LOCUS       eSpCas9_(1.1)_NoF_G1_3'UTR_G3_ATP1A1.xdna    8860 bp      DNA     circular   08/09/2022
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : eSpCas9(1.1)_No_FLAG.xdna [8427  nt] : (KpnI-B
            lunt(trimmed)[434] / XbaI[428]) to SP_gRNA_pUC19_N_ATP1A1_R1
            .xdna [427  nt] : (NheI[7] / HindIII-Blunt(filled)[434])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     promoter        1..249
                     /label=Human U6 Promoter
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=dnas_title="Human U6 Promoter" - vntifkey="29" - label=Human U6 Promoter - 1..249 \ 
     misc_feature    250..269
                     /label=G1 3'UTR SLBP
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    270..345
                     /label=chimeric guide RNA scaffold 1
                     /SerialCloner_Color=&h80FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="chimeric guide RNA scaffold" - 268..343 \ 
     misc_feature    346..351
                     /label=U6 terminator
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="U6 terminator" - 344..349 \ 
     promoter        491..753
                     /label=U6 promoter
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    754..773
                     /label=G3  ATP1A1
                     /SerialCloner_Color=&hFF0080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    774..849
                     /label=chimeric guide RNA scaffold 2
                     /SerialCloner_Color=&h80FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="chimeric guide RNA scaffold" - 268..343 \ 
     misc_feature    863..1661
                     /label=CBh Promoter
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="CBh" - 440..1238 \ 
     misc_feature    5883..6114
                     /label=bGH polyA
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="bGH polyA" - 5529..5760 \ 
     misc_feature    1668..1677
                     /label=Kozak
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ATMX" \ 
     misc_feature    1680..1700
                     /label=SV40 NLS
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="PKKKRKV" \ 
     misc_feature    1725..5825
                     /label=eSpCas9 (1.1) ORF
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4263..4265
                     /label=K848A
                     /SerialCloner_Color=&hFF0080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="A" \ 
     misc_feature    4728..4730
                     /label=K1003A
                     /SerialCloner_Color=&hFF0080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="A" \ 
     misc_feature    4899..4901
                     /label=R1060A 
                     /SerialCloner_Color=&hFF0080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="A" \ 
     misc_feature    5826..5873
                     /label=Nucleoplasmin NLS
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6123..6263
                     /label=R-ITR
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=dnas_title="R-ITR" - vntifkey="21" - label=R-ITR - 5769..5909 \ 
     rep_origin      6355..6661
                     /label=f1 Origin
                     /SerialCloner_Color=&h666666
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=dnas_title="f1 Origin" - vntifkey="33" - label=f1 Origin - 6001..6307 \ 
     misc_feature    complement(7115..7179)
                     /label=bla\promoter
                     /SerialCloner_Color=&h408080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=blapromoter - 2487..2551 \ 
     misc_feature    7180..8037
                     /label=AmpR
                     /SerialCloner_Color=&h666666
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=dnas_title="AmpR" - vntifkey="21" - label=AmpR - 6826..7683 \ 
     misc_feature    complement(8212..8799)
                     /label=Ori
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="21" - label=Ori - 867..1454 \ 
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga 
      241 cgaaacaccG TGAGCTAACT GCCCCCTGGg ttttagagct agaaatagca agttaaaata 
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag 
      361 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag 
      421 acaaatggct ctagCTGTAC AAAAAAGCAG GCTTTAAAGG AACCAATTCA GTCGACTGGA 
      481 TCCGGTACCA AGGTCGGGCA GGAAGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT 
      541 ATACGATACA AGGCTGTTAG AGAGATAATT AGAATTAATT TGACTGTAAA CACAAAGATA 
      601 TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA 
      661 TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG 
      721 GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGAGTTCT GTAATTCAGC ATAGTTTTAG 
      781 AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG 
      841 AGTCGGTGCT TTTTTTAagc tccgttacat aacttacggt aaatggcccg cctggctgac 
      901 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac 
      961 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
     1021 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc 
     1081 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
     1141 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc 
     1201 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg 
     1261 gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc 
     1321 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga 
     1381 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga 
     1441 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga 
     1501 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat 
     1561 tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat 
     1621 tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc accATGGCCC 
     1681 CAAAGAAGAA GCGGAAGGTC GGTATCCACG GAGTCCCAGC AGCCGACAAG AAGTACAGCA 
     1741 TCGGCCTGGA CATCGGCACC AACTCTGTGG GCTGGGCCGT GATCACCGAC GAGTACAAGG 
     1801 TGCCCAGCAA GAAATTCAAG GTGCTGGGCA ACACCGACCG GCACAGCATC AAGAAGAACC 
     1861 TGATCGGAGC CCTGCTGTTC GACAGCGGCG AAACAGCCGA GGCCACCCGG CTGAAGAGAA 
     1921 CCGCCAGAAG AAGATACACC AGACGGAAGA ACCGGATCTG CTATCTGCAA GAGATCTTCA 
     1981 GCAACGAGAT GGCCAAGGTG GACGACAGCT TCTTCCACAG ACTGGAAGAG TCCTTCCTGG 
     2041 TGGAAGAGGA TAAGAAGCAC GAGCGGCACC CCATCTTCGG CAACATCGTG GACGAGGTGG 
     2101 CCTACCACGA GAAGTACCCC ACCATCTACC ACCTGAGAAA GAAACTGGTG GACAGCACCG 
     2161 ACAAGGCCGA CCTGCGGCTG ATCTATCTGG CCCTGGCCCA CATGATCAAG TTCCGGGGCC 
     2221 ACTTCCTGAT CGAGGGCGAC CTGAACCCCG ACAACAGCGA CGTGGACAAG CTGTTCATCC 
     2281 AGCTGGTGCA GACCTACAAC CAGCTGTTCG AGGAAAACCC CATCAACGCC AGCGGCGTGG 
     2341 ACGCCAAGGC CATCCTGTCT GCCAGACTGA GCAAGAGCAG ACGGCTGGAA AATCTGATCG 
     2401 CCCAGCTGCC CGGCGAGAAG AAGAATGGCC TGTTCGGAAA CCTGATTGCC CTGAGCCTGG 
     2461 GCCTGACCCC CAACTTCAAG AGCAACTTCG ACCTGGCCGA GGATGCCAAA CTGCAGCTGA 
     2521 GCAAGGACAC CTACGACGAC GACCTGGACA ACCTGCTGGC CCAGATCGGC GACCAGTACG 
     2581 CCGACCTGTT TCTGGCCGCC AAGAACCTGT CCGACGCCAT CCTGCTGAGC GACATCCTGA 
     2641 GAGTGAACAC CGAGATCACC AAGGCCCCCC TGAGCGCCTC TATGATCAAG AGATACGACG 
     2701 AGCACCACCA GGACCTGACC CTGCTGAAAG CTCTCGTGCG GCAGCAGCTG CCTGAGAAGT 
     2761 ACAAAGAGAT TTTCTTCGAC CAGAGCAAGA ACGGCTACGC CGGCTACATT GACGGCGGAG 
     2821 CCAGCCAGGA AGAGTTCTAC AAGTTCATCA AGCCCATCCT GGAAAAGATG GACGGCACCG 
     2881 AGGAACTGCT CGTGAAGCTG AACAGAGAGG ACCTGCTGCG GAAGCAGCGG ACCTTCGACA 
     2941 ACGGCAGCAT CCCCCACCAG ATCCACCTGG GAGAGCTGCA CGCCATTCTG CGGCGGCAGG 
     3001 AAGATTTTTA CCCATTCCTG AAGGACAACC GGGAAAAGAT CGAGAAGATC CTGACCTTCC 
     3061 GCATCCCCTA CTACGTGGGC CCTCTGGCCA GGGGAAACAG CAGATTCGCC TGGATGACCA 
     3121 GAAAGAGCGA GGAAACCATC ACCCCCTGGA ACTTCGAGGA AGTGGTGGAC AAGGGCGCTT 
     3181 CCGCCCAGAG CTTCATCGAG CGGATGACCA ACTTCGATAA GAACCTGCCC AACGAGAAGG 
     3241 TGCTGCCCAA GCACAGCCTG CTGTACGAGT ACTTCACCGT GTATAACGAG CTGACCAAAG 
     3301 TGAAATACGT GACCGAGGGA ATGAGAAAGC CCGCCTTCCT GAGCGGCGAG CAGAAAAAGG 
     3361 CCATCGTGGA CCTGCTGTTC AAGACCAACC GGAAAGTGAC CGTGAAGCAG CTGAAAGAGG 
     3421 ACTACTTCAA GAAAATCGAG TGCTTCGACT CCGTGGAAAT CTCCGGCGTG GAAGATCGGT 
     3481 TCAACGCCTC CCTGGGCACA TACCACGATC TGCTGAAAAT TATCAAGGAC AAGGACTTCC 
     3541 TGGACAATGA GGAAAACGAG GACATTCTGG AAGATATCGT GCTGACCCTG ACACTGTTTG 
     3601 AGGACAGAGA GATGATCGAG GAACGGCTGA AAACCTATGC CCACCTGTTC GACGACAAAG 
     3661 TGATGAAGCA GCTGAAGCGG CGGAGATACA CCGGCTGGGG CAGGCTGAGC CGGAAGCTGA 
     3721 TCAACGGCAT CCGGGACAAG CAGTCCGGCA AGACAATCCT GGATTTCCTG AAGTCCGACG 
     3781 GCTTCGCCAA CAGAAACTTC ATGCAGCTGA TCCACGACGA CAGCCTGACC TTTAAAGAGG 
     3841 ACATCCAGAA AGCCCAGGTG TCCGGCCAGG GCGATAGCCT GCACGAGCAC ATTGCCAATC 
     3901 TGGCCGGCAG CCCCGCCATT AAGAAGGGCA TCCTGCAGAC AGTGAAGGTG GTGGACGAGC 
     3961 TCGTGAAAGT GATGGGCCGG CACAAGCCCG AGAACATCGT GATCGAAATG GCCAGAGAGA 
     4021 ACCAGACCAC CCAGAAGGGA CAGAAGAACA GCCGCGAGAG AATGAAGCGG ATCGAAGAGG 
     4081 GCATCAAAGA GCTGGGCAGC CAGATCCTGA AAGAACACCC CGTGGAAAAC ACCCAGCTGC 
     4141 AGAACGAGAA GCTGTACCTG TACTACCTGC AGAATGGGCG GGATATGTAC GTGGACCAGG 
     4201 AACTGGACAT CAACCGGCTG TCCGACTACG ATGTGGACCA TATCGTGCCT CAGAGCTTTC 
     4261 TGGCCGACGA CTCCATCGAC AACAAGGTGC TGACCAGAAG CGACAAGAAC CGGGGCAAGA 
     4321 GCGACAACGT GCCCTCCGAA GAGGTCGTGA AGAAGATGAA GAACTACTGG CGGCAGCTGC 
     4381 TGAACGCCAA GCTGATTACC CAGAGAAAGT TCGACAATCT GACCAAGGCC GAGAGAGGCG 
     4441 GCCTGAGCGA ACTGGATAAG GCCGGCTTCA TCAAGAGACA GCTGGTGGAA ACCCGGCAGA 
     4501 TCACAAAGCA CGTGGCACAG ATCCTGGACT CCCGGATGAA CACTAAGTAC GACGAGAATG 
     4561 ACAAGCTGAT CCGGGAAGTG AAAGTGATCA CCCTGAAGTC CAAGCTGGTG TCCGATTTCC 
     4621 GGAAGGATTT CCAGTTTTAC AAAGTGCGCG AGATCAACAA CTACCACCAC GCCCACGACG 
     4681 CCTACCTGAA CGCCGTCGTG GGAACCGCCC TGATCAAAAA GTACCCTGCG CTGGAAAGCG 
     4741 AGTTCGTGTA CGGCGACTAC AAGGTGTACG ACGTGCGGAA GATGATCGCC AAGAGCGAGC 
     4801 AGGAAATCGG CAAGGCTACC GCCAAGTACT TCTTCTACAG CAACATCATG AACTTTTTCA 
     4861 AGACCGAGAT TACCCTGGCC AACGGCGAGA TCCGGAAGGC GCCTCTGATC GAGACAAACG 
     4921 GCGAAACCGG GGAGATCGTG TGGGATAAGG GCCGGGATTT TGCCACCGTG CGGAAAGTGC 
     4981 TGAGCATGCC CCAAGTGAAT ATCGTGAAAA AGACCGAGGT GCAGACAGGC GGCTTCAGCA 
     5041 AAGAGTCTAT CCTGCCCAAG AGGAACAGCG ATAAGCTGAT CGCCAGAAAG AAGGACTGGG 
     5101 ACCCTAAGAA GTACGGCGGC TTCGACAGCC CCACCGTGGC CTATTCTGTG CTGGTGGTGG 
     5161 CCAAAGTGGA AAAGGGCAAG TCCAAGAAAC TGAAGAGTGT GAAAGAGCTG CTGGGGATCA 
     5221 CCATCATGGA AAGAAGCAGC TTCGAGAAGA ATCCCATCGA CTTTCTGGAA GCCAAGGGCT 
     5281 ACAAAGAAGT GAAAAAGGAC CTGATCATCA AGCTGCCTAA GTACTCCCTG TTCGAGCTGG 
     5341 AAAACGGCCG GAAGAGAATG CTGGCCTCTG CCGGCGAACT GCAGAAGGGA AACGAACTGG 
     5401 CCCTGCCCTC CAAATATGTG AACTTCCTGT ACCTGGCCAG CCACTATGAG AAGCTGAAGG 
     5461 GCTCCCCCGA GGATAATGAG CAGAAACAGC TGTTTGTGGA ACAGCACAAG CACTACCTGG 
     5521 ACGAGATCAT CGAGCAGATC AGCGAGTTCT CCAAGAGAGT GATCCTGGCC GACGCTAATC 
     5581 TGGACAAAGT GCTGTCCGCC TACAACAAGC ACCGGGATAA GCCCATCAGA GAGCAGGCCG 
     5641 AGAATATCAT CCACCTGTTT ACCCTGACCA ATCTGGGAGC CCCTGCCGCC TTCAAGTACT 
     5701 TTGACACCAC CATCGACCGG AAGAGGTACA CCAGCACCAA AGAGGTGCTG GACGCCACCC 
     5761 TGATCCACCA GAGCATCACC GGCCTGTACG AGACACGGAT CGACCTGTCT CAGCTGGGAG 
     5821 GCGACAAAAG GCCGGCGGCC ACGAAAAAGG CCGGCCAGGC AAAAAAGAAA AAGTAAgaat 
     5881 tcctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 
     5941 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 
     6001 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 
     6061 ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg gggagcggcc 
     6121 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga 
     6181 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga 
     6241 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg 
     6301 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta 
     6361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 
     6421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 
     6481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 
     6541 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt 
     6601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 
     6661 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc 
     6721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 
     6781 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc 
     6841 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 
     6901 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 
     6961 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat 
     7021 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 
     7081 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 
     7141 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 
     7201 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 
     7261 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 
     7321 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 
     7381 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 
     7441 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 
     7501 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 
     7561 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 
     7621 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 
     7681 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 
     7741 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 
     7801 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 
     7861 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg 
     7921 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 
     7981 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 
     8041 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 
     8101 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 
     8161 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 
     8221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 
     8281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 
     8341 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 
     8401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 
     8461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 
     8521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 
     8581 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 
     8641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 
     8701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 
     8761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 
     8821 ttacggttcc tggccttttg ctggcctttt gctcacatgt 
//