LOCUS       BB112_pRS426_csr       10751 bp DNA     circular SYN 09-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Dunlap-Loros
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          5237..5953
                     /mol_type="other DNA"
                     /db_xref="taxon:274625"
                     /organism="Expression vector pMF272"
     promoter        196..416
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             417..1220
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    1948..2940
                     /label=N crassa csr-1 5' homology
     misc_feature    2971..2993
                     /label=1 kb f primer
     misc_feature    3180..3198
                     /label=800 bp f primer
     misc_feature    3395..3414
                     /label=600 bp f primer
     misc_feature    3603..3624
                     /label=400 bp f primer
     misc_feature    3796..3814
                     /label=200 bp f primer
     misc_feature    3977..4001
                     /label=r primer
     mRNA            join(4001..4265,4332..4783,4855..5209)
                     /locus_tag="NCU04288"
                     /product="Poly(A)+ RNA export protein"
                     /label=NCU04288 mRNA
                     /db_xref="GI:758997004"
                     /db_xref="GeneID:3877232"
     CDS             join(4002..4265,4332..4783,4855..5209)
                     /codon_start=1
                     /locus_tag="NCU04288"
                     /product="Poly(A)+ RNA export protein"
                     /label=NCU04288
                     /db_xref="GI:85101044"
                     /db_xref="GeneID:3877232"
                     /protein_id="XP_961078.1"
                     /translation="MAGLFGSAAASASNTLGDLKQDVELGQPPEDSISDLAFNPNPADQ
                     KDFLAVASWDKKTRIYEILSNGQGQGQAMIEHDAPVFSCDFFKDGTKVISAGADKAAKV
                     LDLATGQSMQVAAHDMPIKCVRYFEANGTPMAVTGGWDKQIKYWDFRSANPAATVQAQE
                     RVYTMDVRDNLLVVGTADRYINVINLKDPGKFYKTMQSPLKWQTRVVSCFNDSQGFAIG
                     SIEGRCAIQYVEDKDSASNFSFKCHRDPAQGNTTAVHAVNDISFHPQHGTFSTAGSDGT
                     FHFWDKDAKHRLKGYPNVGGSITSTTFNKTGSIFAYAISYDWSKGYQGNSPTYPTKVML
                     HPVQQDECKPRPSVKKR"
     misc_feature    5210..5236
                     /label=linker
     CDS             complement(5236)
                     /codon_start=1
                     /label=ORF frame 3
                     /translation=""
     CDS             5237..5956
                     /codon_start=1
                     /gene="sgfp"
                     /product="green fluorescent protein S65T variant"
                     /label=sgfp
                     /note="enhanced version of GFP uses human codon bias"
                     /db_xref="GI:47524499"
                     /protein_id="AAT34982.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            5237..5953
                     /gene="sgfp"
                     /label=sgfp
     misc_feature    5982..7193
                     /label=N crassa csr-1 3' homology
     protein_bind    7212..7228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7236..7266)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(7590..8178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8349..9209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9210..9314)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9341..10683
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgct ggagaagctc attccacccg aaatgcccgt
     1981 gaacttgccc ttgtttgcgc gggccttctt gcgctctgct cggatacgct cgacatcgct
     2041 gaggagctcg gcgagttcct tggcgcggtt gcggacgttg ataccctggt ctttgccgtt
     2101 ctggtcgata aaatggaact ggcgcagcat cttgaggagg gtaagatgcg accgagcatc
     2161 gtccaccact cgttcggaac cgtgcttaat caggtactct agtagttgaa gggccttgta
     2221 gatctggcgc cattcttcgg cggacttttc tgtgaaacgg cggtaaatca tgggcatgat
     2281 ttcgttgagt gtttggctaa gaacaaggag ggaaacgcaa gttagccgcg ttcgtataca
     2341 agcgagcagt tctcgagaac gtactagttg aaggtgccat cggcgatctc ttgcatcaaa
     2401 gtcgacgatg caccccaagg ttcattgttg gtggcctcgc gcacctgggt tcgctgtcag
     2461 ctcccgttcc atggtctctt gtctgggatc aagtatatgg cgaagggcgc aagagaatgg
     2521 tgccagtgtg gcgcgtggat acgggcatac cttggactcc atctcggtat agttcatgac
     2581 agctggttgg agccattgtc agcaccctga acacgaaatc atgagggagt cttggttctg
     2641 gactaggtgg tcacctacca ttttgaacct ttcgtacgcc agccttgatg tcgtagagtg
     2701 tcaggttgga cacggtatcc ttgaggctat tgaaatccat tgcgtaaacg tgtctatcgc
     2761 cggagttgct gggaattgtt ctgatgggct aaggtaattc ttctctagat ccaatctgag
     2821 cctcgcggtg atgattgcca gacgggcggg atgctgtggc gacttggtgg tttttgttcg
     2881 ggctgatcgc tttcagggcg acgtttcacg gtcttgtcgg gatcagatgg acattgaggt
     2941 gcggaattat acgatttagg tgactgcagg ccatacagat ctccctagac gccgggccaa
     3001 tccgttcctt gcaccttctc tcacacatgc aggttttgga atgtggcttc atcgtgtcca
     3061 gaactgggcg cattggggcc aggctcagtg tcagtgtagg gatcgatgta catatggcag
     3121 ttgacagcga gagagccagg tgtcacaggc agtccgtcag tggccgcggt ggtggccacg
     3181 cactttctgc aggagccgtc tggggtgcgt gcagctggcg gccgcttccg ggcatacggc
     3241 ttgtgtaacg agggtgttga ccctcggctt ggcgcttgaa cttggcgctg cggtcccccc
     3301 acagttgacc acaatccccg gccaatcgaa cgacggcaac aagggcgcac tagccagtga
     3361 acgcctgggg atagtgggtg gtcaaagaga attgggcaaa cgagacgaca atggagggtg
     3421 aaccgggaat aataaacagc tggtgagacc acccatcaaa cgaatcaccc caccatggga
     3481 tgccctcaga tgccctccat gccaccataa ggaatgatct tacggcttgc cgaagttaac
     3541 aggcctgctt ttgcatatga acgagagcct ggtgttgcat tccacattgg ctatctacag
     3601 tacctaccca acctacacac cctgatgcga aagggacaga aggggagaag gatacataca
     3661 tatacctgac tcgtgcccat caacaacaga gaaagggtga tcgctgaaca catcagagca
     3721 tctcttcccc cctggcgact aaaagtgctc ccaatctcgt cttacatctg atacgttgtt
     3781 caaactccgc aacccgatct gcacactgcc ctgccttggc ccttcttgga gtctccgctt
     3841 ccccacgcca tctcacgctc gaggactccg ctgctaggta tgagctaggc ctgccgtcga
     3901 caggatccac cagacgtcaa caataaccca tcttggaaat acataagaag acgtcacttc
     3961 cctccctttt cgaaaggaag agatcatcat cagcatccat catggctggt ctttttggtt
     4021 ccgccgccgc atccgcgagc aacaccctcg gcgatcttaa gcaggatgtc gagctcggcc
     4081 aaccccccga ggacagcatc agcgaccttg ccttcaaccc caaccccgcc gaccagaagg
     4141 acttcctcgc cgtagccagc tgggacaaga agacacgcat ttacgagatc ttgagtaatg
     4201 gtcaaggtca gggacaggcc atgattgagc atgatgcccc cgttttctct tgcgatttct
     4261 tcaaggtatg tttgcgtcga ttggcctggc aggtagcccg acaaaacact gacatgcact
     4321 tctcccaaca ggacggcaca aaggtcattt ccgccggcgc cgacaaggcc gccaaggttc
     4381 tcgatttggc caccggtcag tcgatgcagg tcgctgctca cgatatgccc atcaagtgcg
     4441 ttcgctactt cgaggccaat ggcacgccca tggcggtgac aggtggctgg gacaagcaga
     4501 tcaagtactg ggatttccgc tccgcgaacc cggccgccac ggtgcaggcc caggagcgcg
     4561 tttataccat ggatgtgcgc gacaacttgc tcgtggtcgg aaccgctgat cgctatatca
     4621 acgtgatcaa cctgaaggac cccggcaagt tctacaagac aatgcagagc cctcttaaat
     4681 ggcagactcg tgtggtcagc tgcttcaacg attctcaggg ctttgctatc ggctcgatcg
     4741 agggtagatg cgccattcaa tatgtggagg acaaggattc tgcgtacgcc ttcacctcct
     4801 ccccctttac tatcccatct gccgaatacg ctgactaact gacttgtttt ccagctccaa
     4861 cttctctttc aaatgccacc gcgaccccgc ccaaggcaac accacggccg tccacgctgt
     4921 caacgacatc tccttccacc cccaacacgg caccttcagc acagccggta gcgacggcac
     4981 cttccacttc tgggacaagg acgccaagca ccgtctcaag ggttacccca acgtcggcgg
     5041 cagcatcacc tcgacaacgt tcaacaagac cggcagcatc tttgcctacg ccattagtta
     5101 tgactggagc aaggggtacc aaggcaactc gcccacgtac ccgaccaagg tcatgctcca
     5161 cccagtacag caggacgagt gcaagccccg tccgagcgtt aagaagaggg ctggtattgg
     5221 tttggctatt gctggcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     5281 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     5341 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     5401 cgtgccctgg cccaccctcg tgaccacctt cacctacggc gtgcagtgct tcagccgcta
     5461 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     5521 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     5581 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     5641 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     5701 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     5761 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5821 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5881 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5941 cgagctgtac aagtgactag aggatccgac actatagaat acctagacag tttgcaggtc
     6001 atgtgaatgt tatcgccaac acattgaatg ctaaacagat tacctccaac gcctagatga
     6061 aaccaaatta cccttgcctt cttttttttt acgccgttaa tgcagttgtg atagagacca
     6121 tgcaacgtct tctaaacgga gacacaaaca cattcgagtc gatcagatcg aaatgacttg
     6181 ttcctgtacc tcgtcaccat ccagccaccg tctgaaatag acgcagttgc tggggctgac
     6241 aggtccgcct tcctcgcaca tatcatagac agggcaccgc ccacaaggcg cctccataag
     6301 gccacttgca ctcggctcaa ctcccataaa tgaggtaaca atgcctcctt tggttggatc
     6361 atggtcgagg cgtcgttgat tgcacgtcct cggatcctgt ttggggatgc gcgtcacacg
     6421 gtaccccttg cgcccattga ccttgacagg ctctactaag ccatcgtagt atagcacatc
     6481 caccaactgc tggatatccg cctcgctcaa cgtcgtatta ttcgtgatgc cggcgtggtg
     6541 gatgaacttt gcaatgtccg acacagtcgg gtacgacttg taaccggccg ggagaggcag
     6601 caattgcggc ttgccgggcc tggtcttctt ggccggtgcc ggtgccgccg tgtcgtcgtt
     6661 ggaaatctcg cccgcgctgc gcttcgtccc cttggaaata tcctccgagt ggggaatggt
     6721 cccctgaacc acgcccttct tgggattctt ttcacgctgc tgtgctcctc ctccttgtgc
     6781 tcctactcct ggtactccag caccgccgcc gtgggaagta tatgtcgatt tctgcttgat
     6841 aaagtcaaac acaatttcct gtagctcgtt gatgaaggcc gtgtcgagct cgccctcagt
     6901 aaaccagggg ccacctgtgg cgcgatcgga gggacgtagg ttcgccttga tgtacatctt
     6961 cttgttgggg tgctcgacgt tcttcatgct ggcgatgtag cccttttgct cgagatactt
     7021 gatgcaatgc ttcataagcg agtcggccat gttgagcttg ttcttgatgg tccggttcca
     7081 gatgccgtcg gccccggctt cgtcgaccat ggcgtatacc atcattattt cggagttggg
     7141 gagggaggtg taccttttgg gaggaagggg aggttagttg gtgtgctcgg ttggctgttt
     7201 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg aagcataaag
     7261 tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt gcgctcactg
     7321 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7381 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7441 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7501 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7561 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7621 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7681 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7741 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7801 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7861 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7921 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7981 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     8041 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8101 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8161 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8221 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8281 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8341 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8401 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8461 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8521 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8581 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8641 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8701 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8761 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8821 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8881 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8941 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9001 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9061 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9121 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9181 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9241 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9301 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat ctgtgcttca
     9361 ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     9421 catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc
     9481 ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     9541 agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc
     9601 tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca
     9661 tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt
     9721 gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata
     9781 aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt
     9841 tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt
     9901 gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt
     9961 ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt
    10021 cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac
    10081 ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag
    10141 gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt
    10201 ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt ttggtttttt
    10261 gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc
    10321 tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga
    10381 aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg
    10441 ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg
    10501 tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat
    10561 cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg
    10621 ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg
    10681 atcatactaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10741 gccctttcgt c
//
