LOCUS       pXD70LacZ2-Pdadh-LacZ        9717 bp    DNA     circular UNA 11-SEP-2022
DEFINITION  .
ACCESSION   urn.local...6f-fak8g9d
VERSION     urn.local...6f-fak8g9d
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      24..95
                     /note="Geneious type: terminator"
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
                     /standard_name="rrnB T1 terminator"
     rep_origin      complement(276..864)
     CDS             join(complement(1827..1895),complement(1035..1826))
                     /transl_table=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /product="β-lactamase"
                     /standard_name="AmpR"
     regulatory      complement(2102..2135)
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     regulatory      complement(2136..2177)
                     /note="Geneious type: terminator"
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
                     /standard_name="tetA/orfL bidirectional terminator"
     regulatory      2265..2651
                     /note="Geneious type: promoter"
                     /standard_name="Pdadh"
     CDS             2652..5726
                     /standard_name="lacZ beta-galactosidase [ Escherichia coli
                     str. K-12 substr. MG1655 ]"
     regulatory      5737..5777
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0012/T7E"
     regulatory      5786..5831
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0011"
     CDS             complement(5894..6688)
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /standard_name="aphA CDS"
     regulatory      7168..7201
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     CDS             complement(7245..8477)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /translation="MAHIAKYKAPSVGHMLAHYRRDRSSLERDNIDPERIKNDLVVGH
                     YTNKDGKLVVGRVEPRDGEPNWGTVESRIERVNEAQKAAGKRATRKDAVVMADVVVTL
                     PDNVRKGDEDRFFRLTYWYLSKKFGIENMMGGYVHKDEVLKDGTPARDHMHVPFTPIL
                     DGRFNYKQMCPRSFYRSMHKELGDYLEGRMGYRPAIELDEETRAQRVYTDKSVDIDKV
                     RCAVDKAVVEPAKDEAARIVAAAREEAAALLNDAETRKAELVTEIADKEGDLAELDSQ
                     LEDVKLDIEDEQDRLECLRQRADGVARDIGELQPIATEVRGWEAAGKAERGAILDNIV
                     VKCDGLASRIRAGVAGMRIKVEELRSRISRPLEYLMRREQPRQQSLNDVLRDAQRAAD
                     AWNRDHAAPQRTYRGRAR"
                     /standard_name=" plasmid recombination
                     protein_hypothetical protein CDS"
     CDS             complement(9037..9678)
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepB"
                     /translation="MAANAGETKVKLWEGICYPENMRDDWQDEIDDILQVPFAYAVHD
                     IDHDQKSKQRKTHAHVIVVWGGNTTRKAIINVLNRLSADGKKCCSSAEPINNIRHAYD
                     YLIHDTASCRKKGKELYPVEARIEGNNFDIGQLEQLSTKDKQDMLFELVGFIMCERFE
                     TINDFTAAALREFPEQYREIIVGYNSILERYCRGNYLNAERKRKCVEQSGAKE"
                     /standard_name="repB CDS"
ORIGIN      
        1 agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa gactgggcct
       61 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca tgccggcggc
      121 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
      181 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
      241 gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1621 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2101 ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata taatgaccct cttgataacc
     2161 caagagggca ttttttagga acggttattt gattattaat ccggcggcaa ctgaaacgat
     2221 tcggatcctg tattactatt ctgaattcgc ggccgcttct agatgcccca ccccctgtga
     2281 gcccgtacat aacgtcgctc gcgccgaccc ctctgtcgac gggttcgatt atacggaaaa
     2341 gatgagcggc cctctttgag gaaactattc tagtttcaga ataatttatg acctacggtt
     2401 aaagtttccc cagttcaaag cactatcgaa tttttcatga tcacaagatc ataagaattt
     2461 atgatgcggt cgaacctgct ttccaacctt tggacgatgc tctgcacccc ctcgcatccg
     2521 tatggtcggc ccaagcgaca cggggacttt gcaccggttc ggagcccgag gcttcacccg
     2581 gcagcccccc tccccgatcg cacgaacgaa gacgagcaag acgagaacga ggagaaagaa
     2641 ggaggaggag tatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact
     2701 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2761 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2821 gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
     2881 gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg
     2941 atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg tttgttccca
     3001 cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg
     3061 aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg
     3121 ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat
     3181 ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt gacggcagtt
     3241 atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc
     3301 ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca
     3361 gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg
     3421 taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg
     3481 gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg
     3541 aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc
     3601 acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc
     3661 ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc
     3721 gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata
     3781 tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc
     3841 cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg
     3901 aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga
     3961 tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct
     4021 ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca
     4081 aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca
     4141 ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc
     4201 cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt
     4261 gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt
     4321 ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga
     4381 ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc
     4441 cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag
     4501 cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca
     4561 tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg
     4621 tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag
     4681 gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc
     4741 tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg
     4801 cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg
     4861 ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt
     4921 ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac
     4981 tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa
     5041 gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt
     5101 accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga
     5161 ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct
     5221 accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata
     5281 caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa
     5341 actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg
     5401 accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg
     5461 gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc
     5521 agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc
     5581 tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg
     5641 acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt cgctaccatt
     5701 accagttggt ctggtgtcaa aaataaacta gtcgactcac actggctcac cttcgggtgg
     5761 gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt
     5821 ttttattatt taattcctgt tataaaaaaa ggatcaattt tgaactctct cccaaagttg
     5881 atcccttaac gatctaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
     5941 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
     6001 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
     6061 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
     6121 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
     6181 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
     6241 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
     6301 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
     6361 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
     6421 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
     6481 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
     6541 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
     6601 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
     6661 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
     6721 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
     6781 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
     6841 catagtatcg acggagccga ttttgaaacc acaattatga tagaatttac aagctataag
     6901 gttattgtcc tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat
     6961 agatatattg ataagaagac tggaatgccc gctccgccgg gattattcga aagcgcatcg
     7021 tccaagcgag accgtctgca ttcggaaatc tcggccggtt cgcgccgagg aagcccatct
     7081 ggctgcatcc caaatcgaca cacaacgcac ggaggccccg gcgatcgccg gggcctccga
     7141 atcatgcgca tcgtggaagc gaagctccgc aaaaaacccc gcttcagcgg ggttttttcg
     7201 cgtcgcacct ccttgtcgtg tattacaggt actactataa cacatcatct agcccgcccg
     7261 cgatacgtcc tctgcggtgc agcgtggtcg cggttccacg catccgccgc ccgctgcgca
     7321 tcgcgcagca cgtcattaag gctctgttgc cttggctgtt ccctgcgcat cagatattcg
     7381 agcgggcgcg atattcgact tctaagctct tccactttga ttcgcattcc cgcaactcct
     7441 gctctgatgc ggctcgcgag tccatcgcac ttaacaacga tgttatcgag gattgcgccg
     7501 cgctcagctt ttcccgcagc ttcccagccc cgaacctcgg tagcgatggg ttgaagctct
     7561 ccaatgtctc gcgcaactcc gtccgctcgt tgccgaagac actccaatcg gtcttgctca
     7621 tcttcgatat cgagcttcac gtcctcgagc tggctgtcaa gctcggcaag gtcaccctcc
     7681 ttgtcggcaa tctcggtaac aagctctgcc ttacgcgttt cggcatcatt cagtaaagcg
     7741 gcggcttctt ccctagccgc cgcgacgatt cgcgccgctt cgtccttcgc aggttcaacc
     7801 acggccttat cgaccgcaca gcgtaccttg tcgatatcca cggacttgtc cgtgtataca
     7861 cgctgcgccc gcgtctcctc gtcaagctcg attgcagggc ggtagcccat gcgcccctct
     7921 aggtaatccc caagctcctt gtgcatgctc cggtagaagc tgcgggggca catctgcttg
     7981 tagttgaacc gtccatctag gatgggcgtg aacggcacgt gcatgtggtc gcgggcgggc
     8041 gtgccatcct tcaacacctc gtccttgtgg acgtacccgc ccatcatgtt ctcgatgccg
     8101 aacttcttcg acaggtacca gtacgtgaga cggaagaacc tgtcctcatc gcccttgcgc
     8161 acattgtcgg gaagcgtcac aacaacgtca gccataacga ccgcatcctt gcgcgtggca
     8221 cgcttccctg cggccttctg ggcctcgttc acgcgctcga tgcggctctc caccgtgccc
     8281 cagttcggct cgccatcgcg cggctccacg cgccccacaa cgagcttgcc gtccttgttc
     8341 gtgtagtggc cgaccaccaa gtcgttcttg atgcgctcgg ggtcgatgtt gtcgcgctcc
     8401 aggctgctgc ggtcgcggcg gtagtgggca agcatgtgcc cgaccgatgg agccttgtat
     8461 ttcgcgatgt gagccatgga aacctccgaa ccgtacagac ttaatcgact aaagtctagc
     8521 acgttagacc ttagctacgc taaggacgtg gcagggccag ccctgcaccc gctcgggcgt
     8581 tccccccgag accccccagc ccttgcccgt tcgcggcttc gctcacggcc ctcgggccat
     8641 cggcgcgagc gccgaacgga gggacttcgc ccctccgaac cacccctcaa tctacctgcg
     8701 gttcggcaca cggcacacgg cactattagg ggggccgtcg tagtggcccc cctgtggtca
     8761 tttgtcattt gtcatttgtc acattatttt tcggacacat tatttttcgg acgtagttag
     8821 cgaaaaataa tgagccgccc gattctgcag gtctcatcca gtcgagacac gggcaaggaa
     8881 agggtcgatg gagcagagcg tccatcgacc cttaatcaag tctggtctat tcggttggcc
     8941 gtcgcgtcaa ttccgcaatc cgtgaacgag tcggcttaga tctgcgcgag cgcagaccac
     9001 gccgccccgt agtcacgcat tgctgcattg acccgactac tctttggcac ctgactgttc
     9061 cacgcacttg cgcttacgtt cggcgttcag atagttcccg cgacaatagc gctcgagaat
     9121 cgagttgtac cccacgatga tttcgcggta ctgctcggga aactccctca gcgccgcagc
     9181 cgtgaaatcg ttgatggtct cgaacctctc gcacatgatg aagccgacca actcgaagag
     9241 catgtcctgc ttgtccttag tcgagagctg ttcgagctga ccaatgtcga agttgtttcc
     9301 ctcgatgcgt gcttcgaccg gatacagctc cttgcccttc ttgcgacagg aagccgtgtc
     9361 gtgaatcaga tagtcatacg catgcctgat gttgttgatt ggctcggctg acgaacagca
     9421 cttcttgccg tccgcgctca atcggttcaa gacgttgatg attgccttgc gtgtcgtgtt
     9481 gccgccccag acaacaatga cgtgcgcatg cgtcttgcgc tgcttgctct tctggtcatg
     9541 gtctatgtcg tgaaccgcat aagcgaaagg cacttgaaga atgtcgtcta tctcgtcctg
     9601 ccagtcatcg cgcatgttct ctgggtagca gatgccctcc cagagcttca ccttcgtctc
     9661 gccagcgttt gccgccatgc tataatctcc ttgtctcgaa tgcccctgct gccgcca
//
