LOCUS       pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)        8451 bp    DNA     circular UNA 10-SEP-2022
DEFINITION  .
ACCESSION   urn.local...aa-fahkmsn
VERSION     urn.local...aa-fahkmsn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      24..95
                     /note="Geneious type: terminator"
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
                     /standard_name="rrnB T1 terminator"
     rep_origin      complement(276..864)
     CDS             <1035..1240
                     /codon_start=3
                     /locus_tag="ELEN_RS02515"
                     /old_locus_tag="Elen_0504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
                     /product="CtkA family protein"
                     /protein_id="WP_009608187.1"
                     /db_xref="GI:497293970"
                     /db_xref="GeneID:40921334"
                     /translation="VSSLEFDECNRALREVMERMDLEAAHQLVRSVPNISDRRKEFYC
                     HMLDARYRVILEAPYSDLVRRGA"
                     /standard_name="CtkA family protein CDS"
     CDS             1237..2028
                     /locus_tag="ELEN_RS02520"
                     /old_locus_tag="Elen_0505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_009608164.1"
                     /db_xref="GI:497293947"
                     /db_xref="GeneID:40921335"
                     /translation="MRESTTTGMISLDGPGGLVYEVGAITYLVREDESFRYTFVPNWP
                     VIDLLEPPLFQGVPGYDLSLRKTEYVRENVTPTFVSERAPSESREGLWQLLDACGMEY
                     LDKIEWLIRTDTRYIGDGLYVRPFEEREVGADVDVADAIAGAANSEQAARAVLSALCR
                     GDALFLNGEPIADSERKVLHDVLLSMYEKAYRAREEKRISGVRAAAERGAYKGRKRKP
                     MDELVLREVVSSYEARELDAEEAAARLGVSVSTFFRRLKELRLQG"
                     /standard_name="recombinase family protein CDS"
     CDS             2315..>3062
                     /locus_tag="ELEN_RS02535"
                     /old_locus_tag="Elen_0507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_009607964.1"
                     /db_xref="GI:497293747"
                     /db_xref="GeneID:40921337"
                     /translation="MANTTQRRSFGTMSRRSFMRLAGVTSAALALTSATAPAALAEEH
                     DSGTVASSDGVQRIRTMCRGCGKMECGVWVTVENGRAIKIEGDESSFASSGNSCSKSQ
                     ASLQACYHPDRLAYPMKRTNPKGDDDPGWVRISWDEALAEAGTKLNEIKEQRGGNSMF
                     SMCGTSRIYCMASALGMQGILNTANTHQAYQICKGPRHVATGMVSARAYSWMATVDRP
                     SVFVQWGGASELSNYDDSCRTTVDAAVKADK"
                     /standard_name="molybdopterin-dependent oxidoreductase
                     CDS"
     regulatory      complement(3164..3197)
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     regulatory      complement(3198..3239)
                     /note="Geneious type: terminator"
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
                     /standard_name="tetA/orfL bidirectional terminator"
     CDS             complement(3327..3938)
                     /function="<br>"
                     /transl_table=1
                     /gene="<i>cymR </i>from&nbsp;<i>Pseudomonas putida</i>"
                     /codon_start=1
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAA
                     GVSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFF
                     LDDDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDIL
                     WLIFNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR*"
                     /experiment="<br>"
                     /protein_id="<br>"
                     /product="cumate repressor (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/17083727""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/17083727"">Mull
                     ick et al., 2006</a>)"
                     /standard_name="CymR"
     misc_feature    4394..4427
                     /note="Geneious type: Spacer"
                     /standard_name="CadR spacer"
     regulatory      4471..4511
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0012/T7E"
     regulatory      4520..4565
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0011"
     CDS             complement(4628..5422)
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /standard_name="aphA CDS"
     regulatory      5902..5935
                     /note="Geneious type: terminator"
                     /note="BBa_B1002"
                     /standard_name="BBa B1002 terminator"
     CDS             complement(5979..7211)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /translation="MAHIAKYKAPSVGHMLAHYRRDRSSLERDNIDPERIKNDLVVGH
                     YTNKDGKLVVGRVEPRDGEPNWGTVESRIERVNEAQKAAGKRATRKDAVVMADVVVTL
                     PDNVRKGDEDRFFRLTYWYLSKKFGIENMMGGYVHKDEVLKDGTPARDHMHVPFTPIL
                     DGRFNYKQMCPRSFYRSMHKELGDYLEGRMGYRPAIELDEETRAQRVYTDKSVDIDKV
                     RCAVDKAVVEPAKDEAARIVAAAREEAAALLNDAETRKAELVTEIADKEGDLAELDSQ
                     LEDVKLDIEDEQDRLECLRQRADGVARDIGELQPIATEVRGWEAAGKAERGAILDNIV
                     VKCDGLASRIRAGVAGMRIKVEELRSRISRPLEYLMRREQPRQQSLNDVLRDAQRAAD
                     AWNRDHAAPQRTYRGRAR"
                     /standard_name=" plasmid recombination
                     protein_hypothetical protein CDS"
     CDS             complement(7771..8412)
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepB"
                     /translation="MAANAGETKVKLWEGICYPENMRDDWQDEIDDILQVPFAYAVHD
                     IDHDQKSKQRKTHAHVIVVWGGNTTRKAIINVLNRLSADGKKCCSSAEPINNIRHAYD
                     YLIHDTASCRKKGKELYPVEARIEGNNFDIGQLEQLSTKDKQDMLFELVGFIMCERFE
                     TINDFTAAALREFPEQYREIIVGYNSILERYCRGNYLNAERKRKCVEQSGAKE"
                     /standard_name="repB CDS"
ORIGIN      
        1 agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa gactgggcct
       61 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca tgccggcggc
      121 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
      181 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
      241 gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagtcgtgt ccagcttgga attcgacgag tgtaatcgag ccctgcgaga
     1081 ggtcatggag cgtatggatt tggaagcggc tcatcagctc gtgcgatccg ttccgaacat
     1141 aagcgatcgg cgcaaggagt tttactgcca tatgctcgat gcgcgctatc gggtcattct
     1201 ggaagccccc tattcggacc tggtgaggag gggcgcatga gggaatcgac gactacgggg
     1261 atgatcagcc tcgatggtcc cgggggcctg gtatacgagg tgggggcgat cacgtacctg
     1321 gtgcgcgagg acgagtcgtt tcgctatacg ttcgttccga attggccggt gatcgatttg
     1381 ctcgagcccc cgcttttcca gggggtgccg gggtacgacc tgtcgttgcg caagacggaa
     1441 tacgtgcgcg agaacgtcac cccgacgttc gtgagcgagc gggcgccgtc cgagagccgc
     1501 gagggcttgt ggcagctcct cgatgcatgc ggcatggagt acctcgacaa gatcgaatgg
     1561 ctcatcagga cggatacgcg ctacatagga gatggcttgt acgttcggcc atttgaagaa
     1621 cgcgaggtcg gtgccgatgt cgacgtggcc gacgcgatcg cgggcgccgc gaactccgag
     1681 caggctgcaa gagcggtgct ttcggcgctg tgccgaggcg atgccctctt tctgaacggc
     1741 gagcctattg ccgacagcga gcgcaaggtg ctgcacgacg tccttctatc catgtacgag
     1801 aaggcgtatc gcgcgcgcga ggagaagcgc atctcgggcg tgcgcgcggc tgccgagcgg
     1861 ggcgcgtaca agggccgcaa gcgcaagccg atggacgaac ttgtgctgcg tgaggtggtt
     1921 tcgagctatg aagcgcgcga gctggatgcc gaagaggcgg cggctcggct cggcgtgagc
     1981 gtctcgacgt ttttccggag actgaaagag cttcggttgc aagggtaatg gctgtgcagc
     2041 atgctcacat ccccatggcg ataaggctcg ttcgagcatt gtcgtcagta tagcaattcg
     2101 ccgataccgc ggcggcgggc ggatggcgaa ataccactct cggtggtagg tctttcatga
     2161 gctcgatagt agggtttctg acctggttga acagagttcg catcagcggt ggggtgctca
     2221 actcgcgctc gatctgcatc atggttcgca cggacgcttg gtccggcccg ctcgttggag
     2281 tgcgggtgaa aagggaaccg gcagaggagg agctatggcg aacaccacgc aaaggcggtc
     2341 gttcggcacc atgagccgtc gcagcttcat gaggctcgca ggggtgacga gcgctgcgct
     2401 tgcgctgacg tcggcaacgg cgccggcggc gcttgccgaa gagcacgaca gcggaacggt
     2461 tgcgtcgtcg gacggcgtgc agcgtattcg cacgatgtgc cgcgggtgcg gcaagatgga
     2521 atgcggcgtg tgggtgacgg tggagaacgg ccgcgccatc aagatcgagg gggacgagag
     2581 ctcgttcgcg tcgtcgggga acagctgcag caagtcgcag gcttcgctgc aggcgtgcta
     2641 ccatcccgat cggctcgcgt atcccatgaa acgcacgaat cccaagggcg acgacgatcc
     2701 gggttgggtg cgcatcagct gggacgaggc tctcgccgaa gccggcacga agctcaacga
     2761 gatcaaggag cagcgcggcg ggaactccat gttctcgatg tgcggcacca gccgcatcta
     2821 ctgcatggcg agcgcgctcg gcatgcaggg cattctgaac acggcgaaca cccatcaggc
     2881 gtaccagatt tgcaagggtc cccgccatgt ggccaccggt atggtgtcgg ctcgtgcgta
     2941 cagctggatg gccacggtcg accggccgag cgtgttcgtg caatggggcg gcgcttcgga
     3001 gctgtccaac tacgatgact cgtgtcgcac gacggtcgat gctgcggtca aggcggacaa
     3061 gccacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3121 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aaggcgaaaa aaccccgccg
     3181 aagcggggtt ttttgcgaaa tataatgacc ctcttgataa cccaagaggg cattttttag
     3241 gaacggttat ttgattatta atccggcggc aactgaaacg attcggatcc tgtattacta
     3301 ttctgaattc gcggccgctt ctagatctag cgcttgaatt tcgcgtaccg ctctcgcgca
     3361 atttcgagtg tcgagttcct gacacgctca aagcgttctt tgtccttctg ccataggcta
     3421 cgaacagcaa gcccacgcac cgaattgaat atcaaccaaa ggatatcttc tgcatcatca
     3481 cgcgaaagac cacggctcac cagaacacca agccacatat cctcgacgac aaaccgattc
     3541 ctctctaccg tgcgctgaat accctcgcgt aacgctgggt cccggtcggc agccacaatc
     3601 aaatcaaggc tgatagagaa gtcatcgtcg aggaaaaatt cggcggcgtc gtccagcatt
     3661 tgctggatga cgtcatcctc tggcttcaat ttcgctaatc gagcccgact gcgttcggtg
     3721 atctgttcgt aaagccattc aaaagtggca agcagaagct caagctttgt cgggaaatga
     3781 tggctctgcg ctcctctgga gacaccagca gcaccgggca catctgcgat ccggaatccc
     3841 gcgtaacctt tttcccgtaa aacccccagg gccgctgcaa tcaacttgcc ctgggtctcc
     3901 attgcgcgct ctgcctgtgt tcttctcttt ggactcatga tcaccatttt ttttactaaa
     3961 gcttgatctg caatttgaat aataaccact cctttgttta tccaccgaac taagttggtg
     4021 ttttttgaag cttgaattag atatttaaaa gtatcatatc taatattata actaaatttt
     4081 ctaaaaaaaa cattgaagga acggttattt gattattaat ccggcttttt tattatttag
     4141 gcaactgaaa cgattcggat cctgtattac tattctattt tgtcaaaata attttattga
     4201 caacgtctta ttaacgttga tataatttaa attttatttg acaaaaatgg gctcgtgttg
     4261 tacaataaat gtagtaacaa acagacaatc tggtctgttt gtacgagtgt tgcggccaac
     4321 aaacagacaa tctggtctgt ttgtattatg attaactaat gtcactcccc gcatggggag
     4381 tgcgggttga aatgggcttg gagagggcgt catcctgttt ctctggagtc actccccgca
     4441 tggggagtgc gggttgaaat actagtcgac tcacactggc tcaccttcgg gtgggccttt
     4501 ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg gcttttttat
     4561 tatttaattc ctgttataaa aaaaggatca attttgaact ctctcccaaa gttgatccct
     4621 taacgatcta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
     4681 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
     4741 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
     4801 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
     4861 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
     4921 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
     4981 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
     5041 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
     5101 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
     5161 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
     5221 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
     5281 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
     5341 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
     5401 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
     5461 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
     5521 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
     5581 atcgacggag ccgattttga aaccacaatt atgatagaat ttacaagcta taaggttatt
     5641 gtcctgggtt tcaagcatta gtccatgcaa gtttttatgc tttgcccatt ctatagatat
     5701 attgataaga agactggaat gcccgctccg ccgggattat tcgaaagcgc atcgtccaag
     5761 cgagaccgtc tgcattcgga aatctcggcc ggttcgcgcc gaggaagccc atctggctgc
     5821 atcccaaatc gacacacaac gcacggaggc cccggcgatc gccggggcct ccgaatcatg
     5881 cgcatcgtgg aagcgaagct ccgcaaaaaa ccccgcttca gcggggtttt ttcgcgtcgc
     5941 acctccttgt cgtgtattac aggtactact ataacacatc atctagcccg cccgcgatac
     6001 gtcctctgcg gtgcagcgtg gtcgcggttc cacgcatccg ccgcccgctg cgcatcgcgc
     6061 agcacgtcat taaggctctg ttgccttggc tgttccctgc gcatcagata ttcgagcggg
     6121 cgcgatattc gacttctaag ctcttccact ttgattcgca ttcccgcaac tcctgctctg
     6181 atgcggctcg cgagtccatc gcacttaaca acgatgttat cgaggattgc gccgcgctca
     6241 gcttttcccg cagcttccca gccccgaacc tcggtagcga tgggttgaag ctctccaatg
     6301 tctcgcgcaa ctccgtccgc tcgttgccga agacactcca atcggtcttg ctcatcttcg
     6361 atatcgagct tcacgtcctc gagctggctg tcaagctcgg caaggtcacc ctccttgtcg
     6421 gcaatctcgg taacaagctc tgccttacgc gtttcggcat cattcagtaa agcggcggct
     6481 tcttccctag ccgccgcgac gattcgcgcc gcttcgtcct tcgcaggttc aaccacggcc
     6541 ttatcgaccg cacagcgtac cttgtcgata tccacggact tgtccgtgta tacacgctgc
     6601 gcccgcgtct cctcgtcaag ctcgattgca gggcggtagc ccatgcgccc ctctaggtaa
     6661 tccccaagct ccttgtgcat gctccggtag aagctgcggg ggcacatctg cttgtagttg
     6721 aaccgtccat ctaggatggg cgtgaacggc acgtgcatgt ggtcgcgggc gggcgtgcca
     6781 tccttcaaca cctcgtcctt gtggacgtac ccgcccatca tgttctcgat gccgaacttc
     6841 ttcgacaggt accagtacgt gagacggaag aacctgtcct catcgccctt gcgcacattg
     6901 tcgggaagcg tcacaacaac gtcagccata acgaccgcat ccttgcgcgt ggcacgcttc
     6961 cctgcggcct tctgggcctc gttcacgcgc tcgatgcggc tctccaccgt gccccagttc
     7021 ggctcgccat cgcgcggctc cacgcgcccc acaacgagct tgccgtcctt gttcgtgtag
     7081 tggccgacca ccaagtcgtt cttgatgcgc tcggggtcga tgttgtcgcg ctccaggctg
     7141 ctgcggtcgc ggcggtagtg ggcaagcatg tgcccgaccg atggagcctt gtatttcgcg
     7201 atgtgagcca tggaaacctc cgaaccgtac agacttaatc gactaaagtc tagcacgtta
     7261 gaccttagct acgctaagga cgtggcaggg ccagccctgc acccgctcgg gcgttccccc
     7321 cgagaccccc cagcccttgc ccgttcgcgg cttcgctcac ggccctcggg ccatcggcgc
     7381 gagcgccgaa cggagggact tcgcccctcc gaaccacccc tcaatctacc tgcggttcgg
     7441 cacacggcac acggcactat taggggggcc gtcgtagtgg cccccctgtg gtcatttgtc
     7501 atttgtcatt tgtcacatta tttttcggac acattatttt tcggacgtag ttagcgaaaa
     7561 ataatgagcc gcccgattct gcaggtctca tccagtcgag acacgggcaa ggaaagggtc
     7621 gatggagcag agcgtccatc gacccttaat caagtctggt ctattcggtt ggccgtcgcg
     7681 tcaattccgc aatccgtgaa cgagtcggct tagatctgcg cgagcgcaga ccacgccgcc
     7741 ccgtagtcac gcattgctgc attgacccga ctactctttg gcacctgact gttccacgca
     7801 cttgcgctta cgttcggcgt tcagatagtt cccgcgacaa tagcgctcga gaatcgagtt
     7861 gtaccccacg atgatttcgc ggtactgctc gggaaactcc ctcagcgccg cagccgtgaa
     7921 atcgttgatg gtctcgaacc tctcgcacat gatgaagccg accaactcga agagcatgtc
     7981 ctgcttgtcc ttagtcgaga gctgttcgag ctgaccaatg tcgaagttgt ttccctcgat
     8041 gcgtgcttcg accggataca gctccttgcc cttcttgcga caggaagccg tgtcgtgaat
     8101 cagatagtca tacgcatgcc tgatgttgtt gattggctcg gctgacgaac agcacttctt
     8161 gccgtccgcg ctcaatcggt tcaagacgtt gatgattgcc ttgcgtgtcg tgttgccgcc
     8221 ccagacaaca atgacgtgcg catgcgtctt gcgctgcttg ctcttctggt catggtctat
     8281 gtcgtgaacc gcataagcga aaggcacttg aagaatgtcg tctatctcgt cctgccagtc
     8341 atcgcgcatg ttctctgggt agcagatgcc ctcccagagc ttcaccttcg tctcgccagc
     8401 gtttgccgcc atgctataat ctccttgtct cgaatgcccc tgctgccgcc a
//
