LOCUS       pXD70LacZ8.1-Pcadh-LacZ        9616 bp    DNA     circular UNA 11-SEP-2022
DEFINITION  .
ACCESSION   urn.local...10-fak7p9z
VERSION     urn.local...10-fak7p9z
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      24..95
                     /note="Geneious type: terminator"
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
                     /standard_name="rrnB T1 terminator"
     rep_origin      complement(276..864)
     CDS             join(complement(1827..1895),complement(1035..1826))
                     /transl_table=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /product="β-lactamase"
                     /standard_name="AmpR"
     regulatory      complement(1896..2000)
                     /note="Geneious type: promoter"
                     /gene="<i>bla</i>"
                     /standard_name="AmpR promoter"
     regulatory      complement(2102..2135)
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     regulatory      complement(2136..2177)
                     /note="Geneious type: terminator"
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
                     /standard_name="tetA/orfL bidirectional terminator"
     regulatory      2265..2550
                     /note="Geneious type: promoter"
                     /standard_name="Pcadh"
     CDS             2551..5625
                     /standard_name="lacZ beta-galactosidase [ Escherichia coli
                     str. K-12 substr. MG1655 ]"
     regulatory      5636..5676
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0012/T7E"
     regulatory      5685..5730
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0011"
     CDS             complement(5793..6587)
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /standard_name="aphA CDS"
     regulatory      7067..7100
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     CDS             complement(7144..8376)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /translation="MAHIAKYKAPSVGHMLAHYRRDRSSLERDNIDPERIKNDLVVGH
                     YTNKDGKLVVGRVEPRDGEPNWGTVESRIERVNEAQKAAGKRATRKDAVVMADVVVTL
                     PDNVRKGDEDRFFRLTYWYLSKKFGIENMMGGYVHKDEVLKDGTPARDHMHVPFTPIL
                     DGRFNYKQMCPRSFYRSMHKELGDYLEGRMGYRPAIELDEETRAQRVYTDKSVDIDKV
                     RCAVDKAVVEPAKDEAARIVAAAREEAAALLNDAETRKAELVTEIADKEGDLAELDSQ
                     LEDVKLDIEDEQDRLECLRQRADGVARDIGELQPIATEVRGWEAAGKAERGAILDNIV
                     VKCDGLASRIRAGVAGMRIKVEELRSRISRPLEYLMRREQPRQQSLNDVLRDAQRAAD
                     AWNRDHAAPQRTYRGRAR"
                     /standard_name=" plasmid recombination
                     protein_hypothetical protein CDS"
     CDS             complement(8936..9577)
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepB"
                     /translation="MAANAGETKVKLWEGICYPENMRDDWQDEIDDILQVPFAYAVHD
                     IDHDQKSKQRKTHAHVIVVWGGNTTRKAIINVLNRLSADGKKCCSSAEPINNIRHAYD
                     YLIHDTASCRKKGKELYPVEARIEGNNFDIGQLEQLSTKDKQDMLFELVGFIMCERFE
                     TINDFTAAALREFPEQYREIIVGYNSILERYCRGNYLNAERKRKCVEQSGAKE"
                     /standard_name="repB CDS"
ORIGIN      
        1 agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa gactgggcct
       61 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca tgccggcggc
      121 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
      181 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
      241 gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1621 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2101 ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata taatgaccct cttgataacc
     2161 caagagggca ttttttagga acggttattt gattattaat ccggcggcaa ctgaaacgat
     2221 tcggatcctg tattactatt ctgaattcgc ggccgcttct agatgcggac ccccttttac
     2281 tcacatcccc atggcgataa ggctcgttcg agcattgtcg tcagtatagc aattcgccga
     2341 taccgcggcg gcgggcggat ggcgaaatac cactctcggt ggtaggtctt tcatgagctc
     2401 gatagtaggg tttctgacct ggttgaacag agttcgcatc agcggtgggg tgctcaactc
     2461 gcgctcgatc tgcatcatgg ttcgcacgga cgcttggtcc ggcccgctcg ttggagtgcg
     2521 ggtgaaaagg gaaccggcag aggaggagct atgaccatga ttacggattc actggccgtc
     2581 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     2641 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     2701 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
     2761 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac
     2821 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc
     2881 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
     2941 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg
     3001 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct
     3061 gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg
     3121 cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc
     3181 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact
     3241 cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag
     3301 ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc
     3361 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc
     3421 acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat
     3481 cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat
     3541 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg
     3601 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag
     3661 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt
     3721 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg
     3781 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat
     3841 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat
     3901 cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac
     3961 gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc
     4021 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac
     4081 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag
     4141 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc
     4201 gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac
     4261 tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc
     4321 ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc
     4381 gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc
     4441 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac
     4501 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct
     4561 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag
     4621 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca
     4681 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg
     4741 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc
     4801 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
     4861 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
     4921 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc
     4981 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca
     5041 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta
     5101 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat
     5161 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg
     5221 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
     5281 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac
     5341 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac
     5401 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa
     5461 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc
     5521 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg
     5581 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataaactag tcgactcaca
     5641 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc
     5701 cagattatta atccggcttt tttattattt aattcctgtt ataaaaaaag gatcaatttt
     5761 gaactctctc ccaaagttga tcccttaacg atctaaaaca attcatccag taaaatataa
     5821 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     5881 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     5941 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     6001 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     6061 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     6121 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     6181 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     6241 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     6301 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     6361 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     6421 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     6481 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     6541 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     6601 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     6661 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     6721 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatgat
     6781 agaatttaca agctataagg ttattgtcct gggtttcaag cattagtcca tgcaagtttt
     6841 tatgctttgc ccattctata gatatattga taagaagact ggaatgcccg ctccgccggg
     6901 attattcgaa agcgcatcgt ccaagcgaga ccgtctgcat tcggaaatct cggccggttc
     6961 gcgccgagga agcccatctg gctgcatccc aaatcgacac acaacgcacg gaggccccgg
     7021 cgatcgccgg ggcctccgaa tcatgcgcat cgtggaagcg aagctccgca aaaaaccccg
     7081 cttcagcggg gttttttcgc gtcgcacctc cttgtcgtgt attacaggta ctactataac
     7141 acatcatcta gcccgcccgc gatacgtcct ctgcggtgca gcgtggtcgc ggttccacgc
     7201 atccgccgcc cgctgcgcat cgcgcagcac gtcattaagg ctctgttgcc ttggctgttc
     7261 cctgcgcatc agatattcga gcgggcgcga tattcgactt ctaagctctt ccactttgat
     7321 tcgcattccc gcaactcctg ctctgatgcg gctcgcgagt ccatcgcact taacaacgat
     7381 gttatcgagg attgcgccgc gctcagcttt tcccgcagct tcccagcccc gaacctcggt
     7441 agcgatgggt tgaagctctc caatgtctcg cgcaactccg tccgctcgtt gccgaagaca
     7501 ctccaatcgg tcttgctcat cttcgatatc gagcttcacg tcctcgagct ggctgtcaag
     7561 ctcggcaagg tcaccctcct tgtcggcaat ctcggtaaca agctctgcct tacgcgtttc
     7621 ggcatcattc agtaaagcgg cggcttcttc cctagccgcc gcgacgattc gcgccgcttc
     7681 gtccttcgca ggttcaacca cggccttatc gaccgcacag cgtaccttgt cgatatccac
     7741 ggacttgtcc gtgtatacac gctgcgcccg cgtctcctcg tcaagctcga ttgcagggcg
     7801 gtagcccatg cgcccctcta ggtaatcccc aagctccttg tgcatgctcc ggtagaagct
     7861 gcgggggcac atctgcttgt agttgaaccg tccatctagg atgggcgtga acggcacgtg
     7921 catgtggtcg cgggcgggcg tgccatcctt caacacctcg tccttgtgga cgtacccgcc
     7981 catcatgttc tcgatgccga acttcttcga caggtaccag tacgtgagac ggaagaacct
     8041 gtcctcatcg cccttgcgca cattgtcggg aagcgtcaca acaacgtcag ccataacgac
     8101 cgcatccttg cgcgtggcac gcttccctgc ggccttctgg gcctcgttca cgcgctcgat
     8161 gcggctctcc accgtgcccc agttcggctc gccatcgcgc ggctccacgc gccccacaac
     8221 gagcttgccg tccttgttcg tgtagtggcc gaccaccaag tcgttcttga tgcgctcggg
     8281 gtcgatgttg tcgcgctcca ggctgctgcg gtcgcggcgg tagtgggcaa gcatgtgccc
     8341 gaccgatgga gccttgtatt tcgcgatgtg agccatggaa acctccgaac cgtacagact
     8401 taatcgacta aagtctagca cgttagacct tagctacgct aaggacgtgg cagggccagc
     8461 cctgcacccg ctcgggcgtt ccccccgaga ccccccagcc cttgcccgtt cgcggcttcg
     8521 ctcacggccc tcgggccatc ggcgcgagcg ccgaacggag ggacttcgcc cctccgaacc
     8581 acccctcaat ctacctgcgg ttcggcacac ggcacacggc actattaggg gggccgtcgt
     8641 agtggccccc ctgtggtcat ttgtcatttg tcatttgtca cattattttt cggacacatt
     8701 atttttcgga cgtagttagc gaaaaataat gagccgcccg attctgcagg tctcatccag
     8761 tcgagacacg ggcaaggaaa gggtcgatgg agcagagcgt ccatcgaccc ttaatcaagt
     8821 ctggtctatt cggttggccg tcgcgtcaat tccgcaatcc gtgaacgagt cggcttagat
     8881 ctgcgcgagc gcagaccacg ccgccccgta gtcacgcatt gctgcattga cccgactact
     8941 ctttggcacc tgactgttcc acgcacttgc gcttacgttc ggcgttcaga tagttcccgc
     9001 gacaatagcg ctcgagaatc gagttgtacc ccacgatgat ttcgcggtac tgctcgggaa
     9061 actccctcag cgccgcagcc gtgaaatcgt tgatggtctc gaacctctcg cacatgatga
     9121 agccgaccaa ctcgaagagc atgtcctgct tgtccttagt cgagagctgt tcgagctgac
     9181 caatgtcgaa gttgtttccc tcgatgcgtg cttcgaccgg atacagctcc ttgcccttct
     9241 tgcgacagga agccgtgtcg tgaatcagat agtcatacgc atgcctgatg ttgttgattg
     9301 gctcggctga cgaacagcac ttcttgccgt ccgcgctcaa tcggttcaag acgttgatga
     9361 ttgccttgcg tgtcgtgttg ccgccccaga caacaatgac gtgcgcatgc gtcttgcgct
     9421 gcttgctctt ctggtcatgg tctatgtcgt gaaccgcata agcgaaaggc acttgaagaa
     9481 tgtcgtctat ctcgtcctgc cagtcatcgc gcatgttctc tgggtagcag atgccctccc
     9541 agagcttcac cttcgtctcg ccagcgtttg ccgccatgct ataatctcct tgtctcgaat
     9601 gcccctgctg ccgcca
//
