LOCUS       pB5_R8_3i_A_GKS_        6078 bp ds-DNA     circular     07-SEP-2022
DEFINITION  SOD2 MTS_3xHA_left m.11922  TALE_2aa_DddE(41-148)_1xUGI_SOD2 3'UTR
            .
VERSION     .
KEYWORDS    TALEN-CCR5L18-NtermN1.
SOURCE      synthetic DNA construct
  ORGANISM  human, from Guilinger paper  .
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK
            and Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    904..1050
                     /locus_tag="MTS"
                     /label="MTS"
                     /ApEinfo_label="MTS"
                     /ApEinfo_fwdcolor="#5fd1d3"
                     /ApEinfo_revcolor="#5fd1d3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#5ac3fa"
                     /ApEinfo_revcolor="#5ac3fa"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1075..1128
                     /locus_tag="NES"
                     /label="NES"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#67d841"
                     /ApEinfo_revcolor="#67d841"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /locus_tag="CMV promoter"
                     /label="CMV promoter"
                     /ApEinfo_label="CMV promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1135..1167
                     /locus_tag="NES2 (MAPKK NES)"
                     /label="NES2 (MAPKK NES)"
                     /ApEinfo_label="NES2 (MAPKK NES)"
                     /ApEinfo_fwdcolor="#dff20e"
                     /ApEinfo_revcolor="#dff20e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           2062..2263
                     /created_by="bmok"
                     /modified_by="bmok"
                     /locus_tag="SOD2-3'UTR"
                     /label="SOD2-3'UTR"
                     /ApEinfo_label="SOD2-3'UTR"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1051..1074
                     /locus_tag="FLAG tag"
                     /label="FLAG tag"
                     /ApEinfo_label="FLAG tag"
                     /ApEinfo_fwdcolor="#388384"
                     /ApEinfo_revcolor="#388384"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1807..2055
                     /locus_tag="UGI"
                     /label="UGI"
                     /ApEinfo_label="UGI"
                     /ApEinfo_fwdcolor="#377d21"
                     /ApEinfo_revcolor="#377d21"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2309..2737
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /locus_tag="f1 ori"
                     /label="f1 ori"
                     /ApEinfo_label="f1 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2751..3081
                     /note="SV40 enhancer and early promoter"
                     /locus_tag="SV40 promoter"
                     /label="SV40 promoter"
                     /ApEinfo_label="SV40 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2932..3067
                     /note="SV40 origin of replication"
                     /locus_tag="SV40 ori"
                     /label="SV40 ori"
                     /ApEinfo_label="SV40 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        3129..3176
                     /note="synthetic bacterial promoter"
                     /locus_tag="EM7 promoter"
                     /label="EM7 promoter"
                     /ApEinfo_label="EM7 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3195..3593
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
                     GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
                     GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
                     /locus_tag="BSD"
                     /label="BSD"
                     /ApEinfo_label="BSD"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    3751..3872
                     /note="SV40 polyadenylation signal"
                     /locus_tag="SV40 poly(A) signal"
                     /label="SV40 poly(A) signal"
                     /ApEinfo_label="SV40 poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3788..3807)
                     /note="SV40 polyA, reverse primer"
                     /locus_tag="SV40pA-R"
                     /label="SV40pA-R"
                     /ApEinfo_label="SV40pA-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    3945..3961
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /locus_tag="lac operator"
                     /label="lac operator"
                     /ApEinfo_label="lac operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(3969..3999)
                     /note="promoter for the E. coli lac operon"
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    4014..4035
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /locus_tag="CAP binding site"
                     /label="CAP binding site"
                     /ApEinfo_label="CAP binding site"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4323..4911)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(5082..5942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1471..1794
                     /created_by="bmok"
                     /modified_by="bmok"
                     /locus_tag="DddAN"
                     /label="DddAN"
                     /ApEinfo_label="DddAN"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1741..1743
                     /locus_tag="T1380I"
                     /label="T1380I"
                     /ApEinfo_label="T1380I"
                     /ApEinfo_fwdcolor="#dff20e"
                     /ApEinfo_revcolor="#dff20e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(5943..6047)
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1174..1431
                     /locus_tag="ZF R8-3i-ATP8"
                     /label="ZF R8-3i-ATP8"
                     /ApEinfo_label="ZF R8-3i-ATP8"
                     /ApEinfo_fwdcolor="#51ab32"
                     /ApEinfo_revcolor="#51ab32"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1789..1791
                     /locus_tag="E1396K"
                     /label="E1396K"
                     /ApEinfo_label="E1396K"
                     /ApEinfo_fwdcolor="#dff20e"
                     /ApEinfo_revcolor="#dff20e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1432..1470
                     /locus_tag="Linker"
                     /label="Linker"
                     /ApEinfo_label="Linker"
                     /ApEinfo_fwdcolor="#388384"
                     /ApEinfo_revcolor="#388384"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accATGTTGG GGTTTGTGGG TCGGGTGGCC GCTGCTCCGG CCTCCGGGGC CTTGCGGAGA
      961 CTCACCCCTT CAGCGTCGCT GCCCCCAGCT CAGCTCTTAC TGCGGGCCGC TCCGACGGCG
     1021 GTCCATCCTG TCAGGGACTA TGCGGCGCAA gattacaagg acgacgatga caagGTGGAT
     1081 GAAATGACCA AAAAGTTCGG CACGCTCACC ATTCACGACA CCGAAAAGgg atcaCTTCAG
     1141 AAAAAGCTGG AGGAACTGGA GTTGGACgcc gctATGGCTG AGAGGCCCTA TGCTTGCCCT
     1201 GAGTGCGGTA AAAGCTTTTC Tacctccggg tccctgagcc ggCACATCCG CACCCACACC
     1261 GGCGAGAAGC CTTACGCATG TCCAGAGTGT GGGAAGTCAT TCTCTcaatc cggttccctt
     1321 acccgcCATA TCAGAACCCA CACTGGCGAA AAGCCGTACG CTTGCCCAGA ATGCGGTAAG
     1381 TCCTTCTCTc ggagcgacgc actctcacaa CATACCAAGA TACACCTGCG GggatccGGC
     1441 GGAGGAGGTT CTggcggttc tggaggaagt GGCAGCTACG CCCTGGGTCC GTATCAGATT
     1501 AGCGCCCCGC AGCTGCCAGC TTACAATGGT CAGACCGTGG GTACCTTCTA CTATGTGAAC
     1561 GACGCGGGCG GTCTGGAGAG CAAGGTGTTT AGCAGCGGCG GTCCAACCCC GTACCCAAAC
     1621 TATGCCAATG CCGGTCATGT GGAGGGTCAG AGCGCCCTGT TCATGCGTGA TAACGGCATC
     1681 AGCGAGGGTC TGGTGTTCCA CAACAACCCG GAAGGCACCT GCGGTTTTTG CGTGAACATG
     1741 AtCGAGACCC TGCTGCCGGA AAACGCGAAA ATGACCGTGG TGCCGCCGaA AGGTtctggc
     1801 ggctcaACTA ATCTGAGCGA CATCATTGAG AAGGAGACTG GGAAACAGCT GGTCATTCAG
     1861 GAGTCCATCC TGATGCTGCC TGAGGAGGTG GAGGAAGTGA TCGGCAACAA GCCAGAGTCT
     1921 GACATCCTGG TGCACACCGC CTACGACGAG TCCACAGATG AGAATGTGAT GCTGCTGACC
     1981 TCTGACGCCC CCGAGTATAA GCCTTGGGCC CTGGTCATCC AGGATTCTAA CGGCGAGAAT
     2041 AAGATCAAGA TGCTGtgata aaccacgatc gttatgctga tcatacccta atgatcccag
     2101 caagataatg tcctgtcttc taagatgtgc atcaagcctg gtacatactg aaaaccctat
     2161 aaggtcctgg ataatttttg tttgattatt cattgaagaa acatttattt tccaattgtg
     2221 tgaagttttt gactgttaat aaaagaatct gtcaaccatc aaacttctga ggcggaaaga
     2281 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     2341 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     2401 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     2461 cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     2521 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     2581 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2641 cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta
     2701 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     2761 tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc
     2821 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2881 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     2941 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3001 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     3061 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     3121 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     3181 tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     3241 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     3301 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     3361 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     3421 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgtcgacagg
     3481 tgcttctcga tctgcatcct gggatcaaag cgatagtgaa ggacagtgat ggacagccga
     3541 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacttc
     3601 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     3661 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     3721 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     3781 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3841 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     3901 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     3961 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4021 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4081 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4141 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4201 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4261 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4321 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4501 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4741 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     4801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4921 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4981 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5041 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5101 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5161 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5221 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5281 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5341 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5401 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5461 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5521 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5581 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5641 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5701 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5761 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5821 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5881 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5941 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6001 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6061 aaagtgccac ctgacgtc    
//
