LOCUS       pXD70Tet(DadRmt2)-DadR(ΔTM)        7877 bp    DNA     circular UNA 11-SEP-2022
DEFINITION  .
ACCESSION   urn.local...ec-fakrds0
VERSION     urn.local...ec-fakrds0
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      24..95
                     /note="Geneious type: terminator"
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
                     /standard_name="rrnB T1 terminator"
     rep_origin      complement(276..864)
     CDS             join(complement(1827..1895),complement(1035..1826))
                     /transl_table=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /product="β-lactamase"
                     /standard_name="AmpR"
     regulatory      complement(1896..2000)
                     /note="Geneious type: promoter"
                     /gene="<i>bla</i>"
                     /standard_name="AmpR promoter"
     regulatory      complement(2102..2135)
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     regulatory      complement(2136..2177)
                     /note="Geneious type: terminator"
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
                     /standard_name="tetA/orfL bidirectional terminator"
     regulatory      2265..2502
                     /note="Geneious type: promoter"
                     /standard_name="PdadR"
     CDS             2503..2829
                     /locus_tag="C1858_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="RDC23576.1"
                     /db_xref="GI:1434256048"
                     /standard_name="DadR (ΔTM)"
     regulatory      2840..2880
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0012/T7E"
     regulatory      2889..2934
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0011"
     CDS             complement(2997..4916)
                     /locus_tag="Eggerthella_lenta_A2_02753"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA
                     sequence:dsm2243_annots2.faa:dsm2243"
                     /codon_start=1
                     /transl_table=11
                     /product="Elen_0960 Prediction=translation elongation
                     factor G Prediction_id=ACV54932.1"
                     /translation="MKIINIGILAHVDAGKTTLTESLLYASGAISEPGSVEKGTTRTD
                     TMFLERQRGITIQAAVTSFQWHRCKVNIVDTPGHMDFLAEVYRSLAVLDGAILVISAK
                     DGVQAQTRILFHALRKMNIPTVIFINKIDQAGVDLQSVVQSVRDKLSADIIIKQTVSL
                     SPEIVLEENTDIEAWDAVIENNDELLEKYIAGEPISREKLAREEQQRVQDASLFPVYH
                     GSAKNGLGIQPLMDAVTGLFQPIGEQGGAALCGSVFKVEYTDCGQRRVYLRLYSGTLR
                     LRDTVALAGREKLKITEMRIPSKGEIVRTDTAYQGEIVILPSDSVRLNDVLGDQTRLP
                     RKRWREDPLPMLRTAIAPKTAAQRERLLDALTQLADTDPLLRCEVDSITHEIILSFLG
                     RVQLEVVSALLSEKYKLETVVKEPSVIYMERPLKAASHTIHIEVPPNPFWASIGLSVT
                     PLSLGSGVQYESRVSLGYLNQSFQNAVRDGIRYGLEQGLFGWNVTDCKICFEYGLYYS
                     PVSTPADFRSLAPIVLEQALKESGTQLLEPYLSFILYAPQEYLSRAYHDAPKYCATIE
                     TAQVKKDEVVFTGEIPARCIQAYRTDLAFYTNGRSVCLTELKGYQAAVGQPVIQPRRP
                     NSRLDKVRHMFQKVM"
                     /standard_name="TetW"
     regulatory      5328..5361
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     CDS             complement(5405..6637)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /translation="MAHIAKYKAPSVGHMLAHYRRDRSSLERDNIDPERIKNDLVVGH
                     YTNKDGKLVVGRVEPRDGEPNWGTVESRIERVNEAQKAAGKRATRKDAVVMADVVVTL
                     PDNVRKGDEDRFFRLTYWYLSKKFGIENMMGGYVHKDEVLKDGTPARDHMHVPFTPIL
                     DGRFNYKQMCPRSFYRSMHKELGDYLEGRMGYRPAIELDEETRAQRVYTDKSVDIDKV
                     RCAVDKAVVEPAKDEAARIVAAAREEAAALLNDAETRKAELVTEIADKEGDLAELDSQ
                     LEDVKLDIEDEQDRLECLRQRADGVARDIGELQPIATEVRGWEAAGKAERGAILDNIV
                     VKCDGLASRIRAGVAGMRIKVEELRSRISRPLEYLMRREQPRQQSLNDVLRDAQRAAD
                     AWNRDHAAPQRTYRGRAR"
                     /standard_name=" plasmid recombination
                     protein_hypothetical protein CDS"
     CDS             complement(7197..7838)
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepB"
                     /translation="MAANAGETKVKLWEGICYPENMRDDWQDEIDDILQVPFAYAVHD
                     IDHDQKSKQRKTHAHVIVVWGGNTTRKAIINVLNRLSADGKKCCSSAEPINNIRHAYD
                     YLIHDTASCRKKGKELYPVEARIEGNNFDIGQLEQLSTKDKQDMLFELVGFIMCERFE
                     TINDFTAAALREFPEQYREIIVGYNSILERYCRGNYLNAERKRKCVEQSGAKE"
                     /standard_name="repB CDS"
ORIGIN      
        1 agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa gactgggcct
       61 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca tgccggcggc
      121 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
      181 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
      241 gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1621 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2101 ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata taatgaccct cttgataacc
     2161 caagagggca ttttttagga acggttattt gattattaat ccggcggcaa ctgaaacgat
     2221 tcggatcctg tattactatt ctgaattcgc ggccgcttct agatgagcat cgtccaaagg
     2281 ttggaaagca ggttcgaccg catcataaat tcttatgatc ttgtgatcat gaaaaattcg
     2341 atagtgcttt gaactgggga aactttaacc gtaggtcata aattattctg aaactagaat
     2401 agtttcctca aagagggccg ctcatctttt ccgtataatc gaacccgtcg acagaggggt
     2461 cggcgcgagc gacgttatgt acgggctcac agggggtggg gcatgacgga gaccgacctc
     2521 atcaaaatcg gcatcggcga ggtcagcctg atgggcacgg tgcccgaaga ggccgccgac
     2581 gacccggccg acccggtgga tccgtgcgcg ctgatcgcgg agcgcttcgc cctgtcgccg
     2641 cgcgagaccg acgtgcttcc gctgctgctg gagggccgca ccatctcgcg catccaggag
     2701 acgctgttca tctcggcggg cacggtgagc acgcatatcc gccacatcta ccagaagacg
     2761 ggcgtggaca atcgccagga gctcatcgac ctggcccacg agacggccgc gcaggacgag
     2821 gcggtctgaa ctagtcgact cacactggct caccttcggg tgggcctttc tgcgtttata
     2881 tactagagag agaatataaa aagccagatt attaatccgg cttttttatt atttaattcc
     2941 tgttataaaa aaaggatcaa ttttgaactc tctcccaaag ttgatccctt aacgatttac
     3001 attaccttct gaaacatatg gcgcaccttg tccaggcggc tgtttggacg gcggggctgg
     3061 atgaccggct gaccgacagc ggcctgatat cctttcagct ctgtaaggca tacgctccgc
     3121 ccgttggtgt aaaaggccag atcagtacgg tatgcctgta tacagcgggc gggaatctcg
     3181 ccagtaaaga caacttcatc cttttttacc tgggccgttt cgatggtggc acagtatttc
     3241 ggtgcatcat gataagccct ggaaaggtat tcctggggcg catagaggat gaaggagaga
     3301 taaggttcca gcagctgcgt ccccgattcc ttcaatgcct gttccaatac aatcggggcc
     3361 aatgagcgga agtccgccgg cgtgctgacc ggactgtaat aaagcccgta ttcaaagcaa
     3421 atcttacagt ccgttacgtt ccagccgaac aagccctgct ccagcccgta acggatacca
     3481 tccctgacag cgttttgaaa actctggttc aagtatccca gcgaaacccg gctctcgtat
     3541 tgtacaccgg agccaagcga gagtggtgta acagacagtc ctatggatgc ccaaaacggg
     3601 ttgggcggca cctcgatatg gatggtgtgg ctggctgctt tgagcggccg ctccatataa
     3661 atgacggagg gttcctttac cactgtttca agcttgtatt tttccgacag caaagcggaa
     3721 acaacctcca actgcacccg gcccaaaaaa gaaagaatga tctcatgggt gatggaatcc
     3781 acttcgcaac gcaaaagcgg gtcagtatcc gcaagttgcg taagagcgtc cagcagccgt
     3841 tctctttgcg ctgccgtttt cggcgcaatc gccgtccgca gcatggggag ggggtcctcg
     3901 cgccaccttt tacgagggag ccgggtttgg tcccctaata catcgtttaa cctcacgctg
     3961 tcgctgggaa ggataacaat ttcaccctga taagcggtgt ctgtccgaac aatttcccct
     4021 ttggatggaa tacgcatctc tgtgattttc agcttttctc tcccggccag ggccaccgta
     4081 tcccgcaggc gcagcgttcc gctgtataac cgtagataga cacgccgctg gccgcaatcg
     4141 gtgtactcaa ccttgaaaac gctgccgcat agggcggcgc ccccctgttc cccaatcggt
     4201 tggaacagcc ctgtcaccgc atccatcaac ggttgaatgc caaggccatt tttggcgctg
     4261 ccatgataga ctgggaacag ggaggcgtct tgaacccgct gctgttcctc ccgcgcaagt
     4321 ttttcccggc tgattggttc tcctgcgata tacttttcca ataattcatc gttattttcg
     4381 atgaccgcat cccatgcttc tatgtcggta ttttcctcca ggactatttc cggggacagc
     4441 gacaccgtct gcttgatgat aatatcggcg gagagcttat cccgaacaga ctgaaccacg
     4501 ctctgcaaat caacgccagc ctggtcgatc ttgttgataa agataacggt gggaatgttc
     4561 attttccgca gggcatggaa cagaatacgg gtctgggcct gcacgccatc tttagcggag
     4621 atcaccaaga tggccccatc taaaacagcc aaagagcggt acacctccgc caaaaaatcc
     4681 atgtggccgg gcgtatccac aatgttaact ttacatctgt gccactggaa ggaagtgact
     4741 gccgcttgaa tggtaatccc acgctgccgc tccaaaaaca tggtgtccgt cctcgttgtc
     4801 cctttttcga cgctccccgg ttctgaaatg gctccgctgg catatagcag gctctccgtc
     4861 aaggtcgtct ttccagcgtc tacatgggca agaattccaa tattgattat tttcatgtga
     4921 ttgtcctccc tttacagccc caaagggcat aaaaatcccc agcagtaaaa tacttttacc
     4981 actggggatt ataagttgcg gacatacaca tatacagcat acacctgttt gtgattgctg
     5041 tttttgggga tatgtcaaaa ttgataaggc aaaagtattc ttaaattggg tacaaaaaac
     5101 taagtcccta caaaaggagc tatcataatc ctttgttccc actatttgat tatagtttta
     5161 tttaagaata ccttgccgca tattttttac tccttttctg gattaaatca ttgtatcaca
     5221 tcagttttag gaaagcaagt acctaaaaga aatttttctt ccccttatat gtaacaatca
     5281 taccggcttc ctagcgttca gaatgttttc tgctgtctgc tgtggtgcgc aaaaaacccc
     5341 gcttcagcgg ggttttttcg cgtcgcacct ccttgtcgtg tattacaggt actactataa
     5401 cacatcatct agcccgcccg cgatacgtcc tctgcggtgc agcgtggtcg cggttccacg
     5461 catccgccgc ccgctgcgca tcgcgcagca cgtcattaag gctctgttgc cttggctgtt
     5521 ccctgcgcat cagatattcg agcgggcgcg atattcgact tctaagctct tccactttga
     5581 ttcgcattcc cgcaactcct gctctgatgc ggctcgcgag tccatcgcac ttaacaacga
     5641 tgttatcgag gattgcgccg cgctcagctt ttcccgcagc ttcccagccc cgaacctcgg
     5701 tagcgatggg ttgaagctct ccaatgtctc gcgcaactcc gtccgctcgt tgccgaagac
     5761 actccaatcg gtcttgctca tcttcgatat cgagcttcac gtcctcgagc tggctgtcaa
     5821 gctcggcaag gtcaccctcc ttgtcggcaa tctcggtaac aagctctgcc ttacgcgttt
     5881 cggcatcatt cagtaaagcg gcggcttctt ccctagccgc cgcgacgatt cgcgccgctt
     5941 cgtccttcgc aggttcaacc acggccttat cgaccgcaca gcgtaccttg tcgatatcca
     6001 cggacttgtc cgtgtataca cgctgcgccc gcgtctcctc gtcaagctcg attgcagggc
     6061 ggtagcccat gcgcccctct aggtaatccc caagctcctt gtgcatgctc cggtagaagc
     6121 tgcgggggca catctgcttg tagttgaacc gtccatctag gatgggcgtg aacggcacgt
     6181 gcatgtggtc gcgggcgggc gtgccatcct tcaacacctc gtccttgtgg acgtacccgc
     6241 ccatcatgtt ctcgatgccg aacttcttcg acaggtacca gtacgtgaga cggaagaacc
     6301 tgtcctcatc gcccttgcgc acattgtcgg gaagcgtcac aacaacgtca gccataacga
     6361 ccgcatcctt gcgcgtggca cgcttccctg cggccttctg ggcctcgttc acgcgctcga
     6421 tgcggctctc caccgtgccc cagttcggct cgccatcgcg cggctccacg cgccccacaa
     6481 cgagcttgcc gtccttgttc gtgtagtggc cgaccaccaa gtcgttcttg atgcgctcgg
     6541 ggtcgatgtt gtcgcgctcc aggctgctgc ggtcgcggcg gtagtgggca agcatgtgcc
     6601 cgaccgatgg agccttgtat ttcgcgatgt gagccatgga aacctccgaa ccgtacagac
     6661 ttaatcgact aaagtctagc acgttagacc ttagctacgc taaggacgtg gcagggccag
     6721 ccctgcaccc gctcgggcgt tccccccgag accccccagc ccttgcccgt tcgcggcttc
     6781 gctcacggcc ctcgggccat cggcgcgagc gccgaacgga gggacttcgc ccctccgaac
     6841 cacccctcaa tctacctgcg gttcggcaca cggcacacgg cactattagg ggggccgtcg
     6901 tagtggcccc cctgtggtca tttgtcattt gtcatttgtc acattatttt tcggacacat
     6961 tatttttcgg acgtagttag cgaaaaataa tgagccgccc gattctgcag gtctcatcca
     7021 gtcgagacac gggcaaggaa agggtcgatg gagcagagcg tccatcgacc cttaatcaag
     7081 tctggtctat tcggttggcc gtcgcgtcaa ttccgcaatc cgtgaacgag tcggcttaga
     7141 tctgcgcgag cgcagaccac gccgccccgt agtcacgcat tgctgcattg acccgactac
     7201 tctttggcac ctgactgttc cacgcacttg cgcttacgtt cggcgttcag atagttcccg
     7261 cgacaatagc gctcgagaat cgagttgtac cccacgatga tttcgcggta ctgctcggga
     7321 aactccctca gcgccgcagc cgtgaaatcg ttgatggtct cgaacctctc gcacatgatg
     7381 aagccgacca actcgaagag catgtcctgc ttgtccttag tcgagagctg ttcgagctga
     7441 ccaatgtcga agttgtttcc ctcgatgcgt gcttcgaccg gatacagctc cttgcccttc
     7501 ttgcgacagg aagccgtgtc gtgaatcaga tagtcatacg catgcctgat gttgttgatt
     7561 ggctcggctg acgaacagca cttcttgccg tccgcgctca atcggttcaa gacgttgatg
     7621 attgccttgc gtgtcgtgtt gccgccccag acaacaatga cgtgcgcatg cgtcttgcgc
     7681 tgcttgctct tctggtcatg gtctatgtcg tgaaccgcat aagcgaaagg cacttgaaga
     7741 atgtcgtcta tctcgtcctg ccagtcatcg cgcatgttct ctgggtagca gatgccctcc
     7801 cagagcttca ccttcgtctc gccagcgttt gccgccatgc tataatctcc ttgtctcgaa
     7861 tgcccctgct gccgcca
//
