LOCUS       pXD71Cas10.0-Pct5.1-crRNA(AarI)        7389 bp    DNA     circular UNA 11-SEP-2022
DEFINITION  .
ACCESSION   urn.local...dq-fake7r2
VERSION     urn.local...dq-fake7r2
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      24..95
                     /note="Geneious type: terminator"
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
                     /standard_name="rrnB T1 terminator"
     rep_origin      complement(276..864)
     CDS             join(complement(1827..1895),complement(1035..1826))
                     /transl_table=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /product="β-lactamase"
                     /standard_name="AmpR"
     regulatory      complement(1896..2000)
                     /note="Geneious type: promoter"
                     /gene="<i>bla</i>"
                     /standard_name="AmpR promoter"
     regulatory      complement(2102..2135)
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     regulatory      complement(2136..2177)
                     /note="Geneious type: terminator"
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
                     /standard_name="tetA/orfL bidirectional terminator"
     CDS             complement(2265..2876)
                     /function="<br>"
                     /transl_table=1
                     /gene="<i>cymR </i>from&nbsp;<i>Pseudomonas putida</i>"
                     /codon_start=1
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAA
                     GVSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFF
                     LDDDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDIL
                     WLIFNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR*"
                     /experiment="<br>"
                     /protein_id="<br>"
                     /product="cumate repressor (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/17083727"">Mull
                     ick et al., 2006</a>)"
                     /standard_name="CymR"
     protein_bind    3214..3241
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the
                     <i>p-cmt</i> operon of&nbsp;<i>Pseudomonas putida</i> (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/17083727"">Mull
                     ick et al., 2006</a>)"
                     /allele="<br>"
                     /standard_name="CuO"
     protein_bind    3256..3283
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the
                     <i>p-cmt</i> operon of&nbsp;<i>Pseudomonas putida</i> (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/17083727"">Mull
                     ick et al., 2006</a>)"
                     /allele="<br>"
                     /standard_name="CuO"
     misc_feature    3332..3365
                     /note="Geneious type: Spacer"
                     /standard_name="pseudo-AarI spacer"
     regulatory      3409..3449
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0012/T7E"
     regulatory      3458..3503
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0011"
     CDS             complement(3566..4360)
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /standard_name="aphA CDS"
     regulatory      4840..4873
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     CDS             complement(4917..6149)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /translation="MAHIAKYKAPSVGHMLAHYRRDRSSLERDNIDPERIKNDLVVGH
                     YTNKDGKLVVGRVEPRDGEPNWGTVESRIERVNEAQKAAGKRATRKDAVVMADVVVTL
                     PDNVRKGDEDRFFRLTYWYLSKKFGIENMMGGYVHKDEVLKDGTPARDHMHVPFTPIL
                     DGRFNYKQMCPRSFYRSMHKELGDYLEGRMGYRPAIELDEETRAQRVYTDKSVDIDKV
                     RCAVDKAVVEPAKDEAARIVAAAREEAAALLNDAETRKAELVTEIADKEGDLAELDSQ
                     LEDVKLDIEDEQDRLECLRQRADGVARDIGELQPIATEVRGWEAAGKAERGAILDNIV
                     VKCDGLASRIRAGVAGMRIKVEELRSRISRPLEYLMRREQPRQQSLNDVLRDAQRAAD
                     AWNRDHAAPQRTYRGRAR"
                     /standard_name=" plasmid recombination
                     protein_hypothetical protein CDS"
     CDS             complement(6709..7350)
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepB"
                     /translation="MAANAGETKVKLWEGICYPENMRDDWQDEIDDILQVPFAYAVHD
                     IDHDQKSKQRKTHAHVIVVWGGNTTRKAIINVLNRLSADGKKCCSSAEPINNIRHAYD
                     YLIHDTASCRKKGKELYPVEARIEGNNFDIGQLEQLSTKDKQDMLFELVGFIMCERFE
                     TINDFTAAALREFPEQYREIIVGYNSILERYCRGNYLNAERKRKCVEQSGAKE"
                     /standard_name="repB CDS"
ORIGIN      
        1 agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa gactgggcct
       61 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca tgccggcggc
      121 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
      181 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
      241 gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1621 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2101 ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata taatgaccct cttgataacc
     2161 caagagggca ttttttagga acggttattt gattattaat ccggcggcaa ctgaaacgat
     2221 tcggatcctg tattactatt ctgaattcgc ggccgcttct agatctagcg cttgaatttc
     2281 gcgtaccgct ctcgcgcaat ttcgagtgtc gagttcctga cacgctcaaa gcgttctttg
     2341 tccttctgcc ataggctacg aacagcaagc ccacgcaccg aattgaatat caaccaaagg
     2401 atatcttctg catcatcacg cgaaagacca cggctcacca gaacaccaag ccacatatcc
     2461 tcgacgacaa accgattcct ctctaccgtg cgctgaatac cctcgcgtaa cgctgggtcc
     2521 cggtcggcag ccacaatcaa atcaaggctg atagagaagt catcgtcgag gaaaaattcg
     2581 gcggcgtcgt ccagcatttg ctggatgacg tcatcctctg gcttcaattt cgctaatcga
     2641 gcccgactgc gttcggtgat ctgttcgtaa agccattcaa aagtggcaag cagaagctca
     2701 agctttgtcg ggaaatgatg gctctgcgct cctctggaga caccagcagc accgggcaca
     2761 tctgcgatcc ggaatcccgc gtaacctttt tcccgtaaaa cccccagggc cgctgcaatc
     2821 aacttgccct gggtctccat tgcgcgctct gcctgtgttc ttctctttgg actcatgatc
     2881 accatttttt ttactaaagc ttgatctgca atttgaataa taaccactcc tttgtttatc
     2941 caccgaacta agttggtgtt ttttgaagct tgaattagat atttaaaagt atcatatcta
     3001 atattataac taaattttct aaaaaaaaca ttgaaggaac ggttatttga ttattaatcc
     3061 ggctttttta ttatttaggc aactgaaacg attcggatcc tgtattacta ttctattttg
     3121 tcaaaataat tttattgaca acgtcttatt aacgttgata taatttaaat tttatttgac
     3181 aaaaatgggc tcgtgttgta caataaatgt agtaacaaac agacaatctg gtctgtttgt
     3241 acgagtgttg cggccaacaa acagacaatc tggtctgttt gtattatgat taactaatgt
     3301 cactccccgc atggggagtg cgggttgaaa tagtcgcagg tgcagatctg aactcacctg
     3361 cgactgtcac tccccgcatg gggagtgcgg gttgaaatac tagtcgactc acactggctc
     3421 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
     3481 ttaatccggc ttttttatta tttaattcct gttataaaaa aaggatcaat tttgaactct
     3541 ctcccaaagt tgatccctta acgatctaaa acaattcatc cagtaaaata taatatttta
     3601 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
     3661 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
     3721 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
     3781 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
     3841 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
     3901 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
     3961 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
     4021 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
     4081 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
     4141 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
     4201 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
     4261 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
     4321 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
     4381 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
     4441 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
     4501 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccacaattat gatagaattt
     4561 acaagctata aggttattgt cctgggtttc aagcattagt ccatgcaagt ttttatgctt
     4621 tgcccattct atagatatat tgataagaag actggaatgc ccgctccgcc gggattattc
     4681 gaaagcgcat cgtccaagcg agaccgtctg cattcggaaa tctcggccgg ttcgcgccga
     4741 ggaagcccat ctggctgcat cccaaatcga cacacaacgc acggaggccc cggcgatcgc
     4801 cggggcctcc gaatcatgcg catcgtggaa gcgaagctcc gcaaaaaacc ccgcttcagc
     4861 ggggtttttt cgcgtcgcac ctccttgtcg tgtattacag gtactactat aacacatcat
     4921 ctagcccgcc cgcgatacgt cctctgcggt gcagcgtggt cgcggttcca cgcatccgcc
     4981 gcccgctgcg catcgcgcag cacgtcatta aggctctgtt gccttggctg ttccctgcgc
     5041 atcagatatt cgagcgggcg cgatattcga cttctaagct cttccacttt gattcgcatt
     5101 cccgcaactc ctgctctgat gcggctcgcg agtccatcgc acttaacaac gatgttatcg
     5161 aggattgcgc cgcgctcagc ttttcccgca gcttcccagc cccgaacctc ggtagcgatg
     5221 ggttgaagct ctccaatgtc tcgcgcaact ccgtccgctc gttgccgaag acactccaat
     5281 cggtcttgct catcttcgat atcgagcttc acgtcctcga gctggctgtc aagctcggca
     5341 aggtcaccct ccttgtcggc aatctcggta acaagctctg ccttacgcgt ttcggcatca
     5401 ttcagtaaag cggcggcttc ttccctagcc gccgcgacga ttcgcgccgc ttcgtccttc
     5461 gcaggttcaa ccacggcctt atcgaccgca cagcgtacct tgtcgatatc cacggacttg
     5521 tccgtgtata cacgctgcgc ccgcgtctcc tcgtcaagct cgattgcagg gcggtagccc
     5581 atgcgcccct ctaggtaatc cccaagctcc ttgtgcatgc tccggtagaa gctgcggggg
     5641 cacatctgct tgtagttgaa ccgtccatct aggatgggcg tgaacggcac gtgcatgtgg
     5701 tcgcgggcgg gcgtgccatc cttcaacacc tcgtccttgt ggacgtaccc gcccatcatg
     5761 ttctcgatgc cgaacttctt cgacaggtac cagtacgtga gacggaagaa cctgtcctca
     5821 tcgcccttgc gcacattgtc gggaagcgtc acaacaacgt cagccataac gaccgcatcc
     5881 ttgcgcgtgg cacgcttccc tgcggccttc tgggcctcgt tcacgcgctc gatgcggctc
     5941 tccaccgtgc cccagttcgg ctcgccatcg cgcggctcca cgcgccccac aacgagcttg
     6001 ccgtccttgt tcgtgtagtg gccgaccacc aagtcgttct tgatgcgctc ggggtcgatg
     6061 ttgtcgcgct ccaggctgct gcggtcgcgg cggtagtggg caagcatgtg cccgaccgat
     6121 ggagccttgt atttcgcgat gtgagccatg gaaacctccg aaccgtacag acttaatcga
     6181 ctaaagtcta gcacgttaga ccttagctac gctaaggacg tggcagggcc agccctgcac
     6241 ccgctcgggc gttccccccg agacccccca gcccttgccc gttcgcggct tcgctcacgg
     6301 ccctcgggcc atcggcgcga gcgccgaacg gagggacttc gcccctccga accacccctc
     6361 aatctacctg cggttcggca cacggcacac ggcactatta ggggggccgt cgtagtggcc
     6421 cccctgtggt catttgtcat ttgtcatttg tcacattatt tttcggacac attatttttc
     6481 ggacgtagtt agcgaaaaat aatgagccgc ccgattctgc aggtctcatc cagtcgagac
     6541 acgggcaagg aaagggtcga tggagcagag cgtccatcga cccttaatca agtctggtct
     6601 attcggttgg ccgtcgcgtc aattccgcaa tccgtgaacg agtcggctta gatctgcgcg
     6661 agcgcagacc acgccgcccc gtagtcacgc attgctgcat tgacccgact actctttggc
     6721 acctgactgt tccacgcact tgcgcttacg ttcggcgttc agatagttcc cgcgacaata
     6781 gcgctcgaga atcgagttgt accccacgat gatttcgcgg tactgctcgg gaaactccct
     6841 cagcgccgca gccgtgaaat cgttgatggt ctcgaacctc tcgcacatga tgaagccgac
     6901 caactcgaag agcatgtcct gcttgtcctt agtcgagagc tgttcgagct gaccaatgtc
     6961 gaagttgttt ccctcgatgc gtgcttcgac cggatacagc tccttgccct tcttgcgaca
     7021 ggaagccgtg tcgtgaatca gatagtcata cgcatgcctg atgttgttga ttggctcggc
     7081 tgacgaacag cacttcttgc cgtccgcgct caatcggttc aagacgttga tgattgcctt
     7141 gcgtgtcgtg ttgccgcccc agacaacaat gacgtgcgca tgcgtcttgc gctgcttgct
     7201 cttctggtca tggtctatgt cgtgaaccgc ataagcgaaa ggcacttgaa gaatgtcgtc
     7261 tatctcgtcc tgccagtcat cgcgcatgtt ctctgggtag cagatgccct cccagagctt
     7321 caccttcgtc tcgccagcgt ttgccgccat gctataatct ccttgtctcg aatgcccctg
     7381 ctgccgcca
//
