LOCUS       pXD70Tet(DadRmt5)-N-3xFLAG-DadR(ΔDBD)        8894 bp    DNA     circular UNA 11-SEP-2022
DEFINITION  .
ACCESSION   urn.local...g2-fakzntm
VERSION     urn.local...g2-fakzntm
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      24..95
                     /note="Geneious type: terminator"
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
                     /standard_name="rrnB T1 terminator"
     rep_origin      complement(276..864)
     CDS             join(complement(1827..1895),complement(1035..1826))
                     /transl_table=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /product="β-lactamase"
                     /standard_name="AmpR"
     regulatory      complement(1896..2000)
                     /note="Geneious type: promoter"
                     /gene="<i>bla</i>"
                     /standard_name="AmpR promoter"
     regulatory      complement(2102..2135)
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     regulatory      complement(2136..2177)
                     /note="Geneious type: terminator"
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
                     /standard_name="tetA/orfL bidirectional terminator"
     regulatory      2265..2502
                     /note="Geneious type: promoter"
                     /standard_name="PdadR"
     CDS             2503..2571
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /standard_name="3xFLAG"
     CDS             2572..2598
                     /standard_name="linker"
     CDS             <2599..3846
                     /locus_tag="C1858_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="RDC23576.1"
                     /db_xref="GI:1434256048"
                     /standard_name="DadR (ΔDBD)"
     regulatory      3857..3897
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0012/T7E"
     regulatory      3906..3951
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0011"
     CDS             complement(4014..5933)
                     /locus_tag="Eggerthella_lenta_A2_02753"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA
                     sequence:dsm2243_annots2.faa:dsm2243"
                     /codon_start=1
                     /transl_table=11
                     /product="Elen_0960 Prediction=translation elongation
                     factor G Prediction_id=ACV54932.1"
                     /translation="MKIINIGILAHVDAGKTTLTESLLYASGAISEPGSVEKGTTRTD
                     TMFLERQRGITIQAAVTSFQWHRCKVNIVDTPGHMDFLAEVYRSLAVLDGAILVISAK
                     DGVQAQTRILFHALRKMNIPTVIFINKIDQAGVDLQSVVQSVRDKLSADIIIKQTVSL
                     SPEIVLEENTDIEAWDAVIENNDELLEKYIAGEPISREKLAREEQQRVQDASLFPVYH
                     GSAKNGLGIQPLMDAVTGLFQPIGEQGGAALCGSVFKVEYTDCGQRRVYLRLYSGTLR
                     LRDTVALAGREKLKITEMRIPSKGEIVRTDTAYQGEIVILPSDSVRLNDVLGDQTRLP
                     RKRWREDPLPMLRTAIAPKTAAQRERLLDALTQLADTDPLLRCEVDSITHEIILSFLG
                     RVQLEVVSALLSEKYKLETVVKEPSVIYMERPLKAASHTIHIEVPPNPFWASIGLSVT
                     PLSLGSGVQYESRVSLGYLNQSFQNAVRDGIRYGLEQGLFGWNVTDCKICFEYGLYYS
                     PVSTPADFRSLAPIVLEQALKESGTQLLEPYLSFILYAPQEYLSRAYHDAPKYCATIE
                     TAQVKKDEVVFTGEIPARCIQAYRTDLAFYTNGRSVCLTELKGYQAAVGQPVIQPRRP
                     NSRLDKVRHMFQKVM"
                     /standard_name="TetW"
     regulatory      6345..6378
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     CDS             complement(6422..7654)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /translation="MAHIAKYKAPSVGHMLAHYRRDRSSLERDNIDPERIKNDLVVGH
                     YTNKDGKLVVGRVEPRDGEPNWGTVESRIERVNEAQKAAGKRATRKDAVVMADVVVTL
                     PDNVRKGDEDRFFRLTYWYLSKKFGIENMMGGYVHKDEVLKDGTPARDHMHVPFTPIL
                     DGRFNYKQMCPRSFYRSMHKELGDYLEGRMGYRPAIELDEETRAQRVYTDKSVDIDKV
                     RCAVDKAVVEPAKDEAARIVAAAREEAAALLNDAETRKAELVTEIADKEGDLAELDSQ
                     LEDVKLDIEDEQDRLECLRQRADGVARDIGELQPIATEVRGWEAAGKAERGAILDNIV
                     VKCDGLASRIRAGVAGMRIKVEELRSRISRPLEYLMRREQPRQQSLNDVLRDAQRAAD
                     AWNRDHAAPQRTYRGRAR"
                     /standard_name=" plasmid recombination
                     protein_hypothetical protein CDS"
     CDS             complement(8214..8855)
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepB"
                     /translation="MAANAGETKVKLWEGICYPENMRDDWQDEIDDILQVPFAYAVHD
                     IDHDQKSKQRKTHAHVIVVWGGNTTRKAIINVLNRLSADGKKCCSSAEPINNIRHAYD
                     YLIHDTASCRKKGKELYPVEARIEGNNFDIGQLEQLSTKDKQDMLFELVGFIMCERFE
                     TINDFTAAALREFPEQYREIIVGYNSILERYCRGNYLNAERKRKCVEQSGAKE"
                     /standard_name="repB CDS"
ORIGIN      
        1 agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa gactgggcct
       61 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca tgccggcggc
      121 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
      181 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
      241 gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1621 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2101 ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata taatgaccct cttgataacc
     2161 caagagggca ttttttagga acggttattt gattattaat ccggcggcaa ctgaaacgat
     2221 tcggatcctg tattactatt ctgaattcgc ggccgcttct agatgagcat cgtccaaagg
     2281 ttggaaagca ggttcgaccg catcataaat tcttatgatc ttgtgatcat gaaaaattcg
     2341 atagtgcttt gaactgggga aactttaacc gtaggtcata aattattctg aaactagaat
     2401 agtttcctca aagagggccg ctcatctttt ccgtataatc gaacccgtcg acagaggggt
     2461 cggcgcgagc gacgttatgt acgggctcac agggggtggg gcatggacta caaagaccat
     2521 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gggaattcta
     2581 cgtagcggcc gcggatccga aaagcttcgg agtgcatggg ataccgcgtt ggggacgagc
     2641 ctttccctca agcgcatcgc gggcgcggcc ttgcaatggc tttggatgct catgatgttc
     2701 tacagcatcg tgccgtacgg cttctcgtcg gacgtgcgcg gctcgctgta cgtcagcctg
     2761 atcatatcgc tggtggccat ggtggtgacc atgctgttcg tctccgtcat cgtgcggcgc
     2821 gagcgggtga gcggcaaccg ctccatcgtg ctgggatcgg ccatcatgat gagcgcgggc
     2881 agcgtgctca cgccgttctc cgatcctacc acgcccgtcg gcatgctggt gctgggcctg
     2941 agcgcggtca tgaccggcac gggctcggcc gtgctgttct tgtgctggat cgagctcgag
     3001 tcggggctgg gaggccgctt ggcgcttgtg gagcttgcct cgtcgctgtg catcgcgttc
     3061 gtcgtggggt tcttgctgat cgtagggccg gcgatacccg tcatcgtgct catcgttgtc
     3121 atgcccgtgc tttcggcggc agctctcagg cgcagcgcgc ccgatacgcc gcagctgccg
     3181 cgcgagcccg agcagccttt gtcgcgccat acggcgaccc tgttcgcgaa ggcgcttctg
     3241 ggcgcggtgc tcatcggcat gttgatgggg ttcttcgacg tggtgtcggg gttcaaaacc
     3301 tacgaggtgc aggacatctt cggcacgtac ctgtttctgg cgggcttcat cggagcgttg
     3361 gccatctgcc tgatagccgt gttcctgcag cgcgacagcg tgttcttctc gtaccgcgtc
     3421 tccatgctga tgctgtgctt gggatgcttg tccacgccgt tcatggccga caacaacacg
     3481 tattcgagcg cgcttatctt cggcggctac cactgcttcg tgctggtgct gtgcgtggtg
     3541 tgcatcgacg tggcgtccag cttccgcgtg agcccggcgc ggacgatcgg cctggggttc
     3601 gtggcgctgt acggcggcga gaccgcaggc cagctgttcg cccattcgct ggaggcgacc
     3661 ggggtcagca tgttcgacct cacgctggtc acgctggtgg ccgtgtcgct gctgttcatc
     3721 gcgcatttgt tcctgttcac ggagaccgac ctcatcaaaa tcggcatcgg cgaggtcagc
     3781 ctgatgggca cggtgcccga agaggccgcc gacgacccgg ccgacccggt ggatccgtgc
     3841 gcgtgaacta gtcgactcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     3901 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt taattcctgt
     3961 tataaaaaaa ggatcaattt tgaactctct cccaaagttg atcccttaac gatttacatt
     4021 accttctgaa acatatggcg caccttgtcc aggcggctgt ttggacggcg gggctggatg
     4081 accggctgac cgacagcggc ctgatatcct ttcagctctg taaggcatac gctccgcccg
     4141 ttggtgtaaa aggccagatc agtacggtat gcctgtatac agcgggcggg aatctcgcca
     4201 gtaaagacaa cttcatcctt ttttacctgg gccgtttcga tggtggcaca gtatttcggt
     4261 gcatcatgat aagccctgga aaggtattcc tggggcgcat agaggatgaa ggagagataa
     4321 ggttccagca gctgcgtccc cgattccttc aatgcctgtt ccaatacaat cggggccaat
     4381 gagcggaagt ccgccggcgt gctgaccgga ctgtaataaa gcccgtattc aaagcaaatc
     4441 ttacagtccg ttacgttcca gccgaacaag ccctgctcca gcccgtaacg gataccatcc
     4501 ctgacagcgt tttgaaaact ctggttcaag tatcccagcg aaacccggct ctcgtattgt
     4561 acaccggagc caagcgagag tggtgtaaca gacagtccta tggatgccca aaacgggttg
     4621 ggcggcacct cgatatggat ggtgtggctg gctgctttga gcggccgctc catataaatg
     4681 acggagggtt cctttaccac tgtttcaagc ttgtattttt ccgacagcaa agcggaaaca
     4741 acctccaact gcacccggcc caaaaaagaa agaatgatct catgggtgat ggaatccact
     4801 tcgcaacgca aaagcgggtc agtatccgca agttgcgtaa gagcgtccag cagccgttct
     4861 ctttgcgctg ccgttttcgg cgcaatcgcc gtccgcagca tggggagggg gtcctcgcgc
     4921 caccttttac gagggagccg ggtttggtcc cctaatacat cgtttaacct cacgctgtcg
     4981 ctgggaagga taacaatttc accctgataa gcggtgtctg tccgaacaat ttcccctttg
     5041 gatggaatac gcatctctgt gattttcagc ttttctctcc cggccagggc caccgtatcc
     5101 cgcaggcgca gcgttccgct gtataaccgt agatagacac gccgctggcc gcaatcggtg
     5161 tactcaacct tgaaaacgct gccgcatagg gcggcgcccc cctgttcccc aatcggttgg
     5221 aacagccctg tcaccgcatc catcaacggt tgaatgccaa ggccattttt ggcgctgcca
     5281 tgatagactg ggaacaggga ggcgtcttga acccgctgct gttcctcccg cgcaagtttt
     5341 tcccggctga ttggttctcc tgcgatatac ttttccaata attcatcgtt attttcgatg
     5401 accgcatccc atgcttctat gtcggtattt tcctccagga ctatttccgg ggacagcgac
     5461 accgtctgct tgatgataat atcggcggag agcttatccc gaacagactg aaccacgctc
     5521 tgcaaatcaa cgccagcctg gtcgatcttg ttgataaaga taacggtggg aatgttcatt
     5581 ttccgcaggg catggaacag aatacgggtc tgggcctgca cgccatcttt agcggagatc
     5641 accaagatgg ccccatctaa aacagccaaa gagcggtaca cctccgccaa aaaatccatg
     5701 tggccgggcg tatccacaat gttaacttta catctgtgcc actggaagga agtgactgcc
     5761 gcttgaatgg taatcccacg ctgccgctcc aaaaacatgg tgtccgtcct cgttgtccct
     5821 ttttcgacgc tccccggttc tgaaatggct ccgctggcat atagcaggct ctccgtcaag
     5881 gtcgtctttc cagcgtctac atgggcaaga attccaatat tgattatttt catgtgattg
     5941 tcctcccttt acagccccaa agggcataaa aatccccagc agtaaaatac ttttaccact
     6001 ggggattata agttgcggac atacacatat acagcataca cctgtttgtg attgctgttt
     6061 ttggggatat gtcaaaattg ataaggcaaa agtattctta aattgggtac aaaaaactaa
     6121 gtccctacaa aaggagctat cataatcctt tgttcccact atttgattat agttttattt
     6181 aagaatacct tgccgcatat tttttactcc ttttctggat taaatcattg tatcacatca
     6241 gttttaggaa agcaagtacc taaaagaaat ttttcttccc cttatatgta acaatcatac
     6301 cggcttccta gcgttcagaa tgttttctgc tgtctgctgt ggtgcgcaaa aaaccccgct
     6361 tcagcggggt tttttcgcgt cgcacctcct tgtcgtgtat tacaggtact actataacac
     6421 atcatctagc ccgcccgcga tacgtcctct gcggtgcagc gtggtcgcgg ttccacgcat
     6481 ccgccgcccg ctgcgcatcg cgcagcacgt cattaaggct ctgttgcctt ggctgttccc
     6541 tgcgcatcag atattcgagc gggcgcgata ttcgacttct aagctcttcc actttgattc
     6601 gcattcccgc aactcctgct ctgatgcggc tcgcgagtcc atcgcactta acaacgatgt
     6661 tatcgaggat tgcgccgcgc tcagcttttc ccgcagcttc ccagccccga acctcggtag
     6721 cgatgggttg aagctctcca atgtctcgcg caactccgtc cgctcgttgc cgaagacact
     6781 ccaatcggtc ttgctcatct tcgatatcga gcttcacgtc ctcgagctgg ctgtcaagct
     6841 cggcaaggtc accctccttg tcggcaatct cggtaacaag ctctgcctta cgcgtttcgg
     6901 catcattcag taaagcggcg gcttcttccc tagccgccgc gacgattcgc gccgcttcgt
     6961 ccttcgcagg ttcaaccacg gccttatcga ccgcacagcg taccttgtcg atatccacgg
     7021 acttgtccgt gtatacacgc tgcgcccgcg tctcctcgtc aagctcgatt gcagggcggt
     7081 agcccatgcg cccctctagg taatccccaa gctccttgtg catgctccgg tagaagctgc
     7141 gggggcacat ctgcttgtag ttgaaccgtc catctaggat gggcgtgaac ggcacgtgca
     7201 tgtggtcgcg ggcgggcgtg ccatccttca acacctcgtc cttgtggacg tacccgccca
     7261 tcatgttctc gatgccgaac ttcttcgaca ggtaccagta cgtgagacgg aagaacctgt
     7321 cctcatcgcc cttgcgcaca ttgtcgggaa gcgtcacaac aacgtcagcc ataacgaccg
     7381 catccttgcg cgtggcacgc ttccctgcgg ccttctgggc ctcgttcacg cgctcgatgc
     7441 ggctctccac cgtgccccag ttcggctcgc catcgcgcgg ctccacgcgc cccacaacga
     7501 gcttgccgtc cttgttcgtg tagtggccga ccaccaagtc gttcttgatg cgctcggggt
     7561 cgatgttgtc gcgctccagg ctgctgcggt cgcggcggta gtgggcaagc atgtgcccga
     7621 ccgatggagc cttgtatttc gcgatgtgag ccatggaaac ctccgaaccg tacagactta
     7681 atcgactaaa gtctagcacg ttagacctta gctacgctaa ggacgtggca gggccagccc
     7741 tgcacccgct cgggcgttcc ccccgagacc ccccagccct tgcccgttcg cggcttcgct
     7801 cacggccctc gggccatcgg cgcgagcgcc gaacggaggg acttcgcccc tccgaaccac
     7861 ccctcaatct acctgcggtt cggcacacgg cacacggcac tattaggggg gccgtcgtag
     7921 tggcccccct gtggtcattt gtcatttgtc atttgtcaca ttatttttcg gacacattat
     7981 ttttcggacg tagttagcga aaaataatga gccgcccgat tctgcaggtc tcatccagtc
     8041 gagacacggg caaggaaagg gtcgatggag cagagcgtcc atcgaccctt aatcaagtct
     8101 ggtctattcg gttggccgtc gcgtcaattc cgcaatccgt gaacgagtcg gcttagatct
     8161 gcgcgagcgc agaccacgcc gccccgtagt cacgcattgc tgcattgacc cgactactct
     8221 ttggcacctg actgttccac gcacttgcgc ttacgttcgg cgttcagata gttcccgcga
     8281 caatagcgct cgagaatcga gttgtacccc acgatgattt cgcggtactg ctcgggaaac
     8341 tccctcagcg ccgcagccgt gaaatcgttg atggtctcga acctctcgca catgatgaag
     8401 ccgaccaact cgaagagcat gtcctgcttg tccttagtcg agagctgttc gagctgacca
     8461 atgtcgaagt tgtttccctc gatgcgtgct tcgaccggat acagctcctt gcccttcttg
     8521 cgacaggaag ccgtgtcgtg aatcagatag tcatacgcat gcctgatgtt gttgattggc
     8581 tcggctgacg aacagcactt cttgccgtcc gcgctcaatc ggttcaagac gttgatgatt
     8641 gccttgcgtg tcgtgttgcc gccccagaca acaatgacgt gcgcatgcgt cttgcgctgc
     8701 ttgctcttct ggtcatggtc tatgtcgtga accgcataag cgaaaggcac ttgaagaatg
     8761 tcgtctatct cgtcctgcca gtcatcgcgc atgttctctg ggtagcagat gccctcccag
     8821 agcttcacct tcgtctcgcc agcgtttgcc gccatgctat aatctccttg tctcgaatgc
     8881 ccctgctgcc gcca
//
