LOCUS       pJH4260                 7414 bp    DNA        circular     30-AUG-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             1532..3151
                     /locus_tag="GCaMP6"
                     /label="GCaMP6"
                     /ApEinfo_label="GCaMP6"
                     /ApEinfo_fwdcolor="#ffa1fe"
                     /ApEinfo_revcolor="#ffaadc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3882..3932
                     /locus_tag="intron"
                     /label="intron"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3164..3859
                     /locus_tag="wrmScarlet"
                     /label="wrmScarlet"
                     /ApEinfo_label="wrmScarlet"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     UTR             4060..4794
                     /locus_tag="unc-54 3UTR"
                     /label="unc-54 3UTR"
                     /ApEinfo_label="unc-54 3UTR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     exon            4543..4697
                     /locus_tag="aex-5 exon1"
                     /label="aex-5 exon1"
                     /ApEinfo_label="aex-5 exon1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      6219..6901
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7269..7289
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5462..6121
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          4009..4059
                     /locus_tag="intron(1)"
                     /label="intron(1)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    7241..7263
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="cornflower blue"
                     /ApEinfo_revcolor="cornflower blue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3860..3862
                     /locus_tag="ajout STOP"
                     /label="ajout STOP"
                     /ApEinfo_label="ajout STOP"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1628..1630
                     /locus_tag="ATG"
                     /label="ATG"
                     /ApEinfo_label="ATG"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    27..1514
                     /locus_tag="Ptwk-40"
                     /label="Ptwk-40"
                     /ApEinfo_label="Ptwk-40"
                     /ApEinfo_fwdcolor="#bbffa8"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    27..56
                     /locus_tag="OZM3543"
                     /label="OZM3543"
                     /ApEinfo_label="OZM3543"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AAGCTTGGGC TGCAgcatgc ggccgctcta atcactatca cgtgggatct ggataaattt
       61 tctccaaatt tctcataaaa aatggaaaaa aagaaaagga aatccggttt gtgtatttcc
      121 attttcaatt tgatgcatta aaccagaact tcgttaattt cccttctctt ctccctcaag
      181 cgtgcacact ttttttagat gagaagggct ctatttcgat tggttgtttt tgttttgaac
      241 cagatttttt tttcacaaaa ataaaatttg gaaccttttg ccagtttaat aaagtttttt
      301 gttgggaatg ttcctaaaaa tttttaattt atctaatata aaacttgata gaaacattaa
      361 ttaacctgct tttttgcatt tatttccaac tgaattaaaa taaatgtgaa ttttttttta
      421 ctatgtaaat aaatcgaaaa ttctggttcc tgattctcaa gaccgcatct caacttttca
      481 gccggtaatt tgtccgagag gagtccaatt ttcaaatttt tgatcaaaat gtctactttg
      541 atcgacatcc tccttcttct gcaaggtgtg tatcatcgtc ttgaaatttc aaattcccta
      601 agggaacgaa aagcttgtct cttttggcag attcgattcg atttcacctc tttctccccc
      661 ctcctgccta caatgttgaa agggtcccct ccttgtgctc caaattgctc ataaactcct
      721 ccatactgga cgcttctttt tgcggaaatt ggagtcccag atatattgga agagataaac
      781 ggttttcggg tttgcagttg ttgccatttt cactgatttt tattcaagta gtactacttg
      841 aagttttgtt cctgagtaaa attttatagg ttccatgatt tatgaaattg ttctatagta
      901 gcaccaaact tttcagaact tttttgtttt ttttttcaat tttacagtaa aaagttattc
      961 tcaaatgttt tctcaatttt tgccccttat taactcacgt acgtatgtag tttacagagc
     1021 gtatgtagat gtagttccag tttttggcac ttataatttt agaagtcgac cgataataaa
     1081 tgtttaggca tgccaaatca tcataagcta ctcaaaaaat agaaaatttt tgatatgtag
     1141 caattttcta aaactttatc taaaaatctt tttttccaaa aaatgccttt ggttaataaa
     1201 ttgtaaatca aaattcagca acaaaaaacc tcactggaaa agtgggagta aaaatacgaa
     1261 ataaaatttt cgtaactttt tgtcaaaaca gaatcttaaa tttcccacta tacccatttg
     1321 tccttcaaaa atgccattta aagtaagcaa agaggtatca taaattctca cagaacgtat
     1381 gtagtttttg tgtcctttac ttctccattg cctcatattt tcactttttt cgtggccgca
     1441 gctcacagtg cacacatcac agaatgttcc agagccgaat gattttggat ggaatatcga
     1501 gtgatgaata ttcacccggg ggatccCCGG GATTGGCCAA AGGACCCAAA Ggtatgtttc
     1561 gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGCTAGC GTCGACGGAT
     1621 CCAAAAAATG GGATCTCACC ACCACCACCA CCACGGAATG GCCTCCATGA CCGGAGGACA
     1681 GCAGATGGGA CGTGACCTCT ACGACGACGA CGACAAGGAC CTCGCCACTA TGGTCGACTC
     1741 CTCCCGTCGT AAGTGGAACA AGACCGGACA CGCCGTCCGT GCCATCGGAC GTCTCTCCTC
     1801 CCTCGAGAAC GTCTACATCA AGGCCGACAA GCAGAAGAAC GGAATCAAGG TAAGTTTAAA
     1861 CATGATTTTA CTAACTAACT AATCTGATTT AAATTTTCAG GCCAACTTCC ACATCCGTCA
     1921 CAACATCGAG GACGGAGGAG TCCAGCTCGC CTACCACTAC CAGCAGAACA CCCCAATCGG
     1981 AGACGGACCA GTCCTCCTCC CAGACAACCA CTACCTCTCC GTCCAGTCCA AGCTCTCCAA
     2041 GGACCCAAAC GAGAAGCGTG ACCACATGGT CCTCCTTGAG TTCGTCACCG CCGCCGGAAT
     2101 CACCCTCGGA ATGGACGAGC TCTACAAGGG AGGAACCGGA GGTTCCATGG TCTCCAAGGG
     2161 AGAGGAGCTC TTCACCGGAG TCGTCCCAAT CCTCGTCGAG CTCGACGGAG ACGTCAACGG
     2221 ACACAAGTTC TCCGTCTCTG GAGAGGTAAG TTTAAACATG ATTTTACTAA CTAACTAATC
     2281 TGATTTAAAT TTTCAGGGAG AGGGAGACGC CACCTACGGA AAGCTCACCC TCAAGTTCAT
     2341 CTGCACCACC GGAAAGCTCC CAGTCCCGTG GCCGACCCTC GTCACCACCC TCACCTACGG
     2401 AGTCCAGTGC TTCTCCCGTT ACCCAGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC
     2461 CATGCCAGAG GGATACATCC AGGAGCGTAC CATCTTCTTC AAGGACGACG GAAACTACAA
     2521 GACCCGTGCC GAGGTCAAGT TCGAGGGAGA CACCCTCGTC AACCGTATCG AGCTCAAGGG
     2581 AATCGACTTC AAGGAGGACG GAAACATCCT CGGACACAAG CTTGAGTACA ACCTCCCAGA
     2641 CCAGCTCACC GAGGAGCAGA TCGCCGAGTT CAAGGTAAGT TTAAACATGA TTTTACTAAC
     2701 TAACTAATCT GATTTAAATT TTCAGGAGGC CTTCTCCCTC TTCGACAAGG ACGGAGACGG
     2761 AACCATCACC ACCAAGGAGC TCGGAACCGT CATGCGTTCC CTCGGACAGA ACCCAACCGA
     2821 GGCCGAGCTC CAGGACATGA TCAACGAGGT CGACGCCGAC GGAGACGGAA CCATCGACTT
     2881 CCCAGAGTTC CTCACCATGA TGGCCCGTAA GATGAAGTAC CGTGACACCG AGGAGGAGAT
     2941 CCGTGAGGCC TTCGGAGTCT TCGACAAGGA CGGAAACGGA TACATCTCCG CCGCCGAGCT
     3001 CCGTCACGTC ATGACCAACC TCGGAGAGAA GCTCACCGAC GAGGAGGTCG ACGAGATGAT
     3061 CCGTGAGGCC GACATCGACG GAGACGGACA GGTCAACTAC GAGGAGTTCG TCCAGATGAT
     3121 GACCGCCAAG GGAGGACGTG GAGGATCTGG CGGCGCGCCA AAAATGGTCA GCAAGGGAGA
     3181 GGCAGTTATC AAGGAGTTCA TGCGTTTCAA GGTCCACATG GAGGGATCCA TGAACGGACA
     3241 CGAGTTCGAG ATCGAGGGAG AGGGAGAGGG ACGTCCATAC GAGGGAACCC AAACCGCCAA
     3301 GCTCAAGGTC ACCAAGGGAG GACCACTCCC ATTCTCCTGG GACATCCTCT CCCCACAATT
     3361 CATGTACGGA TCCCGTGCCT TCACCAAGCA CCCAGCCGAC ATCCCAGACT ACTACAAGCA
     3421 ATCCTTCCCA GAGGGATTCA AGTGGGAGCG TGTCATGAAC TTCGAGGACG GAGGAGCCGT
     3481 CACCGTCACC CAAGACACCT CCCTCGAGGA CGGAACCCTC ATCTACAAGG TCAAGCTCCG
     3541 TGGAACCAAC TTCCCACCAG ACGGACCAGT CATGCAAAAG AAGACCATGG GATGGGAGGC
     3601 CTCCACCGAG CGTCTCTACC CAGAGGACGG AGTCCTCAAG GGAGACATCA AGATGGCCCT
     3661 CCGTCTCAAG GACGGAGGAC GTTACCTCGC CGACTTCAAG ACCACCTACA AGGCCAAGAA
     3721 GCCAGTCCAA ATGCCAGGAG CCTACAACGT CGACCGTAAG CTCGACATCA CCTCCCACAA
     3781 CGAGGACTAC ACCGTCGTCG AGCAATACGA GCGTTCCGAG GGACGTCACT CCACCGGAGG
     3841 AATGGACGAG CTCTACAAGT AAGCTCAGCA TTCGTAGAAT Tgtaagttta aactttctca
     3901 tactaactaa cgcgctctat ttaaattttc agAATTCCAA CTGAGCGCCG GTCGCTACCA
     3961 TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac
     4021 tgagttctac taactaacga gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG
     4081 ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC
     4141 CCCTATTTTT GTTATTATCA AAAAAACTTC TTCTTAATTT CTTTGTTTTT TAGCTTCTTT
     4201 TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA
     4261 AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC
     4321 AATGTTCTGT GTACACTTCT TATGTTTTTT TTACTTCTGA TAAATTTTTT TTGAAACATC
     4381 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA
     4441 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA
     4501 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG
     4561 CTTTTGCTTT TTGGGGGTTT CCCCTATTGT TTGTCAAGAG TTTCGAGGAC GGCGTTTTTC
     4621 TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT
     4681 TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG
     4741 GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT
     4801 AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA
     4861 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA
     4921 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA
     4981 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA
     5041 AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT
     5101 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG
     5161 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA
     5221 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT
     5281 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA
     5341 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA
     5401 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT
     5461 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA
     5521 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT
     5581 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC
     5641 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT
     5701 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA
     5761 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC
     5821 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT
     5881 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG
     5941 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC
     6001 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC
     6061 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG
     6121 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA
     6181 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG
     6241 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
     6301 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
     6361 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
     6421 AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
     6481 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
     6541 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
     6601 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
     6661 CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
     6721 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
     6781 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
     6841 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
     6901 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC
     6961 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG
     7021 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA
     7081 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA
     7141 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC
     7201 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA
     7261 ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc
     7321 ttactaacta actattctca tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC
     7381 AACGATGGAT ACGCTAACAA CTTGGAAATG AAAT  
//
