LOCUS       Exported               15712 bp DNA     circular SYN 19-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 15712)
  AUTHORS   Jessica Nelson
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2022 from SnapGene 5.3.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15712
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     polyA_signal    1057..1178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1301..2011)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     regulatory      2008..2017
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     2417..2436
                     /label=157
     primer_bind     2659..2675
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2695..2715
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2717..2735
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2755..2769
                     /label=Homology for primer 213 here
     primer_bind     2761..2777
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        2802..8533
                     /label=myo6b
     primer_bind     complement(3350..3369)
                     /label=158a
     primer_bind     8232..8251
                     /label=159a
     CDS             8528..8539
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(8584..8602)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    8602..8626
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     8694..8721
                     /label=RJ0305
                     /note="RJ0305"
     CDS             8698..11535
                     /codon_start=1
                     /label=CaSR
                     /note="CaSR"
                     /translation="MRFHLKFYLHYLVLLGSSCVISTYGPNQRAQKTGDILLGGLFPMH
                     FGVASKDQDLAARPESTECVRYNFRGFRWLQSMIFAIEEINNSSTLLPNITLGYRIFDT
                     CNTVSKALEASLSFVAQNKIDSLNLDEFCNCTGNIPSTIAVVGASGSAVSTAVADLLGL
                     FYIPQISYASSSRLLSNKNQYKSFMRTIPTDEYQAIAMAAIIEHFQWNWVIAIASDDEY
                     GRPGIEKFENEMFHRDICIDLNVLISQYVDEAEIRRLADRIQNSSAKVIVVFASGPDIE
                     PLVKEMVRRNITDRVWLASEAWASSSLVAKPEYLDVMGGTIGFALRAGHIPGFKDFLQQ
                     VHPKKSSHNEFVREFWEETFNCYLEDSPRNADSENGSTSFRPLCTGEEDIASVETPYLD
                     YTHLRISYNVYVAVYAIAQALQDILTCTPGKGLFSNGSCADIRKVEAWQVLKQLRHLNF
                     IDSMGERVRFDNGSELSANYTIINWHRSPEDGSVVFKEVGYYSIHNKNVAKLSIDRSKI
                     LWNGRLTEVPFSNCSVECEPGTRKGIIDGEPTCCFECTECSDGEYSDHKDASFCVKCPN
                     NSWSNGNHTSCFLKEIEFLSWSEPFGIALALFAVLGVLLTAFVLGVFVQFRDTPIVKAS
                     NRELSFLLLFSLICCFSSSLIFIGEPQDWTCRVRQPAFGISFVLCISCILVKTNRVLLV
                     FEAKIPTSLHRKWWGLNLQFLLVFLFTFVQVMICVVWLYNAPPGSYKNYDIDEIIFITC
                     NEGSMMALGFLIGYTCLLAAICFFFAFKSRKLPENFTEAKFITFSMLIFFIVWISFIPA
                     YFSTYGKFVSAVEVIAILASSFSLLACIFFNKVYIILLKPSRNTIEEVRCSTAAHAFKA
                     AAKATLRHSSGFRKRSSSVGGSSASSPSSSISMKTNGNEMESPSTRRHGSKPRVSFGSG
                     TVSLSLSFEEARNSMK"
     primer_bind     complement(9030..9049)
                     /label=160
     primer_bind     complement(9060..9085)
                     /label=HS CaSR SDMB R1 C133W C135Y
     primer_bind     9086..9106
                     /label=HS CaSR SDMB F1 (two alternate primers)
     primer_bind     9176..9195
                     /label=RJ0319
                     /note="RJ0319"
     primer_bind     9337..9358
                     /label=RJ0320
                     /note="RJ0320"
     primer_bind     9896..9915
                     /label=RJ0321
                     /note="RJ0321"
     variation       10238
                     /label=A>G
                     /note="A>G"
     variation       10468
                     /label=C>G
                     /note="C>G"
     variation       10489
                     /label=A>T
                     /note="A>T"
     primer_bind     10791..10807
                     /label=RJ0323
                     /note="RJ0323"
     primer_bind     10973..10993
                     /label=HS011
     primer_bind     complement(11158..11178)
                     /label=SDM Primer R1 HS
     primer_bind     11179..11201
                     /label=SDM Primer F1 HS
     misc_feature    11189..11190
                     /label=AA for A831E
     primer_bind     11251..11271
                     /label=145
     primer_bind     complement(11320..11342)
                     /label=HS012
     primer_bind     11536..11571
                     /label=RJ0313
                     /note="RJ0313"
     CDS             11536..11550
                     /codon_start=1
                     /label=GGGGA Linker
                     /note="GGGGA Linker"
                     /translation="GGGGA"
     CDS             11551..12267
                     /codon_start=1
                     /transl_table=11
                     /gene="egfp"
                     /product="enhanced green fluorescent protein"
                     /label=EGFP
                     /note="/Contains A206K mutation to ensure monomerization"
                     /db_xref="GI:1377909"
                     /protein_id="AAB02572.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     gene            join(11551..12165,12169..12267)
                     /gene="egfp"
                     /label=egfp
     primer_bind     complement(11657..11674)
                     /label=146
     primer_bind     12171..12190
                     /label=161
     misc_binding    12268..12568
                     /label= GW1
                     /note="GW1 priming site"
     promoter        complement(12286..12303)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(12287..12303)
                     /label=T7
                     /note="T7"
     polyA_signal    12308..12504
                     /label=SV40 Late PolyA
                     /note="SV40 Late PolyA"
     polyA_signal    12308..12442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(12312..12442)
                     /label=SV40 pA
                     /note="SV40 Early polyA"
     terminator      12318..12504
                     /label=SV40 Late PolyA Terminator
                     /note="SV40 Late PolyA Terminator"
     protein_bind    complement(12559..12583)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12609..12629)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(12637..12653)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    12669..12803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13475..13491)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(13528..13546)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13567..13583)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    13591..13607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(13615..13645)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13660..13681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13969..14557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14728..15588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(15589..15693)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggcccattt acgccttaag atacattgat gagtttggac
     1081 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1141 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1201 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1261 aatgtggtat ggctgattat gatctagagt cgcggccgct ttacttgtac agctcgtcca
     1321 tgccgccggt ggagtggtgg ccctcggcgc gctcgtactg ttccacgatg gtgtagtcct
     1381 cgttgtggga ggtgatgtcc agcttgatgt tgacgttgta ggcgccgggc agctgcacgg
     1441 gcttcttggc cttgtaggtg gtcttgacct cggcgtcgta gtggccgccg tccttcagct
     1501 tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg
     1561 aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc
     1621 cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg
     1681 tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
     1741 acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca
     1801 tgaactgagg ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca
     1861 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
     1921 cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg
     1981 ccatgttgtc atcctcgccc ttgctcacca tggtggcgac cggtaatgtc agacctggta
     2041 actccttact gtaacttatt gcacctgatg aacaaaagat gtgaactgaa cgcaagtagg
     2101 aggctaatag aggctagtac agtacaaaga tccccaaatg atgtgtgccg gccttagtgg
     2161 aggcagaccc ctggttatat acctgtgctg cagaaagagg ggcttacaaa tggatctctc
     2221 tggaatggca ttatctcatg gtgggaacag tgttgtcagc agaatcagcg gggtggactc
     2281 tgacctgaga atgacagtct agcactgaat ggctcagaca atactcttgt ggccaactat
     2341 caccggaaaa taagtgcctg ccaaaacaaa ccagaccaga tgaagctaca tggctgtaga
     2401 acgtcagtat ttaatgtgtt gtggccactt ggttcaccag atttaagagc taattttttt
     2461 aaatatcaca gataatcaat cgcttttttt tttcttgact gaaaagctta gactggaaaa
     2521 ttctttgaaa aagtttgcac tacagaaaat aaatacataa catcctgcac tgaatgcaca
     2581 ctattaatag aaaagagagc attattgtag gaaatgtatt gtatatgtac attctgtgca
     2641 tttcacaatt ttctcgagca ggaaacagct atgaccatga ttacgccaag ctatcaactt
     2701 tgtatagaaa agttggtaat acgactcact atagggcgaa ttgggtaccg ggccccccct
     2761 cgaggtcgac ggtatcgata agcttgatat cgaattcctg cagccccggc agttctgtgg
     2821 gtgcgaatgc cttgttgatg gcagaggtca gaggagaatg gccagactgg tttgagctga
     2881 cacacgtcga accttgaggt ggatgggcta cagcagcaga agaccacaca gggtgccact
     2941 cttgtcagct aagaacagct aaatgagatg gaatttagca caggctcacc aaaattggac
     3001 aatagaagat tggaaaaacg tctggtctga cgagtctcga tttctgttgc cattcagatg
     3061 gtagggtcag aatttggcgt caacagcatg aaagtatgga ttcatcctgc tttgtatcaa
     3121 cggttcaggc tggtggtggt gttatggtgt gggggatgtt ttcttggcac acttttggcc
     3181 cattagtacc aattgagcat catgtcaatg tcacagcctt cctccgtatt gttgctgact
     3241 atgtccatcc ttttatgacc acagtgtacc catcttctga tacttgaaca atgagttcac
     3301 agtactcaaa tgacctccag tcaccagctc aatccaatgg agcacctttg ggatgtggtg
     3361 gaatgggaga tttgcatcat gaatgtgcag ttatgatgct atcatgtcaa tatggactaa
     3421 aatatctgag gagtaccttg atgagtttat gccataaagg attaaggcag ttctgaaggc
     3481 aaaagtgtgt ccaacctggt actacagtag taaggtgtac ctaataaagt ggccggtgag
     3541 agtacattac tactctgtaa aaacaaatct tgctgcctta tttttttagt taaatcaaat
     3601 gaacttgtct agcttgcatc caactattaa gttacatttt gtacaacgta aaaaaaaaca
     3661 aaaaaaaaca tgaaaccgga ttagctcatt ttaacaataa tgtaaagtag caaaacattt
     3721 gttttcatca ctttgcttga tttattttta cagtgcaagc attatagact caatttaaag
     3781 acaactttcc ttgatgttta gttatttttt tctgatatcc ctactgaaaa aacaacttta
     3841 accagcctaa gctagttggc tgcttttagc tggttgacta gtctggtttt agaggggttg
     3901 tggccacttc caggctggtt tccagcaatt tccagactcg tcttagctgg tcaggctggg
     3961 agatgactag gtaaaaccag cttgaccagc cagatttaag ctgaacttag ctggttttgg
     4021 ctgggctccc agcctggcta ggctggtcaa gctggtttaa gctggtcatc tcccagcctg
     4081 accagctaag actagtctgg aaattgctgg aaaccagtgg ccaaaaccac tttaaaacct
     4141 gcctggcagc taaaaccagg ctggtttaag caattctttt cagtagggat atttactaaa
     4201 acttgccaaa tcataacaaa acatacatca aagtaacaaa tttattcaac tttcatgtga
     4261 tgtataaatc tcaatgtcta gaaattgaca tttaaaaaaa acatttaaat tataaaattt
     4321 aaacttaaat aaaattgatt tatttttacg tctcgagatc tgatctcaga gcatattcat
     4381 actctagtta tgctggttat tttatgtttt tatttataat gtgtcccagc tggcataacc
     4441 cccttacttc atatgttctc ccattagagc tagtaatata ggctacataa aaaattatta
     4501 ttttaagata atcattttgc tataagaact ctttcatatt ttttgcttct ggcaaatgtg
     4561 ctctggtaca ccttgcatgc tttatgcacg tttgttttta tgtctgcaat gctcttggga
     4621 aatgcagaag tcagcgtggc attaaatcta attaatggag cttcttcagg ctcaccagat
     4681 gacttaaaac tcttgtttag ctgtttctgg ggaaactgat gtcgaatatt aggcctactc
     4741 tgtctccatc ccaagatatt ctgtgagact atacgtaact caagctggag gctatagtta
     4801 acctgtataa caccccaaag caacagcatg aagtcgtata tgggatgaag agtggttgtc
     4861 agagtcttaa acacacacaa aaaaactgtt tgttataact tttttgctta gaaatttgat
     4921 atagcgacac gtagtgagag atcaggtgtc ctaattagat ttacaaacac aaagtctgct
     4981 ttcaatgtgg tttaagattt caacttagga atacttctat acgttttgaa ttacaagcca
     5041 aataaaatat taatgagcct tcagtttgta tcagttgcgc gccttttagt aactatggca
     5101 acccagtagg caccttgtga ctttctcctc atcccttagc aagttttatc catcctgaat
     5161 taaactttac tagtacgtta acgaactggg acttgtttta gtacaataat acagtttata
     5221 acttgaagaa ctgattccag ccaccagaac gttttaactt aaccttcttt tagttcccag
     5281 aacgttcttt taaggttttt ggttagccaa aatgaaaaca acattagctt taacgttagg
     5341 ttgtaaagtg tacacaagac gataataatg tgcttgaggg gataactttt aatattagac
     5401 gacctgtttt agtatgtttg atgtttttat gtgatgataa cactattttt ttacataaca
     5461 ctaataagca gcttgatttg agcgataaga actggtaaaa tttaccatga catactatat
     5521 atttagtcat taaaactaaa atattcctcg taaaacatat tctatatcac ctttatagta
     5581 aacaaccagt tattcgtgtc atttagatca atcaatgtat cggctacgtc ttaaaagatt
     5641 agtttgcgcc ctacctaggc agcataatgg tgcgtcatgt atttgtagtg aagcccgtca
     5701 agtaacttat atcattgtag cgcgtacatg taaagtccac tgcactcgcc tactgcatgc
     5761 cgcttttact caaactattt tgccagtcat gaccctgtgt attgagacac cacctgtgtc
     5821 agtcagcgct actgtgtcac cgttgaggtc gctgttattc cagtggactt cagtctgaag
     5881 ccggacacac agagaggaac gacagcggct ttggaaaagg gaatattcag catcgaggag
     5941 ggaatctgtc accgctaacc gggacagtct gtcgctcatc tgtcctcaaa agaagctgct
     6001 cttaccggat ttacacacaa tgtgagtgac ttcgcttact ttatttattg cttgactgcg
     6061 ctttgtggca actaaaggtg ttgttaggcg taagttgtgt ttaattgctt ctacagtggc
     6121 gcgctagcat gctgctaatg aatgtgttca ttcagtatgg ttacagtgaa ttttgttatg
     6181 tcaatgtttg caatgtcatt tatttttgca gaattttgtt taaaacgcag ggttgtatga
     6241 caacgagttc ggttaacaaa ctactaggaa tacatgtata tcagcgatag ttaaattcat
     6301 tcttaatctt gcttttggtt gtgtttagtt ttttttcttt gagagctttt tctatggtgt
     6361 tttgtaagct gcgtgtgtgt gtgaccaaag ttttccagaa aattagttaa ggcaggcagt
     6421 atgtcatgga aactcgacag gatatcctat gtgtgcagtt ataacgagtg taactttctt
     6481 ctgaagttgt ggaaaccatt gacagaggta acgttagtta tttgggttcc aatcgtatga
     6541 atgatggatg ctttaaacgc tgaattaggt gactgtaaac tgccgtttgt gcgcatgcgc
     6601 gactgctctg gttaaacgtc gtaatggttc cacctttata atcccccaag ggtcattcta
     6661 cgaaatgtgt gtcgtttaaa gcgatttata cttgcattta tgcacacata cagtatgcat
     6721 gacattaatg tggctattgc agctagcttt taaaatgatt ttggtgagag ctttaaaaat
     6781 aaataataat aacaaaactt tacattgcaa ttcattcatt catatattca ttcattcatt
     6841 cattcattca ttcattcatt cattcattgg ccacacttca ttttatggta aaactcttgc
     6901 tattaataaa tcattaattt tgacttgtag tttgacaaac ttctactttg ctgcttatta
     6961 atagtaaggt tttagttggc tttatgtgtt tggtagggtt agggatgcaa aatatgtatt
     7021 ttaagtacta ccaaacagtt aatatctcat taatatacag gcaataagcc agttgttaat
     7081 agtaagaatt agtacttgaa ctaaattgtt acctatttca tatgttgttg caattttgca
     7141 ataatcattt aagagatata gcctttaaaa tatatttgta gaattttgga cttatgtaaa
     7201 cagagcatct ttaggtgcca aaaactcatt ttgttatctt gtcatattag tgtcaaaaac
     7261 tatatactgt ataatatcca cccccccccc ccccaaaaaa aaaaataaat aaaaaaattc
     7321 agatatgtgt gtgtgtgtgt gtgtgtgtgt atattaggga tgcaacgatt atacattttg
     7381 gttgtacgat tatagtcatg gaaataatca cggtttcacg gttatcacaa ttattatgca
     7441 tttattaatt tcaagcccca ccagtttaga aaataagata aaaactcctt ttgctttaca
     7501 gtgttattta ttgctgctca gtagctaatt aataaagcac aaaataatat aaaaaacgaa
     7561 caatagtaaa tttctctctt tgtgcttaaa aataagtgaa tgtttttgac atttaaacaa
     7621 agaattttac actgtaaata acgcgttggg gtcgccacag cggaatgaac caccaactta
     7681 tccagcatat gttttacaca gcgaataccc ttccagtcgc aacccatcac tggaaaaatt
     7741 cccatacaca ctcattcaca cacacactcg tacactacaa tcaattttac catccctaat
     7801 ttacctgtac catatgtctt tggacttgtg gggtaaaccc atgtgaacaa caggagaaca
     7861 ttcaaactcc acacagaaac gccaactgac ccagccgagg ctcgaaccag caaccttctt
     7921 gctgtgaggc gaaggtgcta cccactacgg cactgcgtcg cccgcaatgt gtatatctta
     7981 gcaaaacaaa gcatcgcaat gtcgtatttc tccaatatcg ttcagcccta acctaaggtc
     8041 tgcactacac tacataatct aagtacagaa taagaggaag tccacaagtc aagagtgtgc
     8101 cgtttaagac aggaacttac atgcttatca actatccaga acactgcatg tactgtatgg
     8161 ccaactatta agaacacagt aacaagtctc tatcggcttt tcagatagca ctgaggcaga
     8221 gtgatttgca tgggaaagca ccactcacca tggttttcag atatcttctt tccgtgtgca
     8281 tctgttcatt aatgcgtatc atggctttga gtttgatgtc atgagttctt ttgtttgccg
     8341 ttgatgacct tgttggcatt cacttgcagc agtcattttg tttacagtca catgatttgc
     8401 attaaacaaa acacaattac aattctgtcg aacaagattt gcattcttgt ctgcggggat
     8461 gttgttgaat cttaaccctg tgtgtgtgtg tgtgtctttc agagctgtgg agtaattgtg
     8521 gggtgcaatc gaagggcgaa ttctgcagtc gacggtaccg cggtggagct ccagcttttg
     8581 ttccctttag tgagggttaa ttcaagtttg tacaaaaaag caggctccgc ggccgccccc
     8641 ttcacccaag ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcaccatg
     8701 aggtttcatc tgaagtttta cctgcattac ctggttctgc tgggttccag ctgtgtaatt
     8761 tcgacctatg gcccaaacca gagggcccag aagactggcg atatcctgct tggaggactt
     8821 ttcccaatgc attttggggt ggcctccaaa gaccaggatc ttgcagcacg gccagagtca
     8881 acagagtgtg tcagatataa cttccgtgga ttccgctggc tgcagtctat gatcttcgct
     8941 atagaggaga tcaataacag ctccactctt cttccaaaca ttacgcttgg ctaccgtata
     9001 tttgatacgt gcaacactgt ctcgaaagcg ttggaggcgt cactgagttt tgtcgcgcag
     9061 aataagattg attcactgaa tctggacgag ttctgtaact gcacagggaa catcccatca
     9121 accattgcag tggtcggagc ttctggatct gcagtgtcta cagccgttgc agacctgctg
     9181 ggtcttttct acatccctca gattagctat gcttcatcca gccgcctttt gagcaacaaa
     9241 aaccagtaca aatccttcat gaggacaatc cctacagacg agtaccaggc gattgccatg
     9301 gctgccatca tcgagcactt ccagtggaac tgggtgattg ctatcgcctc tgacgacgaa
     9361 tacggccggc caggcatcga aaagtttgag aatgaaatgt ttcaccggga catctgcatt
     9421 gacctcaacg ttttaatttc acagtacgtt gatgaagctg agattcgtcg cctggcagat
     9481 cgcatccaga actcatccgc caaggtaatt gttgtgtttg ctagcggacc agatatcgag
     9541 ccgttagtta aagagatggt gagacgaaac atcacagacc gcgtctggtt ggcgagcgaa
     9601 gcatgggcca gttccagtct agtcgccaaa ccagagtatc ttgacgtcat gggaggaacc
     9661 attggctttg ctctgagggc tggacatata cccggcttta aagacttctt acagcaggtc
     9721 catccaaaga agtcaagcca taacgaattt gtgcgagaat tttgggagga gacgttcaac
     9781 tgttatcttg aagacagccc aaggaatgct gatagtgaga atggcagtac gagtttcagg
     9841 ccgctgtgta ctggggaaga ggacattgcc agtgttgaga cgccatattt ggactacacg
     9901 cacctcagga tttcctataa cgtgtatgtg gctgtttatg ccatagcaca ggcacttcag
     9961 gacatactta cctgcactcc agggaaagga ttgttttcta atggctcttg tgcagatatt
    10021 aggaaagttg aagcatggca ggttctgaaa caactaagac acctcaactt catagacagc
    10081 atgggggaga gagtgcgctt tgacaacggc agtgagcttt cagccaatta taccatcata
    10141 aactggcatc gatcacccga ggatgggtct gtcgtattca aggaggttgg ctattacagt
    10201 atacataaca agaacgtggc caagctttcc attgacagga gcaaaatcct gtggaacgga
    10261 cgtctaactg aggtaccatt ttccaactgt agtgtggaat gtgaacctgg aacaaggaaa
    10321 ggaattattg atggcgaacc cacatgttgc tttgagtgta cagaatgctc ggatggggag
    10381 tacagtgatc ataaagatgc cagtttttgc gttaaatgcc caaacaactc ctggtccaac
    10441 ggcaatcaca cttcttgctt tctgaaggaa atcgagtttc tgtcctggtc cgaaccgttc
    10501 gggattgcgc tggccttatt tgcagtcctc ggggttctcc taacagcttt tgtgttgggt
    10561 gtttttgtgc aattccgtga tactccaatc gtgaaggcat caaaccgaga gctgtcgttt
    10621 cttttgcttt tctcgcttat atgctgtttc tccagctctc tcatattcat aggcgaacca
    10681 caggattgga cgtgccgggt acgccaacca gcattcggca tcagctttgt gttatgcatc
    10741 tcatgcattc tagtcaaaac caatcgtgtc ctgctagtgt tcgaagctaa aatccctaca
    10801 agtctccacc gtaagtggtg gggattaaac ctgcagttct tgctagtctt cctgttcacg
    10861 tttgtgcagg tgatgatctg cgtggtttgg ttgtacaacg ctccgccggg aagttacaag
    10921 aactacgaca tcgatgaaat catcttcatc acctgcaacg agggctccat gatggctctg
    10981 gggtttctga ttggctacac gtgcttgttg gccgccatct gttttttctt cgcgttcaag
    11041 tctcggaagc tgccggagaa tttcacggag gccaaattca taacgttcag catgctgatt
    11101 tttttcatcg tctggatctc ctttattccg gcgtacttca gcacttacgg caaattcgtc
    11161 tcggctgttg aggtcatagc aatcctggct tccagcttca gtttgcttgc ctgtatcttt
    11221 ttcaacaagg tgtatatcat tcttctgaaa ccgtcgagga atacgatcga ggaggtgcgc
    11281 tgtagcactg cggctcacgc ctttaaagct gcagcaaaag cgactttacg acacagttca
    11341 ggctttagaa agagatccag cagtgttggc ggctcctccg catcttcgcc ctcctcatcc
    11401 atcagcatga agaccaacgg gaatgaaatg gagtcaccgt ctacgcggag acacggctca
    11461 aaaccgaggg tgagctttgg aagtgggacg gtcagtctct ccttgagctt cgaggaggct
    11521 agaaactcga tgaaaggagg tggaggtgct gtgagcaagg gcgaggagct gttcaccggg
    11581 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
    11641 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
    11701 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
    11761 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
    11821 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
    11881 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
    11941 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
    12001 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
    12061 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
    12121 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
    12181 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
    12241 ctcggcatgg acgagctgta caagtaacct ctcgagcctc tagaactata gtgagtcgta
    12301 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
    12361 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
    12421 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
    12481 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta
    12541 ccaagggtgg gcgcgccgac ccagctttct tgtacaaagt ggggcgcgcc tacgtaacta
    12601 gtctcgagca actttattat acatagttga taattcactg gccgtcgttt tacggtacca
    12661 tcgatgatga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
    12721 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
    12781 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
    12841 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
    12901 attatgatcc tctagatcag atctgcgaag atacggccac gggtgctctt gatcctgtgg
    12961 ctgattttgg actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt
    13021 ccactgattg actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta
    13081 atagatgtga catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat
    13141 aatgtgaaaa cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc
    13201 gcaattcaat tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata
    13261 agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa
    13321 atgtaattaa gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc
    13381 cccaaaaata atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct
    13441 ggttcttgac cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc
    13501 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
    13561 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    13621 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    13681 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    13741 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    13801 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    13861 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    13921 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    13981 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    14041 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    14101 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    14161 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    14221 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    14281 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    14341 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    14401 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    14461 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    14521 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    14581 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    14641 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    14701 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    14761 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    14821 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    14881 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    14941 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    15001 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    15061 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    15121 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    15181 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    15241 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    15301 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    15361 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    15421 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    15481 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    15541 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    15601 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    15661 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tg
//
