LOCUS       pHAGE-NLS-dMisCa       12769 bp DNA     circular SYN 18-JUL-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12769)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12769
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        2242..2453
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /note="color: #------"
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    3471
                     /label=Feature 17
                     /note="color: #a6acb3"
     CDS             3472..7254
                     /codon_start=1
                     /label=MisCas13b
                     /note="color: #993366"
                     /translation="MFVRYSPESWPRHFHGLQQRLPTPIGQHTFLSDNFNTMDIRLSKN
                     RALLAAYLNMAQQNVYVTLLHISQLMGVEETKDLSNRMYDFQILQELKTGKKGAESEQM
                     VKSERVQKIILLLYRNFPFLKAFVEAKYKQQEKNNGGKNKGGGKNTQKNKKTPDNTSNE
                     EQLEFTESELGKLPIPPHEYRTILCDVFRLINDLANEYTAFSPEKRTGGAKESHEKCQK
                     KMLSYLYLCLDGGRRVVKERFKDTFQEKDFNFLTQGPRYSLQKDGDKKKAEERPNFYYR
                     LKDDQGLFSYVGILLFISLFLHKRYISEMLAQSGLFYTPRYWKGQPVNVELERRIISEI
                     FSIYRIRLPKERIDSTRPDYALGLDMLNELQKCPNELYETFSPKDQAEFRVKREYDESG
                     EGTEEGEVLLKRFEDRFPYFAIRYLDENRVFDDIRFQVSIGKYRYKFYNKLCIDVVSSN
                     RNDDCYRLRSLQKEINGFGRLNEVEYERILRYGSHIHVEKPEGDDTPYLTDHHTSYLFN
                     GNRIGLLFNQENGRHDLEEIRGNCLYLPHLTFASSSEGKDARRVPPIAWLSTYELPALI
                     FHHLLCRHYGLERTATESIIKECVGLYHRLFTDIRDGKLLPGEALGIQAEESTRSEISR
                     IKKGEPLPQNISEKIETFLSTHYCTEQVCLHIQDIPKKIIDYLIGKNIDQDMSGLFKQR
                     SRERINKMYESTKIRLKKFKTDLDLQGNGDNKFGKKRYTDIRPGQLADYLAKDLLFFQP
                     YDDEKKNKVTGLNYQILQAALAIYNHNLDELRRLFKNARLLVPENPKMEHPFLGSVLHK
                     SPRTTLQFYAYYLLEKISYLKKRLDDPNETFADLFRYKQRQKWQTRDSQWYRALAGRYM
                     AEMMEEGTMNFKPVELPRGLFERPIKRLLTNILTKRTDSDSQALLKIVESPRCNVSYLI
                     AQYFTLILHDDSQPFYHPNKRYKRTYQYFNILNNRKERNKLVQVYYTLDEMEAYFKDTE
                     SLKKRYKDYLTSSEAKKHPAPEQRLKKYLSEYRKNETTIRRYRVQDMLVFLMAKILLLE
                     KGGLEAGELEQYKLSHIQPTTGQNSGQNILSLQVPFNITVKLDDGTERSITQEVIKVKN
                     YGDFFRFLYDDRVASLLPYTQEDIPLERALLEKELEGYDRERIPIFKSVHCMEKEILRL
                     CPELNDPQSSQYKYTTDIGTEKIVKENFRAMLTELCSRGLLPENEIEMMVEIANSFSAN
                     RYACVNLKGKNIPMIAISIQRKFDGVVGRTIGKNKN"
     misc_feature    3988..4032
                     /label=Feature 18
                     /note="color: #a6acb3"
     primer_bind     4498..4517
                     /label=Primer 1
                     /note="color: black; sequence: atctatcgcatcaggctgcc"
     primer_bind     complement(4517..4536)
                     /label=dMis-H1-Pgu-R
                     /note="color: black; sequence: 
                     atccagtcgtccatccgcagggagtcgattctctccttgg"
     primer_bind     4546..4565
                     /label=Primer 2
                     /note="color: black; sequence: gattacgccctcggcctgga"
     misc_feature    5092..5133
                     /label=Feature 19
                     /note="color: #a6acb3"
     misc_feature    5332..5451
                     /label=Feature 20
                     /note="color: #a6acb3"
     primer_bind     complement(5339..5350)
                     /label=Primer 2
                     /note="color: black; sequence: gattacgccctcggcctgga"
     primer_bind     complement(6695..6714)
                     /label=Primer 3
                     /note="color: black; sequence: ctgcaggctcaggatgttct"
     CDS             7264..7809
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /note="color: #cc99b2"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             7816..7863
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7882..7947
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7954..8521
                     /label=IRES
                     /note="This feature has 2 segments:
                        1: 7954 .. 8393 / #a6acb3
                        2: 8394 .. 8521 / #a6acb3"
     CDS             8541..9140
                     /codon_start=1
                     /label=PuroR
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9159..9747
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(9630..9641)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2"
                     /translation="IEGR"
     LTR             9822..10055
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        10260..10364
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             10365..11225
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10365 .. 10433 / #ccffcc / signal sequence
                        2: 10434 .. 11225 / #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11396..11984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    12395..12416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     promoter        12431..12461
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 12431 .. 12436 / #ffffff / -35
                        2: 12437 .. 12454 / #ffffff
                        3: 12455 .. 12461 / #ffffff / -10"
     protein_bind    12469..12485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     12493..12509
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag catgttcgtg
     3481 agatatagcc ctgagtcctg gcctaggcac ttccacggcc tgcagcagag actgcccacc
     3541 cccatcggcc agcacacctt cctgagtgat aactttaaca ccatggacat ccggctgagt
     3601 aagaatagag ccctgctggc agcttacctg aatatggccc agcagaacgt gtacgtgacc
     3661 ctgctgcaca tctcccagct gatgggagtg gaggagacca aagacctgtc taaccgcatg
     3721 tacgactttc agattctgca ggagcttaag accgggaaga agggcgccga gtctgagcag
     3781 atggtgaagt ctgagagagt gcagaagatc attctcctgc tgtatcgcaa cttccctttc
     3841 ctgaaggcct tcgtggaggc taagtacaag cagcaggaga agaacaatgg gggcaaaaac
     3901 aagggcggcg gaaaaaacac acagaagaat aagaaaaccc ctgacaatac aagcaacgag
     3961 gagcagctgg agttcactga gagtgagctg ggcaagctgc ccattccacc acatgagtac
     4021 agaaccatcc tgtgtgatgt gttcaggctg atcaatgacc tggccaatga gtacacagcc
     4081 ttcagccctg agaaacgcac tggcggcgct aaggagtccc acgaaaagtg ccagaagaag
     4141 atgctgagct acctgtacct gtgcctggac ggaggcagaa gggtggtgaa ggagcggttc
     4201 aaggacacct tccaagagaa ggactttaac tttctcaccc agggcccaag atacagcctc
     4261 cagaaagacg gagataagaa gaaggccgag gaaagaccta atttttacta cagactgaag
     4321 gatgaccagg gactgttctc ttacgtcggt atcctcctgt tcatcagcct gttcctgcac
     4381 aagaggtata tttccgagat gctggcccag tccgggctgt tttatacacc tcggtactgg
     4441 aagggccagc cagtgaacgt ggaactggag aggaggatca tcagtgagat cttttccatc
     4501 tatcgcatca ggctgcccaa ggagagaatc gactccacca gaccagatta cgccctcggc
     4561 ctggatatgc tgaatgagct gcagaagtgc cctaatgagc tgtatgagac cttctcacct
     4621 aaagaccagg ctgagtttag agtgaagcgg gagtatgacg agagcggcga aggcacagaa
     4681 gagggcgagg tgctgctgaa aagattcgag gacagattcc cttatttcgc cattcgctat
     4741 ctggacgaga atagagtgtt cgacgacatc agattccagg tgagcatcgg caagtatagg
     4801 tacaaattct acaataagct gtgcatcgac gtggtgagca gcaatcggaa cgatgactgc
     4861 tatcgcctga ggtccctgca gaaagagatt aatggcttcg gcagactgaa tgaagtggag
     4921 tacgagagaa tcctgagata cgggtctcac atccacgtgg agaagcctga gggcgacgac
     4981 accccctacc tgactgacca tcataccagc tacctgttta acggcaatag gatcggcctg
     5041 ctgttcaacc aggagaacgg caggcacgat ctggaggaga tcagaggaaa ctgcctgtat
     5101 ctgcctcacc tgacttttgc atcctcctcc gagggaaagg atgccaggcg cgtgcccccc
     5161 atcgcctggc tgtccaccta tgagctgcct gccctgatct tccaccacct gctgtgcaga
     5221 cactatggcc tggagagaac tgccaccgag agcatcatca aggaatgtgt gggcctgtac
     5281 caccggctgt ttactgatat ccgcgacggt aaactgctcc ccggagaggc cctgggcatc
     5341 caggccgagg agtccacaag gagcgagatt agtaggatca agaagggtga acccctgccc
     5401 cagaacatct ctgaaaagat tgaaaccttc ctgagcactc actactgcac cgaacaggtg
     5461 tgcctgcaca tccaggacat tcctaagaaa atcatcgatt acctgatcgg gaaaaacatc
     5521 gaccaggaca tgagcggact ctttaagcag agaagcagag agcggattaa taagatgtat
     5581 gagtccacta aaattcgcct caagaagttc aagaccgacc tggacctgca gggcaacggc
     5641 gacaataagt ttggaaagaa gaggtacact gacattaggc caggacagct ggccgactat
     5701 ctggccaagg acctgctgtt cttccagcca tatgacgacg agaagaagaa taaggtcact
     5761 ggactgaact atcagatcct gcaggccgca ctggccatct acaatcacaa cctggatgag
     5821 ctcagacgac tcttcaaaaa tgcccggctg ctcgtgcccg agaatcctaa gatggagcac
     5881 cctttcctgg gctctgtgct gcacaagtcc ccccggacca ctctgcagtt ctacgcctac
     5941 tacctgctcg agaagatctc ttacctgaaa aagagactcg acgatcctaa cgagacattc
     6001 gccgatctgt ttagatataa gcagaggcag aaatggcaga ctagagacag ccagtggtat
     6061 agggccttgg ctggcaggta catggctgag atgatggaag agggcaccat gaattttaaa
     6121 cccgtggagc tgccaagagg gctgtttgaa agacctatca agcggctgct gaccaatatc
     6181 ctgactaagc ggaccgacag cgatagccag gccctgctga agatcgtgga gagccccagg
     6241 tgcaacgtga gctacctgat tgctcagtat ttcactctga ttctgcacga cgattcccag
     6301 cccttttacc accctaacaa acgctacaaa aggacctacc agtatttcaa catcctgaac
     6361 aatagaaagg agcggaataa gctcgtgcag gtgtactaca ctctggatga gatggaggct
     6421 tactttaagg ataccgaatc tctgaagaag aggtataagg actacctgac tagctccgag
     6481 gctaagaagc acccagcccc cgagcagagg ctgaagaagt atctgtcaga gtatcgcaag
     6541 aatgagacca ccattagacg gtacagagtg caggacatgc tggtgtttct gatggccaaa
     6601 attctgctgc tggaaaaggg cggcctcgaa gccggcgagc tggagcagta caagctgtcc
     6661 cacattcagc ccaccaccgg ccagaacagc ggccagaaca tcctgagcct gcaggtgccc
     6721 tttaacatca ccgtgaagct ggacgatggc acagagagaa gcatcaccca ggaagtgatc
     6781 aaggtgaaaa attatggcga cttctttaga tttctctatg atgaccgggt ggcatccctg
     6841 ctgccttata ctcaggagga cattcccctg gagcgggccc tgctggagaa ggagctggag
     6901 ggatacgaca gggagagaat ccctattttc aagagcgtgc actgcatgga gaaggagatc
     6961 ctgaggctgt gccctgaact gaatgatccc cagtccagcc agtacaagta taccacagat
     7021 attggcactg agaagatcgt gaaggagaat ttcagggcca tgctgactga gctgtgtagc
     7081 cggggcctgc tccctgagaa cgagatcgag atgatggtgg agatcgccaa tagcttttcc
     7141 gccaatagat acgcctgcgt gaacctgaag ggcaagaata tcccaatgat cgccatctcc
     7201 atccagcgga agttcgacgg cgtggtgggc agaaccatcg gaaagaataa gaacagtcta
     7261 gaaatggaca aagactgcga aatgaagcgc accaccctgg atagccctct gggcaagctg
     7321 gaactgtctg ggtgcgaaca gggcctgcac cgtatcatct tcctgggcaa aggaacatct
     7381 gccgccgacg ccgtggaagt gcctgcccca gccgccgtgc tgggcggacc agagccactg
     7441 atgcaggcca ccgcctggct caacgcctac tttcaccagc ctgaggccat cgaggagttc
     7501 cctgtgccag ccctgcacca cccagtgttc cagcaggaga gctttacccg ccaggtgctg
     7561 tggaaactgc tgaaagtggt gaagttcgga gaggtcatca gctacagcca cctggccgcc
     7621 ctggccggca atcccgccgc caccgccgcc gtgaaaaccg ccctgagcgg aaatcccgtg
     7681 cccattctga tcccctgcca ccgggtggtg cagggcgacc tggacgtggg gggctacgag
     7741 ggcgggctcg ccgtgaaaga gtggctgctg gcccacgagg gccacagact gggcaagcct
     7801 gggctgggtc atatgaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag
     7861 aaacaacgcg tgcagctaag cgactacaaa gaccatgacg gtgattataa agatcatgac
     7921 atcgattaca aggatgacga tgacaagtaa ggatccctcc ccccccccta acgttactgg
     7981 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     8041 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     8101 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     8161 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     8221 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     8281 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     8341 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     8401 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     8461 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     8521 aggatccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     8581 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     8641 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     8701 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     8761 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     8821 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     8881 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     8941 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     9001 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     9061 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     9121 cccgcaagcc cggtgcctga cgcccatcga tagatcctaa tcaacctctg gattacaaaa
     9181 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     9241 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     9301 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     9361 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9421 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9481 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9541 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     9601 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9661 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9721 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     9781 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     9841 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9901 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9961 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10021 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
    10081 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
    10141 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
    10201 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    10261 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10321 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10381 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10441 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10501 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10561 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
    10621 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10681 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10741 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10801 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10861 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10921 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10981 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11041 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    11101 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    11161 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11221 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11281 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11341 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    11401 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    11461 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    11521 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    11581 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    11641 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11701 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11761 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11821 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11881 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11941 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12001 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    12061 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    12121 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    12181 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
    12241 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    12301 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
    12361 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12421 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12481 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
    12541 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    12601 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    12661 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    12721 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
