LOCUS       pXD70LacZ6-LacI-Ptac(LacO)-LacZ       10872 bp    DNA     circular UNA 11-SEP-2022
DEFINITION  .
ACCESSION   urn.local...99-fak9ghm
VERSION     urn.local...99-fak9ghm
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      24..95
                     /note="Geneious type: terminator"
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
                     /standard_name="rrnB T1 terminator"
     rep_origin      complement(276..864)
     CDS             join(complement(1827..1895),complement(1035..1826))
                     /transl_table=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /product="β-lactamase"
                     /standard_name="AmpR"
     regulatory      complement(2102..2135)
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     regulatory      complement(2136..2177)
                     /note="Geneious type: terminator"
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
                     /standard_name="tetA/orfL bidirectional terminator"
     regulatory      2282..2359
                     /note="Geneious type: promoter"
                     /gene="<i>lacI</i> (mutant)"
                     /note="In the <i>lacI<sup>q</sup></i> allele, a single
                     base change in the promoter boosts expression of the
                     <i>lacI</i> gene about 10-fold."
                     /standard_name="lacIq promoter"
     CDS             2360..3442
                     /codon_start=1
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ*"
                     /gene="<i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli. </i>This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /product="<i>lac</i> repressor"
                     /transl_table=1
                     /standard_name="lacI"
     regulatory      join(3685..3690,3691..3706,3707..3713)
                     /note="Geneious type: promoter"
                     /note="strong <i>E. coli</i> promoter; hybrid between the
                     <i>trp</i> and <i>lac</i>&nbsp;UV5 promoters"
                     /standard_name="tac promoter"
     protein_bind    3721..3737
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /standard_name="lac operator"
     CDS             3807..6881
                     /standard_name="lacZ beta-galactosidase [ Escherichia coli
                     str. K-12 substr. MG1655 ]"
     regulatory      6892..6932
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0012/T7E"
     regulatory      6941..6986
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0011"
     CDS             complement(7049..7843)
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /standard_name="aphA CDS"
     regulatory      8323..8356
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     CDS             complement(8400..9632)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /translation="MAHIAKYKAPSVGHMLAHYRRDRSSLERDNIDPERIKNDLVVGH
                     YTNKDGKLVVGRVEPRDGEPNWGTVESRIERVNEAQKAAGKRATRKDAVVMADVVVTL
                     PDNVRKGDEDRFFRLTYWYLSKKFGIENMMGGYVHKDEVLKDGTPARDHMHVPFTPIL
                     DGRFNYKQMCPRSFYRSMHKELGDYLEGRMGYRPAIELDEETRAQRVYTDKSVDIDKV
                     RCAVDKAVVEPAKDEAARIVAAAREEAAALLNDAETRKAELVTEIADKEGDLAELDSQ
                     LEDVKLDIEDEQDRLECLRQRADGVARDIGELQPIATEVRGWEAAGKAERGAILDNIV
                     VKCDGLASRIRAGVAGMRIKVEELRSRISRPLEYLMRREQPRQQSLNDVLRDAQRAAD
                     AWNRDHAAPQRTYRGRAR"
                     /standard_name=" plasmid recombination
                     protein_hypothetical protein CDS"
     CDS             complement(10192..10833)
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepB"
                     /translation="MAANAGETKVKLWEGICYPENMRDDWQDEIDDILQVPFAYAVHD
                     IDHDQKSKQRKTHAHVIVVWGGNTTRKAIINVLNRLSADGKKCCSSAEPINNIRHAYD
                     YLIHDTASCRKKGKELYPVEARIEGNNFDIGQLEQLSTKDKQDMLFELVGFIMCERFE
                     TINDFTAAALREFPEQYREIIVGYNSILERYCRGNYLNAERKRKCVEQSGAKE"
                     /standard_name="repB CDS"
ORIGIN      
        1 agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa gactgggcct
       61 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca tgccggcggc
      121 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
      181 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
      241 gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1621 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2101 ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata taatgaccct cttgataacc
     2161 caagagggca ttttttagga acggttattt gattattaat ccggcggcaa ctgaaacgat
     2221 tcggatcctg tattactatt ctgaattcgc ggccgcttct agatacgctg cccgaaattc
     2281 cgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag
     2341 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
     2401 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
     2461 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
     2521 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
     2581 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
     2641 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
     2701 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
     2761 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
     2821 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
     2881 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
     2941 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
     3001 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
     3061 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
     3121 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
     3181 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
     3241 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     3301 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
     3361 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3421 ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg
     3481 cacaattctc atgtttgaca gcttatcatc gactgcacgg tgcaccaatg cttctggcgt
     3541 caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt
     3601 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
     3661 tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa
     3721 ttgtgagcgg ataacaattt cacacaggaa acagccagtc cgtttaggtg ttttcacgag
     3781 cacttcacca acaaggacca tagcatatga ccatgattac ggattcactg gccgtcgttt
     3841 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3901 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3961 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
     4021 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc
     4081 agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc
     4141 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg
     4201 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc
     4261 atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat
     4321 ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgct
     4381 ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg
     4441 acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct
     4501 ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc
     4561 gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca
     4621 ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac
     4681 tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg
     4741 cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg
     4801 gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga
     4861 ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga
     4921 cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc
     4981 attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg
     5041 atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc
     5101 gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc
     5161 cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc
     5221 tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag
     5281 ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc
     5341 ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc
     5401 gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta
     5461 aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg
     5521 tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg
     5581 attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc
     5641 gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt
     5701 tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc
     5761 tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg
     5821 atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg
     5881 ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag
     5941 ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc
     6001 ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc
     6061 tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg
     6121 gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata
     6181 acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga
     6241 aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg
     6301 ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta
     6361 tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg
     6421 aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg
     6481 tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta
     6541 ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct
     6601 tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt
     6661 ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca
     6721 gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata
     6781 tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc cagctgagcg
     6841 ccggtcgcta ccattaccag ttggtctggt gtcaaaaata aactagtcga ctcacactgg
     6901 ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga
     6961 ttattaatcc ggctttttta ttatttaatt cctgttataa aaaaaggatc aattttgaac
     7021 tctctcccaa agttgatccc ttaacgatct aaaacaattc atccagtaaa atataatatt
     7081 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
     7141 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
     7201 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
     7261 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
     7321 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
     7381 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
     7441 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
     7501 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
     7561 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
     7621 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
     7681 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
     7741 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
     7801 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
     7861 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
     7921 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
     7981 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat tatgatagaa
     8041 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
     8101 ctttgcccat tctatagata tattgataag aagactggaa tgcccgctcc gccgggatta
     8161 ttcgaaagcg catcgtccaa gcgagaccgt ctgcattcgg aaatctcggc cggttcgcgc
     8221 cgaggaagcc catctggctg catcccaaat cgacacacaa cgcacggagg ccccggcgat
     8281 cgccggggcc tccgaatcat gcgcatcgtg gaagcgaagc tccgcaaaaa accccgcttc
     8341 agcggggttt tttcgcgtcg cacctccttg tcgtgtatta caggtactac tataacacat
     8401 catctagccc gcccgcgata cgtcctctgc ggtgcagcgt ggtcgcggtt ccacgcatcc
     8461 gccgcccgct gcgcatcgcg cagcacgtca ttaaggctct gttgccttgg ctgttccctg
     8521 cgcatcagat attcgagcgg gcgcgatatt cgacttctaa gctcttccac tttgattcgc
     8581 attcccgcaa ctcctgctct gatgcggctc gcgagtccat cgcacttaac aacgatgtta
     8641 tcgaggattg cgccgcgctc agcttttccc gcagcttccc agccccgaac ctcggtagcg
     8701 atgggttgaa gctctccaat gtctcgcgca actccgtccg ctcgttgccg aagacactcc
     8761 aatcggtctt gctcatcttc gatatcgagc ttcacgtcct cgagctggct gtcaagctcg
     8821 gcaaggtcac cctccttgtc ggcaatctcg gtaacaagct ctgccttacg cgtttcggca
     8881 tcattcagta aagcggcggc ttcttcccta gccgccgcga cgattcgcgc cgcttcgtcc
     8941 ttcgcaggtt caaccacggc cttatcgacc gcacagcgta ccttgtcgat atccacggac
     9001 ttgtccgtgt atacacgctg cgcccgcgtc tcctcgtcaa gctcgattgc agggcggtag
     9061 cccatgcgcc cctctaggta atccccaagc tccttgtgca tgctccggta gaagctgcgg
     9121 gggcacatct gcttgtagtt gaaccgtcca tctaggatgg gcgtgaacgg cacgtgcatg
     9181 tggtcgcggg cgggcgtgcc atccttcaac acctcgtcct tgtggacgta cccgcccatc
     9241 atgttctcga tgccgaactt cttcgacagg taccagtacg tgagacggaa gaacctgtcc
     9301 tcatcgccct tgcgcacatt gtcgggaagc gtcacaacaa cgtcagccat aacgaccgca
     9361 tccttgcgcg tggcacgctt ccctgcggcc ttctgggcct cgttcacgcg ctcgatgcgg
     9421 ctctccaccg tgccccagtt cggctcgcca tcgcgcggct ccacgcgccc cacaacgagc
     9481 ttgccgtcct tgttcgtgta gtggccgacc accaagtcgt tcttgatgcg ctcggggtcg
     9541 atgttgtcgc gctccaggct gctgcggtcg cggcggtagt gggcaagcat gtgcccgacc
     9601 gatggagcct tgtatttcgc gatgtgagcc atggaaacct ccgaaccgta cagacttaat
     9661 cgactaaagt ctagcacgtt agaccttagc tacgctaagg acgtggcagg gccagccctg
     9721 cacccgctcg ggcgttcccc ccgagacccc ccagcccttg cccgttcgcg gcttcgctca
     9781 cggccctcgg gccatcggcg cgagcgccga acggagggac ttcgcccctc cgaaccaccc
     9841 ctcaatctac ctgcggttcg gcacacggca cacggcacta ttaggggggc cgtcgtagtg
     9901 gcccccctgt ggtcatttgt catttgtcat ttgtcacatt atttttcgga cacattattt
     9961 ttcggacgta gttagcgaaa aataatgagc cgcccgattc tgcaggtctc atccagtcga
    10021 gacacgggca aggaaagggt cgatggagca gagcgtccat cgacccttaa tcaagtctgg
    10081 tctattcggt tggccgtcgc gtcaattccg caatccgtga acgagtcggc ttagatctgc
    10141 gcgagcgcag accacgccgc cccgtagtca cgcattgctg cattgacccg actactcttt
    10201 ggcacctgac tgttccacgc acttgcgctt acgttcggcg ttcagatagt tcccgcgaca
    10261 atagcgctcg agaatcgagt tgtaccccac gatgatttcg cggtactgct cgggaaactc
    10321 cctcagcgcc gcagccgtga aatcgttgat ggtctcgaac ctctcgcaca tgatgaagcc
    10381 gaccaactcg aagagcatgt cctgcttgtc cttagtcgag agctgttcga gctgaccaat
    10441 gtcgaagttg tttccctcga tgcgtgcttc gaccggatac agctccttgc ccttcttgcg
    10501 acaggaagcc gtgtcgtgaa tcagatagtc atacgcatgc ctgatgttgt tgattggctc
    10561 ggctgacgaa cagcacttct tgccgtccgc gctcaatcgg ttcaagacgt tgatgattgc
    10621 cttgcgtgtc gtgttgccgc cccagacaac aatgacgtgc gcatgcgtct tgcgctgctt
    10681 gctcttctgg tcatggtcta tgtcgtgaac cgcataagcg aaaggcactt gaagaatgtc
    10741 gtctatctcg tcctgccagt catcgcgcat gttctctggg tagcagatgc cctcccagag
    10801 cttcaccttc gtctcgccag cgtttgccgc catgctataa tctccttgtc tcgaatgccc
    10861 ctgctgccgc ca
//
