"
                     /label=cpGFP
     misc_feature    3697..3711
                     /locus_tag="linker"
                     /label=linker
     misc_feature    3706..3711
                     /locus_tag="ECORV"
                     /label=ECORV
     misc_feature    3712..4083
                     /locus_tag="OPRK nanobody"
                     /label=OPRK nanobody
     misc_feature    4102..4690
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="/vntifkey=21"
     LTR             4761..4996
                     /locus_tag="3'\LTR\SIN"
                     /label=3' LTR SIN
                     /label=3'\LTR\SIN
                     /note="/vntifkey=19"
     rep_origin      5550..5980
                     /locus_tag="F1\ORI"
                     /label=F1 ORI
                     /label=F1\ORI
                     /note="/vntifkey=33"
     misc_feature    6351..7157
                     /locus_tag="AMP\r"
                     /label=AMP r
                     /label=AMP\r
                     /note="/vntifkey=21"
     rep_origin      complement(7283..7898)
                     /direction=LEFT
                     /locus_tag="pUC\Origin"
                     /label=pUC Origin
                     /label=pUC\Origin
                     /note="/vntifkey=33"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagagcg ctgccgtctg gaccgatggc tgtgtggaag
     1741 tgctcgccga tagtggaaac agacgcccca gcactcgtcc tagggcaaag gatctgcagt
     1801 aatgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     1861 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1921 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1981 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     2041 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2101 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2161 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2221 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2281 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2341 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2401 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2461 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2521 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2581 gcagagctct ctggctaact gtcgggatcc accatgggag tgcaggtgga aaccatctcc
     2641 ccaggagacg ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg
     2701 atgcttgaag atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt
     2761 atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg
     2821 ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca
     2881 ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaaggc
     2941 ggcagtggca gtagtggcgg cagtggctct agaaacgtct atatcaaggc cgacaagcag
     3001 aagaacggca tcaaggcgaa cttccacatc cgccacaaca tcgaggacgg cggcgtgcag
     3061 ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3121 aaccactacc tgagcgtgca gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac
     3181 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3241 aagggcggta ccggagggag catggtgagc aagggcgagg agctgttcac cggggtggtg
     3301 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3361 ggtgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3421 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3481 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3541 atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3601 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3661 gacggcaaca tcctggggca caagctggag tacaacctta aggaagatat catggctcaa
     3721 gtccaacttg tagagtctgg aggaggtctc gtgcggcccg gcggtagcct gcgactctcc
     3781 tgtgttgatt cagagaggac gagctaccct atggggtggt ttagacgggc acccggaaag
     3841 gagcgcgaat tcgttgcaag tatcacgtgg agtggcattg acccgactta tgctgatagc
     3901 gttgccgacc ggtttactac gtccagagac gttgcgaata acacgctgta cctccagatg
     3961 aactctctca agcacgaaga tacagcggtc tattactgtg ctgcaagagc acccgtcggt
     4021 caatcttcct ctccttatga ttatgactat tggggacagg ggactcaggt aactgtgtcc
     4081 agttagacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     4141 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4201 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4261 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4321 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4381 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4441 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     4501 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4561 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4621 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4681 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     4741 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     4801 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4861 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4921 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4981 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     5041 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     5101 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5161 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     5221 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5281 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5341 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     5401 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     5461 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     5521 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     5581 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5641 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5701 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5761 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5821 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5881 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5941 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6001 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     6061 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6121 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6181 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6241 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6301 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6361 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6421 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6481 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6541 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6601 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6661 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6721 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6781 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6841 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6901 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6961 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7021 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7081 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7141 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7201 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7261 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7321 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7381 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7441 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7561 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7801 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7861 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7921 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7981 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     8041 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     8101 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     8161 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     8221 gggaacaaaa gctggagctg caagc
//
